Name

ASAHI, Toru

Official Title

Professor

Affiliation

(School of Advanced Science and Engineering)

Contact Information

Mail Address

Mail Address
tasahi@waseda.jp

Address・Phone Number・Fax Number

Address
2-2,Wakamatsucho,Shinjyuku-ku,Tokyo

URL

Web Page URL

http://asahi-lab.jp/index.html

Grant-in-aids for Scientific Researcher Number
80222595

Sub-affiliation

Sub-affiliation

Faculty of Science and Engineering(Graduate School of Advanced Science and Engineering)

Research Council (Research Organization)/Affiliated organization(Global Education Center)

Faculty of International Research and Education(School of International Liberal Studies)

Affiliated Institutes

欧州バイオメディカルサイエンス研究所

プロジェクト研究所所長 2009-2011

理工学総合研究センター

兼任研究員 2003-2006

ビジネス・テクニカル・コミュニケーション研究所

研究所員 2013-2016

欧州バイオメディカルサイエンス研究所

研究所員 2009-2011

ライフサポートイノベーション研究所

研究所員 2014-2014

実践的ナノ化学G

研究所員 2007-

知的財産拠点形成研究所

研究所員 2009-2013

欧州バイオメディカルサイエンス研究所

プロジェクト研究所所長 2011-2012

欧州バイオメディカルサイエンス研究所

研究所員 2011-2012

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2012-

東日本大震災復興研究拠点・先端環境医工科学研究所

研究所員 2011-2013

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2015-2016

欧州バイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2012-2014

規範科学総合研究所

研究所員 2014-2014

地域間研究所

研究所員 2015-2015

先端ベンチャー・起業家研究所

研究所員 2015-2018

ビジネス・テクニカル・コミュニケーション研究所

研究所員 2016-

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2016-2017

欧州バイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2015-2016

グローバルバイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2017-2018

グローバルバイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2016-2017

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2017-2018

各務記念材料技術研究所

兼任研究員 2017-2018

理工学術院総合研究所(理工学研究所)

兼任研究員 2006-2018

理工学術院総合研究所(理工学研究所)

兼任研究員 2018-

知的財産法制研究所

研究所員 2014-2019

知的財産法制研究所

研究所員 2019-

ライフサポートイノベーション研究所

研究所員 2015-2019

ライフサポートイノベーション研究所

研究所員 2019-

規範科学総合研究所

研究所員 2015-2019

規範科学総合研究所

研究所員 2019-

グローバル科学知融合研究所

プロジェクト研究所所長 2019-2023

Educational background・Degree

Educational background

-1986 Waseda University Faculty of Science and Engineering
-1991 Waseda University Graduate School, Division of Science and Engineering

Degree

Doctor of Science Waseda University

Master of Business Administration (MBA) Waseda University

Academic Society Joined

The Society of Polymer Science, Japan

The Japan Society of Applied Physics

The Physical Society of Japan

The Chemical Society of Japan

The Crystallographic Society of Japan

The Magnetics Society of Japan

The Ceramic Society of Japan

The Electrochemical Society of Japan

The Pharmaceutical Society of Japan

Research Field

Keywords

Bio-Solid State Science / Chiral Science / Magnetic thin film

Technology Seeds

Light-driven Bending Crystals

Seeds Field:Nanotechnology / Materials

Chemical Health Monitor Kind to Skin

Seeds Field:Life sciences

Paper

Walking and rolling of crystals induced thermally by phase transition

Taniguchi, Takuya; Sugiyama, Haruki; Uekusa, Hidehiro; Shiro, Motoo; Asahi, Toru; Asahi, Toru; Koshima, Hideko

Nature Communications 9(1) 2018/12-2018/12

DOIScopus

Detail

Outline:© 2018 The Author(s). The mechanical motion of materials has been increasingly explored in terms of bending and expansion/contraction. However, the locomotion of materials has been limited. Here, we report walking and rolling locomotion of chiral azobenzene crystals, induced thermally by a reversible single-crystal-to-single-crystal phase transition. Long plate-like crystals with thickness gradient in the longitudinal direction walk slowly, like an inchworm, by repeated bending and straightening under heating and cooling cycles near the transition temperature. Furthermore, thinner, longer plate-like crystals with width gradient roll much faster by tilted bending and then flipping under only one process of heating or cooling. The length of the crystal is shortened above the transition temperature, which induces bending due to the temperature gradient to the thickness direction. The bending motion is necessarily converted to the walking and rolling locomotion due to the unsymmetrical shape of the crystal. This finding of the crystal locomotion can lead to a field of crystal robotics.

The Neuroprotective Effect of Thalidomide against Ischemia through the Cereblon-mediated Repression of AMPK Activity

Sawamura, Naoya; Sawamura, Naoya; Yamada, Mariko; Fujiwara, Miku; Yamada, Haruka; Hayashi, Hideki; Takagi, Norio; Asahi, Toru; Asahi, Toru

Scientific Reports 8(1) 2018/12-2018/12

DOIScopus

Detail

Outline:© 2018 The Author(s). Thalidomide was originally used as a sedative and found to be a teratogen, but now thalidomide and its derivatives are widely used to treat haematologic malignancies. Accumulated evidence suggests that thalidomide suppresses nerve cell death in neurologic model mice. However, detailed molecular mechanisms are unknown. Here we examined the molecular mechanism of thalidomide's neuroprotective effects, focusing on its target protein, cereblon (CRBN), and its binding protein, AMP-activated protein kinase (AMPK), which plays an important role in maintaining intracellular energy homeostasis in the brain. We used a cerebral ischemia rat model of middle cerebral artery occlusion/reperfusion (MCAO/R). Thalidomide treatment significantly decreased the infarct volume and neurological deficits of MCAO/R rats. AMPK was the key signalling protein in this mechanism. Furthermore, we considered that the AMPK-CRBN interaction was altered when neuroprotective action by thalidomide occurred in cells under ischemic conditions. Binding was strong between AMPK and CRBN in normal SH-SY5Y cells, but was weakened by the addition of H 2 O 2 . However, when thalidomide was administered at the same time as H 2 O 2 , the binding of AMPK and CRBN was partly restored. These results suggest that thalidomide inhibits the activity of AMPK via CRBN under oxidative stress and suppresses nerve cell death.

Consolidation of Sm2Fe17N3magnets with Sm-based eutectic alloy binder

Otogawa, Kohei; Takagi, Kenta; Asahi, Toru

Journal of Alloys and Compounds 746p.19 - 262018/05-2018/05

DOIScopus

Detail

ISSN:09258388

Outline:© 2018 Elsevier B.V. Consolidation of Sm 2 Fe 17 N 3 powder with a new Sm-based alloy as a metal binder was examined. Investigation of various Sm-based alloys led to the discovery of a Sm-Fe-Cu-Al alloy having a low melting point of 495 °C, which is more than 100 °C lower than the thermal decomposition temperature of Sm 2 Fe 17 N 3 . The metallographic structure of the Sm-Fe-Cu-Al alloy ingot was a SmCu/Sm eutectic structure containing solid-solution Fe, Cu and Al. Pulverized Sm-Fe-Cu-Al powder as a binder was mixed with Sm 2 Fe 17 N 3 powder, and the mixture was hot-pressed by a current sintering apparatus. The Sm-Fe-Cu-Al alloy binder did not affect the magnetization of Sm 2 Fe 17 N 3 adversely after consolidation. The binder-added Sm 2 Fe 17 N 3 hot-pressed compacts successfully maintained the coercivity of the raw powder over a wide range of hot-pressing temperatures, whereas additive-free Sm 2 Fe 17 N 3 hot-pressed compacts showed a significant decrease in coercivity. Therefore, the Sm-Fe-Cu-Al alloy binder overcame the weakest point of Sm 2 Fe 17 N 3 magnets. XRD analysis suggested that the Sm-Fe-Cu-Al binder restrained precipitation of the α-Fe phase, which is thought to be the probable cause the decrease of coercivity.

A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin

Shitashima, Yoh; Shitashima, Yoh; Shimozawa, Togo; Asahi, Toru; Asahi, Toru; Miyawaki, Atsushi; Miyawaki, Atsushi

Biochemical and Biophysical Research Communications 496(3) p.872 - 8792018/02-2018/02

PubMedDOIScopus

Detail

ISSN:0006291X

Outline:© 2018 Elsevier Inc. UnaG is a green-emitting fluorescent protein that utilizes unconjugated bilirubin (BR) as its fluorophore. While BR has captured the attention of physiologists as an important antioxidant that scavenges reactive oxygen species in biological membranes, its excessive accumulation causes several clinical symptoms. Although the optimal regulation of BR concentration would result in clinical therapies for aging as well as reduce risks of clinical symptoms, UnaG hardly releases BR owing to its extremely high affinity for BR (K d = 98 pM). Herein, we engineered the BR binding and fluorescence of UnaG to be Ca 2+ -sensitive via a genetic insertion of calmodulin (CaM). The resultant UnaG/CaM hybrid protein is a dual-ligand modulable fluorescent protein; binding of the fluorogenic ligand BR is negatively regulated by the other ligand, Ca 2+ ion. The affinity for BR differed by three orders of magnitude between the Ca 2+ -free state (K d = 9.70 nM) and Ca 2+ -saturated state (K d = 9.65 μM). The chimeric protein can release nano- to micro-molar levels of BR with Ca 2+ control, and is thus named BReleaCa (BR + releaser + Ca 2+ ). Such a protein hybridization technique will be generally applicable to change the ligand binding properties of a variety of ligand-inducible functional proteins.

Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG

Shitashima, Yoh; Shitashima, Yoh; Shimozawa, Togo; Kumagai, Akiko; Miyawaki, Atsushi; Asahi, Toru; Asahi, Toru

Biophysical Journal 113(12) p.2805 - 28142017/12-2017/12

PubMedDOIScopus

Detail

ISSN:00063495

Outline:© 2017 Biophysical Society UnaG is a recently discovered ligand-induced fluorescent protein that utilizes bound bilirubin (BR) as its fluorophore. The fluorescence of the UnaG-BR complex (holoUnaG) compares in quantum efficiency to that of enhanced green fluorescent protein, but it is superior in that the fluorophore formation is instantaneous and not dependent on oxygen; hence, much attention has been paid to UnaG as a new fluorescent probe. However, many important molecular properties of fluorescent probes remain unknown, such as the association/dissociation rates of BR, which determine the stability thereof, and the dispersibility of UnaG in aqueous solutions, which influence the functions of labeled proteins. In this study, we found, in the process of investigating the association rate, that the holoUnaG takes two distinct fluorescence states, which we named holoUnaG 1 and holoUnaG 2 . The holoUnaG 1 initially forms after binding BR and then changes to the brighter holoUnaG 2 by a reversible intra-molecular reaction, thereby finally reaching an equilibrium between the two states. Spectroscopic analysis indicated that the intra-molecular reaction was as sociated not with a chemical change of BR but with a change in the environmental conditions surrounding BR. We also revealed that the molecular brightness ratio and equilibrium population ratio of the two states (holoUnaG 1 /holoUnaG 2 ) were 1:3.9 and 6:4, respectively, using photon number counting analysis. From these results, we have suggested a novel schema, to our knowledge, for the formation of the UnaG and BR complex system and have determined the various rate constants associated therein. Additionally, using analytical ultracentrifugation, we established that UnaG in the apo-state (apoUnaG) and the holoUnaG are monomeric in aqueous solution. These findings provide not only key information for the practical use of UnaG as a fluorescent probe, but also the possibility for development of a brighter UnaG mutant by genetic engineering to constitutive holoUnaG 2 .

Optical Activity Anisotropy of Benzil

Nakagawa, Kenta; Martin, Alexander T.; Nichols, Shane M.; Murphy, Veronica L.; Kahr, Bart; Kahr, Bart; Asahi, Toru; Asahi, Toru

Journal of Physical Chemistry C 121(45) p.25494 - 255022017/11-2017/11

DOIScopus

Detail

ISSN:19327447

Outline:© 2017 American Chemical Society. Optical activity (OA) along the optic axis of crystalline benzil has been measured by many over the past 150 years. However, the OA anisotropy remains uncharacterized due to difficulties in sample preparation as well as competition with linear birefringence (LB). The challenges associated with measuring OA along low-symmetry directions in crystals have too often left scientists with only average values of nonresonant OA in solution, i.e., specific rotations, which continue to resist interpretation in terms of structure. Measuring OA anisotropy has been facilitated by recent advances in polarimetry and optical modeling and here we compare results from two distinct division-of-time polarimeters. The absolute structure of crystalline benzil was established for the first time. The optical rotation (OR) of (+)-crystalline benzil (space group P3 1 21) perpendicular to the optic axis at the sodium D-line is -24.6 ± 1.1°/mm. A spectroscopic optical model in the transparent region of the crystal is provided. Electronic structure calculations of OR inform the polarimetric measur ements and point to the necessity of developing linear response theory with periodic boundary conditions in order to interpret the results of chiroptical measurements in crystals.

Block-Copolymer-Assisted Electrochemical Synthesis of Mesoporous Gold Electrodes: Towards a Non-Enzymatic Glucose Sensor

Nugraha, Asep Sugih; Nugraha, Asep Sugih; Li, Cuiling; Bo, Jiang; Bo, Jiang; Iqbal, Muhammad; Iqbal, Muhammad; Alshehri, Saad M.; Ahamad, Tansir; Malgras, Victor; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Asahi, Toru

ChemElectroChem 4(10) p.2571 - 25762017/10-2017/10

DOIScopus

Detail

Outline:© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Sensing technology for biomolecules has played a key role for maintaining a high quality of life. Various kinds of sensing techniques have been developed for detecting biomolecules. Among the detection techniques which have been reported so far, non-enzymatic glucose detection, based on electrochemical mechanisms, has attracted great interest as a facile route for monitoring glucose levels in the blood. In this method, designing nanostructured electrodes is a promising strategy to improve detection performance. Owing to their fascinating physicochemical properties, including superior electrical and electrocatalytic properties, mesoporous metal films offer a wide range of useful applications which cannot be achieved by other mesoporous materials. In this work, mesoporous Au films (MpGFs) are synthesized by an effective and simple electrochemical process in the presence of a surfactant, where the mesoporous structure can be controlled by the size of the polymeric micelles. The micelles are removed using a tetrahydrofuran-based solvent-extraction method. The activity of MpGFs towards glucose oxidation is evaluated under a fixed potential in alkaline solution. Under optimal potentials, the current response for other interfering molecules is minimal, while the current response for glucose is maximal. A linear relationship between the current response and the glucose content is observed over a wide range of concentrations. Thus, our MpGF shows an excellent performance for electrochemical glucose sensing applications.

