Name

AKANUMA, Satoshi

Official Title

Professor

Affiliation

（School of Human Sciences）

Contact Information

Mail Address

Mail Address: akanuma@waseda.jp

URL

Web Page URL

http://www.f.waseda.jp/akanuma/index.html

Grant-in-aids for Scientific Researcher Number

10321720

Sub-affiliation

Faculty of Human Sciences（e-School (Internet Degree Program), School of Human Sciences）

Faculty of Human Sciences（Graduate School of Human Sciences）

Educational background・Degree

Educational background

1989/04 -1993/03	Tokyo Institute of Technology
1995/04 -1998/03	Tokyo Institute of Technology

Degree

Ph.D.

Academic Society Joined

THE SOCIETY FOR THE STUDY OF THE ORIGIN AND EVOLUTION OF LIFE-JAPAN

THE JAPANESE BIOCHEMICAL SOCIETY

THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

JAPAN GEOSCIENCE UNION

SOCIETY OF EVOLUTIONARY STUDIES, JAPAN

The Society for Biotechnology, Japan

THE BIOPHYSICAL SOCIETY OF JAPAN

PROTEIN SCIENCE SOCIETY OF JAPAN

Award

2011 JB/OUP Poster Prize

2011/10Conferment Institution：Journal of Biochemistry/Oxford University Press

Award Winner(Group)：Akanuma Satoshi

Alexander von Humboldt Research Fellowship

2001/03Conferment Institution：Alexander von Humboldt Foundation

Award Winner(Group)：Akanuma Satoshi

Interview Guide

Category: Biology
Research Field: Evolutionary Biology, Protein Engineering
Keywords: Origin of life, Early evolution of life, Thermostable proteins

Research Field

Keywords

Protein Engineering, Molecular Evolution, Origin of Life

Grants-in-Aid for Scientific Research classification

Engineering / Process/Chemical engineering / Biofunction/Bioprocess

Paper

Creation of artificial protein-protein interactions using α-helices as interfaces.

Yagi S, Akanuma S, Yamagishi A

Biophysical reviews Peer Review Yes Invitation Yes 10(2) p.411 - 4202018/04-

ISSN：1867-2450

Selection of a platinum-binding sequence in a loop of a four-helix bundle protein.

Yagi S, Akanuma S, Kaji A, Niiro H, Akiyama H, Uchida T, Yamagishi A

Journal of bioscience and bioengineering Peer Review Yes 125(2) p.192 - 1982018/02-

ISSN：1389-1723

Activation of macrophages by a laccase-polymerized polyphenol is dependent on phosphorylation of Rac1.

Tajima K, Akanuma S, Matsumoto-Akanuma A, Yamanaka D, Ishibashi KI, Adachi Y, Ohno N

Biochemical and biophysical research communications Peer Review Yes 495(3) p.2209 - 22132018/01-

ISSN：0006-291X

Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins.

Shibue R, Sasamoto T, Shimada M, Zhang B, Yamagishi A, Akanuma S

Scientific reports Peer Review Yes 8(1) p.12272018/01-

ISSN：2045-2322

Characterization of a thermostable mutant of Agaricus brasiliensis laccase created by phylogeny-based design.

Hamuro Y, Tajima K, Matsumoto-Akanuma A, Sakamoto S, Furukawa R, Yamagishi A, Ohno N, Akanuma S

Journal of bioscience and bioengineering Peer Review Yes 124(6) p.623 - 6292017/12-

ISSN：1389-1723

Characterization of Reconstructed Ancestral Proteins Suggests a Change in Temperature of the Ancient Biosphere.

Akanuma S

Life (Basel, Switzerland) Peer Review Yes Invitation Yes 7(3) 2017/08-

人工改変による機能性タンパク質の開発と応用耐熱性酵素の創成と好熱菌酵素の低温高活性化

赤沼哲史

月刊ファインケミカル Invitation Yes 46(2) p.6‐122017/02-

ISSN：0913-6150

Reconstructed ancestral enzymes suggest long-term cooling of Earth's photic zone since the Archean.

Garcia AK, Schopf JW, Yokobori SI, Akanuma S, Yamagishi A

Proceedings of the National Academy of Sciences of the United States of America Peer Review Yes 114(18) p.4619 - 46242017/05-

ISSN：0027-8424

De novo design of protein-protein interactions through modification of inter-molecular helix-helix interface residues.

