Name

TSUNEDA, Satoshi

Official Title

Professor

Affiliation

(School of Advanced Science and Engineering)

Contact Information

Mail Address

Mail Address
stsuneda@waseda.jp

Address・Phone Number・Fax Number

Address
2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Phone Number
+81-3-5369-7325
Fax Number
+81-3-5369-7325

URL

Web Page URL

http://www.waseda.jp/sem-tsuneda/index.html

Grant-in-aids for Scientific Researcher Number
30281645

Sub-affiliation

Sub-affiliation

Faculty of Science and Engineering(Graduate School of Advanced Science and Engineering)

Research Council (Research Organization)/Affiliated organization(Global Education Center)

Affiliated Institutes

理工学総合研究センター

兼任研究員 1989-2006

ライフサポートイノベーション研究所

研究所員 2014-2014

実践的ナノ化学G

研究所員 2007-2011

欧州バイオメディカルサイエンス研究所

研究所員 2012-2012

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2012-2014

持続型食・農・バイオ研究所

研究所員 2014-

ナノプロセス研究所

研究所員 2007-2014

ナノプロセス研究所

研究所員 2015-

ライフサポートイノベーション研究所

研究所員 2015-

リンアトラス研究所

プロジェクト研究所所長 2015-

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2015-2016

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2017-2017

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2017-

理工学術院総合研究所(理工学研究所)

兼任研究員 2006-2018

理工学術院総合研究所(理工学研究所)

兼任研究員 2018-

Educational background・Degree

Educational background

-1989 The University of Tokyo Faculty of Engineering Department of Chemical Engineering
-1991 The University of Tokyo Graduate School, Division of Engineering Department of Chemical Engineering
-1994 The University of Tokyo Graduate School, Division of Engineering Department of Chemistry and Biotechnology

Degree

Doctor of Engineering Coursework The University of Tokyo Biofunction/Bioprocess

Career

1995-1996The Institute of Physical and Chemical Research, Special Researcher
1996-1999Waseda University, Assistant Professor
1999-Waseda University, Associate Professor
2007-Waseda University, Professor
2012-2014Waseda University, Environmenta Safety Center, Direcotr

Research Field

Keywords

Bioengineering, Microbial Ecology

Grants-in-Aid for Scientific Research classification

Engineering / Process/Chemical engineering / Biofunction/Bioprocess

Environmental science / Environmental conservation / Environmental engineering and reduction of environmental burden

Agricultural sciences / Agricultural chemistry / Applied microbiology

Medicine, dentistry, and pharmacy / Basic medicine / Bacteriology (including mycology)

Research interests Career

2010-Molecular Analysis of Persister Cells in Biofilms

Current Research Theme Keywords:Biofilm, Persister cells, Molecular mechanism

Cooperative Research within Japan

2008-Modulating Intestinal Inflammatory Response by Commensal Bacteria

Current Research Theme Keywords:Intestinal microbiota, inflammation, Intestine epithelial cell

Cooperative Research within Japan

2003-Development of New Cultivating System for Uncultured Microorganisms

Current Research Theme Keywords:uncultured microorganism, micro-colony, cell sorter

Individual research allowance

2006-Quantitative Method and Molecular Imaging Technique of Gene Expression for Medical Diagnostics

Cooperative Research within Japan

1992-New Surface Design for Immobilization of Biomolecules

Current Research Theme Keywords:Biomolecule, Surface

Individual research allowance

1996-Biological Treatment of Industrial and Domestic Wastewater

Current Research Theme Keywords:Biological treatment, Industrial wastewater, Domestic wastewater

Individual research allowance

1998-Elucidation of Microbial Ecology in Biofilms

Current Research Theme Keywords:Biofilm, Microbial ecology, Persister cell

Individual research allowance

Paper

Nitrosomonas europaea MazF Specifically Recognises the UGG Motif and Promotes Selective RNA Degradation

T. Miyamoto, A. Yokota, Y. Ota, M. Tsuruga, R. Aoi, S. Tsuneda, N. Noda

Frontiers in Microbiology Peer Review Yes 9(2386) p.1 - 102018/10-2018/10

DOI

Detail

Publish Classification:Research paper (scientific journal)

Stochastic expression of lactate dehydrogenase A induces Escherichia coli persister formation

N. Yamamoto, R. Isshiki, Y. Kawai, D. Tanaka, T. Sekiguchi, S. Matsumoto, S. Tsuneda

J. Biosci. Bioeng. Peer Review Yes 126(1) p.30 - 372018/07-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Low-temperature Culturing Improves Survival Rate of Tissue-engineered Cardiac Cell Sheets

K. Sakaguchi, Y. Hinata, Y.Kagawa, K. Iwasaki, S. Tsuneda, T. Shimizu, M. Umezu

Biochem. Biophys. Rep. Peer Review Yes 14p.89 - 972018/07-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Skewed megakaryopoiesis in human induced pluripotent stem cell-derived haematopoietic progenitor cells harbouring calreticulin mutations

H. Takei, Y. Edahiro, S. Mano, N. Masubuchi, Y. Mizukami, M. Imai, S. Morishita, S. Tsuneda, H. Endo, S. Nakamura, K. Eto, A. Ohsaka, M. Araki, N. Komatsu

British Journal of Haematology Peer Review Yes 181(6) p.791 - 8022018/06-

DOI

Detail

Publish Classification:Research paper (scientific journal)

AldB controls persister formation in Escherichia coli depending on environmental stress

Y. Kawai, S. Matsumoto, Y. Ling, S. Okuda, S. Tsuneda

Microbiol. Immunol. Peer Review Yes 62(5) p.299 - 3042018/05-

DOI

Detail

Publish Classification:Research paper (scientific journal)

“All-in-one” in vitro selection of collagen-binding vascular endothelial growth factor

S.-H. Park, T. Uzawa, F. Hattori, S. Ogino, N. Morimoto, S. Tsuneda, Y. Ito

Biomaterials Peer Review Yes 161p.270 - 2782018/02-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Genomic Analysis of Two Phylogenetically Distinct Nitrospira Species Reveals Their Genomic Plasticity and Functional Diversity

N. Ushiki, H. Fujitani, Y. Shimada, T. Morohoshi, Y. Sekiguchi, S. Tsuneda

Frontiers in Microbiology Peer Review Yes 8(2637) p.1 - 122018/01-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Unique Transcriptional Profile of Native Persisters in Escherichia coli

S. Matsumoto, Y. Kawai, S. Miyagawa, Y. Iwamoto, S. Okuda, A. Sanchez-Gorostiaga, M. Vicente, S. Tsuneda

J. Biosci. Bioeng. Peer Review Yes 125(1) p.15 - 222018/01-

DOI

Detail

Publish Classification:Research paper (scientific journal)

High-Rate Nitrification of Electronic Industry Wastewater by Using Nitrifying Granules

Y. Hasebe, H. Meguro, Y. Kanai, M. Eguchi, T. Osaka, S. Tsuneda

Water Sci. Technol. Peer Review Yes 76(11) p.3171 - 31802017/12-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Characterization of a Deinococcus radiodurans MazF: a UACA-Specific RNA Endoribonuclease

T. Miyamoto, Y. Ota, A. Yokota T. Suyama, S. Tsuneda, N. Noda

MicrobiologyOpen Peer Review Yes 6(5) p.e5012017/12-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Meta-analysis of Fecal Microbiota and Metabolites in Experimental Colitic Mice during the Inflammatory and Healing Phases

T. Osaka, E. Moriyama, S. Arai, Y. Date, J. Yagi, J. Kikuchi, S. Tsuneda

Nutrients Peer Review Yes 9(12) p.13292017/12-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Enrichment and Physiological Characterization of a Cold-Adapted Nitrite Oxidizer Nitrotoga sp. from Eelgrass Sediment

K. Ishii, H. Fujitani, K. Soh, T. Nakagawa, R. Takahashi, S. Tsuneda

Appl. Environ. Microbiol. Peer Review Yes 83(14) p.e00549-172017/07-

DOI

Detail

Publish Classification:Research paper (scientific journal)

First Full-Scale Nitritation-Anammox Plant Using Gel Entrapment Technology for Ammonia Plant Effluent

K. Isaka, Y. Kimura, M. Matsuura, T. Osaka, S. Tsuneda

Biochem. Eng. J. Peer Review Yes 122p.115 - 1222017/06-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Real-Time Quantitation of Internal Metabolic Activity of Three-Dimensional Engineered Tissues Using an Oxygen Microelectrode and Optical Coherence Tomography

Y. Kagawa, Y. Haraguchi, S. Tsuneda, T. Shimizu

Journal of Biomedical Materials Research: Part B Peer Review Yes 105(4) p.855 - 8642017/05-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Nitrite Oxidation Kinetics of Two Nitrospira Strains: the Quest for Competition and Ecological Niche Differentiation

N. Ushiki, M. Jinno, H. Fujitani, T. Suenaga, A. Terada, S. Tsuneda

J. Biosci. Bioeng. Peer Review Yes 123(5) p.581 - 5892017/05-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Four Aromatic Sulfates with an Inhibitory Effect against HCV NS3 Helicase from the Crinoid Alloeocomatella polycladia

I. Hermawan, A. Furuta, M. Higashi, Y. Fujita, N. Akimitsu, A. Yamashita, K. Moriishi, S. Tsuneda, H. Tani, M. Nakakoshi, M. Tsubuki, Y. Sekiguchi, N. Noda, J. Tanaka

Marine Drugs Peer Review Yes 15(117) p.1 - 102017/05-

DOI

Detail

Publish Classification:Research paper (scientific journal)

pH-Gradient Ion-exchange Microbial Cell Chromatography as a Simple Method for Microbial Separation

Y. Aoi, Y. Kaneko, S. Tsuneda

J. Biosci. Bioeng. Peer Review Yes 123(4) p.431 - 4362017/04-

DOI

Detail

Publish Classification:Research paper (scientific journal)

A rapid collection of yet unknown ammonia oxidizers in pure culture from activated sludge

Abe, Takuma; Ushiki, Norisuke; Fujitani, Hirotsugu; Tsuneda, Satoshi

Water Research Peer Review Yes 108(1) p.169 - 1782017/01-2017/01

DOIScopus

Detail

Publish Classification:Research paper (scientific journal) ISSN:00431354

Outline:© 2016 Elsevier LtdNitrification is an important reaction in the biological nitrogen removal process in wastewater treatment plants (WWTPs). As ammonia-oxidizing microbes are slow-growing and sensitive to environmental factors such as free ammonia, pure strains are hard to obtain, preventing our understanding of their physiological characteristics. To conquer this hurdle, we report a high-throughput isolation technique based on scattering signatures, which exploits the tendency of many ammonia-oxidizing bacteria (AOB) to form microcolonies in activated sludge. The AOB microcolonies were directly sorted from the activated sludge without long incubation and enrichment bias, and were sequentially inoculated into 96-well microtiter plates containing growth medium. Phylogenetic analysis of the pure strains isolated in this study revealed a deeply branching and unrecognized lineage and diversity within the genus Nitrosomonas, beyond our expectation.