Fabrication of Mesoporous Cu Films on Cu Foils and Their Applications to Dopamine Sensing

Baba, Daisuke; Baba, Daisuke; Li, Cuiling; Malgras, Victor; Jiang, Bo; Alamri, Hatem R.; Alothman, Zeid A.; Hossain, Md Shahriar A.; Hossain, Md Shahriar A.; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Asahi, Toru

Chemistry - An Asian Journal 12(18) p.2467 - 24702017/09-2017/09

PubMedDOIScopus

Detail

ISSN:18614728

Outline:© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We previously succeeded to prepare stable mesoporous Cu films on Au-coated conductive working electrodes by using polystyrene-b-poly(oxyethylene) (PS 63 000 -b-PEO 26 000 ) micelles as template and sulfuric acid to increase ionic conductivity. In the present study, we report the preparation of mesoporous Cu films on Cu foil. By changing the Cu salts and electrodeposition potentials, we discuss how these parameters influence the final product. Without having to filtrate interefering species, such as uric acid, ascorbic acid and glucose, the dopamine concentration can be precisely determined by applying a suitable potential. Therefore, non-invasive electrochemical sensing based on mesoporous films will be useful for daily diagnosis of mental disorder.

Chiral Sensing with Mesoporous Pd@Pt Nanoparticles

Fang, Yuxi; Fang, Yuxi; Li, Cuiling; Bo, Jiang; Bo, Jiang; Henzie, Joel; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Asahi, Toru

ChemElectroChem 4(8) p.1832 - 18352017/08-2017/08

DOIScopus

Detail

Outline:© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Mesoporous Pd@Pt nanoparticles possess high surface areas while still maintaining electroconductivity and low potential for agglomeration. By introducing L- or D-DOPA (3,4-dihydroxyphenylalanine) enantiomers during the synthesis of the mesoporous Pd@Pt nanoparticles, we can create a Pt shell that is sensitive to the chirality of the DOPA molecule. We demonstrate sensitive electrochemical detection of DOPA enantiomers.

Continuous Mesoporous Pd Films by Electrochemical Deposition in Nonionic Micellar Solution

Iqbal, Muhammad; Iqbal, Muhammad; Li, Cuiling; Wood, Kathleen; Jiang, Bo; Takei, Toshiaki; Dag, Ömer; Dag, Ömer; Baba, Daisuke; Baba, Daisuke; Nugraha, Asep Sugih; Nugraha, Asep Sugih; Asahi, Toru; Whitten, Andrew E.; Hossain, Md Shahriar A.; Malgras, Victor; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke

Chemistry of Materials 29(15) p.6405 - 64132017/08-2017/08

DOIScopus

Detail

ISSN:08974756

Outline:© 2017 American Chemical Society. Mesoporous metals that combine catalytic activity and high surface area can provide more opportunities for electrochemical applications. Various synthetic methods, including hard and soft templating, have been developed to prepare mesoporous/nanoporous metals. Micelle assembly, typically involved in soft-templates, is flexible and convenient for such purposes. It is, however, difficult to control, and the ordering is significantly destroyed during the metal deposition process, which is detrimental when it comes to designing precisely mesostructured materials. In the present work, mesoporous Pd films were uniformly electrodeposited using a non ionic surfactant, triblock copolymer poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), as a pore-directing agent. The interaction between micelles and metal precursors greatly influences the metal growth and determines the final structure. The water-coordinated species interact with the ethylene oxide moiety of the micelles to effectively drive the Pd(II) species toward the working electrode surface. From small-angle neutron scattering data, it is found that spherical P123 micelles, with an average diameter of ∼14 nm, are formed in the electrolyte, and the addition of Pd ions does not significantly modify their structure, which is the essence of the micelle assembly approach. The uniformly sized mesopores are formed over the entire mesoporous Pd film and have an average pore diameter of 10.9 nm. Cross-sectional observation of the film also shows mesopores spanning continuously from the bottom to the top of the film. The crystallinity, crystal phase, and electronic coordination state of the Pd film are also confirmed. Through this study, it is found that the optimized surfactant concentration and applied deposition potential are the key factors to govern the formation of homogeneous and well-distributed pores over the entire film. Interestingly, the as-prepared mesoporous Pd films exhibit superior electrocatalytic activity toward the ethanol oxidation reaction by fully utilizing the accessible active surface area. Our approach combines electrochemistry with colloidal and coordination chemistry and is widely applicable to other promising metals and alloy electrocatalysts.

LC–MS/MS and chiroptical spectroscopic analyses of multidimensional metabolic systems of chiral thalidomide and its derivatives

Ogino, Yoshiyuki; Tanaka, Masahito; Shimozawa, Togo; Asahi, Toru

Chirality 29(6) p.282 - 2932017/06-2017/06

DOIScopus

Detail

ISSN:08990042

Outline:© 2017 Wiley Periodicals, Inc. Enantiomeric thalidomide undergoes various kinds of biotransformations including chiral inversion, hydrolysis, and enzymatic oxidation, which results in several metabolites, thereby adding to the complexity in the understanding of the nature of thalidomide. To decipher this complexity, we analyzed the multidimensional metabolic reaction networks of thalidomide and related molecules in vitro. Characteristic patterns in the amount of various metabolites of thalidomide and related molecules generated during a combination of chiral inversion, hydrolysis, and hydroxylation were observed using liquid chromatography–tandem mass spectrometry and chiroptical spectroscopy. We found that monosubstituted thalidomide derivatives exhibited different time-dependent metabolic patterns compared with thalidomide. We also revealed that monohydrolyzed and monohydroxylated metabolites of thalidomide were likely to generate mainly by a C-5 oxidation of thalidomide and subsequent ring opening of the hydroxylated metabolite. Since chirality was conserved in most of these metabolites during metabolism, they had the same chirality as that of nonmetabolized thalidomide. Our findings will contribute toward understanding the significant pharmacological effects of the multiple metabolites of thalidomide and its derivatives.

Accurate measurement of the optical activity of alanine crystals and the determination of their absolute chirality

Ishikawa, Kazuhiko; Terasawa, Yukana; Tanaka, Masahito; Asahi, Toru; Asahi, Toru

Journal of Physics and Chemistry of Solids 104p.257 - 2662017/05-2017/05

DOIScopus

Detail

ISSN:00223697

Outline:© 2017 Elsevier Ltd Wavelength dependence measurements of the chiroptical properties in alanine crystals have so far been unsuccessful using conventional spectroscopic techniques. We describe our attempts to measure the wavelength dependence of the optical activity in L- and D-alanine crystals along each crystallographic axis, and to determine the absolute chirality of alanine crystals by correlating the absolute structure to the optical activity using an x-ray diffractometer and a generalized high accuracy universal polarimeter. We have succeeded in accurately measuring the optical rotatory dispersion in the < 010 > direction, which shows that the optical rotation of the D-alanine crystal is dextrorotatory and that of the L-alanine crystal is laevorotatory, thereby determining the absolute chirality. Furthermore, comparison with the optical activity in solution shows that the optical activity in alanine crystals is different not only in value, but also in the sign. These results have led us to conclude that the optical rotatory power in the crystalline state should not be simply the summation of molecular optical rotatory power values. We propose the necessity of a theory, which contains the contribution of molecular interactions within the crystal, in order to calculate the optical rotatory power of the crystalline state.

Fast-type high-accuracy universal polarimeter using charge-coupled device spectrometer

Takanabe, Akifumi; Koshima, Hideko; Asahi, Toru; Asahi, Toru

AIP Advances 7(2) 2017/02-2017/02

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Detail

Outline:© 2017 Author(s). A fast, high-accuracy universal polarimeter was developed using a charge-coupled device (CCD) spectrometer (CCD-HAUP), to carry out simultaneous optical anisotropic (linear birefringence, LB; linear dichroism, LD) and chiroptical (circular birefringence, CB; circular dichroism, CD) measurements on single crystals without any pretreatment, in the visible region between 400-680 nm. The principle of the HAUP method is to measure the intensities of emergent light passing through a polarizer, a crystal sample, and then an analyzer, as the azimuth angles of the polarizer and analyzer are independently altered. The CCD-HAUP has the unique feature that white transmitted light intensity can be measured using a CCD spectrometer, compared with the generalized HAUP (G-HAUP) system in which monochromatic transmitted light is measured using a photomultiplier. The CCD-HAUP measurements across the entire wavelength region are completed within the G-HAUP measurement time for a single wavelength. The CCD-HAUP drastically reduces the measurement time for a dataset to only 1.5 h, from the 24 h required for the G-HAUP system. LB, LD, CB, and CD measurements of single crystals of α-quartz and enantiomeric photomechanical salicylidenephenylethylamines before, during, and after ultraviolet light irradiation show results comparable to those obtained using the G-HAUP system. The newly developed system is very effective for samples susceptible to degradation induced by external stimuli, such as light and heat.

Reversible single-crystal-to-single-crystal phase transition of chiral salicylidenephenylethylamine

Takanabe, Akifumi; Katsufuji, Takuro; Johmoto, Kohei; Uekusa, Hidehiro; Shiro, Motoo; Koshima, Hideko; Asahi, Toru; Asahi, Toru

Crystals 7(1) 2017/01-2017/01

DOIScopus

Detail

Outline:© 2016 by the author; licensee MDPI, Basel, Switzerland.The chiral crystal of enantiomeric (S)-N-3,5-di-tert-butylsalicylidene-1-phenylethylamine in the enol form [enol-(S)-1] undergoes a reversible single-crystal-to-single-crystal (SCSC) phase transition at Tc ≈ 3 °C from the room temperature α-form in orthorhombic space group P212121 (Zʹ = 1) to the low temperature β-form in the monoclinic space group P21 (Zʹ = 2) with a thermal hysteresis of approximately 1.7 °C. A detailed comparison of the crystal structures of the α-and β-forms revealed that the 5-tert-butyl group of one molecule in the asymmetric unit of the β-form rotated by ca. 60°, and the dihedral angle between the phenyl and salicyl planes increased slightly in the β-form crystal. However, the changes in the molecular conformation and packing arrangement are small, which leads to the reversible SCSC phase transition with no destruction of the crystal lattice. The dielectric constant along the b-axis was small, probably due to the weak intermolecular interactions in the crystals.

Solid-state photochemical reaction of multisubstituted thymine derivatives

Udagawa, Akihiro; Johnston, Priscilla; Uekusa, Hidehiro; Koshima, Hideko; Saito, Kei; Asahi, Toru; Asahi, Toru

ACS Sustainable Chemistry and Engineering 4(11) p.6107 - 61142016/11-2016/11

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Detail

Outline:© 2016 American Chemical Society.Solid-state photochemical reactions in crystals, known as topochemical reactions, are solvent-free green chemical reactions that produce stereospecific molecules. The photoreaction of thymine is interesting because the dimeric photoproduct can form four types of stereoisomers and when the dimer is formed in DNA helices it can cause skin cancers. We investigated the photoreaction of five multisubstituted thymine derivatives in the solid-state, which were designed with crystal engineering concepts to promote π-π stacking of benzene rings in the crystal. Powder X-ray diffraction analysis revealed that a para-substituted bis-thymine derivative was aligned along the c axis in the crystal and was susceptible to topochemical reaction to form a polymer, as previously reported. Ortho- and meta-substituted bis-thymine derivatives and a tetrakis-substituted derivative were found to be topochemically unreactive using both gel permeation chromatography (GPC) and X-ray crystal structural analysis. The tris-substituted thymine derivative was found to be topochemically reactive due to favorable crystal packing, which included ethanol molecules to form hydrogen bonding with one of the thymine moieties and stabilize the crystal packing. GPC and crystal structural analysis revealed that it could form tetramer at most via topochemical [2+2]-cycloaddition upon UV irradiation. Based on the crystal structure of the tris-substituted thymine derivative, the structure of the tetrameric photoproduct is expected to link via cis-syn, trans-anti and cis-syn cyclobutane isomers.

Optical Activity and Optical Anisotropy in Photomechanical Crystals of Chiral Salicylidenephenylethylamines

Takanabe, Akifumi; Tanaka, Masahito; Johmoto, Kohei; Uekusa, Hidehiro; Mori, Tadashi; Koshima, Hideko; Asahi, Toru; Asahi, Toru

Journal of the American Chemical Society 138(45) p.15066 - 150772016/11-2016/11

DOIScopus

Detail

ISSN:00027863

Outline:© 2016 American Chemical Society.Introducing chirality into photomechanical crystals is beneficial for the diversification of mechanical motion. Measurement of the chiroptical and optical anisotropic properties of chiral crystals is indispensable for evaluating photomechanical crystals. The platelike crystals of S- and R-enantiomers of photochromic N-3,5-di-tert-butylsalicylidene-1-phenylethylamine in enol form (enol-(S)-1 and enol-(R)-1) caused bending motion with twisting upon ultraviolet (UV) light irradiation, due to shrinkage along the length and width directions of the irradiated surface, based on the optimized crystal structure of the photoisomerized trans-keto-(S)-1. By employing the generalized high-accuracy universal polarimeter (G-HAUP), optical anisotropic (linear birefringence, LB; linear dichroism, LD) as well as chiroptical (circular birefringence, CB; circular dichroism, CD) spectra of both the enantiomeric crystals on the (001) face were simultaneously measured before and under continuous UV irradiation. The LD peak was observed at 330 nm in the negative sign, derived from the π-π∗ transition of the intramolecularly hydrogen-bonded salicylidenimino moiety. The CD spectra of the S and R crystals revealed the negative and positive Cotton effect at 330 nm, respectively, and new peaks appeared at 460 nm under UV light irradiation due to photoisomerization to the S and R trans-keto isomers at around 10% conversion. The CB and CD spectra evaluated by the HAUP measurement were opposite to those measured in the hexane solution, as well as those simulated by quantum chemical calculation. The dissymmetry parameter, g, of the enol-(S)-1 crystal along the c axis (0.013) was approximately 10 times larger than the g values in the solution (0.0010) and by calculation (0.0016).