Yagi S, Akanuma S, Yamagishi M, Uchida T, Yamagishi A

Biochimica et biophysica acta Peer Review Yes 1864(5) p.479 - 4872016/05-

ISSN：0006-3002

Epistasis effects of multiple ancestral-consensus amino acid substitutions on the thermal stability of glycerol kinase from Cellulomonas sp. NT3060.

Fukuda Y, Abe A, Tamura T, Kishimoto T, Sogabe A, Akanuma S, Yokobori S, Yamagishi A, Imada K, Inagaki K

Journal of bioscience and bioengineering Peer Review Yes 121(5) p.497 - 5022016/05-

ISSN：1389-1723

A strategy for designing thermostable enzymes by reconstructing ancestral sequences possessed by ancient life

Akanuma S, Yamagishi A

Biotechnology of Extremophiles: Advances and Challenges (ed. Rampelotto PH) Peer Review Yes Invitation Yes p.581 - 5962016/04-

Publish Classification：Research paper (scientific journal)

Chapter 20: Ancestral reconstruction of enzymes

Akanuma S, Yamagishi A

Understanding enzymes; Function, Design, Engineering and Analysis (ed. Svendsen A) Invitation Yes p.683 - 7062016/04-

アストロバイオロジーとの遭遇:生命のルーツを探る旅全生物の最後の共通祖先コモノート

赤沼哲史

細胞工学 Peer Review Yes Invitation Yes 35(2) p.124 - 1282016/01-

ISSN：0287-3796

ゲノム配列の比較から明らかになった初期生命の好熱性

赤沼哲史

地球化学 Peer Review Yes Invitation Yes 50(3) p.199‐210(J‐STAGE) - 2102016/00-

ISSN：0386-4073

Birth of Archaeal Cells: Molecular Phylogenetic Analyses of G1P Dehydrogenase, G3P Dehydrogenases, and Glycerol Kinase Suggest Derived Features of Archaeal Membranes Having G1P Polar Lipids.

Yokobori SI, Nakajima Y, Akanuma S, Yamagishi A

Archaea (Vancouver, B.C.) Peer Review Yes 2016p.18026752016/00-

ISSN：1472-3646

Robustness of predictions of extremely thermally stable proteins in ancient organisms.

Akanuma S, Yokobori S, Nakajima Y, Bessho M, Yamagishi A

Evolution; international journal of organic evolution Peer Review Yes 69(11) p.2954 - 29622015/11-

ISSN：0014-3820

Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants.

Sugii T, Akanuma S, Yagi S, Yagyu K, Shimoda Y, Yamagishi A

Journal of bioscience and bioengineering Peer Review Yes 118(4) p.367 - 3712014/10-

ISSN：1389-1723

Addition of negatively charged residues can reverse the decrease in the solubility of an acidic protein caused by an artificially introduced non-polar surface patch.

Yagi S, Akanuma S, Yamagishi A

Biochimica et biophysica acta Peer Review Yes 1844(3) p.553 - 5602014/03-

ISSN：0006-3002

Cloning and characterization of laccase DNA from the Royal Sun medicinal mushroom, Agaricus brasiliensis (higher Basidiomycetes).

Matsumoto-Akanuma A, Akanuma S, Motoi M, Yamagishi A, Ohno N

International journal of medicinal mushrooms Peer Review Yes 16(4) p.375 - 3932014/00-

ISSN：1521-9437

Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose.

Takahashi H, Yamazaki H, Akanuma S, Kanahara H, Saito T, Chimuro T, Kobayashi T, Ohtani T, Yamamoto K, Sugiyama S, Kobori T

PloS one Peer Review Yes 9(2) p.e826242014/02-

ISSN：1932-6203

N-terminal hydrophobic amino acids of activating transcription factor 5 (ATF5) protein confer interleukin 1β (IL-1β)-induced stabilization.

Abe T, Kojima M, Akanuma S, Iwashita H, Yamazaki T, Okuyama R, Ichikawa K, Umemura M, Nakano H, Takahashi S, Takahashi Y

The Journal of biological chemistry Peer Review Yes 289(7) p.3888 - 39002014/02-

ISSN：0021-9258

Experimental evidence for the thermophilicity of ancestral life

Akanuma S, Nakajima Y, Yokobori S, Kimura M, Nemoto N, Mase T, Miyazono K, Tanokura M, Yamagishi A.