Ecophysiology and Comparative Genomics of Nitrosomonas mobilis Ms1 Isolated from Autotrophic Nitrifying Granules of Wastewater Treatment Bioreactor

S. M. Thandar, N. Ushiki, H. Fujitani, Y. Sekiguchi, S. Tsuneda

Frontiers in Microbiology Peer Review Yes 7(1869) p.1 - 142016/11-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Physical enrichment of uncultured Accumulibacter and Nitrospira from activated sludge by unlabeled cell sorting technique

Irie, Kana; Fujitani, Hirotsugu; Tsuneda, Satoshi

Journal of Bioscience and Bioengineering Peer Review Yes 122(4) p.475 - 4812016/10-2016/10

DOIScopus

Detail

Publish Classification:Research paper (scientific journal) ISSN:13891723

Outline:© 2016 The Society for Biotechnology, JapanIt is important to understand the ecology and physiology of microbes in activated sludge of wastewater treatment plants. Recently, molecular based approaches such as 16S rRNA genes and environmental genomics have illuminated black boxes in nutrient removal process and expanded our knowledge. However, most microbes responsible for the removal of phosphate and nitrogen such as Accumulibacter and Nitrospira remain uncultured. This is because optimum methodologies to concentrate these uncultured microbes and to obtain pure cultures have not been established. Here, we report a novel approach for physical enrichment of uncultured Accumulibacter and Nitrospira from microbial communities in activated sludge by a cell sorting system. Two scattering signatures representing forward scatter and side scatter of this system allowed morphological characterization of microbial particles in activated sludge. The distribution and size of microbial particles consisting of single cells, microcolonies, and aggregates depended on the levels of scattering signatures. Next generation sequencer and principal component analysis revealed each microbial population fractionated according to the levels of scattering signatures, resulting that uncultured Accumulibacter and Nitrospira could be sorted as single cells or microcolonies. Finally, quantitative fluorescence in situ hybridization analysis determined optimum fractions to collect sufficiently these target microbes from activated sludge. Consequently, this method would be very useful as an enrichment technique prior to isolation, genomic analysis, and physiological investigation of uncultured bacteria.

AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea

Miyamoto, Tatsuki; Miyamoto, Tatsuki; Yokota, Akiko; Tsuneda, Satoshi; Noda, Naohiro; Noda, Naohiro

Toxins Peer Review Yes 8(6) p.n° 1742016/06-2016/06

DOIScopus

Detail

Publish Classification:Research paper (scientific journal)

Outline:© 2016 by the authors; licensee MDPI, Basel, Switzerland.Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.

Effect of Lipopolysaccharide on the Progression of Non-Alcoholic Fatty Liver Disease in High Caloric Diet-Fed Mice

Matsushita, N.; Matsushita, N.; Osaka, T.; Osaka, T.; Haruta, I.; Haruta, I.; Ueshiba, H.; Yanagisawa, N.; Omori-Miyake, M.; Hashimoto, E.; Shibata, N.; Tokushige, K.; Saito, K.; Tsuneda, S.; Yagi, J.

Scandinavian Journal of Immunology Peer Review Yes 83(2) p.109 - 1182016/02-2016/02

PubMedDOIScopus

Detail

Publish Classification:Research paper (scientific journal) ISSN:03009475

Outline:© 2016 The Foundation for the Scandinavian Journal of Immunology.The incidence of non-alcoholic steatohepatitis (NASH) is increasing. Because gut microbiota have been highlighted as one of the key factors in the pathogenesis of metabolic syndrome, we investigated the involvement of the bacterial component in the progression of non-alcoholic fatty liver (NAFL) to NASH. C57BL/6 mice were fed with maintenance food (MF, groups A and B) or a high caloric diet (HCD, groups C and D) for 1 month. Mice were then divided into four groups: Groups A and C were inoculated with PBS, while groups B and D were inoculated with lipopolysaccharide (LPS) plus complete Freund's adjuvant (CFA). The inoculations were performed a total of 3 times over 3 months. At 6 months, while hepatic steatosis was observed in groups C and D, cellular infiltration and fibrosis were less evident in group C than in group D. Inflammatory cytokines were upregulated in groups B and D. 16S rRNA pyrosequencing of whole colon homogenates containing faeces showed that certain bacterial groups, such as Bacteroidaceae, Peptostreptococcaceae and Erysipelotrichaceae, were increased in groups C and D. Although loading of bacterial components (LPS) resulted in hepatic inflammation in both MF- and HCD-fed mice, HCD feeding was more crucial in the progression of NAFL during the triggering phase.

Characterization of mazF-mediated sequence-specific RNA cleavage in pseudomonas putida using massive parallel sequencing

Miyamoto, Tatsuki; Miyamoto, Tatsuki; Kato, Yuka; Sekiguchi, Yuji; Tsuneda, Satoshi; Noda, Naohiro; Noda, Naohiro

PLoS ONE 11(2) p.e01494942016/02-2016/02

DOIScopus

Detail

Publish Classification:Research paper (scientific journal)

Outline:© 2016 Miyamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Under environmental stress, microbes are known to alter their translation patterns using sequence-specific endoribonucleases that we call RNA interferases. However, there has been limited insight regarding which RNAs are specifically cleaved by these RNA interferases, hence their physiological functions remain unknown. In the current study, we developed a novel method to effectively identify cleavage specificities with massive parallel sequencing. This approach uses artificially designed RNAs composed of diverse sequences, which do not form extensive secondary structures, and it correctly identified the cleavage sequence of a well-characterized Escherichia coli RNA interferase, MazF, as ACA. In addition, we also determined that an uncharacterized MazF homologue isolated from Pseudomonas putida specifically recognizes the unique triplet, UAC. Using a real-time fluorescence resonance energy transfer assay, the UAC triplet was further proved to be essential for cleavage in P. putida MazF. These results highlight an effective method to determine cleavage specificity of RNA interferases.

System for measuring oxygen consumption rates of mammalian cells in static culture under hypoxic conditions

Kagawa, Yuki; Kagawa, Yuki; Miyahara, Hirotaka; Ota, Yuri; Tsuneda, Satoshi; Tsuneda, Satoshi

Biotechnology Progress Peer Review Yes 32(1) p.189 - 1972016/01-2016/01

DOIScopus

Detail

Publish Classification:Research paper (scientific journal) ISSN:87567938

Outline:© 2016 American Institute of Chemical Engineers.Estimating the oxygen consumption rates (OCRs) of mammalian cells in hypoxic environments is essential for designing and developing a three-dimensional (3-D) cell culture system. However, OCR measurements under hypoxic conditions are infrequently reported in the literature. Here, we developed a system for measuring OCRs at low oxygen levels. The system injects nitrogen gas into the environment and measures the oxygen concentration by an optical oxygen microsensor that consumes no oxygen. The developed system was applied to HepG2 cells in static culture. Specifically, we measured the spatial profiles of the local dissolved oxygen concentration in the medium, then estimated the OCRs of the cells. The OCRs, and also the pericellular oxygen concentrations, decreased nonlinearly as the oxygen partial pressure in the environment decreased from 19% to 1%. The OCRs also depended on the culture period and the matrix used for coating the dish surface. Using this system, we can precisely estimate the OCRs of various cell types under environments that mimic 3-D culture conditions, contributing crucial data for an efficient 3-D culture system design.

Bacterial metabolites directly modulate farnesoid X receptor activity

Zhang, Xianqin; Osaka, Toshifumi; Osaka, Toshifumi; Tsuneda, Satoshi

Nutrition and Metabolism Peer Review Yes 12(1) p.1 - 142015/11-2015/11

DOIScopus

Detail

Publish Classification:Research paper (scientific journal)

Outline:© 2015 Zhang et al.Background: The farnesoid X receptor (FXR), a ligand-activated transcription factor belonging to the adopted orphan receptor, plays an important role in maintaining health of the liver and intestine. In this study, we identified individual bacterial strains that directly modulated the activation of intestinal FXR. Methods: The FXR stimulatory potential of 38 bacterial strains was determined using a stable FXR reporter system derived from intestinal epithelial cells (IEC). The induction of FXR target genes by screened FXR stimulatory bacteria was determined by real-time PCR. In addition, a high fat diet (HFD)-induced obese mouse model was used to evaluate in vivo FXR stimulatory potential of bacterial metabolites screened in this study. Results: A luciferase assay with the FXR reporter cell line demonstrated that the FXR-stimulatory activity of most bacterial cell samples was less than 2-fold. The culture supernatants of Bacteroides dorei and Eubacterium limosum induced FXR activity and selectively regulated FXR target expression in the FXR reporter system. Treatment with B. dorei-derived metabolites strongly induced ileal bile acid binding protein (IBABP) (8.4-fold) and organic solute transporter (OST) α (3.1-fold) compared with E. limosum-derived metabolites. Furthermore, administration of B. dorei derived metabolites showed significant reduction in body weight gain, and both two bacterial metabolites reduced liver weight in obese mice compared to PBS-treated controls. Administration of each bacterial metabolites improved in serum levels of obesity-related metabolic biochemical markers such as ALT, AST, total cholesterol, and triglyceride. Furthermore, two bacterial metabolites enhanced the Fxr gene expression in the intestine and liver, and ileal Shp gene expression tended to be increased by treatment with the metabolites derived from B. dorei. Conclusions: B. dorei and E. limosum secreted the bioactive substances that directly stimulate FXR in the intestinal epithelial cells. Administration of these bacterial FXR-stimulatory metabolites improves the obesity phenotype including body weight gain, liver damage, lipid metabolism in DIO mice.

Selective Isolation of Ammonia-Oxidizing Bacteria from Autotrophic Nitrifying Granules by Applying Cell-Sorting and Sub-Culturing of Microcolonies

H. Fujitani, A. Kumagai, N. Ushiki, K. Momiuchi, S. Tsuneda

Frontiers in Microbiology Peer Review Yes 6(1159) p.1 - 102015/10-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Identification of hydroxyanthraquinones as novel inhibitors of hepatitis C Virus NS3 helicase

Furuta, Atsushi; Furuta, Atsushi; Tsubuki, Masayoshi; Endoh, Miduki; Miyamoto, Tatsuki; Miyamoto, Tatsuki; Tanaka, Junichi; Salam, Kazi Abdus; Akimitsu, Nobuyoshi; Tani, Hidenori; Yamashita, Atsuya; Moriishi, Kohji; Nakakoshi, Masamichi; Sekiguchi, Yuji; Tsuneda, Satoshi; Noda, Naohiro; Noda, Naohiro

International Journal of Molecular Sciences Peer Review Yes 16(8) p.18439 - 184532015/08-

DOIScopus

Detail

Publish Classification:Research paper (scientific journal) ISSN:16616596

Outline:© 2015 by the authors; licensee MDPI, Basel, Switzerland. Hepatitis C virus (HCV) is an important etiological agent of severe liver diseases, including cirrhosis and hepatocellular carcinoma. The HCV genome encodes nonstructural protein 3 (NS3) helicase, which is a potential anti-HCV drug target because its enzymatic activity is essential for viral replication. Some anthracyclines are known to be NS3 helicase inhibitors and have a hydroxyanthraquinone moiety in their structures; mitoxantrone, a hydroxyanthraquinone analogue, is also known to inhibit NS3 helicase. Therefore, we hypothesized that the hydroxyanthraquinone moiety alone could also inhibit NS3 helicase. Here, we performed a structure–activity relationship study on a series of hydroxyanthraquinones by using a fluorescence-based helicase assay. Hydroxyanthraquinones inhibited NS3 helicase with IC50 values in the micromolar range. The inhibitory activity varied depending on the number and position of the phenolic hydroxyl groups, and among different hydroxyanthraquinones examined, 1,4,5,8-tetrahydroxyanthraquinone strongly inhibited NS3 helicase with an IC50 value of 6 μM. Furthermore, hypericin and sennidin A, which both have two hydroxyanthraquinone-like moieties, were found to exert even stronger inhibition with IC50 values of 3 and 0.8 μM, respectively. These results indicate that the hydroxyanthraquinone moiety can inhibit NS3 helicase and suggest that several key chemical structures are important for the inhibition.