Ohgata, the single Drosophila ortholog of human cereblon, regulates insulin signaling-dependent organismic growth

Wakabayashi, Satoru; Sawamura, Naoya; Sawamura, Naoya; Voelzmann, André; Broemer, Meike; Asahi, Toru; Asahi, Toru; Hoch, Michael

Journal of Biological Chemistry 291(48) p.25120 - 251322016/11-2016/11

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ISSN:00219258

Outline:© 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Cereblon (CRBN) is a substrate receptor of the E3 ubiquitin ligase complex that is highly conserved in animals and plants. CRBN proteins have been implicated in various biological processes such as development, metabolism, learning, and memory formation, and their impairment has been linked to autosomal recessive non-syndromic intellectual disability and cancer. Furthermore, human CRBN was identified as the primary target of thalidomide teratogenicity. Data on functional analysis of CRBN family members in vivo, however, are still scarce. Here we identify Ohgata (OHGT), the Drosophila ortholog of CRBN, as a regulator of insulin signaling-mediated growth. Using ohgt mutants that we generated by targeted mutagenesis, we show that its loss results in increased body weight and organ size without changes of the body proportions. We demonstrate that ohgt knockdown in the fat body, an organ analogous to mammalian liver and adipose tissue, phenocopies the growth phenotypes. We further show that overgrowth is due to an elevation of insulin signaling in ohgt mutants and to the down-regulation of inhibitory cofactors of circulating Drosophila insulin-like peptides (DILPs), named acid-labile subunit and imaginal morphogenesis protein-late 2. The two inhibitory proteins were previously shown to be components of a heterotrimeric complex with growth-promoting DILP2 and DILP5. Our study reveals OHGT as a novel regulator of insulin-dependent organismic growth in Drosophila.

Nuclear cereblon modulates transcriptional activity of Ikaros and regulates its downstream target, enkephalin, in human neuroblastoma cells

Wada, Takeyoshi; Asahi, Toru; Asahi, Toru; Sawamura, Naoya; Sawamura, Naoya

Biochemical and Biophysical Research Communications 477(3) p.388 - 3942016/08-2016/08

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ISSN:0006291X

Outline:© 2016 Elsevier Inc. The gene coding cereblon (CRBN) was originally identified in genetic linkage analysis of mild autosomal recessive nonsyndromic intellectual disability. CRBN has broad localization in both the cytoplasm and nucleus. However, the significance of nuclear CRBN remains unknown. In the present study, we aimed to elucidate the role of CRBN in the nucleus. First, we generated a series of CRBN deletion mutants and determined the regions responsible for the nuclear localization. Only CRBN protein lacking the N-terminal region was localized outside of the nucleus, suggesting that the N-terminal region is important for its nuclear localization. CRBN was also identified as a thalidomide-binding protein and component of the cullin-4-containing E3 ubiquitin ligase complex. Thalidomide has been reported to be involved in the regulation of the transcription factor Ikaros by CRBN-mediated degradation. To investigate the nuclear functions of CRBN, we performed co-immunoprecipitation experiments and evaluated the binding of CRBN to Ikaros. As a result, we found that CRBN was associated with Ikaros protein, and the N-terminal region of CRBN was required for Ikaros binding. In luciferase reporter gene experiments, CRBN modulated transcriptional activity of Ikaros. Furthermore, we found that CRBN modulated Ikaros-mediated transcriptional repression of the proenkephalin gene by binding to its promoter region. These results suggest that CRBN binds to Ikaros via its N-terminal region and regulates transcriptional activities of Ikaros and its downstream target, enkephalin.

Mitochondrial cereblon functions as a Lon-type protease

Kataoka, Kosuke; Nakamura, China; Asahi, Toru; Asahi, Toru; Sawamura, Naoya; Sawamura, Naoya

Scientific Reports 62016/07-2016/07

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Outline:Lon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix, and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. However, whether CRBN has Lon protease-like function remains unknown. Here, we determined if CRBN has a protective function against oxidative stress, similar to Lon protease. We report that CRBN partially distributes in mitochondria, suggesting it has a mitochondrial function. To specify the mitochondrial role of CRBN, we mitochondrially expressed CRBN in human neuroblastoma SH-SY5Y cells. The resulting stable SH-SY5Y cell line showed no apparent effect on the mitochondrial functions of fusion, fission, and membrane potential. However, mitochondrially expressed CRBN exhibited protease activity, and was induced by oxidative stress. In addition, stably expressed cells exhibited suppressed neuronal cell death induced by hydrogen peroxide. These results suggest that CRBN functions specifically as a Lon-type protease in mitochondria.

Mechanical Motion of Chiral Azobenzene Crystals with Twisting upon Photoirradiation

Taniguchi, Takuya; Fujisawa, Juri; Shiro, Motoo; Koshima, Hideko; Asahi, Toru; Asahi, Toru

Chemistry - A European Journal 22(23) p.7950 - 79582016/06-2016/06

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ISSN:09476539

Outline:© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.The photomechanical motion of chiral crystals of trans-azobenzene derivatives with an (S)- and (R)-phenylethylamide group was investigated and compared with a racemic crystal. Changes in the UV/Vis absorption spectra of the powdered crystals before and after UV irradiation were measured by using an optical waveguide spectrometer, showing that the lifetime of the cis-to-trans thermal back-isomerization of the chiral crystals was faster than that of the racemic crystals. Upon UV irradiation, a long plate-like chiral microcrystal bent away from the light source with a twisting motion. A square-like chiral microcrystal curled toward the light with some twisting. Reversible bending of a rod-like chiral microcrystal was repeatable over twenty-five cycles. In contrast, bending of a plate-like racemic microcrystal was small. A possible mechanism for the bending and twisting motion was discussed based on the optimized cis conformer determined by using calculations, showing that the bending motion with twisting is caused by elongation along the b axis and shrinkage along the a axis. Twist and bent: A plate-like chiral azobenzene crystal bent backward with twisting upon UV irradiation from the front. UV irradiation from the rear bent forward the crystal with twisting (see figure).

Interfacial effects on the crystallization and surface properties of poly(l-lactic acid) ultrathin films

Udagawa, Akihiro; Fujie, Toshinori; Kawamoto, Yuko; Saito, Akihiro; Takeoka, Shinji; Asahi, Toru; Asahi, Toru

Polymer Journal 48(2) p.157 - 1612016/02-2016/02

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ISSN:00323896

Outline:© 2016 The Society of Polymer Science, Japan (SPSJ).Polymer crystallization affects structural and mechanical properties of polymer thin films. In this study, we focused on the thermal annealing-induced crystallization in semi-crystalline poly(l-lactic acid) (PLLA) ultrathin films (referred as nanosheets) was investigated in terms of interfacial interaction of PLLA with air and substrate. The surface structure of the PLLA nanosheets observed by atomic force microscopy showed that roughness of the air-side surface increased due to crystallization of PLLA under thermal annealing, whereas that of the substrate-side surface changed little. The elastic moduli and the physical adhesiveness of the nanosheets also changed only on the surface of the air side from crystallization, in contrast to the substrate side. The X-ray diffraction studies of the PLLA nanosheets with different thickness showed that the crystalline contents steeply increased below ca. 200 nm. These results indicated that the crystallization was enhanced near the surface of the air side and restricted near that of the substrate side due to the different interfacial association of the polymer chains in the nanosheet.

Absolute structure determination of chiral crystals consisting of achiral benzophenone with single-crystal X-ray Diffraction and its correlation with solid-state circular dichroism

Matsumoto, Arimasa; Tsuchiya, Sumeru; Hagiwara, Yuki; Ishikawa, Kazuhiko; Koshima, Hideko; Asahi, Toru; Asahi, Toru; Soai, Kenso

Chemistry Letters 45(5) p.526 - 5282016/01-2016/01

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ISSN:03667022

Outline:© 2016 The Chemical Society of Japan.Achiral benzophenone is one of the simplest organic aromatic ketones that crystallize in the chiral form. The absolute structure of chiral crystals consisting of achiral benzophenone was firstly determined with single-crystal X-ray diffraction analysis and the absolute structure was successfully correlated with the solid-state circular dichroism spectra.

Mechanical motion of molecular crystals induced by [4 + 4] photodimerisation

Koshima, Hideko; Uchimoto, Hidetaka; Taniguchi, Takuya; Nakamura, Jun; Asahi, Tsuyoshi; Asahi, Toru; Asahi, Toru

CrystEngComm 18(38) p.7305 - 73102016/01-2016/01

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Outline:© 2016 The Royal Society of Chemistry.The photomechanical bending of crystals of a stilbene-type compound substituted with anthracene and indanone groups, (E)-2-(9-anthrylmethylene)-1-indanone (trans-1), was investigated. When a narrow plate-like microcrystal was irradiated with ultraviolet (UV) light at 365 nm, the crystal gradually bent away from the light source and finally reached a semicircular shape after more than 10 min. A larger rod-like crystal, approximately 10 mm in length, also exhibited a slight bending motion. The cessation of UV irradiation caused the bent crystals to return very slowly to their straight form. In the crystals, the anthracene planes of two neighbouring trans-1 molecules are arranged in a head-to-tail parallel manner, with a short plane-to-plane distance of only 3.72 Å. The 1H nuclear magnetic resonance spectra of trans-1 crystals before and after UV irradiation revealed the intermolecular [4 + 4] photodimerisation of the two anthracene planes, while the trans-to-cis photoisomerisation was not significant. The UV-vis absorption spectra of the trans-1 powder crystals, obtained using a diffuse reflectance spectrophotometer, showed a gradual increase in absorbance between 200 and 500 nm with increasing UV irradiation time, reaching a maximum after 1 h. Thermal back-monomerisation was very slow in the dark, not recovering the initial spectrum even after 25 days. The fluorescence spectra at 570 nm, derived from the anthracene excimer, decreased in intensity with increasing UV irradiation time due to a decrease in the amount of anthracene chromophore via photodimerisation. In situ X-ray measurements revealed that the bending of the crystals was caused by slight elongation of the b axis of the unit cell, corresponding to the long axis of the rod-like crystals. Calculations revealed that the observed crystal elongation could be explained by an optimised head-to-tail [4 + 4] orientation of the anthracene dimer.

Optical Activity and Optical Anisotropy in Photomechanical Crystals of Chiral Salicylidenephenylethylamines

Takanabe, Akifumi;Tanaka, Masahito;Johmoto, Kohei;Uekusa, Hidehiro;Mori, Tadashi;Koshima, Hideko;Asahi, Toru

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138(45) p.15066 - 150772016-2016

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ISSN:0002-7863

Ohgata, the Single Drosophila Ortholog of Human Cereblon, Regulates Insulin Signaling-dependent Organismic Growth

Wakabayashi, Satoru;Sawamura, Naoya;Voelzmann, Andre;Broemer, Meike;Asahi, Toru;Hoch, Michael

JOURNAL OF BIOLOGICAL CHEMISTRY 291(48) p.25120 - 251322016-2016

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ISSN:0021-9258

Microwave Effect on Fischer Esterification

Koshima Hideko;Miyazaki Kiminori;Ishii Saori;Asahi Toru

Chemistry Letters 0(0) 2016-2016

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ISSN:0366-7022

Outline:Upon microwave irradiation, esterification of octanoic acid with 1-octanol in the presence of hydrochloric acid as an acid catalyst proceeded more efficiently in glass vessels than in silicon carbide (SiC) vessels, affording 1-octyl octanoate at higher yields and revealing microwave effects during esterification.

Absolute Structure Determination of Chiral Crystals Consisting of Achiral Benzophenone with Single-crystal X-ray Diffraction and Its Correlation with Solid-state Circular Dichroism

Matsumoto Arimasa;Tsuchiya Sumeru;Hagiwara Yuki;Ishikawa Kazuhiko;Koshima Hideko;Asahi Toru;Soai Kenso

Chemistry Letters 45(5) p.526 - 5282016-2016

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Detail

ISSN:0366-7022

Outline:Achiral benzophenone is one of the simplest organic aromatic ketones that crystallize in the chiral form. The absolute structure of chiral crystals consisting of achiral benzophenone was firstly determined with single-crystal X-ray diffraction analysis and the absolute structure was successfully correlated with the solid-state circular dichroism spectra.

Microwave Effect on Fischer Esterification

Koshima Hideko;Miyazaki Kiminori;Ishii Saori;Asahi Toru

Chemistry Letters 45(5) p.505 - 5072016-2016

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ISSN:0366-7022

Outline:Upon microwave irradiation, esterification of octanoic acid with 1-octanol in the presence of hydrochloric acid as an acid catalyst proceeded more efficiently in glass vessels than in silicon carbide (SiC) vessels, affording 1-octyl octanoate in higher yields and revealing microwave effects during esterification.

18pPSA-61 Magneto-optical study on Cerium fluoride crystal with the G-HAUP

Nakagawa K.;Villora G.;Shimamura K.;Asahi T.

70(2) 2015/09-2015/09

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ISSN:2189079X

19aPS-14 Optical property and symmetry breaking of cupper-oxide based high-temperature superconducting material Bi_2Sr_2CaCu_2O_<8+x>

Matsumoto M.;Nakagawa K.;Watauchi S;Matsuda A.;Asahi T.

70(2) 2015/09-2015/09

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ISSN:2189079X

Cereblon is recruited to aggresome and shows cytoprotective effect against ubiquitin-proteasome system dysfunction

Sawamura, Naoya; Sawamura, Naoya; Wakabayashi, Satoru; Matsumoto, Kodai; Yamada, Haruka; Asahi, Toru; Asahi, Toru

Biochemical and Biophysical Research Communications 464(4) p.1054 - 10592015/09-2015/09

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ISSN:0006291X

Outline:© 2015 Elsevier Inc. All rights reserved. Cereblon (CRBN) is encoded by a candidate gene for autosomal recessive nonsyndromic intellectual disability (ID). The nonsense mutation, R419X, causes deletion of 24 amino acids at the C-terminus of CRBN, leading to mild ID. Although abnormal CRBN function may be associated with ID disease onset, its cellular mechanism is still unclear. Here, we examine the role of CRBN in aggresome formation and cytoprotection. In the presence of a proteasome inhibitor, exogenous CRBN formed perinuclear inclusions and co-localized with aggresome markers. Endogenous CRBN also formed perinuclear inclusions under the same condition. Treatment with a microtubule destabilizer or an inhibitor of the E3 ubiquitin ligase activity of CRBN blocked formation of CRBN inclusions. Biochemical analysis showed CRBN containing inclusions were high-molecular weight, ubiquitin-positive. CRBN overexpression in cultured cells suppressed cell death induced by proteasome inhibitor. Furthermore, knockdown of endogenous CRBN in cultured cells increased cell death induced by proteasome inhibitor, compared with control cells. Our results show CRBN is recruited to aggresome and has functional roles in cytoprotection against ubiquitin-proteasome system impaired condition.