Proc. Natl. Acad. Sci. USA Peer Review Yes 110(27) p.11067 - 110722013/07-

ISSN：0027-8424

Evaluation of the protein interfaces that form an intermolecular four-helix bundle as studied by computer simulation

Fukuda Masaki, Komatsu Yu, Yamada Hironao, Morikawa Ryota, Miyakawa Takeshi, Takasu Masako, Akanuma Satoshi, Yamagishi Akihiko

MOLECULAR SIMULATION Peer Review Yes 40(6) p.498 - 5032014/05-

ISSN：0892-7022

全生物の共通祖先遺伝子の復元

赤沼哲史, 山岸明彦

バイオサイエンスとインダストリー Invitation Yes 72(1) p.19 - 232014/01-

ISSN：0914-8981

Exploring Sequences for Thermally Stable Proteins by the Ancestral Design Method

AKANUMA Satoshi

Seibutsu Butsuri Peer Review Yes Invitation Yes 53(3) p.128 - 1332013/05-

ISSN：5824052

Comparative Genomics of Thermophilic Bacteria and Archaea

Akanuma S, Yokobori S, Yamagishi A

Thermophilic Microbes in Environmental and Industrial Biotechnology (Eds. Satyanarayana, T., Litterchild J., Kawarabayasi, Y) Peer Review Yes Invitation Yes p.331 - 3492013/02-

Simulation study of protein-protein interfaces based on the 4-helix bundle structure

Fukuda M, Komatsu Y, Morikawa R, Miyakawa T, Takasu M, Akanuma S, Yamagishi A

AIP Conf. Proc. Peer Review Yes 1518p.606 - 6092013/00-

Publish Classification：Research paper (international conference proceedings) ISSN：0094-243X

Constructing protein nano-fiber and estimation of the electronic state around metal ions

Komatsu Yu, Fukuda Masaki, Yamada Hironao, Kawamoto Shuhei, Miyakawa Takeshi, Morikawa Ryota, Takasu Masako, Yokojima Satoshi, Akanuma Satoshi, Yamagishi Akihiko

INTERNATIONAL JOURNAL OF QUANTUM CHEMISTRY Peer Review Yes 112(24) p.3750 - 37552012/12-

ISSN：0020-7608

Designing the binding surface of proteins to construct nano-fibers

Komatsu Y, Yamada H, Kawamoto S, Fukuda M, Miyakawa T, Morikawa R, Takasu M, Akanuma S, Yamagishi A

Prog. Theor. Chem. Phys. Peer Review Yes 26p.555 - 5672012/00-

Substitutions of coenzyme-binding, non-polar residues improve the low-temperature activity of thermophilic dehydrogenases

Hayashi S, Akanuma S, Onuki W, Tokunaga C, Yamagishi A.

Biochemistry Peer Review Yes 50(40) p.8583 - 85932011/10-

ISSN：0006-2960

Phylogeny-based design of a B-subunit of DNA gyrase and its ATPase domain using a small set of homologous amino acid sequences.

Akanuma S, Iwami S, Yokoi T, Nakamura N, Watanabe H, Yokobori S, Yamagishi A.

J. Mol. Biol. Peer Review Yes 412(2) p.212 - 2252011/09-

ISSN：0022-2836

Role of N-terminus in folding of a (beta/alpha)8-barrel protein as studied by fragmentation analysis

Akanuma S, Yamagishi A.

Proteins Peer Review Yes 79(1) p.221 - 2312011/01-

ISSN：0887-3585

Partial purification and characterization of polyphenoloxidase from culinary-medicinal Royal Sun mushroom (the Himematsutake), Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae).

Matsumoto-Akanuma A, Akanuma S, Motoi M, Yamagishi A, Ohno N

International journal of medicinal mushrooms Peer Review Yes 13(1) p.73 - 822011/00-

ISSN：1521-9437

Mimicking the evolution of a thermally stable monomeric four-helix bundle by fusion of four identical single-helix peptides

Akanuma S, Matsuba T, Ueno E, Umeda N, Yamagishi A.

J. Biochem. Peer Review Yes 147(3) p.371 - 3792010/03-

ISSN：0021-924X

Why are thermophilic proteins thermally stable?

Akanuma S, Yamagishi A

Seikagaku. The Journal of Japanese Biochemical Society Peer Review Yes 81(12) p.1064 - 10712009/12-

ISSN：0037-1017

Random mutagenesis improves the low-temperature activity of the tetrameric 3-isopropylmalate dehydrogenase from the hyperthermophile Sulfolobus tokodaii.

Sasaki M, Uno M, Akanuma S, Yamagishi A.

Protein Eng. Des. Sel. Peer Review Yes 21(12) p.721 - 7272008/12-

ISSN：1741-0126

Experimental evidence for the existence of a stable half-barrel subdomain in the (beta/alpha)8-barrel fold.