Direct Measurement of Local Dissolved Oxygen Concentration Spatial Profiles in a Cell Culture Environment

Y. Kagawa, K. Matsuura, T. Shimizu, S. Tsuneda

BIOTECHNOLOGY AND BIOENGINEERING Peer Review Yes 112(6) p.1263 - 12742015/06-

DOIWoS

Detail

Publish Classification:Research paper (scientific journal) ISSN:0006-3592

Mathematical modeling of dormant cell formation in growing biofilm

K. Chihara, S. Matsumoto, Y. Kagawa, S. Tsuneda

FRONTIERS IN MICROBIOLOGY Peer Review Yes 6(534) p.1 - 82015/05-

DOIWoS

Detail

ISSN:1664-302X

Phosphorylated 5-ethynyl-2′-deoxyuridine for advanced DNA labeling

Seo, Siyoong; Seo, Siyoong; Onizuka, Kazumitsu; Nishioka, Chieko; Takahashi, Eiki; Tsuneda, Satoshi; Abe, Hiroshi; Abe, Hiroshi; Ito, Yoshihiro; Ito, Yoshihiro; Ito, Yoshihiro

Organic and Biomolecular Chemistry Peer Review Yes 13(15) p.4589 - 45952015/04-2015/04

DOIScopus

Detail

Publish Classification:Research paper (scientific journal) ISSN:14770520

Outline:© 2015 The Royal Society of Chemistry. The representative DNA-labeling agent 5-ethynyl-2′-deoxyuridine (EdU) was chemically modified to improve its function. Chemical monophosphorylation was expected to enhance the efficiency of the substrate in DNA polymerization by circumventing the enzymatic monophosphorylation step that consumes energy. In addition, to enhance cell permeability, the phosphates were protected with bis-pivaloyloxymethyl that is stable in buffer and plasma, and degradable inside various cell types. The phosphorylated EdU (PEdU) was less toxic than EdU, and had the same or a slightly higher DNA-labeling ability in vitro. PEdU was also successfully applied to DNA labeling in vivo. In conclusion, PEdU can be used as a less toxic DNA-labeling agent for studies that require long-term cell survival or very sensitive cell lines.

Melting Curve Analysis after T Allele Enrichment (MelcaTle) as a Highly Sensitive and Reliable Method for Detection of JAK2V617F Mutation

S. Morishita, K. Takahashi, M. Araki, Y. Hironaka, Y. Sunami, Y. Edahiro, M.Tsutsui, A. Ohsaka, S. Tsuneda, N. Komatsu

PLoS ONE Peer Review Yes 10(3) p.e01220032015/03-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Strengthening of the Intestinal Epithelial Tight Junction by Bifidobacterium bifidum

Physiological Reports Peer Review Yes 3(3) p.e123272015/03-

DOI

Detail

Publish Classification:Research paper (scientific journal)

Modeling the Nutrient Removal Process in Aerobic Granular Sludge System by Coupling the Reactor- and Granule-Scale Models

Y. Kagawa, J. Tahata, N. Kishida, S. Matsumoto, C. Picioreanu, M.C.M. van Loosdrecht, S. Tsuneda

Biotechnol. Bioeng. 112(1) p.53 - 642015/01-

DOI

Isolation of Sublineage I Nitrospira by a Novel Cultivation Strategy

H. Fujitani, N. Ushiki, S. Tsuneda, Y. Aoi

Environ. Microbiol. 16(10) p.3030 - 30402014/10-

DOI

Detection of MPLW515L/K Mutations and Determination of Allele Frequencies with a Single-Tube PCR Assay

H. Takei, S. Morishita, M. Araki, Y. Edahiro, Y. Sunami, Y. Hironaka, N. Noda, Y. Sekiguchi, S. Tsuneda, A. Ohsaka, N. Komatsu

PLoS ONE 9(8) p.e1049582014/08-

DOI

Mathematical Modeling of Spatio-Temporal Cellular Dynamics in Colonic Crypts Following Irradiation

T. Murano, Y. Kagawa, S. Tsuneda

Cell Proliferation 47(4) p.347 - 3552014/08-

DOI

Phosphorylated Gelatin to Enhance Cell Adhesion to Titanium

S.-H. Park, L. Zhu, S. Tada, S. Obuse, Y. Yoshida, M. Nakamura, T.I. Son, S. Tsuneda, Y. Ito

Polymer International 63p.1616 - 16192014/07-

DOI

JAK2V617F Mutation Status and Allele Burden in Classical Ph-Negative Myeloproliferative Neoplasms in Japan

Y. Edahiro, S. Morishita, K. Takahashi, Y. Hironaka, Y. Yahata, Y. Sunami, S. Shirane, M. Tsutsui, M. Noguchi, M. Koike, K. Imai, K. Kirito, N. Noda, Y. Sekiguchi, S. Tsuneda, A. Ohsaka, M. Araki, N. Komatsu

Int. J. Hematology 99(5) p.625 - 6342014/05-

DOI

PBDE: Structure-Activity Studies for the Inhibition of Hepatitis C Virus NS3 Helicase

K. A. Salam, A. Furuta, N. Noda, S. Tsuneda, Y. Sekiguchi, A. Yamashita, K. Moriishi, M. Nakakoshi, H. Tani, J. Tanaka, M. Tsubuki, N. Akimitsu

Molecules 19(4) p.4006 - 40202014/04-

DOI

Cholesterol Sulfate as a Potential Inhibitor of Hepatitis C Virus NS3 Helicase

A. Furuta, K. A. Salam, N. Akimitsu, J. Tanaka, H. Tani, A. Yamashita, K. Moriishi, M. Nakakoshi, M. Tsubuki, Y. Sekiguchi, S. Tsuneda, N. Noda

J. Enzym. Inhib. Med. Chem. 29(2) p.223 - 2292014/02-

DOI

Modeling of Stem Cell Dynamics in Human Colonic Crypts in silico

Y. Kagawa, N. Horita, H. Taniguchi, S. Tsuneda

Journal of Gastroenterology 49p.263 - 2692014/02-

DOI

Identification and Biochemical Characterization of Halisulfate 3 and Suvanine as Novel Inhibitors of Hepatitis C Virus NS3 Helicase from a Marine Sponge

A. Furuta, K. A. Salam, I. Hermawan, N. Akimitsu, J. Tanaka, H. Tani, A. Yamashita, K. Moriishi, M. Nakakoshi, M. Tsubuki, P. W. Peng, Y. Suzuki, N. Yamamoto, Y. Sekiguchi, S. Tsuneda, N. Noda

Marine Drugs 12p.462 - 4762014/01-

DOI

Applicability of a Sequencing Batch Membrane Biofilm Reactor for Simultaneous Nitrogen and Phosphorus Removal from Low C/N Ratio Wastewater

A. Terada, J. ito, S. Matsumoto, S. Tsuneda

J. Water Environ. Technol. 11(6) p.488 - 4962013/12-

DOI

Psammaplin A Inhibits Hepatitis C Virus NS3 Helicase

K. A. Salam, A. Furuta, N. Noda, S. Tsuneda, Y. Sekiguchi, A. Yamashita, K. Moriishi, M. Nakakoshi, M. Tsubuki, H. Tani, J. Tanaka, N. Akimitsu

J. Nat. Med. 67(4) p.765 - 7722013/09-

DOI

Isolation of Nitrospira Belonging to Sublineage II from a Wastewater Treatment Plant

N. Ushiki, H. Fujitani, Y. Aoi, S. Tsuneda

Microbes and Environments 28(3) p.346 - 3532013/09-

DOI

Complete Autotrophic Denitrification in a Single Reactor Using Nitritation and Anammox Gel Carriers

K. Isaka, Y. Kimura, T. Yamamoto, T. Osaka, S. Tsuneda

Bioresource Technol. 147p.96 - 1012013/08-

DOI

Selective Enrichment of Two Different Types Nitrospira-like Nitrite-Oxidizing Bacteria from a Wastewater Treatment Plant

H. Fujitani, Y. Aoi, S. Tsuneda

Microbes and Environments 28(2) p.236 - 2432013/06-

DOI

Detection of Pre-mRNA Splicing in vitro by an RNA-Templated Fluorogenic Reaction

Y. Tamura, K. Furukawa, R. Yoshimoto, Y. Kawai, M. Yoshida, S. Tsuneda, Y. Ito, H. Abe

Bioorganic & Medicinal Chemistry Letters 22(23) p.7248 - 72512012/12-

DOI

複合微生物系の挙動を表現可能な数理モデル

松本慎也,加川友己,常田聡

化学工学 76(11) p.671 - 6732012/11-

Inhibition of Both Protease and Helicase Activities of Hepatitis C Virus NS3 by an Ethyl Acetate Extract of Marine Sponge Amphimedon sp.

Y. Fujimoto, K. A. Salam, A. Furuta, Y. Matsuda, O. Fujita, H. Tani, M. Ikeda, N. Kato, N. Sakamoto, S. Maekawa, N. Enomoto, N. J. de Voogd, M. Nakakoshi, M. Tsubuki, Y. Sekiguchi, S. Tsuneda, N. Akimitsu, N. Noda, A. Yamashita, J. Tanaka, K. Moriishi

PLoS One 7(11) p.e486852012/11-

DOI

High-Rate Denitrification Using Polyethylene Glycol Gel Carriers Entrapping Heterotrophic Denitrifying Bacteria

K. Isaka, Y. Kimura, T. Osaka, S. Tsuneda

Water Res. 46(16) p.4941 - 49482012/11-

DOI

Temperature Dependence for Anammox Bacteria Enriched from Freshwater Sediments

T. Osaka, Y. Kimura, Y. Otsubo, Y. Suwa, S. Tsuneda, K. Isaka

J. Biosci. Bioeng. 114(4) p.429 - 4342012/10-

DOI

The Effect of Surface Charge Property on Escherichia coli Initial Adhesion and Subsequent Biofilm Formation

A. Terada, K. Okuyama, M. Nishikawa, S. Tsuneda, M. Hosomi

Biotechnol. Bioeng. 109(7) p.1745 - 17542012/07-

DOI

還元反応を引き金とするoff/on型蛍光プローブを用いたRNA検出法の開発

阿部洋,柴田綾,古川和寛,常田聡,伊藤嘉浩

化学と生物 50(7) p.540 - 5442012/07-

Challenge for Formation of Aerobic Granular Sludge in a Continuous-Flow Reactor

N. Kishida, R. Totsuka, S. Tsuneda

J. Water Environ. Technol. 10(2) p.79 - 862012/06-

DOI

Inhibition of Hepatitis C Virus NS3 Helicase by Manoalide

K. A. Salam, A. Furuta, N. Noda, S. Tsuneda, Y. Sekiguchi, A. Yamashita, K. Moriishi, M. Nakakoshi, M. Tsubuki, H. Tani, J. Tanaka, N. Akimitsu

J. Nat. Prod. 75(4) p.650 - 6542012/04-

DOI

システム論的アプローチによる複合微生物系の解析と制御

松本慎也,常田 聡

生物工学 90(4) p.160 - 1642012/04-

Inhibition of Hepatitis C Virus Replication and Viral Helicase by Ethyl Acetate Extract of the Marine Feather Star Alloeocomatella polycladia

A. Yamashita, K. A. Salam, A. Furuta, Y. Matsuda, O. Fujita, H. Tani, Y. Fujita, Y. Fujimoto, M. Ikeda, N. Kato, N. Sakamoto, S. Maekawa, N. Enomoto, M. Nakakoshi, M. Tsubuki, Y. Sekiguchi, S. Tsuneda, N. Akimitsu, N. Noda, J. Tanaka, K. Moriishi