Model of complex chiral drug metabolic systems and numerical simulation of the remaining chirality toward analysis of dynamical pharmacological activity

Ogino, Yoshiyuki; Asahi, Toru

Journal of Theoretical Biology 373p.117 - 1312015/05-2015/05

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Detail

ISSN:00225193

Outline:© 2015 Elsevier Ltd. In this study, systems of complicated pathways involved in chiral drug metabolism were investigated. The development of chiral drugs resulted in significant improvement in the remedies available for the treatment of various severe sicknesses. Enantiopure drugs undergo various biological transformations that involve chiral inversion and thus result in the generation of multiple enantiomeric metabolites. Identification of the specific active substances determining a given drug[U+05F3]s efficacy among such a mixture of different metabolites remains a challenge. To comprehend this complexity, we constructed a mathematical model representing the complicated metabolic pathways simultaneously involving chiral inversion. Moreover, this model is applied to the metabolism of thalidomide, which has recently been revived as a potentially effective prescription drug for a number of intractable diseases. The numerical simulation results indicate that retained chirality in the metabolites reflects the original chirality of the unmetabolized drug, and a higher level of enantiomeric purity is preserved during spontaneous degradation. In addition, chirality remaining after equilibration is directly related to the rate constant not only for chiral inversion but also for generation and degradation. Furthermore, the retention of chirality is quantitatively predictable using this combination of kinetic parameters. Our simulation results well explain the behavior of thalidomide in the practical biological experimental data. Therefore, this model promises a comprehensive understanding of dynamic metabolic systems involving chiral drugs that express multiple enantiospecific drug efficacies.

Self-disproportionation of enantiomers of thalidomide and its fluorinated analogue via gravity-driven achiral chromatography: mechanistic rationale and implications

Maeno, Mayaka;Tokunaga, Etsuko;Yamamoto, Takeshi;Suzuki, Toshiya;Ogino, Yoshiyuki;Ito, Emi;Shiro, Motoo;Asahi, Toru;Shibata, Norio

CHEMICAL SCIENCE 6(2) p.1043 - 10482015-2015

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ISSN:2041-6520

Model of complex chiral drug metabolic systems and numerical simulation of the remaining chirality toward analysis of dynamical pharmacological activity

Ogino, Yoshiyuki;Asahi, Toru

JOURNAL OF THEORETICAL BIOLOGY 373p.117 - 1312015-2015

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ISSN:0022-5193

Cereblon is recruited to aggresome and shows cytoprotective effect against ubiquitin-proteasome system dysfunction

Sawamura, Naoya;Wakabayashi, Satoru;Matsumoto, Kodai;Yamada, Haruka;Asahi, Toru

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 464(4) p.1054 - 10592015-2015

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ISSN:0006-291X

Crystal structure of (S)-4-carbamoyl-4-(1,3-dioxo-isoindolin-2-yl)butanoic acid.

Otogawa Kohei;Ishikawa Kazuhiko;Shiro Motoo;Asahi Toru

Acta crystallographica. Section E, Crystallographic communications 71(Pt 1) 2015-2015

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Outline::In the title compound, C13H12N2O5, the phthalimide ring system is essentially planar, with a maximum deviation of 0.0479 (14) Å. In the crystal, each mol-ecule is linked via six neighbouring mol-ecules into a three-dimensional network through N-H⋯O and O-H⋯O hydrogen bonds, which form an R 3 (2)(8) ring motif.

Model of complex chiral drug metabolic systems and numerical simulation of the remaining chirality toward analysis of dynamical pharmacological activity.

Ogino Yoshiyuki;Asahi Toru

Journal of theoretical biology 3732015-2015

PubMedDOI

Detail

ISSN:1095-8541

Outline::In this study, systems of complicated pathways involved in chiral drug metabolism were investigated. The development of chiral drugs resulted in significant improvement in the remedies available for the treatment of various severe sicknesses. Enantiopure drugs undergo various biological transformations that involve chiral inversion and thus result in the generation of multiple enantiomeric metabolites. Identification of the specific active substances determining a given drug׳s efficacy among such a mixture of different metabolites remains a challenge. To comprehend this complexity, we constructed a mathematical model representing the complicated metabolic pathways simultaneously involving chiral inversion. Moreover, this model is applied to the metabolism of thalidomide, which has recently been revived as a potentially effective prescription drug for a number of intractable diseases. The numerical simulation results indicate that retained chirality in the metabolites reflects the original chirality of the unmetabolized drug, and a higher level of enantiomeric purity is preserved during spontaneous degradation. In addition, chirality remaining after equilibration is directly related to the rate constant not only for chiral inversion but also for generation and degradation. Furthermore, the retention of chirality is quantitatively predictable using this combination of kinetic parameters. Our simulation results well explain the behavior of thalidomide in the practical biological experimental data. Therefore, this model promises a comprehensive understanding of dynamic metabolic systems involving chiral drugs that express multiple enantiospecific drug efficacies.

Cereblon is recruited to aggresome and shows cytoprotective effect against ubiquitin-proteasome system dysfunction.

Sawamura Naoya;Wakabayashi Satoru;Matsumoto Kodai;Yamada Haruka;Asahi Toru

Biochemical and biophysical research communications 464(4) 2015-2015

PubMedDOI

Detail

ISSN:1090-2104

Outline::Cereblon (CRBN) is encoded by a candidate gene for autosomal recessive nonsyndromic intellectual disability (ID). The nonsense mutation, R419X, causes deletion of 24 amino acids at the C-terminus of CRBN, leading to mild ID. Although abnormal CRBN function may be associated with ID disease onset, its cellular mechanism is still unclear. Here, we examine the role of CRBN in aggresome formation and cytoprotection. In the presence of a proteasome inhibitor, exogenous CRBN formed perinuclear inclusions and co-localized with aggresome markers. Endogenous CRBN also formed perinuclear inclusions under the same condition. Treatment with a microtubule destabilizer or an inhibitor of the E3 ubiquitin ligase activity of CRBN blocked formation of CRBN inclusions. Biochemical analysis showed CRBN containing inclusions were high-molecular weight, ubiquitin-positive. CRBN overexpression in cultured cells suppressed cell death induced by proteasome inhibitor. Furthermore, knockdown of endogenous CRBN in cultured cells increased cell death induced by proteasome inhibitor, compared with control cells. Our results show CRBN is recruited to aggresome and has functional roles in cytoprotection against ubiquitin-proteasome system impaired condition.

Arctic mutatn Aβ40 aggregates on α7 nicotinic acetylcholine receptors and inhibits their functions

Ye Ju, Toru Asahi and Naoya Sawamura

Journal of Neurochemistry 2014/08-

Quantitative analysis with advanced compensated polarized light microscopy on wavelength dependence of linear birefringence of single crystals causing arthritis

Akifumi Takanabe, Masahito Tanaka, Atsuo Taniguchi, Hisashi Yamanaka and Toru Asahi

Journal of Physics D: Applied Physics 47(28) p.2854022014/06-

A label-free electrical assay of fibrous amyloid β based on semiconductor biosensing

Sho Hideshima, Masumi Kobayashi, Takeyoshi Wada, Shigeki Kuroiwa, Takuya Nakanishi, Naoya Sawamura, Toru Asahi and Tetsuya Osaka

chemical communications 50p.3476 - 34792014/02-

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes

Nakagawa, Kenta;Harper-Lovelady, Heather;Tanaka, Yuji;Tanaka, Masahito;Yamato, Masayuki;Asahi, Toru

CHEMICAL COMMUNICATIONS 50(95) p.15086 - 150892014-2014

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ISSN:1359-7345

A label-free electrical assay of fibrous amyloid beta based on semiconductor biosensing

Hideshima, Sho;Kobayashi, Masumi;Wada, Takeyoshi;Kuroiwa, Shigeki;Nakanishi, Takuya;Sawamura, Naoya;Asahi, Toru;Osaka, Tetsuya

CHEMICAL COMMUNICATIONS 50(26) p.3476 - 34792014-2014

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ISSN:1359-7345

Quantitative analysis with advanced compensated polarized light microscopy on wavelength dependence of linear birefringence of single crystals causing arthritis

Takanabe, Akifumi;Tanaka, Masahito;Taniguchi, Atsuo;Yamanaka, Hisashi;Asahi, Toru

JOURNAL OF PHYSICS D-APPLIED PHYSICS 47(28) 2014-2014

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ISSN:0022-3727

Arctic mutant A beta 40 aggregates on alpha 7 nicotinic acetylcholine receptors and inhibits their functions

Ju, Ye;Asahi, Toru;Sawamura, Naoya

JOURNAL OF NEUROCHEMISTRY 131(5) p.667 - 6742014-2014

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ISSN:0022-3042

A label-free electrical assay of fibrous amyloid β based on semiconductor biosensing.

Hideshima Sho;Kobayashi Masumi;Wada Takeyoshi;Kuroiwa Shigeki;Nakanishi Takuya;Sawamura Naoya;Asahi Toru;Osaka Tetsuya

Chemical communications (Cambridge, England) 50(26) 2014-2014

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ISSN:1364-548X

Outline::We propose, as an alternative to conventional spectroscopic assays, a simple method for discriminating fibrous amyloid proteins by using a label-free semiconductor-based biosensor. The highly sensitive assay is expected to be useful for accelerating amyloid related research.

Arctic mutant Aβ40 aggregates on α7 nicotinic acetylcholine receptors and inhibits their functions.

Ju Ye;Asahi Toru;Sawamura Naoya

Journal of neurochemistry 131(5) 2014-2014

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ISSN:1471-4159

Outline::Amyloid β protein (Aβ) plays a central role in the pathogenesis of Alzheimer's disease (AD). Point mutations within the Aβ sequence associated with familial AD (FAD) are clustered around the central hydrophobic core of Aβ. Several types of mutations within the Aβ sequence have been identified, and the 'Arctic' mutation (E22G) has a purely cognitive phenotype typical of AD. Previous studies have shown that the primary result of the 'Arctic' mutation is increased formation of Aβ protofibrils. However, the molecular mechanism underlying this effect remains unknown. Aβ42 binds to a neuronal nicotinic acetylcholine receptor subunit, neuronal acetylcholine receptor subunit alpha-7 (CHRNA7), with high affinity and, thus, may be involved in the pathogenesis of AD. Therefore, to clarify the molecular mechanism of Arctic mutation-mediated FAD, we focused on CHRNA7 as a target molecule of Arctic Aβ. We performed an in vitro binding assay using purified CHRNA7 and synthetic Arctic Aβ40, and demonstrated that Arctic Aβ40 specifically bound to CHRNA7. The aggregation of Arctic Aβ40 was enhanced with the addition of CHRNA7. Furthermore, the function of CHRNA7 was detected by measuring Ca(2+) flux and phospho-p44/42 MAPK (ERK1/2) activation. Our results indicated that Arctic Aβ40 aggregation was enhanced by the addition of CHRNA7, which destabilized the function of CHRNA7 via inhibition of Ca(2+) responses and activation of ERK1/2. These findings indicate that Arctic Aβ mutation may be involved in the mechanism underlying FAD. This mechanism may involve binding and aggregation, leading to the inhibition of CHRNA7 functions.

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes.

Nakagawa Kenta;Harper-Lovelady Heather;Tanaka Yuji;Tanaka Masahito;Yamato Masayuki;Asahi Toru

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes. 50(95) 2014-2014

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ISSN:1364-548X

Outline::A novel method for fabricating anisotropic collagen membranes, which regulate the preferred orientation, is developed. The high-accuracy universal polarimeter (HAUP) method is used for simultaneous and quantitative evaluations of the optical anisotropy and optical activity. The evaluation of these optical quantities, enabled by the HAUP method, is beneficial to the field of tissue engineering.

Carrier Design and Graduate School Reform for Postadoctoral Fellows and Doctoral Students in Science and Engineering(Network Pages for Professional Development of Physicists)

Asahi Toru;Nakazato Hiromichi;Suzuki Kiyotaka

68(9) p.623 - 6272013/09-2013/09

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ISSN:00290181

Metastable Liquid Crystal as Time-Responsive Reaction Medium: Aging-Induced Dual Enantioselective Control

Y.Ishida, Y.Matsunaka, Y.Kai, K.Yamada. K.Nakagawa, T.Asahi, K.Saigo

Journal of the American Chemical Society 135(17) p.6407 - 64102013/05-

Construction of photoenergetic mitochondria in cultured mammalian cells

Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura

Scientific Reports 3(1635) 2013/04-

Selective Molecular Permeability Induced by Glass Transition Dynamics of Semicrystalline Polymer Ultrathin Films

Toshinori Fujie, Yuko Kawamoto, Hiroki Haniuda, Akihiro Saito, Koki Kabata, Yukio Honda, Eriko Ohmori, Toru Asahi, Shinji Takeoka

Macromolecules 46(2) p.395 - 4022013/01-

Selective Molecular Permeability Induced by Glass Transition Dynamics of Semicrystalline Polymer Ultrathin Films

Fujie, Toshinori;Kawamoto, Yuko;Haniuda, Hiroki;Saito, Akihiro;Kabata, Koki;Honda, Yukio;Ohmori, Eriko;Asahi, Toru;Takeoka, Shinji

MACROMOLECULES 46(2) p.395 - 4022013-2013

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ISSN:0024-9297

Construction of photoenergetic mitochondria in cultured mammalian cells

Hara, Kiyotaka Y.;Wada, Takeyoshi;Kino, Kuniki;Asahi, Toru;Sawamura, Naoya

SCIENTIFIC REPORTS 32013-2013

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ISSN:2045-2322

Metastable Liquid Crystal as Time-Responsive Reaction Medium: Aging-Induced Dual Enantioselective Control

Ishida, Yasuhiro;Matsuoka, Yuki;Kai, Yukiko;Yamada, Kuniyo;Nakagawa, Kenta;Asahi, Toru;Saigo, Kazuhiko

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135(17) p.6407 - 64102013-2013

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ISSN:0002-7863

Generalized High-Accuracy Universal Polarimeter (G-HAUP) and Its Application

T. Asahi, M. Tanaka

Polymers (The Society of Polymer Science, Japan) Vol.6p.777 - 7782012/10-

Generalized High-Accuracy Universal Polarimeter (G-HAUP) and Its Application

ASAHI Toru;TANAKA Masahito

61(10) p.777 - 7782012/10-2012/10

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Detail

ISSN:04541138

Absolute chirality of the γ-polymorph of glycine: correlation of the absolute structure with the optical rotation

Kazuhiko Ishikawa, Masahito Tanaka, Toshiya Suzuki, Akiko Sekine, Tsuneomi Kawasaki, Kenso Soai, Motoo Shiro, Meir Lehav and Toru Asahi

Chemical Communications, Communication 2012/04-

An application of the advanced high-accuracy universal polarimeter to the chiroptical measurement of an intercalated compound K4Nb6O17 with high anisotrophy

Masahito Tanaka, Naomichi Nakamura, Hideo Koshima, Toru Asahi

Journal of physics D: Applied physics 45p.1 - 72012/03-

An application of the advanced high-accuracy universal polarimeter to the chiroptical measurement of an intercalated compound K4Nb6O17 with high anisotropy

Tanaka, Masahito;Nakamura, Naomichi;Koshima, Hideko;Asahi, Toru

JOURNAL OF PHYSICS D-APPLIED PHYSICS 45(17) 2012-2012

DOIWoS

Detail

ISSN:0022-3727

Absolute chirality of the gamma-polymorph of glycine: correlation of the absolute structure with the optical rotation

Ishikawa, Kazuhiko;Tanaka, Masahito;Suzuki, Toshiya;Sekine, Akiko;Kawasaki, Tsuneomi;Soai, Kenso;Shiro, Motoo;Lahav, Meir;Asahi, Toru

CHEMICAL COMMUNICATIONS 48(48) p.6031 - 60332012-2012

DOIWoS

Detail

ISSN:1359-7345

Absolute chirality of the γ-polymorph of glycine: correlation of the absolute structure with the optical rotation.