Akanuma S, Yamagishi, A.

J. Mol. Biol. Peer Review Yes 382(2) p.458 - 4662008/10-

ISSN：0022-2836

Methods for reducing nonspecific interaction in antibody-antigen assay via atomic force microscopy.

Wakayama J, Sekiguchi H, Akanuma S, Ohtani T, Sugiyama S

Analytical biochemistry Peer Review Yes 380(1) p.51 - 582008/09-

ISSN：0003-2697

Identification and characterization of key substructures involved in the early folding events of a (beta/alpha)8-barrel protein as studied by experimental and computational methods.

Akanuma S, Yamagishi A

Journal of molecular biology Peer Review Yes 353(5) p.1161 - 11702005/11-

ISSN：0022-2836

A detailed unfolding pathway of a (beta/alpha)8-barrel protein as studied by molecular dynamics simulations.

Akanuma S, Miyagawa H, Kitamura K, Yamagishi A

Proteins Peer Review Yes 58(3) p.538 - 5462005/02-

ISSN：0887-3585

Combinatorial mutagenesis to restrict amino acid usage in an enzyme to a reduced set.

Akanuma S, Kigawa T, Yokoyama S

Proceedings of the National Academy of Sciences of the United States of America Peer Review Yes 99(21) p.13549 - 135532002/10-

ISSN：0027-8424

Experimental Evidence for Information Redundancy in Amino Acid Sequences: Implication for Protein Folding and Evolution.

赤沼哲史, 黒田裕

生物物理 41(5) p.224 - 2292001/10-

ISSN：0582-4052

Design, X-ray crystallography, molecular modelling and thermal stability studies of mutant enzymes at site 172 of 3-isopropylmalate dehydrogenase from Thermus thermophilus

Qu C, Akanuma S, Tanaka N, Moriyama H, Oshima T.

Acta Crystallogr D Biol Crystallogr. Peer Review Yes 57(Pt 2) p.225 - 2322001/02-

Publish Classification：Research paper (scientific journal)

Crystal structures of 3-isopropylmalate dehydrogenases with mutations at the C-terminus: crystallographic analyses of structure-stability relationships

Nurachman Z, Akanuma S, Sato T, Oshima T, Tanaka N.

Protein Eng. Peer Review Yes 13(4) p.253 - 2582000/04-

Publish Classification：Research paper (scientific journal)

Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site-directed mutagenesis.

Akanuma S, Yamagishi A, Tanaka N, Oshima T.

Eur J Biochem. Peer Review Yes 260(2) p.499 - 5041999/03-

Publish Classification：Research paper (scientific journal)

Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

Akanuma S, Yamagishi A, Tanaka N, Oshima T.

Protein Sci. Peer Review Yes 7(3) p.698 - 7051998/03-

Publish Classification：Research paper (scientific journal)

Effect of polar side chains at position 172 on thermal stability of 3-isopropylmalate dehydrogenase from Thermus thermophilus

Akanuma S, Qu C, Yamagishi A, Tanaka N, Oshima T.

FEBS Lett. Peer Review Yes 410(43134.0) p.141 - 1441997/06-

Publish Classification：Research paper (scientific journal)

A mutation at the interface between domains causes rearrangement of domains in 3-isopropylmalate dehydrogenase

Qu C, Akanuma S, Moriyama H, Tanaka N, Oshima T.

Protein Eng. Peer Review Yes 10(1) p.45 - 521997/01-

Publish Classification：Research paper (scientific journal)

Spontaneous tandem sequence duplications reverse the thermal stability of carboxyl-terminal modified 3-isopropylmalate dehydrogenase

Akanuma S, Yamagishi A, Tanaka N, Oshima T.

J Bacteriol Peer Review Yes 178(21) p.6300 - 63041996/11-

Publish Classification：Research paper (scientific journal)

Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method

Kotsuka T, Akanuma S, Tomuro M, Yamagishi A, Oshima T

J Bacteriol Peer Review Yes 178(3) p.723 - 7271996/02-

Publish Classification：Research paper (scientific journal)

Recombination-Deficient Mutants of an Extreme Thermophile, Thermus thermophilus

Takada T, Akanuma S, Kotsuka T, Tamakoshi M, Yamagishi A, Oshima T

Appl Environ Microbiol Peer Review Yes 59(8) p.2737 - 27391993/08-

Publish Classification：Research paper (scientific journal)

Lecture And Oral

Establishment of mesophilic-like catalytic property on a thermophilic enzyme without loss of thermostability