Marine Drugs 10p.744 - 7612012/03-

DOI

Formation of Nitrifying Granules Using a Continuous Stirred Tank Reactor

N. Kishida, Y. Yamashita, S. Tsuneda

J. Water Environ. Technol. 10(1) p.47 - 552012/03-

DOI

Rapid Start-up of a Nitrifying Reactor Using Aerobic Granular Sludge as Seed Sludge

N. Kishida, G. Saeki, S. Tsuneda, R. Sudo

Water Sci. Technol. 65(3) p.581 - 5872012/01-

DOI

Quantitative Detection of Cryptosporidium Oocyst in Water Source Based on 18S rRNA by Alternately Binding Probe Competitive Reverse Transcription Polymerase Chain Reaction (ABC-RT-PCR)

N. Kishida, R. Miyata, A. Furuta, S. Izumiyama, S. Tsuneda, Y. Sekiguchi, N. Noda, M. Akiba

Water Res. 46(1) p.187 - 1942012/01-

DOI

Fluorescent Detection of Intron Lariat RNAs with Reduction-Triggered Fluorescence Probes

K. Furukawa, H. Abe, Y. Tamura, R. Yoshimoto, M. Yoshida, S. Tsuneda, Y. Ito

Angew. Chem. Int. Ed. 50(50) p.12020 - 120232011/12-

DOI

Synthesis, Structure, and Biological Activity of Dumbbell-Shaped Nanocircular RNAs for RNA Interference

N. Abe, H. Abe, C. Nagai, M. Harada, H. Hatakeyama, H. Harashima, T. Ohshiro, M. Nishihara, K. Furukawa, M. Maeda, S. Tsuneda, Y. Ito

Bioconjugate Chem. 22(10) p.2082 - 20922011/12-

DOI

Alternately Binding Probe Competitive PCR as a Simple, Cost-Effective, and Accurate Quantification Method for JAK2V617F Allele Burden in Myeloproliferative Neoplasms

S. Morishita, N. Komatsu, K. Kirito, A. H. Koda, Y. Sekiguchi, S. Tsuneda, N. Noda

Leukemia Res. 35(12) p.1632 - 16362011/12-

DOI

Significance of Reactor Type for High-Rate Nitrification Using Aerobic Granular Sludge

Y. Hasebe, M. Eguchi, H. Meguro, S. Tsuneda

J. Water Environ. Technol. 9(3) p.289 - 2962011/09-

DOI

Reproducible Subcutaneous Transplantation of Cell Sheets into Recipient Mice

H. Obokata, M. Yamato, S. Tsuneda, T. Okano

Nature Protocols 6(7) p.1053 - 10592011/06-

DOI

包括固定化担体を用いた脱窒システムの開発

井坂和一,安部直樹,木村裕哉,渡部雅智,大坂利文,常田聡

日本水処理生物学会誌 47(2) p.67 - 742011/06-

Enhancing Nitrogen and Phosphorus Removal in an Anaerobic/Oxic/Anoxic Sequencing Batch Reactor: The Dependence of the Amount of External Carbon

K. Soejima, A. Terada, K. Naraki, S. Tsuneda

J. Water Environ. Technol. 9(1) p.79 - 862011/03-

DOI

The Potential of Stem Cells in Adult Tissues Representative of the Three Germ Layers

H. Obokata, K. Kojima, K. Westerman, M. Yamato, T. Okano, S. Tsuneda, C.A. Vacanti

Tissue Eng. Part A 17(5-6) p.607 - 6152011/02-

DOI

ゼオライろ床と植栽を組み合わせた里川再生技術の実河川への適用と維持管理

木持謙,金澤光,真下敏明,正田武則,常田聡,関根正人,榊原豊

用水と廃水 53(2) p.141 - 1492011/02-

Application of Nitrifying Granules to Improvement of Nitrification Activity in Activated Sludge Process

N. Kishida, R. Totsuka, A. Kono, M. Kurasawa, M. Ogiwara, S. Tsuneda

Int. J. Environ. Waste Management 7(1-2) p.122 - 1302011/01-

DOI

Behavior of Polymeric Substrates in an Aerobic Granular Sludge System

M. de Kreuk, N. Kishida, S. Tsuneda, M.C.M. van Loosdrecht

Water Res. 44(20) p.5929 - 59382010/12-

DOI

Formation of Aerobic Granular Sludge in a Continuous Flow Reactor - Control Strategy for Selection of Well-Settling Granular Sludge -

N. Kishida, A. Kono, Y. Yamashita, S. Tsuneda

J. Water Environ. Technol. 8(3) p.251 - 2582010/09-

DOI

Microbial Population Dynamics and Community Structure during Formation of Nitrifying Granule Treating Ammonia-Rich Inorganic Wastewater

S. Matsumoto, D. Ishikawa, G. Saeki, Y. Aoi, S. Tsuneda

Microbes and Environments 25(3) p.164 - 1702010/09-

DOI

Direct Cloning and Expression of Putative Esterase Genes from Environmental DNA

T. Terahara, K. Yamada, S. Kurata, T. Yokomaku, S. Tsuneda, S. Harayama

Enzyme Microb. Technol. 47(1-2) p.17 - 232010/07-

DOI

New Monitoring Approach for Metabolic Dynamics in Microbial Ecosystems Using Stable-Isotope-Labeling Technologies

Y. Date, Y. Nakanishi, S. Fukuda, T. Kato, S. Tsuneda, H. Ohno, J. Kikuchi

J. Biosci. Bioeng. 110(1) p.87 - 932010/07-

DOI

Quantitative detection of chloroethene-reductive bacteria Dehalococcoides spp. using alternately binding probe competitive polymerase chain reaction

Miyata, Ryo;Adachi, Ken;Tani, Hidenori;Kurata, Shinya;Nakamura, Kazunori;Tsuneda, Satoshi;Sekiguchi, Yuji;Noda, Naohiro

MOLECULAR AND CELLULAR PROBES 24(3) p.131 - 1372010-2010

DOIWoS

Detail

ISSN:0890-8508

Photoactivatable fluorescein derivatives with azidomethyl caging groups for tracing oligonucleotides in living human cells

Furukawa, Kazuhiro;Abe, Hiroshi;Tsuneda, Satoshi;Ito, Yoshihiro

ORGANIC & BIOMOLECULAR CHEMISTRY 8(10) p.2309 - 23112010-2010

DOIWoS

Detail

ISSN:1477-0520

単一槽型窒素除去を志向したバイオフィルムリアクターの進展

寺田昭彦,常田聡

水環境学会誌 33(4) p.114 - 1202010/04-

好気性グラニュールを用いた新しい水処理技術

岸田直裕,常田聡

月刊浄化槽 406p.14 - 182010/02-

Real-time monitoring of RNA helicase activity using fluorescence resonance energy transfer in vitro

Tani, Hidenori;Fujita, Osamu;Furuta, Atsushi;Matsuda, Yasuyoshi;Miyata, Ryo;Akimitsu, Nobuyoshi;Tanaka, Junichi;Tsuneda, Satoshi;Sekiguchi, Yuji;Noda, Naohiro

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 393(1) p.131 - 1362010-2010

DOIWoS

Detail

ISSN:0006-291X

Transitions in Oral and Intestinal Microflora Composition and Innate Immune Receptor-Dependent Stimulation during Mouse Development

M. Hasegawa, T. Osaka, K. Tawaratsumida, T. Yamazaki, H. Tada, R. Mu?oz-Planillo, S. Tsuneda, G. Nu?ez, N. Inohara

Infect. Immun. 78(2) p.639 - 6502010/02-

Microbial Community Structure in Autotrophic Nitrifying Granules Characterized by Experimental and Simulation Analyses

S. Matsumoto, M. Katoku, G. Saeki, A. Terada, Y. Aoi, S. Tsuneda, C. Picioreanu, M.C.M. van Loosdrecht

Environ. Microbiol. 12(1) p.192 - 2062009/12-

Reduction-Triggered Fluorescence Probe for Peptide-Templated Reactions

A. Shibata, H. Abe, K. Furukawa, S. Tsuneda, Y. Ito

Chem. Pharm. Bull. 57(11) p.1223 - 12262009/11-

Characterization of the Microbial Community in the Anaerobic/Oxic/Anoxic Process Combined with Sludge Ozonation and Phosphorus Adsorption

T. Kondo, S. Tsuneda, Y. Ebie, Y. Inamori, K. Xu

J. Water Environ. Technol. 7(3) p.155 - 1622009/09-

Fibrous Support Stabilizes Nitrification Performance of a Membrane-Aerated Biofilm: The Effect of Liquid Flow Perturbation

A. Terada, J. Ito, S. Matsumoto, S. Tsuneda

J. Chem. Eng. Jpn. 42(8) p.607 - 6152009/08-

Universal Quenching Probe System: Flexible, Specific, and Cost-Effective Real-Time PCR Method

H. Tani, R. Miyata, K. Ichikawa, S. Morishita, S. Kurata, K. Nakamura, S. Tsuneda, Y. Sekiguchi, N. Noda

Anal. Chem. 81(14) p.5678 - 56852009/07-

Affinity Capillary Electrophoresis with Magnetic Beads for Multiplex Quantitative Analysis of Bacterial 16S rRNA

K. Adachi, M. Yamaguchi, M. Nakashige, T. Kanagawa, M. Torimura, S. Tsuneda, Y. Sekiguchi, N. Noda

J. Biosci. Bioeng. 107(6) p.662 - 6672009/06-

Hollow Fiber Membrane Chamber as a Device for in situ Environmental Cultivation

Y. Aoi, T. Kinoshita, T. Hata, H. Ohta, H. Obokata, S. Tsuneda

Appl. Environ. Microbiol. 75(11) p.3826 - 38332009/06-

Improvement of Nutrient Removal and Phosphorus Recovery in the Anaerobic/Oxic/Anoxic Process Combined with Sludge Ozonation and Phosphorus Adsorption

T. Kondo, S. Tsuneda, Y. Ebie, Y. Inamori, K. Xu

J. Water Environ. Technol. 7(2) p.135 - 1422009/06-

Reduction-Triggered Fluorescent Amplification Probe for Detection of Endogenous RNAs in Living Human Cells

K. Furukawa, H. Abe, K. Hibino, Y. Sako, S. Tsuneda, Y. Ito

Bioconjugate Chem. 20(5) p.1026 - 10362009/05-

新規分離培養手法の開発とその意義

青井議輝,常田聡

生物工学 87(4) p.179 - 1822009/04-

Reduction-Triggered Red Fluorescent Probes for Dual-Color Detection of Oligonucleotide Sequences

K. Furukawa, H. Abe, J. Wang, M. Uda, H. Koshino, S. Tsuneda, Y. Ito

Org. Biomol. Chem. 7(4) p.671 - 6772009/04-

Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid and Simple Quantification of WT1 mRNA

S. Morishita, H. Tani, S. Kurata, K. Nakamura, S. Tsuneda, Y. Sekiguchi, N. Noda

Clin. Biochem. 42(6) p.515 - 5202009/04-

Molecular Diversity of Bacterial Chitinases in Arable Soils and the Effects of Environmental Factors on the Chitinolytic Bacterial Community

T. Terahara, S. Ikeda, C. Noritake, K. Minamisawa, K. Ando, S. Tsuneda, S. Harayama

Soil Biol. Biochem. 41(3) p.473 - 4802009/03-

Microbial Diversity of Anammox Bacteria Enriched from Different Types of Seed Sludge in an Anaerobic Continuous-Feeding Cultivation Reactor

Y. Date, K. Isaka, H. Ikuta, T. Sumino, N. Kaneko, S. Yoshie, S. Tsuneda, Y. Inamori

J. Biosci. Bioeng. 107(3) p.281 - 2862009/03-

Simultaneous Nitrogen and Phosphorus Removal from High-Strength Industrial Wastewater Using Aerobic Granular Sludge