Ishikawa Kazuhiko;Tanaka Masahito;Suzuki Toshiya;Sekine Akiko;Kawasaki Tsuneomi;Soai Kenso;Shiro Motoo;Lahav Meir;Asahi Toru

Absolute chirality of the γ-polymorph of glycine: correlation of the absolute structure with the optical rotation. 2012-2012

Detail

ISSN:1364-548X

Outline::Crystal specimens of the γ-polymorph of achiral glycine which crystallize in space groups P3(1) and P3(2) as determined by the anomalous X-ray scattering method are shown to be laevorotatory and dextrorotatory, respectively, as determined by optical rotation of the crystals.

Transparent, Tough Collagen Laminates Prepared by Oriented Flow Casting, Multi-Cyclic Vitrification and Chemical Cross-Linking

Y. Tanaka, K. Baba, T. J. Duncan, A. Kubota, T. Asahi, A. J. Quantock, M. Yamato, T. Okano, K. Nishida

Biomaterials 32p.3358 - 33662011/05-

Buffers to Suppress SDS Adsorption to PEO for Protein Separation on Capillary Polymer Electrophoresis

K. Sumitomo, K. Mayumi, H. Minamikawa, M. Masuda, T. Asahi, T. Shimizu, K. Ito, Y. Yamaguchi

Electrophoresis 32p.448 - 4542011/02-

Buffers to suppress sodium dodecyl sulfate adsorption to polyethylene oxide for protein separation on capillary polymer electrophoresis

Sumitomo, Keiko;Mayumi, Koichi;Minamikawa, Hiroyuki;Masuda, Mitsutoshi;Asahi, Toru;Shimizu, Toshimi;Ito, Kohzo;Yamaguchi, Yoshinori

ELECTROPHORESIS 32(3-4) p.448 - 4542011-2011

DOIWoS

Detail

ISSN:0173-0835

Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking

Tanaka, Yuji;Baba, Koichi;Duncan, Thomas J.;Kubota, Akira;Asahi, Toru;Quantock, Andrew J.;Yamato, Masayuki;Okano, Teruo;Nishida, Kohji

BIOMATERIALS 32(13) p.3358 - 33662011-2011

DOIWoS

Detail

ISSN:0142-9612

Improvement of Magnetic Properties and Evaluation of Read/Write Characteristics on Granular SmCo5 Perpendicular Magnetic Thin Films

T. Asahi, I. Koizumi, Y. Egawa, M. Yoshino, A. Sugiyama, J. Hokkyo, T. Kiya, J. Ariake, K. Ouchi, T. Osaka

Journal of Magnetism and Magnetic Materials 322p.3784 - 37882010/12-

Fabrication of SmCo5-CrTa Granular Films

A. Sugiyama, I. Koizumi, Y. Egawa, M. Yoshino, J. Hokkyo, T. Asahi, T. Osaka

IEEE Transaction on Magnetics 46p.1699 - 17012010/06-

Evaluation of Stability Difference between Asymmetric Homochiral Dimer in (S)-Thalidomide Crystal and Symmetric Heterochiral Dimer in (RS)-Thalidomide Crystal

T. Suzuki, M. Tanaka, M. Shiro, N. Shibata, T. Osaka, T. Asahi

Phase Transitions 83(3) p.223 - 2342010/03-

Effects of CdSe/ZnS Quantum Dots Covered Multi-Walled Carbon Nanotubes on Murine Embryonicstem Cells

D. Cui, H. Zhang, J. Sheng, Z. Wang, T. Asahi, R. He, T. Osaka, F. Gao, Hoon Sung Cho, C. Huth, H. Hu, G. M. Pauletti, D. Shi

Nano Biomedicine and Engineering 2p.236 - 2442010-

Evaluation of stability difference between asymmetric homochiral dimer in (S)-thalidomide crystal and symmetric heterochiral dimer in (RS)-thalidomide crystal

Suzuki, Toshiya;Tanaka, Masahito;Shiro, Motoo;Shibata, Norio;Osaka, Tetsuya;Asahi, Toru

PHASE TRANSITIONS 83(3) p.223 - 2342010-2010

DOIWoS

Detail

ISSN:0141-1594

Improvement of magnetic intergranular isolation and evaluation of read/write characteristics on SmCo5 perpendicular magnetic thin films

Asahi, Toru;Koizumi, Isao;Egawa, Yuko;Yoshino, Masahiro;Sugiyama, Atsushi;Hokkyo, Jiro;Kiya, Takanori;Ariake, Jun;Ouchi, Kazuhiro;Osaka, Tetsuya

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS 322(23) p.3784 - 37882010-2010

DOIWoS

Detail

ISSN:0304-8853

Single Walled Carbon Nanotubes Based Regulation of Proliferation and Differentiation of Mouse Embryonic Stem Cells

D. Cui, Z. Wang, H. Zhang, J. Ruan, C. Bao, H. Yang, T. Asahi, H. Song, K. Wang

ECS Transactions 19p.63 - 672009-

Magnetic Circular Dichroism of [Co/Pd] and [CoB/Pd] Multilayered Films

A. Agui. T. Asahi, J. Sayama, M. Mizumaki, M. Tanaka, T. Osaka

Journal of Magnetism and Magnetic Materials 320p.3015 - 30182008/11-

Improvement of Magnetic Properties and Read/Write Characteristics in SmCo5 Perpendicular Thin Films

T. Asahi, I. Koizumi, Y. Kikuchi, M. Yoshino, A. Sugiyama, J. Hokkyo, T. Osaka

Journal of Magnetisc and Magnetic Materials 320p.3075 - 30782008/11-

Effect of Growth Temperature on the Shape and Crystallinity of Chemically Produced FePt Nanoparticles

T. Hachisu, T. Yotsumoto, A. Sugiyama, H. Iida, T. Nakanishi, T. Asahi, T. Osaka

Chemistry Letters 37p.840 - 8412008/08-

Existence and Origin of Compensation Layer Thickness in Tb20Co80/Pd Multilayered Films

M. Tanaka, T. Asahi, A. Agui, M. Mizumaki, J. Sayama, T. Osaka

Journal of Physics D:Applied Physics 41p.55003 - 550062008-

Self-Assembly of Quantum Dots and Carbon Nanotubes for Ultrasensitive DNA and Antigen Detection

D. Cui, B. Pan, H. Zhang, F. Gao, R. Wu, J. Wang, R. He, T. Asahi

Analytical Chemistry 80p.7996 - 80012008-

Effect of Surface Coverage of Gold(111) Electrode with Cysteine on the Chiral Discrimination of DOPA

M. Matsunaga, T. Nakanishi, T. Asahi, T. Osaka

Chirality 19p.S36 - S392007/05-

Highly Enantioselective Discrimination of Amino Acids using Copper Deposition on a Gold Electrode Modified with Homocysteine Monolayer

M. Matsunaga, T. Nakanishi, T. Asahi, T. Osaka

Electrochmistry Communications 9p.725 - 7282007/04-

Sputter-Deposited SmCo5 Thin Films with Perpendicular Magnetic Anisotropy for High Density Magnetic Recording Media

J. Sayama, Y. Yamashita, T. Asahi, T. Osaka

Magnetic Materials, Processes, and Devices9 ECS Transaction 3p.307 - 3132007-

Enantioselectivity of Redox Reaction of DOPA at the Gold Electrode Modified with a Self-assembly Monolayer of Homocycteine

T. Nakanishi, M. Matsunaga, M. Nagasaka, T. Asahi, T. Osaka

Journal of American Chemical Society 128p.13322 - 133232006/10-

Soft X-ray Absorption Spectroscopy and Magnetic Circular Dichroism Study of Electroless-deposited CoNiFe Ternary Alloy Soft Magnetic Film

M. Tanaka, N. Nakamura, T. Asahi, T. Tsumori, A. Agui, M. Mizumaki, T. Osaka

Physica Status Solidi 3p.2783 - 27862006/09-

Crystalline of Leucine on a self-assembled Monolayer with Covalently Attached Enantiomeric Leucine Molecules

T. Nakanishi, N. Banno, M. Matsunaga, T. Asahi, T. Osaka

Colloids and Surfaces A: Physicochemical and Engineering Aspects 284p.270 - 2752006/08-

Electrochemical Formation of Intermediate Layer for Co/Pd Multilayered Media

J. Kawaji, K. Kimura, T. Asahi, T. Homma, T. Osaka

Journal of Magnetism and Magnetic Materials 303p.128 - 1322006/08-

Study on N2-added and B-doped effect in Co/Pd multilayered films using soft x-ray absorption and magnetic circular dichroism

A. Agui, T. Asahi, J. Sayama, J. Kawaji, M. Mizumaki, M. Tanaka, T. Osaka

Jornal of Physics D: Applied Physics 39p.2881 - 28852006/07-

Origin of Perpendicular Magnetic Anisotropy of SmCo5 Thin Films with Cu Underlayer

J. Sayama, K. Mizutani, T. Asahi, J. Ariake, K. Ouchi, T. Osaka

Journal of Magnetisc and Magnetic Materials 301p.271 - 2782006/06-

Effect of Substrate Morphology on the Crystallization of Leucine on Gold Surface Modified with a Self-assembled Monolayer

T. Osaka, M. Matsunaga, T. Nakanishi, T. Asahi, N. Banno

Chemistry Letters 35p.438 - 4392006/04-

Incident Angle Dependence of MCD at the Dy M5-Edge of Perpendicular Magnetic DyxCo100?x Films

A. Agui, M. Mizumaki, T. Asahi, J. Sayama, K. Matsumoto, T. Morikawa, T. Matsushita, T. Osaka, Y. Miura

Journal of Alloys and Compounds 408p.741 - 7452006/02-

Magnetic Properties and Domain Structure of SmCo5 Perpendicular Magnetization Films Prepared by Using a UHV Sputtering System

J. Sayama, T. Asahi, Y. Yamashita, J. Ariake, K. Ouchi, T. Osaka

Journal of the Magnetics Society of Japan 30p.423 - 4282006-

SmCo5 Thin Films with High Magnetic Anisotropy for Perpendicular Magnetic Recording

J. Sayama, K. Mizutani, Y. Yamashita, T. Asahi, T. Osaka

IEEE Transactions on Magnetics 41p.3133 - 31352005/10-

Optical Activity Induced by Helical Arrangements of Tryptamine and 4-Chlorobenzoic Acid in Their Cocrystal

H. Koshima, M. Nagano, T. Asahi

Journal of American Chemical Society 127p.2455 - 24632005/04-

Effect of N2 Additive Gas during Sputtering on Magnetic Properties and Microstructure of CoB/Pd Multilayered Media

T. Asahi, M. Tanaka, J. Sayama, J. Kawaji, S. Matsunuma, T. Osaka

Journal of Magnetisc and Magnetic Materials 287p.199 - 2032005/02-

Electroless-Deposited Soft Magnetic Underlayer on Silicon Disk Substrate for Double-Layered Perpendicular Magnetic Recording Media

T. Osaka, T. Asahi, T. Yokoshima, J. Kawaji

Journal of Magnetism and Magnetic Materials 287p.292 - 2972005/02-

Magnetic Properties and Microstructure of SmCo5 Thin Film with Perpendicular Magnetic Anisotropy

J. Sayama, K. Mizutani, T. Asahi, J. Ariake, K. Ouchi, S. Matsunuma, T. Osaka

Journal of Magnetism and Magnetic Materials 287p.239 - 2442005/02-

Microstructure of a Co/Pd Multilayered Perpendicular Recording Medium with Pd Seeds Prepared by Electrochemical Process

M. Tanaka, J. Kawaji, K. Kimura, T. Asahi, T. Homma, S. Matsunuma, T. Osaka

Journal of Magnetism and Magnetic Materials 287p.188 - 1922005/02-

Electroless Deposition of Soft Magnetic Underlayer for a Double-layered Perpendicular Recording Media

A. Sugiyama, T. Asahi, K. Adachi, M. Sakagami, J. Kawaji, M. Yoshino, T. Osaka, T. Tsumori

IEICE Technical Report MR2004-48p.15 - 202005-

Application of Soft X-ray Magnetic Circular Dichroism Spectroscopy to the Investigation of [Co/Pd] Multilayered Perpendicular Magnetic Recording Media with Various Seedlayers

A. Agui, M. Mizumaki, T. Matsushita, T. Asahi, J. Kawaji, J. Sayama, T. Osaka

Journal of Applied Physics 95p.7825 - 78312004/06-

Chiral Discrimination Between Thalidomide Enantiomers Using a Solid Surface With Two-Dimensional Chirality

T. Nakanishi, N. Yamakawa, T. Asahi, N. Shibata, B. Ohtani, T. Osaka

Chirality 16p.S36 - S392004/06-

Enantioselective Crystal Growth of Leucine on a Self-Assembled Monolayer with Covalently Attached Leucine Monolayers

N. Banno, T. Nakanishi, M. Matsunaga, T. Asahi, T. Osaka

Journal of American Chemical Society 126p.428 - 4292004-

Formation of Pd Nanocluster Seed by Electrochemical Process for Control of Magnetic Properties in Co/Pd Multilayered Film