Akanuma Satoshi

The 70th Annual Meeting of the Society of Biotechnology of Japan2018/09/07

National conferenceSymposium workshop panel(nominated)

原始地球上に比較的多く存在したと推定されるアミノ酸種のタンパク質合成における役割

Akanuma Satoshi

The 20th Annual Meeting of the Society of Evolutionary Studies, Japan2018/08/22

National conferenceSymposium workshop panel(nominated)

Protein engineering studies to explore the early evolution of life

Akanuma Satoshi

The 18th Annual Meeting of the Protein Science Society of Japan2018/06/26

National conferenceSymposium workshop panel(nominated)

好熱菌酵素の常温菌酵素域へまでの低温高活性化

赤沼哲史、シュウギョウトウ、別所瑞萌、木村彦乃、山岸明彦

私立大学戦略的研究基盤形成支援事業「健康で豊かな生活を実現するスマートタンパク質工学戦略的研究拠点」最終報告会2018/03/19

National conferenceOral presentation(general)

共通祖先

Akanuma Satoshi

アストロバイオロジーセンター（ABC）シンポジウムInvitation Yes2018/01/16

National conferenceOral presentation(invited, special)

20種未満のアミノ酸によるタンパク質の再構成

Akanuma Satoshi

2017年度日本アストロバイオロジーネットワークワークショップ2017/11/17

National conferenceOral presentation(general)

初期地球環境に相対的に多く存在したアミノ酸種を用いた原始的タンパク質の構築

Akanuma Satoshi

冥王代生命学の創成・平成２９年度　白馬ワークショップ2017/10/13

National conferenceOral presentation(general)

Introduction: Dialogue between in vitro evolution and biological evolution, both of which have created new functional biomolecules

Akanuma Satoshi

The 55th Annual Meeting of the Biophysical Society of Japan2017/09/21

National conferenceSymposium workshop panel(nominated)

RNAワールドを取り巻く未解決課題－核酸合成からRNA-タンパク質ワールドへの移行まで

Akanuma Satoshi

19th Annual Meeting of the Society of Evolutionary Studies, Japan2017/08/25

National conferenceSymposium workshop panel(nominated)

初期地球環境に相対的に多く存在したアミノ酸種を用いた原始的タンパク質の構築

Akanuma Satoshi

冥王代生命学の創成・平成２９年度キックオフワークショップ2017/05/15

National conferenceOral presentation(general)

祖先型酵素改変の進化

赤沼哲史、渋江怜、張博文、羽室勇飛、笹本峻弘、別所瑞萌、横堀伸一、山岸明彦

私立大学戦略的研究基盤形成支援事業「健康で豊かな生活を実現するスマートタンパク質工学戦略的研究拠点」平成28年度年度末報告会2017/03/14

National conferenceOral presentation(general)

Thermophilicity of early life inferred by the resurrection of ancient proteins

Satoshi Akanuma

Search for life : from early Earth to exoplanetsInvitation Yes2016/12/15

International conferenceOral presentation(invited, special)

Incorporation of evolutionary information into protein design

Satoshi Akanuma, Shin-ichi Yokobori, Akihiko Yamagishi

The 39th Annual Meeting of the Molecular Biology Society of Japan2016/12/01

National conferenceSymposium workshop panel(nominated)

Creating stable proteins by ancestral design and improving the low-temperature activity of thermophilic ensymes

Satoshi Akanuma

私立大学戦略的研究基盤形成支援事業「健康で豊かな生活を実現するスマートタンパク質工学戦略的研究拠点」国際シンポジウム2016/11/29

National conferenceOral presentation(general)

Introduction: Advances in the engineering of protein oligomerization and solubility

Satoshi Akanuma

The 54th Annual Meeting of the Biophysical Society of Japan2016/11/25

National conferenceSymposium workshop panel(nominated)

学生の頃、何を考え今はやっているか･･生命の起源と私

Akanuma Satoshi

2016年生命の起原および進化学会＆日本アストロバイオロジーネットワーク夏の学校Invitation Yes2016/08/29

National conferenceOral presentation(invited, special)

Experimental evidence for thermophilic ancestral life

Satoshi Akanuma

18th Annual Meeting of the Society of Evolutionary Studies, Japan2016/08/27

National conferenceSymposium workshop panel(nominated)

Characterization of ancient proteins, life and environment by comparing extant homologous genes

Satoshi Akanuma, Shin-ichi Yokobori, Takahiro Sasamoto, Mizumo Bessho, Akihiko Yamagishi