N. Kishida, S. Tsuneda, J.H. Kim, R. Sudo

J. Environ. Eng. 135(3) p.153 - 1582009/03-

High-Throughput Screening Assay of Hepatitis C Virus Helicase Inhibitors Using Fluorescence-Quenching Phenomenon

H. Tani, N. Akimitsu, O. Fujita, Y. Matsuda, R. Miyata, S. Tsuneda, M. Igarashi, Y. Sekiguchi, N. Noda

Biochem. Biophys. Res. Comm. 379(4) p.1054 - 10592009/02-

Anaerobic Ammonium Oxidation (Anammox) Irreversibly Inhibited by Methanol

K. Isaka, Y. Suwa, Y. Kimura, T. Yamagishi, T. Sumino, S. Tsuneda

Appl. Microbiol. Biotechnol. 81(2) p.379 - 3852008/11-

Microbial Community of Anammox Bacteria Immobilized in Polyethylene Glycol Gel Carrier

Y. Date, K. Isaka, T. Sumino, S. Tsuneda, Y. Inamori

Water Sci. Technol. 58(5) p.1121 - 11282008/10-

Subcutaneous Transplantation of Autologous Oral Mucosal Epithelial Cell Sheets Fabricated on Temperature-Responsive Culture Dishes

H. Obokata, M. Yamato, K. Nishida, S. Tsuneda. T. Okano

J. Biomed. Mater. Res. A 86A(4) p.1088 - 10962008/09-

Tertiary Treatment of Domestic Wastewater Using Zeolite Ceramics and Aquatic Plants

Y. Kimochi, T. Masada, Y. Mikami, S. Tsuneda, R. Sudo

Water Sci. Technol. 58(4) p.847 - 8512008/09-

Real-Time Control Strategy for Simultaneous Nitrogen and Phosphorus Removal Using Aerobic Granular Sludge

N. Kishida, S. Tsuneda, Y. Sakakibara, J.H. Kim, R. Sudo

Water Sci. Technol. 58(2) p.445 - 4502008/08-

Fluorescence Generation from Tandem Repeats of a Malachite Green RNA Aptamer Using Rolling Circle Transcription

K. Furukawa, H. Abe, N. Abe, M. Harada, S. Tsuneda, Y. Ito

Bioorganic & Medicinal Chemistry Letters 18(16) p.4562 - 45652008/08-

Effects of Carbon Source on Denitrification Efficiency and Microbial Community Structure in a Saline Wastewater Treatment Process

T. Osaka, K. Shirotani, S. Yoshie, S. Tsuneda

Water Res. 42(14) p.3709 - 37182008/08-

ダンベル型ナノサークルRNAでRNA干渉を長期安定に

阿部洋,阿部奈保子,原田充,常田聡,伊藤嘉浩

バイオサイエンスとインダストリー 66(8) p.453 - 4552008/08-

水処理プロセスにおけるリン資源の除去・廃棄から回収・資源化へのパラダイムシフト

蛯江美孝,近藤貴志,徐 開欽,常田 聡,杉浦則夫,丸山 治,稲森 悠平

ケミカルエンジニヤリング 53(7) p.530 - 5352008/07-

A Reduction-Triggered Fluorescence Probe for Sensing Nucleic Acids

H. Abe, J. Wang, K. Furukawa, K. Oki, M. Uda, S. Tsuneda, Y. Ito

Bioconjugate Chem. 19(6) p.1219 - 12262008/06-

細胞内遺伝子シグナルの解析

阿部洋,古川和寛,常田聡,伊藤嘉浩

生物工学 86(5) p.268 - 2702008/06-

Modeling and Experimental Study on the Anaerobic/Aerobic/Anoxic Process for Simultaneous Nitrogen and Phosphorus Removal: The Effect of Acetate Addition

K. Soejima, S. Matsumoto, S. Ogushi, K. Naraki, A. Terada, S. Tsuneda, A. Hirata

Process Biochem. 43(6) p.605 - 6142008/06-

DNA Microarray Mediated Transcriptional Profiling of Nitrosomonas europaea in Response to Linear Alkylbenzene Sulfonates

H. Urakawa, J. Matsumoto, K. Inaba, S. Tsuneda

FEMS Microbiol. Lett. 282(2) p.166 - 1732008/05-

Identification of the Bacterial Community Involved in Methane-Dependent Denitrification in Activated Sludge Using DNA Stable-Isotope Probing

T. Osaka, Y. Ebie, S. Tsuneda, Y Inamori

FEMS Microbiol. Ecology 64(3) p.494 - 5062008/05-

Nitrogen Removal Performance Using Anaerobic Ammonium Oxidation at Low Temperatures

K. Isaka, Y. Date, Y. Kimura, T. Sumino, S. Tsuneda

FEMS Microbiol. Lett. 282(1) p.32 - 382008/05-

Retrieval of Entire Genes from Environmental DNA by Inverse PCR with Pre-Amplification of Target Genes Using Primers Containing Locked Nucleic Acids

K. Yamada, T. Terahara, S. Kurata, T. Yokomaku, S. Tsuneda, S. Harayama

Environ. Microbiol. 10(4) p.978 - 9872008/04-

Rhodamine-Based Fluorogenic Probe for Imaging Biological Thiol

A. Shibata, K. Furukawa, H. Abe, S. Tsuneda, Y. Ito

Bioorganic & Medicinal Chemistry Letters 18(7) p.2246 - 22492008/04-

Novel Nitritation Process Using Heat-Shocked Nitrifying Bacteria Entrapped in Gel Carrier

K. Isaka, T. Sumino, S. Tsuneda

Process Biochem. 43(3) p.265 - 2702008/03-

微生物固定化のための膜の改質と水処理分野への適用

常田聡,寺田昭彦

33(2) p.54 - 622008/03-

Estimation of Single-Nucleotide Polymorphism Allele Frequency by Alternately Binding Probecompetitive Polymerase Chain Reaction (ABC-PCR)

H. Tani, N. Morita, S. Kurata, K. Nakamura, T. Kanagawa, S. Tsuneda, Y. Sekiguchi, N. Noda

Anal. Chim. Acta 608(2) p.211 - 2162008/02-

Low Temperature Decreases Phylogenetic Diversity of Ammonia-Oxidizing Archaea and Bacteria in Aquarium Biofiltration Systems

H. Urakawa, Y. Tajima, Y. Numata, S. Tsuneda

Appl. Environ. Microbiol. 74(3) p.894 - 9002008/02-

Rapid DNA Chemical Ligation for Amplification of RNA and DNA Signal

H. Abe, Y. Kondo, H. Jinmei, N. Abe, K. Furukawa, A. Uchiyama, S. Tsuneda, K. Aikawa, I. Matsumoto, Y. Ito

Bioconjugate Chem. 19(1) p.327 - 3332008/01-

Psg18 is specifically expressed in follicle-associated epithelium

K. Kawano, M. Ebisawa, K. Hase, S. Fukuda, A. Hijikata, S. Kawano, Y. Date, S. Tsuneda, K. Itoh, H. Ohno

Cell Struct. Funct. 32(2) p.115 - 1262007/12-

Ammonium Removal Performance of Anaerobic Ammonium-Oxidizing Bacteria Immobilized in Polyethylene Glycol Gel Carrier

K. Isaka, Y. Date, T. Sumino, S. Tsuneda

Appl. Microbiol. Biotechnol. 76(6) p.1457 - 14652007/10-

Modeling of Membrane-Aerated Biofilm for Feasibility Study of Simultaneous Nitrification and Denitrification

S. Matsumoto, A. Terada, S. Tsuneda

Biochem. Eng. J. 37(1) p.98 - 1072007/10-

Nitrification of Landfill Leachate Using Immobilized Nitrifying Bacteria at Low Temperatures

K. Isaka, S. Yoshie, T. Sumino, Y. Inamori, S. Tsuneda

Biochem. Eng. J. 37(1) p.49 - 552007/10-

リン回収技術の現状と将来展望

蛯江美孝,近藤貴志,徐開欽,常田聡,稲森悠平

再生と利用 30(117) p.6 - 102007/10-

細胞内遺伝子発現検出用の蛍光バイオプローブの設計と合成

阿部洋,古川和寛,常田聡,伊藤嘉浩

蛋白質拡散酵素 52(13) p.1619 - 16242007/10-

Analysis of the Relationship between Ammonia Oxidizing Bacterial Populations and Nitrification Efficiency in Full-Scale Advanced Johkasou Using Different Structured Carriers by Real-Time PCR

G. Nakagawa, Y. Ebie, S. Tsuneda, M. Matsumura, Y. Inamori

Jpn. J. Water Treatment Biol. 43(3) p.143 - 1492007/09-

Calibration-Curve-Free Quantitative PCR: A Quantitative Method for Specific Nucleic Acid Sequences without Using Calibration Curves

H. Tani, T. Kanagawa, N. Morita, S. Kurata, K. Nakamura, S. Tsuneda, N. Noda

Anal. Biochem. 369(1) p.105 - 1112007/09-

Diversity of Nitrite Reductase Genes in Accumulibacter phosphatis Dominant Cultures Enriched by Flow-Cytometric Sorting

R. Miyauchi, K. Oki, Y. Aoi, S. Tsuneda

Appl. Environ. Microbiol. 73(16) p.5331 - 53372007/08-

Technique for Quantitative Detection of Specific DNA Sequences Using Alternately Binding Quenching Probe Competitive Assay Combined with Loop-Mediated Isothermal Amplification

H. Tani, T. Teramura, K. Adachi, S. Tsuneda, S. Kurata, K. Nakamura, T. Kanagawa, N. Noda

Anal. Chem. 79(15) p.5608 - 56132007/08-

Detection of Defluvicoccus-Relative Glycogen-Accumulating Organisms in Enhanced Biological Phosphorus Removal Processes

T. Kondo, Y. Ebie, S. Tsuneda, Y. Inamori

Microbes and Environments 22(2) p.190 - 1952007/06-

Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

H. Urakawa, J. Matsumoto, T. Hoshino, S. Tsuneda

Microbes and Environments 22(2) p.116 - 1222007/06-

Experimental and Simulation Analysis of Community Structure of Nitrifying Bacteria in a Membrane-Aerated Biofilm

S. Matsumoto, A. Terada, Y. Aoi, S. Tsuneda, E. Alpkvist, C. Picioreanu, M.C.M. van Loosdrecht

Water Sci. Technol. 55(8,9) p.283 - 2902007/05-

High Nitrogen Removal Performance at Moderately Low Temperature Utilizing Anaerobic Ammonium Oxidation Reactions

K. Isaka, T. Sumino, S. Tsuneda

J. Biosci. Bioeng. 103(5) p.486 - 4902007/05-

RNA Microarray for Estimating Relative Abundance of 16S rRNA in Microbial Communities

T. Hoshino, K. Furukawa, S. Tsuneda, Y. Inamori

J. Microb. Methods 69(2) p.406 - 4102007/05-

Redox-Stratification Controlled Biofilm (ReSCoBi) for Completely Autotrophic Nitrogen Removal: The Effect of Co- versus Counter-Diffusion on Reactor Performance

A. Terada, S. Lackner, S. Tsuneda, B. F. Smets

Biotechnol. Bioeng. 97(1) p.40 - 512007/05-

シミュレーションによるバイオフィルムシステム解析の進展

松本慎也,青井議輝,常田聡

ケミカルエンジニヤリング 52(5) p.359 - 3632007/05-

Use of Real-Time PCR to Examine the Relationship between Ammonia Oxidizing Bacterial Populations and Nitrogen Removal Efficiency in a Small Decentralized Treatment System "Johkasou"