J. Kawaji, M. Tanaka, K. Kimura, T. Asahi, T. Homma, T. Osaka

IEEE Transactions on Magnetics 40p.2473 - 24752004-

Magnetic Properties of SmCo5 Perpendicular. Magnetization Film with Cu Seedlayer

J. Sayama, K. Mizutani, T. Asahi, T. Osaka

IEICE Technical Report MR2004-8p.7 - 112004-

Microstructure and Magnetic Properties of a Co/Pd Multilayer on a Controlled Pd/si Seedlayer for Double-Layered Perpendicular Magnetic Recording media

J. Kawaji, T. Asahi, H. Hashimoto, J. Hokkyo, S. Matsunuma, G. Safran, J. Ariake, K. Ouchi, T. Osaka

Journal of Applied Physics 95p.8023 - 80292004-

Newly developed SmCo5 thin film with perpendicular magnetic anisotropy

J. Sayama, T. Asahi, K. Mizutani, T. Osaka

Journal of Physics D: Applied Physics 37p.1 - 42004-

Soft x-ray Magnetic Circular Dichroism Study of [Co/Pd] Multilayered Perpendicular Magnetic Films

A. Agui, M. Mizumaki, T. Matsushita, T. Asahi, J. Kawaji, J. Sayama, T. Osaka

Journal of Applied Physics 95p.7825 - 78312004-

Thin films of SmCo5 with very high perpendicular magnetic anisotropy

J. Sayama, K. Mizutani, T. Asahi, T. Osaka

Applied Physics Letters 85p.5640 - 56422004-

MCD Measurement at the Tb M4,5-edges of Tb17FexCo(83-x) Perpendicular Magnetization Films

A. Agui, M. Mizumaki, T. Asahi, J. Sayama, K. Matsumoto, T. Morikawa, T. Nakatani, T. Matsushita, T. Osaka, Y. Miura

Transactions of the Magnetics Society of Japan 4p.326 - 3292004-

Optimization of the Thickness Ratio of Pd/Si Intermediate Layer in a Co/Pd Multilayer Perpendicular Magnetic Recording Media

J. Kawaji, H. Hashimoto, T. Asahi, J. Hokkyo, T. Osaka

Transactions of the Magnetics Society of Japan 4p.78 - 842004-

Measurement of Circular Dichroism of Ferroelectric Fresnoite Ba2Si2TiO8

T. Asahi, T. Osaka, J. Kobayashi

Proceedings of SPIE 5218p.223 - 2322003-

Enantioselective Adsorption of Phenylalanine onto Self-Assembled Monolayers of 1, 1’-Binaphthalene-2, 2’-dithiol on Gold

T. Nakanishi, N. Yamakawa, T. Asahi, T. Osaka, B. Ohtani, K. Uosaki

Journal of American Chemical Society 124p.740 - 7412002-

Enantioselective adsorption of phenylalanine onto self-assembled monolayers of 1,1 '-binaphthalene-2,2 '-dithiol on gold

T. Nakanishi, N. Yamakawa, T. Asahi, T. Osaka, B. Ohtani, K. Uosaki

Journal of American Chemical Society 124p.740 - 7412002-

Enhancement of Magnetic Properties of Co/Pd Multilayered Perpendicular Magnetic Recording Media by Using Pd/Si Dual Seedlayer

J. Kawaji, T. Asahi, T. Onoue, J. Sayama, J. Hokkyo, T. Osaka, K. Ouchi

Journal of Magnetism and Magnetic Materials 251p.220 - 2282002-

Microstructure and Magnetic Properties of Co/Pd Multilayered Thin Films with C or Si Seedlayer

T. Onoue, T. Asahi, K. Kuramochi, J. Kawaji, T. Osaka, J. Ariake, K. Ouchi, G. Safran, N. Yaguchi

Journal of Applied Physics 92p.4545 - 45522002-

Thermal Deposition High-Resolution Mass Spectroscopy of mixed Self-Assmbled Monolayers on Gold

T. Shibue, T. Nakanishi, T. Matsuda, T. Asahi, T. Osaka

Langmuir 18p.1528 - 15342002-

Absolute Configuration of L-(+)589-Tris(Ethylenediamine) Cobalt(III) Triiodide Monohydrate

R. Matsuki, T. Asahi, H. Asai, M. Shiro

Acta Crystallographica E 57p.1 - 32001-

Analysis of Microstructure for Co/Pd Multilayer Perpendicular Magnetic Recording Media with Carbon

T. Asahi, K. Kuramochi, J. Kawaji, T. Onoue, T. Osaka

Journal of Magnetism and Magnetic Materials 235p.87 - 922001-

CoCrPtTa and Co/Pd Perpendicular Magnetic Recording Media with Amorphous Underlayers

T. Onoue, T. Asahi, K. Kuramochi, J. Kawaji, T. Homma, T. Osaka

IEEE Transaction on Magnetics 37p.1592 - 15942001-

Effect of Underlayers on Magnetic Properties of a Co/Pd Multilayer Perpendicular Magnetic Recording Medium

T. Onoue, J. Kawaji, K. Kuramochi, T. Asahi, T. Osaka

Journal of Magnetism and Magnetic Materials 235p.82 - 862001-

Enantioselective Fluorination Mediated by Cinchona Alkaloid Derivatives / Selectfluor Combinations: Reaction Scope and Structural Information of N-Fluorocinchona Alkaloids

N. Shibata, E. Suzuki, T. Asahi, M. Shiro

Journal of American Chemical Society 123p.7001 - 70092001-

Improvement of Signal to Noise Ratio for Co/Pd Multilayer Perpendicular Magnetic Recording Media by the Addition of an Underlayer

T. Onoue, T. Asahi, K. Kuramochi, J. Kawaji, T. Homma, T. Osaka

Journal of Magnetism and Magnetic Materials 235p.40 - 442001-

Optical Study of a Phase Transition of Fresnoite

T. Asahi, T. Osaka, J. Kobayashi, S. C. Abrahams, S. Nanamatsu, M. Kimura

Physical Review B 63p.1 - 132001-

Polarimetry of Electromagnetic Materials

T. Asahi, T. Osaka, J. Kobayashi

Proceedings of SPIE 4467p.20 - 302001-

Relationship Between Surface Energy and Preferred Orientation of CoCr Alloy Magnetic Thin Films Deposited on C and TiCr

H. Okuo, T. Asahi, T. Onoue, T. Osaka

Journal of Magnetism and Magnetic Materials 235p.98 - 1032001-

Analysis for Magnetized State of Perpendicular Magnetic Recording Media Using MFM

M. Kawakami, T. Onoue, T. Asahi, J. Hokkyo, T. Osaka

Journal of the Magnetics Society of Japan 25p.571 - 5742001-

Changes of Microstructure Versus Film Thickness in CoCrPtTa/NiCr Media

K. Kurasawa, M. Kawakami, T. Onoue, T. Asahi, T. Osaka

Journal of the Magnetics Society of Japan 25p.623 - 6262001-

Preparation of Co/Pd Multilayer Perpendicular Magnetic Recording Media with Carbon Under Layer

T. Asahi, K. Kuramochi, J. kawaji, T. Onoue, T. Osaka

Journal of the Magnetics Society of Japan 25p.575 - 5782001-

Preparation of a Perpendicular/Longitudinal Composite Medium with a Silicon Intermediate Layer

Y. Morita, T. Onoue, T. Asahi, T. Honma, T. Osaka

Journal of the Magnetics Society of Japan 25p.619 - 6222001-

Application of Si Amorphous Underlayers to CoCrPtTa Perpendicular Magnetic Recording Media

T. Asahi, T. Onoue, M. Ikeda, Y. Morita, T. Osaka

Ferrites: Proceedings of the 8th International Conference on Ferrite p.1055 - 10572000-

CoCrPtTa Single Layer Perpendicular Magnetic Recording Media with Carbon Underlayer

T. Onoue, A. Takizawa, T. Asahi, T. Osaka

Journal of Applied Physics 88p.6645 - 66512000-

Development of HAUP and Its Applications to Various Kinds of Solids

J. Kobayashi, T. Asahi

Proceedings of SPIE 4097p.25 - 392000-

Influence of Substrates on Magnetic Property and Crystalline Orientation of CoCrTa/TiCr Perpendicular Magnetic Recording Medium

T. Asahi, M. Ikeda, A. Takizawa, T. Onoue, T. Osaka

Journal of Magnetism & Magnetic Materials 212p.293 - 2992000-

Analysis of the Remanent Magnetization State at Track Edges for a Ring-Type Head and Single-Layered Perpendicular Magnetic Recording Medium

T. Hirayama, T. Onoue, T. Asahi, T. Honma, J. Hokkyo, T. Osaka

Journal of the Magnetics Society of Japan 24p.223 - 2262000-

Fabrication of a Single-Layered Perpendicular Magnetic Recording Medium with a Ge Underlayer

Y. Morita, K. Kuramochi, T. Onoue, T. Asahi, T. Osaka

Journal of the Magnetics Society of Japan 24p.271 - 2742000-

Preparation of a Perpendicular/Longitudinal Composite Medium with a Ni-Alloy Longitudinal Magnetic Underlayer

M. Kawakami, K. Kurasawa, M. Ikeda, T. Onoue, T. Asahi, T. Osaka

Journal of the Magnetics Society of Japan 24p.263 - 2662000-

Effect of a Paramagnetic CoCr40 Intermediate Layer on Magnetic Properties and Crystalline Structure of the CoCr18Pt6Ta3 Perpendicular Magnetic Recording Medium with an Amorphous C Underlayer

T. Asahi, T. Onoue, A. Takizawa, T. Osaka

Electrochemical Society Proceedings 99-34p.304 - 3141999-

Fabrication of a Perpendicular/Longitudinal Composite Medium by Sputtering

A. Takizawa, T. Onoue, M. Ikeda, T. Hirayama, T. Asahi, T. Osaka, H. Sakai

Journal of the Magnetics Society of Japan 23p.973 - 9761999-

Study on Medium Noise and Remanent Magnetization State for Single-Layer Perpendicular Magnetic Recording Media

T. Onoue, T. Asahi, T. Hirayama, J. Hokkyo, T. Osaka

Journal of the Magnetics Society of Japan 23p.45 - 481999-

Optical Study on Phase Transition of Pb0.92La0.08(Zr0.65Ti0.35) 0.89O3

T. Asahi, Y. Itagaki, H. Utsumi, H. Yoshii, I. Kamiya, M. Ichiki, M. Takahashi, J. Kobayashi

Ferroelectrics 208p.459 - 4691998-

The Optical Activity of Lysozyme Crystal

J. Kobayashi, T. Asahi, M. Sakurai, I. Kagomiya, H. Asai, H. Asami

Acta Crystallographica A 54p.581 - 5901998-

Optical Activity of Oxo Amide Crystal

T. Asahi, M. Nakamura, J. Kobayashi, F. Toda, H. Miyamoto

Journal of American Chemical Society 119p.3665 - 36691997-

The Optical Activity of Crystalline L-Aspartic Acids

T. Asahi, M. Takahashi, J. Kobayashi

Acta Crystallographica A 53p.763 - 7711997-

Thermal Expansion of Superconducting Bi2Sr2CaCu2O8

T. Asahi, H. Suzuki, M. Nakamura, H. Takano, J. Kobayashi

Physical Review B 55p.9125 - 91291997-

Optical Activity of Crystalline Glutamic Acids

T. Asahi, H. Utsumi, Y. Itagaki, I. Kagomiya, J. Kobayashi

Acta Crystallographica A 52p.766 - 7691996-

Optical Activity of Poly-L-Lactic Acids in Crystalline and Dissolved States

M. Ichiki, T. Asahi, J. Kobayashi

Phase Transitions 56p.67 - 781996-

Optical Properties of Superconducting Bi2Sr2CaCu2O8

J. Kobayashi, T. Asahi, M. Sakurai, M. Takahashi, K. Okubo, Y. Enomoto

Physical Review B 53p.11784 - 117951996-

Optical and X-ray Study on Phase Transition of (NH4)2SO4

T. Asahi, R. Ikeda, M. Higano, H. Suzuki, M. Nakamura, T. Morikawa, J. Kobayashi

Ferroelectrics Letters 21p.47 - 531996-

Optical Activity of Solid Polymer

J. Kobayashi, T. Asahi, M. Ichiki, A. Oikawa

Ferroelectrics 171p.69 - 941995-

Optical Study on Phase Transition of Lead Lanthanum Zirconate Titanate Pb0.92La0.08(Zr0.70Ti0.30)0.98O3 ceramics

J. Kobayashi, T. Asahi, M. Ichiki, K. Saito, T. Shimazaki, H. Yoshii, Y. Itagaki, H. Ikawa

Physical Review B 51p.763 - 7781995-

Structural and Optical Properties of Poly Lactic Acids

J. Kobayashi, T. Asahi, M. Ichiki, A. Oikawa, H. Suzuki, T. Watamabe, E. Fukada, Y. Shikinami

Journal of Applied Physics 77p.2957 - 29731995-

Optical Activity in Poly-L-Lactic Acid

J. Kobayashi, M. Ichiki, T. Watamabe, T. Asahi, A. Oikawa, H. Suzuki

Ferroelectrics 151p.75 - 841994-

Optical Activity of (NH4) 2SO4

M. Higano, T. Asahi, Y. Sato, K. Okubo, J. Kobayashi

Ferroelectrics 152p.285 - 2871994-

Optical Activity of Gd2 (MoO4) 3

K. Saito, T. Asahi, N. Takahashi, M. Higano, I. Kamiya, Y. Sato, K. Okubo, J. Kobayashi

Ferroelectrics 152p.231 - 2361994-

Optical Propreties of PLZT

M. Ichiki, T. Shimasaki, H. Yoshii, T. Asahi, J. Kobayashi, H. Ikawa

Ferroelectrics 152p.289 - 2931994-

Optical Activity and Phase Transitions of Pb1-xLax(ZryTiz) 1-x/4O3

H. Yoshii, T. Shimazaki, M. Ichiki, Y. Itagaki, T. Asahi, K. Saito, J. Kobayashi, H. Ikawa

Japanese Journal of Applied Physics 33p.5435 - 54391994-

Optical Properties of BaMnF4

T. Asahi, M. Tomizawa, J. Kobayashi, W. Kleemann

Physical Review B 45p.1971 - 19871992-

Optical Properties of Gd2 (MoO4) 3 Studied by High Accuracy Universal

T. Asahi, M. Higano, Cao Fu-Tian, J. Kobayashi

Japanese Journal of Applied Physics 31p.3214 - 32171992-

Optical Study of Pb1-xLax(ZryTiz) 1-x/4O3

M. Ichiki, T. Asahi, T. Shimasaki, J. Kobayashi, H. Ikawa

Japanese Journal of Applied Physics 31p.3212 - 32131992-

Optical and Structural Studies of Poly-Lactic Acid (I)