生命の起原および進化学会第41回学術講演会2016/03/16

National conferenceOral presentation(general)

Universality and particularity of the amino acid usage of terrestrial life: Evolution of the genetic code and amino acid repertoire

Akanuma Satoshi

4th Astrobiology workshop 20162016/03/08

National conferenceOral presentation(general)

宇宙におけるタンパク質を構成するアミノ酸の種類の可能性

Akanuma Satoshi

第38回日本分子生物学会年会、第88回日本生化学会大会合同大会2015/12/04

National conferenceSymposium workshop panel(nominated)

イントロダクション：実用化を見据えた様々な生体関連分子工学の基礎および応用

Akanuma Satoshi

第38回日本分子生物学会年会、第88回日本生化学会大会合同大会2015/12/01

National conferenceSymposium workshop panel(nominated)

Reverification of the thermophilicity of early life

Satoshi Akanuma

Astrobiology Network Workshop 20152015/11/28

National conferenceOral presentation(general)

始生代遺伝子の復元と解析による始生代生物と地球環境の推定

Akanuma Satoshi

青山学院大学CATプロジェクトシンポジウムInvitation Yes2015/09/00

National conferenceOral presentation(invited, special)

Stabilization of industrially useful enzymes by the ancestral design method

Satoshi Akanuma

Novozymes Japan Biotechnology Forum 2014Invitation Yes2014/10/00

National conferenceOral presentation(invited, special)

コモノートから遡るアミノ酸の種類

Akanuma Satoshi

第16回日本進化学会大会2014/08/00

National conferenceSymposium workshop panel(nominated)

古代タンパク質の復元と解析および超好熱性タンパク質設計への応用

赤沼哲史，山岸明彦

第14回日本蛋白質科学会2014/06/00

National conferenceSymposium workshop panel(public)

進化情報に基づく酵素の耐熱化

Akanuma Satoshi

第15回酵素応用シンポジウムInvitation Yes2014/06/00

National conferenceOral presentation(invited, special)

進化情報に基づく古代タンパク質の復元とタンパク質設計への応用

Akanuma Satoshi

第23回内毒素・LPS研究会Invitation Yes2014/06/00

National conferenceOral presentation(invited, special)

Experimentally testing the hypothesis of a limited amino acid repertoire in primitive proteins

Satoshi Akanuma

International Astrobiology Workshop 2013Invitation Yes2013/11/00

National conferenceOral presentation(invited, special)

遺伝暗号の起源

Akanuma Satoshi

総研大「惑星科学と生命科学の融合」第3回研究会Invitation Yes2013/08/00

National conferenceOral presentation(invited, special)

Research Grants & Projects

Grant-in-aids for Scientific Research Adoption Situation

Research Classification：

Establishment of an innovative technology to develop artificial protein fibers

2016/-0-2019/-0

Allocation Class：￥3640000

Research Classification：

Establishment of a guideline to improve the low-temperature activity of thermophilic enzymes

2012/-0-2015/-0

Allocation Class：￥5330000

Research Classification：

Analysis of the proteins possessed by the last common ancestor and the organisms present before it

2011/-1-2014/-0

Allocation Class：￥10140000

Research Classification：

Study on the common ancestors of Archaea, Bacteria and Commonote based on the resurrected enzymes.

Allocation Class：￥20540000

On-campus Research System

Special Research Project

進化情報に基づく好熱菌酵素の効率的低温高活性化法の確立

2015

Research Results Outline：様々な条件で安定な好熱菌酵素は多くの工業用途が期待されているが、低温で触媒活性が著しく低下することが利用の妨げとなっている。そこで、相同アミノ酸配列の様々な条件で安定な好熱菌酵素は多くの工業用途が期待されているが、低温で触媒活性が著しく低下することが利用の妨げとなっている。そこで、相同アミノ酸配列の比較に基づく好熱菌酵素の低温高活性化法の確立に取り組んできた。本研究では、好熱菌酵素と常温菌相同酵...様々な条件で安定な好熱菌酵素は多くの工業用途が期待されているが、低温で触媒活性が著しく低下することが利用の妨げとなっている。そこで、相同アミノ酸配列の比較に基づく好熱菌酵素の低温高活性化法の確立に取り組んできた。本研究では、好熱菌酵素と常温菌相同酵素とでアミノ酸が異なる部位のうち、活性部位に近い範囲に低温高活性化に関わるアミノ酸が多くある可能性と、耐熱性の犠牲無しに低温活性を改善できる可能性を検証した。その結果、活性部位からの距離と低温活性化に寄与するアミノ酸置換が見つかる頻度には相関がないことと、耐熱性をほとんど低下させることなく25℃の比活性を10倍以上向上できることを明らかにした。