G. Nakagawa, Y. Ebie, S. Tsuneda, M. Matsumura, Y. Inamori

Water Sci. Technol. 55(7) p.203 - 2102007/04-

ゼオライト成形体の導入による高機能窒素除去型の植栽水質浄化法の開発

木持謙,正田武則,三上恭弘,常田聡

月刊「水」 May-49(700) p.27 - 322007/04-

Characterization of the High-Density Bacteria in Biological Phosphorus Removal Process by Using Buoyant Density Separation

T. Kondo, Y. Ebie, S. Tsuneda, Y. Inamori

Jpn. J. Water Treatment Biol. 43(1) p.51 - 622007/03-

Determination of Polyphosphate-Accumulating Organisms Activity in Enhanced Biological Phosphorus Removal Using Conventional Methods and Microautoradiograly-FISH

T. Kondo, Y. Ebie, S. Tsuneda, Y. Inamori

Jpn. J. Water Treatment Biol. 43(1) p.19 - 292007/03-

Quantitative Method for Specific Nucleic Acid Sequences Using Competitive Polymerase Chain Reaction with an Alternately Binding Probe

H. Tani, T. Kanagawa, S. Kurata, T. Teramura, K. Nakamura, S. Tsuneda, N. Noda

Anal. Chem. 79(3) p.974 - 9792007/02-

Bacterial Adhesion to and Viability on Positively Charged Polymer Surfaces

A. Terada, A. Yuasa, T. Kushimoto, S. Tsuneda, A. Katakai, M. Tamada

Microbiology 152(12) p.3573 - 35832006/12-

Comprehensive Analysis of Cell Wall Permeabilizing Conditions for Highly Sensitive Fluorescence In Situ Hybridization

K. Furukawa, T. Hoshino, S. Tsuneda, Y. Inamori

Microbes and Environments 21(4) p.227 - 2342006/12-

Effects of Acetate and Nitrite Addition on Fraction of Denitrifying Phosphate-Accumulating Organisms and Nutrient Removal Efficiency in Anaerobic/Aerobic/Anoxic Process

K. Soejima, K. Oki, A. Terada, S. Tsuneda, A. Hirata

Bioproc. Biosyst. Eng. 29(5) p.305 - 3132006/12-

電気泳動による微生物固定化空間内の基質輸送の促進と脱窒反応の向上

常田聡,賀來周一,林浩志,大串聡,寺田昭彦,平田彰

化学工学論文集 32(6) p.507 - 5132006/12-

生物学的リン除去プロセスの最新動向

近藤貴志,常田聡

化学工学 70(11) p.616 - 6202006/11-

分離培養技術の進展

青井議輝,波多徹,常田聡

生物工学 84(11) p.444 - 4472006/11-

Identification of Acetate- or Methanol-Assimilating Bacteria under Nitrate-Reducing Conditions by Stable-Isotope Probing

T. Osaka, S. Yoshie, S. Tsuneda, A. Hirata, N. Iwami, Y Inamori

Microbial Ecology 52(2) p.253 - 2662006/09-

Real-Time Quantitative LAMP (Loop-Mediated Isothermal Amplification of DNA) as a Simple Method for Monitoring Ammonia-Oxidizing Bacteria

Y. Aoi, M. Hosogai, S. Tsuneda

J. Biotechnol. 125(4) p.484 - 4912006/09-

Highly Sensitive Real-Time PCR Assay for Quantification of Toxic Cyanobacteria Based on Microcystin Synthetase A Gene

K. Furukawa, N. Noda, S. Tsuneda, T. Saito, T. Itayama, Y. Inamori

J. Biosci. Bioeng. 102(2) p.90 - 962006/08-

Molecular Analysis of Halophilic Bacterial Community for High-Rate Denitrification of Saline Industrial Wastewater

S. Yoshie, H. Makino, H. Hirosawa, K. Shirotani, S. Tsuneda, A. Hirata

Appl. Microbiol. Biotechnol. 72(1) p.182 - 1892006/08-

Rapid Autohydrogenotrophic Denitrification by a Membrane Biofilm Reactor Equipped with a Fibrous Support around a Gas-Permeable Membrane

A. Terada, S. Kaku, S. Matsumoto, S. Tsuneda

Biochem. Eng. J. 31(1) p.84 - 912006/08-

好気性グラニュールを用いた水処理技術

岸田直裕,常田聡

水処理技術 47(8) p.349 - 3552006/08-

Anaerobic/Oxic/Anoxic Granular Sludge Process as an Effective Nutrient Removal Process Utilizing Denitrifying Polyphosphate-Accumulating Organisms

N. Kishida, J.H. Kim, S. Tsuneda, R. Sudo

Water Res. 40(12) p.2303 - 23102006/07-

Sequencing Batch Membrane Biofilm Reactor for Simultaneous Nitrogen and Phosphorus Removal: Novel Application of Membrane-Aerated Biofilm

A. Terada, T. Yamamoto, S. Tsuneda, A. Hirata

Biotechnol. Bioeng. 94(4) p.730 - 7392006/06-

Evaluation of Sludge Reduction and Phosphorus Recovery Efficiencies in a New Advanced Wastewater Treatment System Using Denitrifying Polyphosphate Accumulating Organisms

Y. Suzuki, T. Kondo, K. Nakagawa, S. Tsuneda, A. Hirata, Y. Shimizu, Y. Inamori

Water Sci. Technol. 53(6) p.107 - 1132006/05-

マイクロバブル化オゾン酸化法および吸着脱リン法を組み込んだ新しい資源循環型排水処理システム

鈴木康之,近藤貴志,常田聡,稲森悠平

用水と廃水 48(5) p.424 - 4312006/05-

Affinity Capillary Electrophoresis with a DNA-nanoparticle Conjugate as a New Tool for Genotyping

K. Adachi, N. Noda, M. Nakashige, S. Tsuneda, T. Kanagawa

J. Chromatogr. A 1109(2) p.127 - 1312006/03-

Characteristics of Bacteria Showing High Denitrification Activity in Saline Wastewater

S. Yoshie, T. Ogawa, H. Makino, H. Hirosawa, S. Tsuneda, A. Hirata

Lett. Appl. Microbiol. 42(3) p.277 - 2832006/03-

Growth Characteristic of Anaerobic Ammonium-Oxidizing Bacteria in an Anaerobic Biological Filtrated Reactor

K. Isaka, Y. Date, S. Yoshie, T. Sumino, S. Tsuneda

Appl. Microbiol. Biotechnol. 70(1) p.47 - 522006/03-

High-Rate Nitrification Using Aerobic Granular Sludge

S. Tsuneda, M. Ogiwara, Y. Ejiri, A. Hirata

Water Sci. Technol. 53(3) p.147 - 1542006/03-

Long-Term Monitoring of the Succession of a Microbial Community in Activated Sludge from a Circulation Flush Toilet as a Closed System

T. Hoshino, T. Terahara, K. Yamada, H. Okuda, I. Suzuki, S. Tsuneda, A. Hirata, Y. Inamori

FEMS Microbiol. Ecol. 55(3) p.459 - 4702006/03-

Recovery of Sb(V) Using a Functional-Ligand-Containing Porous Hollow-Fiber Membrane Prepared by Radiation-Induced Graft Polymerization

H. Kawakita, K. Uezu, S. Tsuneda, K. Saito, M. Tamada, T. Sugo

Hydrometallurgy 81(3,4) p.190 - 1962006/03-

Feasibility of a Membrane-Aerated Biofilm Reactor to Achieve Controllable Nitrification

A. Terada, T. Yamamoto, R. Igarashi, S. Tsuneda, A. Hirata

Biochem. Eng. J. 28(2) p.123 - 1302006/02-

Simultaneous Nitrogen and Phosphorus Removal Using Denitrifying Phosphate-Accumulating Organisms in a Sequencing Batch Reactor

S. Tsuneda, T. Ohno, K. Soejima, A. Hirata

Biochem. Eng. J. 27(3) p.191 - 1962006/01-

ナノパーティクルを用いたアフィニティーキャピラリー電気泳動法による特定遺伝子の新規な検出手法の開発

足立賢,野田尚宏,中繁誠人,常田聡,金川貴博

化学工学シンポジウムシリーズ 79p.106 - 1102006/01-

生物学的窒素・リン除去プロセスにおける微生物生態と機能

近藤貴志,大坂利文,青井議輝,常田聡

用水と廃水 48(1) p.53 - 602006/01-

Molecular Analysis of Microbial Population Transition Associated with the Start of Denitrification in a Wastewater Treatment Process

T. Hoshino, T. Terahara, S. Tsuneda, A. Hirata, Y. Inamori

J. Appl. Microbiol. 99(5) p.1165 - 11752005/11-

繊維化スラグの複合的利用による低濃度重金属汚染水処理

常田聡,酒井寿郎,林浩志

水環境学会誌 28(11) p.651 - 6552005/11-

Comparison of Spatial Organization in Top-down- and Membrane-aerated Biofilms: A Numerical Study

A. Bell, Y. Aoi, A. Terada, S. Tsuneda, A. Hirata

Water Sci. Technol. 52(7) p.173 - 1802005/10-

窒素除去プロセスにおける複合微生物系解析とその利用

青井議輝,大坂利文,常田聡

水環境学会誌 28(8) p.474 - 4782005/08-

Single-Stage Autotrophic Nitrogen-Removal Process Using a Composite Matrix Immobilizing Nitrifying and Sulfur-Denitrifying Bacteria

Y. Aoi, Y. Shiramasa, E. Kakimoto, S. Tsuneda, A. Hirata, T. Nagamune

Appl. Microbiol. Biotechnol. 68(1) p.124 - 1302005/07-

Elucidation of Dominant Effect on Initial Bacterial Adhesion onto Polymer Surfaces Prepared by Radiation-Induced Graft Polymerization

A. Terada, A. Yuasa, S. Tsuneda, A. Hirata, A. Katakai, M. Tamada

Colloids and Surf. B: Biointerfaces 43(2) p.99 - 1072005/06-

ビール粕成形炭による観賞魚用水槽の水質浄化

岡本裕行,芝田隼次,村山憲弘,山本秀樹,塚元祐二,飯塚梨加,常田聡

環境資源工学 52(1) p.25 - 312005/05-

Characteristics and Applicability of Nitrifying Granules Produced in an Aerobic Upflow Fluidized Bed Reactor

S. Tsuneda, Y. Ejiri, M. Ogiwara, T. Nagano, A. Hirata

Aerobic Granular Sludge, Water Environmental Management Series (WEMS) p.15 - 242005/04-

Characterization of Denitrifying Polyphosphate-Accumulating Organisms in Activated Sludge Based on Nitrite Reductase Gene

S. Tsuneda, R. Miyauchi, T. Ohno, A. Hirata

J. Biosci. Bioeng. 99(4) p.403 - 4072005/04-

Quantification of Genetically Modified Soybean by Quenching Probe Polymerase Chain Reaction

H. Tani, N. Noda, K. Yamada, S. Kurata, S. Tsuneda, A. Hirata, T. Kanagawa

J. Agric. Food Chem. 53(7) p.2535 - 25402005/04-

Effect of Salinity on Nitrous Oxide Emission in the Biological Nitrogen Removal Process for Industrial Wastewater