M. Ichiki, T. Asahi, T. Watanabe, J. Kobayashi, E. Fukada

Reports on Progress in Polymer Physics in Japan 35p.441 - 4441992-

Optical and Structural Studies of Poly-Lactic Acid (II)

M. Ichiki, T. Asahi, T. Watanabe, J. Kobayashi, E. Fukada

Reports on Progress in Polymer Physics in Japan 35p.445 - 4481992-

Optical Properties of Rochelle Salt

J. Kobayashi, K. Uchino, T. Asahi

Physical Review B 43p.5706 - 57121991-

Optical Activity of BaMnF4

T. Asahi, K. Uchino, M. Tomizawa, J. Kobayashi, W. Kleemann

Ferroelectrics 105p.213 - 2171990-

Optical Activity of Poly-L-Lactic Acid

T. Asahi, H. Fukase, M. Ichiki, J. Kobayashi, E. Fukada

Ferroelectrics 109p.339 - 3421990-

The Optical Study of BaMnF4 in Incommensurate Phase

T. Asahi, K. Uchino, J. Kobayashi, W. Kleemann

Ferroelectrics 94p.329 - 3351989-

Evaluation of the Systematic Errors of Polarimetric Measurements: Application to Measurement of the Gyration Tensor of α-Quartz

J. Kobayashi, T. Asahi, S. Takahashi, A. M. Glazer

Journal of Applied Crystallography 21p.479 - 4841988-

Simultaneous Measurement of Electrogyration and Electrooptic Effects ofα-Quartz

J. Kobayashi, T. Asahi, S. Takahashi

Ferroelectrics 75p.139 - 1521987-

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes

Kenta Nakagawa, Heather Harper-Lovelady, Yuji Tanaka, Masahito Tanaka, Masayuki Yamato, Toru Asahi

Chemical Communications 50(95) p.15086 - 15089

Books And Publication

「異方性媒質のキラル光学/磁気光学の精密測定 」

朝日透

磁気学会2013-

CSJカレントレビュー13「キラル化学-その起源から最新のキラル材料研究まで」

朝日透,田中真人

化学同人2013/10-

生命科学概論

朝日透、池田康夫、石井義孝、井上貴文、大坂利文、大島登志男、岡村好子、合田亘人、澤村直哉、重谷安代、仙波憲太郎、武岡真司、武田直也、竹山春子、常田聡、増田優、南沢享

朝倉出版2012-

Magnetic Materials, Processes, and Devices 9 : ECS Transaction Vol. 3 No.25

J. Sayama, Y. Yamashita, T. Asahi, T. Osaka

2007-

Introduction to Complex Mediums for Optics and Electromagnetics SPIE PRESS

T. Asahi and J. Kobayashi

2003-

SPIE Press

T. Asahi and J. Kobayashi

2003-

今日からモノ知りシリーズ トコトンやさしいナノテクノロジーの本

大泊 巌(編著)

2002/03-

Lecture And Oral

Microstructure and Magnetic Properties of Co/Pd Multilayered thin films with C or Si seedlayer

2002/01

Patent

Reference Number:160

磁気記録媒体の製造方法(日本)

朝日 透, 川治 純, 逢坂 哲彌

2002- 82981、2003-281711、4138348

Reference Number:164

磁気記録媒体、その製造方法及び磁気記憶装置(日本)

逢坂 哲彌, 朝日 透, 横島 時彦

2002-314949、2004-152367

Reference Number:323

バイオセンシング方法及び固定化方法(日本)

逢坂 哲彌, 横島 時彦, 朝日 透, 松永 是, 新垣 篤史, 丹羽 大輔

2003-321535、2005- 91014、4257513

Reference Number:344

垂直磁気記録媒体の製造方法(日本)

逢坂 哲彌, 朝日 透, 横島 時彦

2003-373140、2004-335068、4408210

Reference Number:392

量子化コンダクタンス素子、これを用いた磁場変化検出方法及び磁気検出方法、並びに量子化コンダクタンス素子の製造方法(日本)

逢坂 哲彌, 大島 忠平, 朝日 透, 六田 英治

2004-097445、2005-286084

Reference Number:420

Sm-Co合金系垂直磁気異方性薄膜およびその形成方法(日本)

佐山 淳一, 逢坂 哲彌, 朝日 透

2004- 85370、2005-109431、4614046

Reference Number:448

電子光学装置用電子ビーム源及びその製造方法(日本)

逢坂 哲彌, 大島 忠平, 朝日 透, 六田 英治, 板垣 孝洋

2004-320442、2006-134638、4543129

Reference Number:467

磁気記録媒体及び磁気記録媒体基板(日本)

逢坂 哲彌, 朝日 透, 横島 時彦

2004-265690、2005-108407

Reference Number:504

金属針構造体及びその製造方法(日本)

逢坂 哲彌, 大島 忠平, 朝日 透, 六田 英治, 郭 鴻曦, 鄭 天佐

2005-109563、2006-294283、4742187

Reference Number:796

磁気記録媒体(日本)

逢坂 哲彌, 朝日 透, 川治 純

2007-334367、2008-091026

Reference Number:809

磁気記録媒体用基板及び磁気記録媒体(日本)

逢坂 哲彌, 朝日 透, 横島 時彦

2007-339512、2008- 97822

Reference Number:867

磁気異方性垂直磁化膜及びその形成方法並びに磁気記録媒体及びその製造方法(日本)

逢坂 哲彌, 朝日 透, 杉山 敦史, 吉野 正洋, 小泉 公

2008-184416、2010- 27108、5177407

Reference Number:935

バイオセンシング方法及び固定化方法(日本)

逢坂 哲彌, 松永 是, 新垣 篤史, 朝日 透, 横島 時彦, 丹波 大介

2008-307030、2009-103703、4911639

Research Grants & Projects

Grant-in-aids for Scientific Research Adoption Situation

Research Classification:

Reverse reaction of thalidomide hydrolytic product to thalidomide for reconsidering metabolic pathway of thalidomide

2014/-0-2016/-0

Allocation Class:¥3900000

Research Classification:

Functional modification of live cells by focused ion beam doping

2012/-0-2014/-0

Allocation Class:¥4030000

Research Classification:

Constructing cDNA for hypothesis verification of crystal melting of Ca2+driven contractile protein

2011/-0-2014/-0

Allocation Class:¥3640000

Research Classification:

STUDY ON RECORDING CHARACTERISTICS OF MULTILAYERED THIN FILM MAGNETIC RECORDING MEDIA

2001-2003

Allocation Class:¥3200000

Research Classification:

Study of recording and reproducing process in high density magnetic recording

1998-2000

Allocation Class:¥3100000

Research Classification:

New Type Catheter Using Photomechanical Crystal Actuator

2016/-0-2018/-0

Allocation Class:¥3510000

Research Classification:

Toward the implementation of evidence-based health policies to the real world-a trial of using big data analyses by the integration of arts and sciences

2019/-0-2023/-0

Allocation Class:¥25480000

On-campus Research System

Special Research Project

精神神経疾患の診断と病態解明を目指したバイオセンサの開発

2013

Research Results Outline:特定課題B「精神神経疾患の診断と病態解明を目指したバイオセンサの開発」では、FETバイオセンサを用いて、センサ検出部に修飾されている有機薄膜とAmyl特定課題B「精神神経疾患の診断と病態解明を目指したバイオセンサの開発」では、FETバイオセンサを用いて、センサ検出部に修飾されている有機薄膜とAmyloid ßタンパク質(Amyloid ß protein; Aβ)との相互...特定課題B「精神神経疾患の診断と病態解明を目指したバイオセンサの開発」では、FETバイオセンサを用いて、センサ検出部に修飾されている有機薄膜とAmyloid ßタンパク質(Amyloid ß protein; Aβ)との相互作用に基づいた検出機構について、先端電位変化検出手法を用いて解明する事を目的として研究を行った。さらに、精神神経疾患の診断へ応用できるバイオセンサの実用化目指すとともに、確立したバイオセンサを用いて、精神神経疾患モデル神経細胞や患者サンプルでのアミロイドß蛋白の分泌量を測定し、病態を解明することを目指して研究を行った。本研究では、クロスβ構造を有するアミロイド凝集体と特異的な相互作用を示すコンゴーレッドでゲート表面を修飾したFETによるAβ(1-42)凝集体の検出を試みた。Aβ(1-42)の検出はFET特性(ゲート電圧-ドレイン電流特性)の閾値電圧シフトとして評価した。Aβ(1-42)のモノマーを数日インキュベートして得た試料では、凝集体を形成した場合にのみFET応答が観測された。また、Aβ(1-42)とその亜種であるAβ(1-40)に対する応答を比較したところ、Aβ(1-42)検出が可能なインキュベート日数であっても、凝集能の低いAβ(1-40)にはFET応答は観測されず、コンゴーレッド修飾FETのAβ凝集体への特異的な応答が示唆された。本研究成果は今年2月にChemical Communications に掲載され、日本経済新聞にも取り上げられた。参考文献Hideshima, S., Kobayashi, M., Wada, T., Kuroiwa, S., Nakanishi, T., Sawamura, N., Asahi T., Osaka, T.A label-free electrical assay of fibrous amyloid β based on semiconductor biosensing. Chemical Communications 50, 3476-3479 (2014)2014.3.25日本経済新聞夕刊 014ページ 「アルツハイマー病の原因物質,短時間で検出 早大が開発」

異方性物質の磁気キラル光学的研究

2014Collaborator:Garcia Villora, 島村清史

Research Results Outline: Faradayrotator single-crystals have attracted much attention in the f Faradayrotator single-crystals have attracted much attention in the field ofmagneto-optics due to their broad applicat... Faradayrotator single-crystals have attracted much attention in the field ofmagneto-optics due to their broad applications such as optical isolators,optical modulators, and beam splitters. Rare-earth (RE) fluorides (CeF3, PrF3 and LiREF4)single-crystals have been investigated due to their unique transmittance in theUV wavelength region.  Themagneto-optical effects of the RE ions are known to be caused by the intraionicparity allowed electric dipole transition between the 4fN and 4fN-15d1configurations. These transitions are close to the absorption cut-off in thewidely transparent fluorides, so that these achieve outstanding Verdetconstants in the UV wavelength region.   Accurate Faraday rotationmeasurements have been carried out along the optical c-axis in order to avoid the linear birefringence.  However, to investigate the optical and magnetic properties of the REfluorides in more detail, measurements along the perpendicular direction arerequired.  In this study, the wavelength dependencesof the linear and circular birefringences of CeF3 single-crystalalong the a-axis were measured withthe Generalized-High Accuracy Universal Polarimeter (G-HAUP).

銅酸化物高温超伝導体Bi2Sr2CaCu2O8+xの光学的性質と対称性の破れ

2016Collaborator:松田梓, 中川鉄馬, 篠元輝

Research Results Outline:最適ドープ・アンダードープのBi系銅酸化物高温超伝導体Bi2Sr2CaCu2O8+δ (Bi2212) 単結晶をフローティング法を用いて育成し,その極最適ドープ・アンダードープのBi系銅酸化物高温超伝導体Bi2Sr2CaCu2O8+δ (Bi2212) 単結晶をフローティング法を用いて育成し,その極薄試料をそのc軸方向の劈開性を利用して作成した。また,常温における光学活性,自然円二色性,Fara...最適ドープ・アンダードープのBi系銅酸化物高温超伝導体Bi2Sr2CaCu2O8+δ (Bi2212) 単結晶をフローティング法を用いて育成し,その極薄試料をそのc軸方向の劈開性を利用して作成した。また,常温における光学活性,自然円二色性,Faraday回転,磁気円二色性,直線複屈折,直線二色性の波長依存性を高精度万能旋光計 (G-HAUP) により測定した。それら符号と大きさを比較することにより,銅酸化物高温超伝導体における超伝導発現機構の解明につながると考えられる擬ギャップ相における空間・時間反転対称性の破れの存否を直接的に検証することが可能となった。

異方性二次元キラルナノ構造体の可逆・非可逆光学現象の検証

2016Collaborator:成島哲也, 中川鉄馬

Research Results Outline:二次元で鏡面対称 (線対称) を有さないナノメートルサイズの形状を持つ「S」などの構造を異方的に整列させた集合体「異方性二次元キラルナノ構造体 二次元で鏡面対称 (線対称) を有さないナノメートルサイズの形状を持つ「S」などの構造を異方的に整列させた集合体「異方性二次元キラルナノ構造体 (2D-CNS)」が、直線偏光の偏光面を回転させる性質「旋光性」や楕円偏光化させる性質「円二色性...二次元で鏡面対称 (線対称) を有さないナノメートルサイズの形状を持つ「S」などの構造を異方的に整列させた集合体「異方性二次元キラルナノ構造体 (2D-CNS)」が、直線偏光の偏光面を回転させる性質「旋光性」や楕円偏光化させる性質「円二色性」を示すか否かを我々が独自に開発してきた「高精度万能旋光計 (G-HAUP)」を用いて可逆あるいは非可逆現象の検証を含め調べ、その発現起因 (空間反転対称性の破れ、時間反転対称性の破れ) にまで踏み込んで考察した。本研究成果は、光と物質の相互作用過程に、試料の構造や形状などの二次元的な非対称性がどのように関与しているのかを理解することにつながる。

強誘電性結晶硫酸トリグリシンのキラリティ制御機構の解明

2018

Research Results Outline:キラル分子であるL体およびD体のアラニン、乳酸、スレオニン、セリンを硫酸トリグリシン(Triglycine sulfate ; TGS)にドープした結キラル分子であるL体およびD体のアラニン、乳酸、スレオニン、セリンを硫酸トリグリシン(Triglycine sulfate ; TGS)にドープした結晶において、アラニンドープTGS(alanine-doped TGS ; ATGS)のみ、アラニン...キラル分子であるL体およびD体のアラニン、乳酸、スレオニン、セリンを硫酸トリグリシン(Triglycine sulfate ; TGS)にドープした結晶において、アラニンドープTGS(alanine-doped TGS ; ATGS)のみ、アラニンのキラリティによってTGS結晶のキラリティが偏ることを見出した。また、ATGSの結晶構造を決定し、アラニンがTGS結晶のキラリティを決定する分子と置換する可能性があることが分かり、アラニンのキラリティによってTGS結晶のキラリティが制御される機構の解明の手掛かりを得た。