好熱菌酵素の効率的低温高活性化法の開発

2015

Research Results Outline：好熱菌酵素の工業利用の推進には、高い安定性を維持したまま、低温活性を向上させるための改変法の開発が課題である。この目的のため、好熱菌と大腸菌酵素のアミ好熱菌酵素の工業利用の推進には、高い安定性を維持したまま、低温活性を向上させるための改変法の開発が課題である。この目的のため、好熱菌と大腸菌酵素のアミノ酸配列を比較し、両者で異なるアミノ酸のうち、活性部位に近いアミノ酸を大腸菌型に置換することによっ...好熱菌酵素の工業利用の推進には、高い安定性を維持したまま、低温活性を向上させるための改変法の開発が課題である。この目的のため、好熱菌と大腸菌酵素のアミノ酸配列を比較し、両者で異なるアミノ酸のうち、活性部位に近いアミノ酸を大腸菌型に置換することによって、効率的に好熱菌酵素の低温活性を改善できるか検証した。計29変異体を作製し、それらの25℃における比活性を測定したところ、10変異体が好熱菌野生型酵素と比べて２倍以上大きい活性を示した。さらに、有益なアミノ酸置換を組み合わせた結果、最大で野生型酵素の１４倍の比活性を持つ変異体が得られた。加えて、この変異体の変性温度は85℃と高い耐熱性を維持していた。

進化情報を利用した担子菌由来ポリフェノール重合化酵素の安定化改変

2016

Research Results Outline：Laccase is a kind of polyphenol oxidases and frequentlyfound among higher pLaccase is a kind of polyphenol oxidases and frequentlyfound among higher plants and fungi. We previously cloned a laccase-c...Laccase is a kind of polyphenol oxidases and frequentlyfound among higher plants and fungi. We previously cloned a laccase-coding genefrom a cDNA library of the Royal Sun medicinal mushroom, Agaricus brasiliensis. However, our efforts to yield the wild-typelaccase was unsuccessful probably due to its instability. Therefore, in thisstudy, we tried to improve the stability of the laccase by the ancestral designmethod. The designed laccase was successfully yielded by a Pichia pastoris secretoryexpression system and then purified. Thermal stability measurements of thepurified laccase showed that the protein is nicely thermostable; its half-lifeat 70ºC is almost 70 min. The half-inactivationtemperature of the protein after heat-treatment for 10 min is higher than 80ºC. The kinetic properties are reasonably similarto those of a laccase from a closely related species, A. bisporus. We also found that the designed laccase can catalyze the polymerization of severalmonomeric polyphenols.

耐熱性と低温活性を両立した酵素の創成

2017

Research Results Outline：好熱菌酵素は優れた耐熱性を持つが、低温では活性が大幅に低下する。これまでの研究では、高度好熱菌Thermus thermophilus由来3-イソプロ好熱菌酵素は優れた耐熱性を持つが、低温では活性が大幅に低下する。これまでの研究では、高度好熱菌Thermus thermophilus由来3-イソプロピルリンゴ酸脱水素酵素（IPMDH）のアミノ酸配列と、大腸菌由来IPMDHのアミノ酸配列を比較し、...好熱菌酵素は優れた耐熱性を持つが、低温では活性が大幅に低下する。これまでの研究では、高度好熱菌Thermus thermophilus由来3-イソプロピルリンゴ酸脱水素酵素（IPMDH）のアミノ酸配列と、大腸菌由来IPMDHのアミノ酸配列を比較し、両者でアミノ酸が異なる部位に、常温菌IPMDHに見られるアミノ酸を好熱菌IPMDHにアミノ酸置換により導入した変異型酵素を作製した。今年度は、すでに作製された変異型酵素の詳細な触媒活性の解析をおこなった。その結果、わずか３アミノ酸置換によって、好熱菌酵素の高い耐熱性を損ねることなく、低温活性を常温菌酵素と同程度にまで改善できることを明らかにした。