S. Tsuneda, M. Mikami, Y. Kimochi, A. Hirata

J. Hazard. Mater. 119(1~3) p.93 - 982005/03-

ステップ流入式嫌気/好気/無酸素プロセスによる窒素・リン同時除去

常田聡,大野高史,副島孝一,平田彰

用水と廃水 47(3) p.230 - 2362005/03-

脱窒性リン蓄積細菌を利用した下水処理技術およびリン資源回収の可能性

常田聡,大野高史,副島孝一,平田彰

環境バイオテクノロジー学会誌 4(2) p.95 - 992005/03-

リアルタイム制御法を用いた新規窒素除去プロセスの開発

岸田直裕,金主鉉,須藤隆一,佐々木弘,常田聡

ケミカルエンジニヤリング 50(3) p.108 - 1132005/02-

Automatic Control Strategy for Biological Nitrogen Removal of Low C/N Wastewater in a Sequencing Batch Reactor

N. Kishida, J.H. Kim, M. Chen, S. Tsuneda, H. Sasaki, R. Sudo

Water Sci. Technol. 50(10) p.45 - 502004/12-

Monitoring the Microbial Population Dynamics at the Start-up Stage of Wastewater Treatment Reactor by Terminal Restriction Fragment Length Polymorphism Analysis Based on 16S rDNA and rRNA Gene Sequences

T. Terahara, T. Hoshino, S. Tsuneda, A. Hirata, Y. Inamori

J. Biosci. Bioeng. 98(6) p.425 - 4282004/12-

Quantitative Analysis of amoA mRNA Expression as a New Biomarker of Ammonia Oxidation Activities in a Complex Microbial Community

Y. Aoi, Y. Masaki, S. Tsuneda, A. Hirata

Lett. Appl. Microbiol. 39(6) p.477 - 4822004/12-

硝化グラニュールを利用した高塩濃度産業廃水処理

星野辰彦,広沢英信,松川忠史,小川知幸,江尻慶央,常田聡,平田彰

日本水処理生物学会誌 40(4) p.137 - 1422004/12-

Significance of Cell Electrokinetic Properties Determined by Soft-Particle Analysis in Bacterial Adhesion onto a Solid Surface

S. Tsuneda, H. Aikawa, H. Hayashi, A. Hirata

J. Colloid Interface Sci. 279(2) p.410 - 4172004/11-

Removal of Antimony (III) Using Polyol-Ligand-Containing Porous Hollow-Fiber Membranes

T. Saito, S. Tsuneda, A. Hirata, S. Nishiyama, K. Saito, K. Saito, K. Sugita, K. Uezu, M. Tamada, T. Sugo

Sep. Sci. Technol. 39(13) p.3011 - 30222004/09-

高度リン回収および余剰汚泥減容化を志向した排水処理プロセスの開発および評価

稲森悠平,中川和哉,鈴木康之,近藤貴志,常田聡,平田彰

用水と廃水 46(8) p.671 - 6772004/08-

Expression of amoA mRNA in Wastewater Treatment Processes Examined by Competitive RT-PCR

Y. Aoi, Y. Shiramasa, Y. Masaki, S. Tsuneda, A. Hirata, A. Kitayama, T. Nagamune

J. Biotechnol. 111(2) p.111 - 1202004/07-

Comparative Analysis of Genetic Diversity and Expression of amoA in Wastewater Treatment Process

Y. Ebie, N. Noda, H. Miura, M. Matsumura, S. Tsuneda, A. Hirata, Y. Inamori

Appl. Microbiol. Biotechnol. 64(5) p.740 - 7442004/06-

Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system

S. Yoshie, N. Noda, S. Tsuneda, A. Hirata, Y. Inamori

FEMS Microbiol. Lett. 235(1) p.183 - 1892004/06-

Enhancement of Biofilm Formation onto Surface-Modified Hollow-Fiber Membranes and Its Application to a Membrane-Aerated Biofilm Reactor

A. Terada, T. Yamamoto, K. Hibiya, S. Tsuneda, A. Hirata

Water Sci. Technol. 49(11,12) p.263 - 2682004/06-

Formation Mechanism of Nitrifying Granules Observed in an Aerobic Upflow Fluidized Bed (AUFB) Reactor

S. Tsuneda, Y. Ejiri, T. Nagano, A. Hirata

Water Sci. Technol. 49(11,12) p.27 - 342004/06-

Simple Prediction of Oxygen Penetration Depth in Biofilms for Wastewater Treatment

K. Hibiya, J. Nagai, S. Tsuneda, A. Hirata

Biochem. Eng. J. 19(1) p.61 - 682004/06-

Simplified Modeling of Simultaneous Reaction Kinetics of Carbon Oxidation and Nitrification in Biofilm Processes

S. Tsuneda, J. Auresenia, K. Hibiya, A. Hirata

Eng. Life Sci. 4(3) p.239 - 2462004/06-

Structure of polyol-igand-containing polymer brush on the porous membrane for antimony(III) binding

T. Saito, H. Kawakita, K. Uezu, S. Tsuneda, A. Hirata, K. Saito, M. Tamada, T. Sugo

J. Membr. Sci. 236(1,2) p.65 - 712004/06-

Transition of Bacterial Spatial Organization in a Biofilm Monitored by FISH and Subsequent Image Analysis

Y. Aoi, S. Tsuneda, A. Hirata

Water Sci. Technol. 49(11,12) p.365 - 3702004/06-

脱窒性リン蓄積細菌を用いた水処理技術

常田聡,大野高史,安祚煥,平田彰

水処理技術 45(6) p.261 - 2662004/06-

Salinity Decreases Nitrite Reductase Gene Diversity in Denitrifying Bacteria of Wastewater Treatment Systems

S. Yoshie, N. Noda, S. Tsuneda, A. Hirata, Y. Inamori

Appl. Environ. Microbiol. 70(5) p.3152 - 31572004/05-

T-RFLP法による排水処理細菌叢の迅速モニタリング

星野辰彦,寺原猛,常田聡,平田彰,稲森悠平

用水と廃水 46(5) p.408 - 4122004/05-

メンブレンエアレーション法を応用した単一槽内有機物・窒素同時除去システム

寺田昭彦,日比谷和明,常田聡,平田彰

用水と廃水 46(2) p.148 - 1552004/02-

界面特性を利用した細菌細胞の付着・脱着制御法

林浩志,二瓶智也,生木大志,常田聡,平田彰,佐々木弘

資源と素材 120(1) p.54 - 592004/01-

Characterization of Nitrifying Granules Produced in an Aerobic Upflow Fluidized Bed Reactor

S. Tsuneda, T. Nagano, T. Hoshino, Y. Ejiri, N. Noda, A. Hirata

Water Res. 37(20) p.4965 - 49732003/12-

Degradation of Xenobiotic Substances Using Sulfate-Reducing Bacteria in UASB Reactor

S. Tsuneda, T. Shiono, K. Nakamura, T. Dogan, A. Hirata

Water Sci. Technol. 48(11,12) p.227 - 2342003/12-

Effects of SRT and DO on N2O Reductase Activity in an Anoxic-Oxic Activated Sludge System

N. Noda, N. Kaneko, M. Mikami, Y. Kimochi, S. Tsuneda, A. Hirata, M. Mizuochi, Y. Inamori

Water Sci. Technol. 48(11,12) p.363 - 3702003/12-

RT-PCR法を用いた生活排水処理プロセスにおけるアンモニア酸化細菌の活性発現のモニタリング解析

蛯江美孝,三浦英智,野田尚宏,松村正利,常田聡,平田彰,水落元之,稲森悠平

日本水処理生物学会誌 39(4) p.199 - 2072003/12-

ビール粕を原料とする成形炭の水質浄化特性

岡本裕行,八木紀依,井上雅夫,山崎秀一,石田清治,芝田隼次,山本秀樹,林浩志,塩野貴史,常田聡,平田彰

環境資源工学 50(4) p.165 - 1742003/12-

放射性同位体トレーサー法を用いた水圏生態系マイクロコズムに及ぼすリンの影響評価

近藤貴志,板山朋聡,常田聡,平田彰,稲森悠平

日本水処理生物学会誌 39(4) p.175 - 1812003/12-

硫酸塩還元細菌を利用したFe-EDTA分解除去プロセスの構築

常田聡,塩野貴史,中村和之,平田彰

日本水処理生物学会誌 39(4) p.167 - 1742003/12-

In Situ PCR for Visualizing of Distribution of a Functional Gene "amoA" in a Biofilm Regardless of Activity

T. Hoshino, S. Tsuneda, A. Hirata, Y. Inamori

J. Biotechnol. 105(1,2) p.33 - 402003/10-

Adsorption Effect on the Dynamic Response of Biochemical Reaction in a Biofilm Reactor for Wastewater Treatment

S. Tsuneda, Y. Inoue, J. Auresenia, A. Hirata

Eng. Life Sci. 3(9) p.371 - 3752003/09-

Influence of Growth Phase on Bacterial Cell Electrokinetic Characteristics Examined by Soft Particle Electrophoresis Theory

H. Hayashi, H. Seiki, S. Tsuneda, A. Hirata, H. Sasaki

J. Colloid Interface Sci. 264(2) p.565 - 5682003/09-

Photooxidation of PCE in Gas Phase in UV-Bubble Column Reactor with H2O2

D. Alibegic, S. Tsuneda, A. Hirata

Water Intelligence Online 2003/09-

スラグウールを付着担体とする固定床型硫酸還元バイオリアクターによる希薄廃水からのカドミウム回収

林浩志,勝賀瀬暢一,常田聡,平田彰,佐々木弘

資源と素材 119(9) p.559 - 5632003/09-

Extracellular Polymeric Substances Responsible for Bacterial Adhesion onto Solid Surface

S. Tsuneda, H. Aikawa, H. Hayashi, A. Yuasa, A. Hirata

FEMS Microbiol. Lett. 223(2) p.287 - 2922003/06-

Oxidation of Tetrachloroethylene in a Bubble Column Photochemical Reactor Applying the UV/H2O2 Technique

D. Alibegic, S. Tsuneda, A. Hirata

Can. J. Chem. Eng. 81(3,4) p.733 - 7402003/06-

Quinone Profiles Reflecting Population Dynamics of Denitrifying Phosphate-Accumulating Organisms

S. Tsuneda, T. Ohno, J. Ahn, T. Daidou, A. Hirata

Microbes and Environments 18(2) p.69 - 732003/06-

Influence of Extracellular Polymers on Electrokinetic Properties of Heterotrophic Bacterial Cells Examined by Soft Particle Electrophoresis Theory

S. Tsuneda, J. Jung, H. Hayashi, H. Aikawa, A. Hirata, H. Sasaki

Colloids and Surf. B: Biointerfaces 29(2,3) p.181 - 1882003/05-

High-Speed Recovery of Antimony Using Chelating Porous Hollow-Fiber Membrane

S. Nishiyama, K. Saito, K. Saito, K. Sugita, K. Sato, M. Akiba, T. Saito, S. Tsuneda, A. Hirata, M. Tamada, T. Sugo

J. Membr. Sci. 214(2) p.275 - 2812003/04-

中空糸メンブレンを用いた新しい水処理技術

寺田昭彦,日比谷和明,常田聡,平田彰

水処理技術 44(4) p.153 - 1642003/04-

Comparison of Detection Specificity of Nitrifying Bacteria in Biofilm using Fluorescence in situ Hybridization and in situ Fluorescence Antibody Methods

N. Noda, Y. Ebie, M. Matsumura, S. Tsuneda, A. Hirata, Y. Inamori

Water Sci. Technol. 47(5) p.129 - 1322003/03-

Nitrogen Removal Characteristics and Biofilm Analysis of a Membrane-Aerated Biofilm Reactor Applicable to High-Strength Nitrogenous Wastewater Treatment

A. Terada, K. Hibiya, J. Nagai, S. Tsuneda, A. Hirata

J. Biosci. Bioeng. 95(2) p.170 - 1782003/02-

UV-Bubble Column Reactor (UV-BCR) for Photolytic Removal of Tetrachloroethylene (PCE) from the Vapor Phase: Methodological Approach