キラル・磁気光学ハイブリッド顕微鏡の開発

2007Collaborator:田中真人

Research Results Outline:物質のキラリティの研究は、アミノ酸、タンパク質、DNAなどの生体物質の構造と機能性の関係の解明、薬剤の効用と副作用の研究などに有用となる物質科学におけ物質のキラリティの研究は、アミノ酸、タンパク質、DNAなどの生体物質の構造と機能性の関係の解明、薬剤の効用と副作用の研究などに有用となる物質科学における緊要な学際的研究である。われわれは、直線複屈折、直線2色性、円複屈折、円2色性を同時に測定できる...物質のキラリティの研究は、アミノ酸、タンパク質、DNAなどの生体物質の構造と機能性の関係の解明、薬剤の効用と副作用の研究などに有用となる物質科学における緊要な学際的研究である。われわれは、直線複屈折、直線2色性、円複屈折、円2色性を同時に測定できる光学測定法(拡張HAUP法)を開発し、2それら異方的光学物理量の分光学的測定及び温度依存性の測定を可能とした「一般型高精度万能旋光計」(Generalized-HAUP、略称、G-HAUP)の開発に成功した。本研究では、その測定原理をイメージング技術に応用して、新しい顕微鏡「キラル・磁気光学ハイブリッド顕微鏡」を創設ために不可欠な光学系の改良ならびに測定プログラムの開発やその実証に必要な実験を遂行することとした。さらに、シンクロトロン放射光を用いた「キラル・磁気光学ハイブリッド顕微鏡」の開発への試験的な研究に着手することとした。まずは、キラル光学効果の高分解能分光測定及び2次元イメージングのできる「透過型高分解能キラリティ顕微鏡」を試作するため、G-HAUPの光学系及びその駆動系、試料台、検出器、ノイズ低減機構、測定及びデータ解析プログラムを改良した。次いで、その新しい光学系と測定プログラムを用いて、サリドマイドのキラル反転機構の解明に資するキラル光学的研究を行った。サリドマイドはキラルな分子構造を有するきわめて有用な薬剤であるが,溶液状態ではR体からS体,S体からR体へキラル反転することが指摘され、その機構は未だ十分理解されていない。一方、サリドマイドのキラル光学性質を測定し、その結果の正当性を確保するためには、サリドマイドのキラル結晶の構造を決定することが重要である。そこで、まず、我々はこれまで明らかとなっていないサリドマイドのキラル結晶につき、X線回折法を用いて構造解析した。サリドマイドの結晶はキラルな点群C2となり、2つのサリドマイド分子が環状水素結合して安定な構造を有していることが明らかとなった。さらに,G-HAUPを用いてサリドマイドのキラル結晶の光学的性質の波長依存性を調べたところ、再現性を確認する必要はあるものの、直線複屈折、直線2色性、円複屈折、円2色性の異常を見い出すことができた。

Lecture Course

Course TitleSchoolYearTerm
Molecular Biology 51School of International Liberal Studies2019fall semester
Basic Experiments in Science and Engineering 2B KagakuSchool of Advanced Science and Engineering2019fall semester
Basic Experiments in Science and Engineering 2B SeiiSchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bio-science Seminar ISchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Seminar I [S Grade]School of Advanced Science and Engineering2019spring semester
Basic StatisticsSchool of Advanced Science and Engineering2019spring semester
Basic StatisticsSchool of Advanced Science and Engineering2019spring semester
Basic Statistics [S Grade]School of Advanced Science and Engineering2019spring semester
Physics SeminarSchool of Advanced Science and Engineering2019fall semester
Physics Seminar [S Grade]School of Advanced Science and Engineering2019fall semester
Physical Chemistry ASchool of Advanced Science and Engineering2019fall semester
Physical Chemistry A [S Grade]School of Advanced Science and Engineering2019fall semester
Life Science and Medical Bioscience LaboratorySchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory ISchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory I [S Grade]School of Advanced Science and Engineering2019spring semester
Applied Mathematics BSchool of Advanced Science and Engineering2019summer quarter
Applied Mathematics B [S Grade]School of Advanced Science and Engineering2019summer quarter
Life Science and Medical Bio-science Seminar IISchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bio-science Seminar II [S Grade]School of Advanced Science and Engineering2019fall semester
Intermediate Life Science and Medical Bioscience LaboratorySchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bio-science Laboratory IIISchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bio-science Laboratory III [S Grade]School of Advanced Science and Engineering2019fall semester
Graduation ResearchSchool of Advanced Science and Engineering2019full year
Graduation Research [S Grade]School of Advanced Science and Engineering2019full year
Frontier of Solid State BioscienceSchool of Advanced Science and Engineering2019spring quarter
Introduction to Biomaterial AnalysisSchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory IVSchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory VSchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bioscience Seminar IISchool of Advanced Science and Engineering2019spring semester
Bioscience Practicals ASchool of Advanced Science and Engineering2019fall semester
Bioscience Practicals BSchool of Advanced Science and Engineering2019spring semester
Graduation Thesis ASchool of Advanced Science and Engineering2019fall semester
Graduation Thesis BSchool of Advanced Science and Engineering2019spring semester
Seminar on Advanced Biomedical ScienceGraduate School of Fundamental Science and Engineering2019spring semester
Seminar on Advanced Biomedical ScienceGraduate School of Creative Science and Engineering2019spring semester
Seminar on Advanced Biomedical ScienceGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Advanced Biomedical ScienceGraduate School of Advanced Science and Engineering2019spring semester
Materials Informatics αGraduate School of Fundamental Science and Engineering2019fall quarter
Materials Informatics αGraduate School of Creative Science and Engineering2019fall quarter
Materials Informatics αGraduate School of Advanced Science and Engineering2019fall quarter
Materials Informatics βGraduate School of Fundamental Science and Engineering2019winter quarter
Materials Informatics βGraduate School of Creative Science and Engineering2019winter quarter
Materials Informatics βGraduate School of Advanced Science and Engineering2019winter quarter
International Project for Advanced Science and EngineeringGraduate School of Advanced Science and Engineering2019an intensive course(spring and fall)
International Project for Advanced Science and EngineeringGraduate School of Advanced Science and Engineering2019an intensive course(spring and fall)
Master's Thesis (Department of Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2019full year
Master's Thesis (Department of Nanoscience and Nanoengineering)Graduate School of Advanced Science and Engineering2019full year
Research on Solid State BioscienceGraduate School of Advanced Science and Engineering2019full year
Research on Solid State BioscienceGraduate School of Advanced Science and Engineering2019full year
Advanced Solid State BioscienceGraduate School of Advanced Science and Engineering2019spring semester
Advanced Solid State BioscienceGraduate School of Advanced Science and Engineering2019spring semester
Advanced Solid State BioscienceGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Solid State Bioscience AGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Solid State Bioscience AGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Solid State Bioscience BGraduate School of Advanced Science and Engineering2019fall semester
Seminar on Solid State Bioscience BGraduate School of Advanced Science and Engineering2019fall semester
Research on Nano-Chiral ScienceGraduate School of Advanced Science and Engineering2019full year
Research on Nano-Chiral ScienceGraduate School of Advanced Science and Engineering2019full year
Introduction to Nano-Chiral ScienceGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Introduction to Nano-Chiral ScienceGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Experiments in Nanoscience and NanoengineeringGraduate School of Advanced Science and Engineering2019full year
Experiments in Nanoscience and NanoengineeringGraduate School of Advanced Science and Engineering2019full year
Seminar on Nano-Chiral Science CGraduate School of Advanced Science and Engineering2019spring semester
Nano-Chiral Science C :SeminarGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Nano-Chiral Science DGraduate School of Advanced Science and Engineering2019fall semester
Nano-Chiral Science D :SeminarGraduate School of Advanced Science and Engineering2019fall semester
Master's Thesis (Department of Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2019full year
Master's Thesis (Department of Nanoscience and Nanoengineering)Graduate School of Advanced Science and Engineering2019full year
Research on Solid State BioscienceGraduate School of Advanced Science and Engineering2019full year
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Science Communication and Research IntegrityGraduate School of Advanced Science and Engineering2019an intensive course(fall)
Practical Training for Career BuildingGraduate School of Advanced Science and Engineering2019full year
Research on Nano-Chiral ScienceGraduate School of Advanced Science and Engineering2019full year
Research on Nano-structured Crystal ChemistryGraduate School of Advanced Science and Engineering2019full year
Research on Life Science and Medical Bioscience ASAHI, ToruGraduate School of Advanced Science and Engineering2019full year
Research on Life Science and Medical Bioscience TAKEOKA, ShinjiGraduate School of Advanced Science and Engineering2019full year
Research on Life Science and Medical Bioscience TAKEYAMA, HarukoGraduate School of Advanced Science and Engineering2019full year
Michigan Practical EnglishGraduate School of Advanced Science and Engineering2019full year
Energy Next Problem-Solving PracticeGraduate School of Advanced Science and Engineering2019full year
Laboratory Rotation AGraduate School of Advanced Science and Engineering2019full year
Laboratory Rotation BGraduate School of Advanced Science and Engineering2019full year
Advanced Lifescience : Bio-Solid State PhysicsGraduate School of Advanced Science and Engineering2019fall quarter
Energy Next Systems and DevicesGraduate School of Advanced Science and Engineering2019an intensive course(spring and fall)
Leadership Development ProgramGraduate School of Advanced Science and Engineering2019summer quarter
Business InternshipGraduate School of Advanced Science and Engineering2019full year
Academic Research Practice and Industrial Internship AGraduate School of Advanced Science and Engineering2019full year
Academic Research Practice and Industrial Internship BGraduate School of Advanced Science and Engineering2019full year
Introduction to Energy NextGraduate School of Advanced Science and Engineering2019an intensive course(spring and fall)
Technology Policy in the European UnionGlobal Education Center2019summer
Intoroduction to Innovation alphaGlobal Education Center2019fall quarter
Intoroduction to Innovation betaGlobal Education Center2019winter quarter
Innovation and Technology Start-Up alphaGlobal Education Center2019spring quarter
Innovation and Technology Start-Up betaGlobal Education Center2019summer quarter
Innovation and Technology Practice alphaGlobal Education Center2019fall quarter
Innovation and Technology Practice betaGlobal Education Center2019winter quarter
Machine Learning for Advanced Integrated Intelligence alphaGlobal Education Center2019an intensive course(spring)
Machine Learning for Advanced Integrated Intelligence betaGlobal Education Center2019an intensive course(spring)
Machine Learning for Advanced Integrated Intelligence ganmaGlobal Education Center2019an intensive course(spring)
Introduction to Artificial IntelligenceGlobal Education Center2019an intensive course(spring)
The Application of Artificial Intelligence alpha: AgentGlobal Education Center2019an intensive course(spring)
The Application of Artificial Intelligence beta: Natural Language ProcessingGlobal Education Center2019an intensive course(spring)
Top Leader Management AGlobal Education Center2019spring quarter
Start-up Essential BGlobal Education Center2019summer quarter
Data Business Creation alphaGlobal Education Center2019fall quarter
Advanced Course on Entrepreneurship DGlobal Education Center2019winter quarter
Social Medicine From Medical Profession/Practitioners Graduated From Waseda University 1 01Global Education Center2019spring quarter
Social Medicine From Medical Profession/Practitioners Graduated From Waseda University 1 02Global Education Center2019fall quarter
Social Medicine From Medical Profession/Practitioners Graduated From Waseda University 2 01Global Education Center2019summer quarter
Social Medicine From Medical Profession/Practitioners Graduated From Waseda University 2 02Global Education Center2019winter quarter
CSR ManagementGlobal Education Center2019an intensive course(fall)
Artificial Inteligence and Business Model Creation alphaGlobal Education Center2019spring quarter
Artificial Inteligence and Business Model Creation betaGlobal Education Center2019summer quarter
Data Business Creation betaGlobal Education Center2019winter quarter
Informatoin Security in the WorkplaceGlobal Education Center2019summer quarter
Design Thinking 01Global Education Center2019summer
Design Thinking 02Global Education Center2019an intensive course(fall)
AI Business Creation alphaGlobal Education Center2019spring quarter
AI Business Creation betaGlobal Education Center2019fall quarter
Top Leader Management AGlobal Education Center2019spring quarter
Start-up Essential BGlobal Education Center2019summer quarter
Data Business Creation alphaGlobal Education Center2019fall quarter
Advanced Course on Entrepreneurship DGlobal Education Center2019winter quarter
Innovation LeadershipGlobal Education Center2019an intensive course(spring)
Innovation for Industry and Career DesignGlobal Education Center2019fall quarter
Logical CommunicationGlobal Education Center2019spring quarter
Global Business Communication FundamentalGlobal Education Center2019fall quarter
Global Business Communication AdvancedGlobal Education Center2019an intensive course(fall)
CSR ManagementGlobal Education Center2019an intensive course(fall)
Intoroduction to Innovation alphaGlobal Education Center2019fall quarter
Intoroduction to Innovation betaGlobal Education Center2019winter quarter
Innovation and Technology Start-Up alphaGlobal Education Center2019spring quarter
Innovation and Technology Start-Up betaGlobal Education Center2019summer quarter
Innovation and Technology Practice alphaGlobal Education Center2019fall quarter
Innovation and Technology Practice betaGlobal Education Center2019winter quarter
Machine Learning for Advanced Integrated Intelligence alphaGlobal Education Center2019an intensive course(spring)
Machine Learning for Advanced Integrated Intelligence betaGlobal Education Center2019an intensive course(spring)
Machine Learning for Advanced Integrated Intelligence ganmaGlobal Education Center2019an intensive course(spring)
Introduction to Artificial IntelligenceGlobal Education Center2019an intensive course(spring)
The Application of Artificial Intelligence alpha: AgentGlobal Education Center2019an intensive course(spring)
The Application of Artificial Intelligence beta: Natural Language ProcessingGlobal Education Center2019an intensive course(spring)
Data Business Creation betaGlobal Education Center2019winter quarter
Informatoin Security in the WorkplaceGlobal Education Center2019summer quarter
Design Thinking 01Global Education Center2019summer
Design Thinking 02Global Education Center2019an intensive course(fall)
AI Business Creation alphaGlobal Education Center2019spring quarter
AI Business Creation betaGlobal Education Center2019fall quarter