祖先タンパク質再構成による初期地球生物圏の環境pHの推定

2018

Research Results Outline：地球上で最初の生命が誕生した前後の初期地球における生物生息圏の環境pHついては、いくつかの議論があるものの、当時の地質学的情報が少ないため、未だに明確地球上で最初の生命が誕生した前後の初期地球における生物生息圏の環境pHついては、いくつかの議論があるものの、当時の地質学的情報が少ないため、未だに明確な知見は得られていない。本研究では、進化系統解析と遺伝子工学的手法によって復元した、全生物の共通祖...地球上で最初の生命が誕生した前後の初期地球における生物生息圏の環境pHついては、いくつかの議論があるものの、当時の地質学的情報が少ないため、未だに明確な知見は得られていない。本研究では、進化系統解析と遺伝子工学的手法によって復元した、全生物の共通祖先が持っていたと推測されるタンパク質の安定性のpH依存性を測定した。復元した全生物の共通祖先タンパク質は中性で最も安定であり、酸性とアルカリ性では安定性が大きく低下した。同様の傾向が、中性環境に生息する現存生物の複数のタンパク質でも見られた。以上の結果から、全生物の共通祖先は中性環境に生息していたと推定された。

原始アミノ酸だけによるRNA結合タンパク質の構築

2019Collaborator：古川龍太郎, 趙方正

Research Results Outline：生命の起源の解明に向けてRNAワールド仮説が支持を広げているが、次の課題となるのがRNAワールドにどのようにタンパク質が誕生したかである。本研究では、生命の起源の解明に向けてRNAワールド仮説が支持を広げているが、次の課題となるのがRNAワールドにどのようにタンパク質が誕生したかである。本研究では、RNA結合タンパク質の代表例として、RNA機能に対して補助的ではあるが必須の役割を担っているリボソ...生命の起源の解明に向けてRNAワールド仮説が支持を広げているが、次の課題となるのがRNAワールドにどのようにタンパク質が誕生したかである。本研究では、RNA結合タンパク質の代表例として、RNA機能に対して補助的ではあるが必須の役割を担っているリボソームタンパク質S7とS8を研究材料として選択し、原始地球環境に比較的豊富に存在したと推定されるアミノ酸種だけからS7とS8を再構成する試みを進めている。現在までに、進化系統解析と人工遺伝子合成により共通祖先型S7とS8を復元した。さらに、祖先型S7とS8から特定のアミノ酸種を欠損させたアミノ酸組成単純化型改変体の再構成もおこなっている。

Lecture Course

Course Title	School	Year	Term
Basic Seminar I	School of Human Sciences	2020	spring semester
Basic Seminar I	School of Human Sciences	2020	spring semester
Introduction to Human Behavior and Environment Sciences	School of Human Sciences	2020	spring quarter
Practical Training in Environmental Bioscience and Chemistry	School of Human Sciences	2020	winter quarter
Environmental Microbiology	School of Human Sciences	2020	spring semester
Seminar I (Life Sciences for Extremophilest)	School of Human Sciences	2020	spring semester
Seminar II (Life Sciences for Extremophilest)	School of Human Sciences	2020	fall semester
Introduction to Human Behavior and Environment Sciences	School of Human Sciences (Online Degree Program)	2020	fall quarter
Environmental Microbiology	School of Human Sciences (Online Degree Program)	2020	fall semester
Life Sciences for Extremophiles A	Graduate School of Human Sciences	2020	spring semester
Life Sciences for Extremophiles B	Graduate School of Human Sciences	2020	fall semester
Life Sciences for Extremophiles (1) A	Graduate School of Human Sciences	2020	spring semester
Life Sciences for Extremophiles (1) B	Graduate School of Human Sciences	2020	fall semester
Life Sciences for Extremophiles (1) C	Graduate School of Human Sciences	2020	spring semester
Life Sciences for Extremophiles (1) D	Graduate School of Human Sciences	2020	fall semester
Life Sciences for Extremophiles (2) A	Graduate School of Human Sciences	2020	spring semester
Life Sciences for Extremophiles (2) B	Graduate School of Human Sciences	2020	fall semester
Life Sciences for Extremophiles (2) C	Graduate School of Human Sciences	2020	spring semester
Life Sciences for Extremophiles (2) D	Graduate School of Human Sciences	2020	fall semester
Practical Training in Environmental Biology	Graduate School of Human Sciences	2020	an intensive course(fall)
Advanced Course of Life Sciences for Extremophiles	Graduate School of Human Sciences	2020	fall quarter
Life Sciences for Extremophiles(D) A	Graduate School of Human Sciences	2020	spring semester
Life Sciences for Extremophiles(D) B	Graduate School of Human Sciences	2020	fall semester

Waseda Course Channel Video Service

Course Title	Faculty	Publication Year
Environmental Microbiology	School of Human Sciences (Correspondence Course)	2016
Environmental Microbiology 02	School of Human Sciences (Correspondence Course)	2016