D. Alibegic, S. Tsuneda, A. Hirata

J. Chem. Eng. Jpn. 36(2) p.178 - 1862003/02-

独立栄養細菌を用いた硝化・脱窒同時進行型反応場の開発

青井議輝,白政優子,常田聡,平田彰

用水と廃水 45(2) p.129 - 1332003/02-

Simultaneous Nitrification and Denitrification by Controlling Vertical and Horizontal Microenvironment in a Membrane-Aerated Biofilm Reactor

K. Hibiya, A. Terada, S. Tsuneda, A. Hirata

J. Biotechnol. 100(1) p.23 - 322003/01-

Characterization of Microbial Community in Nitrogen Removal Process of Metallurgic Wastewater by PCR-DGGE

S. Yoshie, N. Noda, T. Miyano, S. Tsuneda, A. Hirata, Y. Inamori

Water Sci. Technol. 46(11,12) p.93 - 982002/12-

Community Analysis of Nitrifying Bacteria in an Advanced and Compact Gappei-Johkasou by FISH and PCR-DGGE

Y. Ebie, M. Matsumura, N. Noda, S. Tsuneda, A. Hirata, Y. Inamori

Water Sci. Technol. 46(11,12) p.105 - 1112002/12-

Inhibition Effect of Chlorine Ion on Hydroxyl Radical Generation in UV-H2O2 Process

S. Tsuneda, Y. Ishihara, M. Hamachi, A. Hirata

Water Sci. Technol. 46(11,12) p.33 - 382002/12-

脱窒性リン蓄積細菌を利用した新しい高度排水処理プロセス

常田聡,安祚煥,大道智孝,大野高史,平田彰

水環境学会誌 25(12) p.751 - 7552002/12-

Dynamic Modeling and Simulation of a Three-Phase Fluidized Bed Batch Process for Wastewater Treatment

S. Tsuneda, J. Auresenia, T. Morise, A. Hirata

Process Biochem. 38(4) p.599 - 6042002/11-

放射線グラフト重合法で作製した多孔性膜を用いた金属イオンの高効率捕集

斎藤加織,斎藤恭一,杉田和之,常田聡,平田彰,須郷高信

科学と工業 76(11) p.565 - 5712002/11-

有用微生物固定化技術と排水高度処理への応用

常田聡

最近の化学工学 54p.128 - 1412002/11-

Detection and Quantification of Expression of amoA by Competitive Reverse Transcription-PCR

Y. Ebie, H. Miura, N. Noda, M. Matsumura, S. Tsuneda, A. Hirata, Y. Inamori

Water Sci. Technol. 46(1, 2) p.281 - 2882002/09-

PCR-DGGE Analysis of Denitrifying Bacteria in a Metallurgic Wastewater Treatment Process

N. Noda, Y. Yoshie, T. Miyano, S. Tsuneda, A. Hirata, Y. Inamori

Water Sci. Technol. 46(1, 2) p.333 - 3362002/09-

Real-Time Monitoring of Ammonia-Oxidizing Activity in a Nitrifying Biofilm by amoA mRNA Analysis

Y. Aoi, Y. Shiramasa, S. Tsuneda, A. Hirata, A. Kitayama, T. Nagamune

Water Sci. Technol. 46(1, 2) p.439 - 4422002/09-

Transformation of Phosphorus and Relevant Intracellular Compounds by a Phosphorus-Accumulating Enrichment Culture in the Presence of Both the Electron Acceptor and Electron Donor

J. Ahn, T. Daidou, S. Tsuneda, A. Hirata

Biotechnol. Bioeng. 79(1) p.83 - 932002/07-

吸着脱リン法を導入した資源回収型リン除去技術

高井智丈,宮坂章,稲森悠平,小松央子,小沼和博,中川和哉,常田聡

用水と廃水 44(7) p.604 - 6102002/07-

Kinetic Model for Dynamic Response of Three-Phase Fluidized Bed Biofilm Reactor for Wastewater Treatment

S. Tsuneda, J. Auresenia, Y. Inoue, Y. Hashimoto, A. Hirata

Biochem. Eng. J. 10(1) p.31 - 372002/02-

Characterization of Denitrifying Phosphate-Accumulating Organisms Cultivated under Different Electron Acceptor Conditions using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Assay

J. Ahn, T. Daidou, S. Tsuneda, A. Hirata

Water Res. 36(2) p.403 - 4122002/01-

Three-Dimensional Immobilization of Bacterial Cells with a Fibrous Network and its Application in a High-Rate Fixed-bed Nitrifying Bioreactor

H. Hayashi, M. Ono, S. Tsuneda, A. Hirata

J. Chem. Eng. Jpn. 35(1) p.68 - 752002/01-

Metabolic Behaviors of Denitrifying Phosphate-Accumulating Organisms under Nitrate and Nitrite Electron Acceptor Conditions

J. Ahn, T. Daidou, S. Tsuneda, A. Hirata

J. Biosci. Bioeng. 92(5) p.442 - 4462001/12-

Selection and Dominance Mechanisms of Denitrifying Phosphate-Accumulating Organisms in Biological Phosphorus Removal Process

J. Ahn, T. Daidou, S. Tsuneda, A. Hirata

Biotechnol. Lett. 23(24) p.2005 - 20082001/12-

貴金属回収工程から排出される硝酸含有廃水の生物学的窒素除去

常田聡,宮野知子,小川知幸,吉江幸子,平田 彰,大田有香,磯部公信,稲垣 肇,片山 卓

日本水処理生物学会誌 37(4) p.141 - 1492001/12-

地域環境保全のための嫌気好気生物膜法による畜産排水の高度処理 〜脱窒性リン蓄積細菌の優占化技術と栄養塩除去プロセスへの応用〜

常田聡,安祚煥,大道智孝,平田彰

環境科学総合研究所年報 20p.7 - 112001/12-

Direct Detection by in situ PCR of the amoA Gene in Biofilm Resulting from a Nitrogen Removal Process

T. Hoshino, N. Noda, S. Tsuneda, A. Hirata, Y. Inamori

Appl. Environ. Microbiol. 67(11) p.5261 - 52662001/11-

Kinetics of Tetrachloroethylene (PCE) Gas Degradation and Byproducts Formation during UV/H2O2 Treatment in UV-Bubble Column Reactor

D. Alibegic, S. Tsuneda, A. Hirata

Chem. Eng. Sci. 56(21, 22) p.6195 - 62032001/11-

Microbial Community Analysis in the Denitrification Process of Saline Wastewater by Denaturing Gradient Gel Electrophoresis of PCR-Amplified 16S rRNA and the Cultivation Method

S. Yoshie, N. Noda, T. Miyano, S. Tsuneda, A. Hirata, Y. Inamori

J. Biosci. Bioeng. 92(4) p.346 - 3532001/11-

Rapid Recovery of Bacterial Cells from a Stable Dispersion by Heterocoagulation to a Fibrous Collector

H. Hayashi, T. Nihei, M. Ono, S. Tsuneda, A. Hirata, H. Sasaki

J. Colloid Interface Sci. 243(1) p.109 - 1152001/10-

TOC Removal of Diethylene Glycol Mono-n-hexyl Ether Synthetic Wastewater and 1,4-Butanediol Diglycidyl Ether Synthetic Wastewater with H2O2/UV in a Batch Reactor

W. J. Hou, S. Tsuneda, A. Hirata

J. Chem. Eng. Jpn. 34(10) p.1279 - 12842001/10-

Efficient TOC Removal of Wastewater Containing 1,2-Naphthoquinone-2-diazido-5-sulfonic Acid Sodium Salt with H2O2/UV in a Batch Reactor

W. J. Hou, S. Tsuneda, A. Hirata

J. Chem. Eng. Jpn. 34(9) p.1084 - 10902001/09-

Biological Nitrogen Removal from Industrial Wastewater Discharged from Metal Recovery Processes

A. Hirata, Y. Nakamura, S. Tsuneda

Water Sci. Technol. 44(2, 3) p.171 - 1792001/08-

Soft Particle Analysis of Bacterial Cells and its Interpretation of Cell Adhesion Behaviors in terms of DLVO Theory

H. Hayashi, S. Tsuneda, A. Hirata, H. Sasaki

Colloids and Surf. B: Biointerfaces 22(2) p.149 - 1572001/08-

TOC Removal of 2,4,5-Trichlorophenol Synthetic Wastewater with H2O2/UV in a Batch Reactor

W. J. Hou, S. Tsuneda, A. Hirata

J. Chem. Eng. Jpn. 34(8) p.1049 - 10512001/08-

Visualization of Microscale Distribution of Nitrifying Bacteria in Biofilms Formed in Various Type Wastewater Treatment Processes

Y. Aoi, T. Miyoshi, T. Okamoto, S. Tsuneda, A. Kitayama, E. Kayano, T. Nagamune, A. Hirata

Advances in Water and Wastewater Treatment Technology -Molecular Technology, Nutrient Removal, Sludge Reduction, and Environmental Health- p.141 - 1512001/08-

Direct Observation of Nitrifying Biofilms on Particles by Electron Microscopy

A. Hirata, A. A. Meutia, S. Tsuneda

Jpn. J. Water Treatment Biol. 37(2) p.87 - 922001/06-

包括型および付着型PEG担体で固定した硝化細菌の抗原抗体法による挙動解析

野田尚宏,蛯江美孝,生田創,常田聡,平田彰,松村正利,角野立夫,稲森悠平

日本水処理生物学会誌 37(2) p.77 - 862001/06-

Enhancement of Nitrifying Biofilm Formation Using Selected EPS Produced by Heterotrophic Bacteria

S. Tsuneda, S. Park, H. Hayashi, J. Jung, A. Hirata

Water Sci. Technol. 43(6) p.197 - 2042001/05-

The Role of Electrokinetic Properties on Adhesion of Nitrifying Bacteria to Solid Surfaces

H. Hayashi, S. Tsuneda, A. Hirata, H. Sasaki

Studies in Surface Science and Catalysis 132p.293 - 2962001/05-

粉砕生ゴミを含む厨芥排水の生物処理

常田聡,丁在国,中村和之,日比谷和明,平田彰

用水と廃水 43(5) p.381 - 3852001/05-

TOC Removal of Raw Industrial Wastewater from LSI Photo-Resist Processing with H2O2/UV in a Batch Reactor

W. J. Hou, S. Tsuneda, A. Hirata

J. Chem. Eng. Jpn. 34(3) p.444 - 4472001/03-

アンチモン含有廃水の処理技術

齋藤智宣,常田聡,斎藤恭一,平田彰

水処理技術 42(3) p.103 - 1112001/03-

火力発電所復水脱塩装置再生廃水からの生物学的窒素除去

常田聡,三好朋子,平田彰

日本水処理生物学会誌 37(1) p.9 - 172001/03-

Simultaneous Nitrogen and Phosphorus Removal from Nutrient-Rich Wastewater -I. Characterization of Denitrifying Phosphate-Accumulating Organisms-

S. Tsuneda, J. Ahn, T. Daidou, A. Hirata

Ann. Report Interdiscipl. Res. Inst. Environ. Sci. 19p.67 - 752000/12-

Microbial Ecology of Nitrifying Bacteria in Wastewater Treatment Process Examined by Fluorescence in situ Hybridization

Y. Aoi, T. Miyoshi, T. Okamoto, S. Tsuneda, A. Hirata, A. Kitayama, T. Nagamune

J. Biosci. Bioeng. 90(3) p.234 - 2402000/10-

Tailoring of Highly Efficient Nitrifying Biofilms in Fluidized Bed for Ammonia-Rich Industrial Wastewater Treatment

S. Tsuneda, T. Miyoshi, Y. Aoi, A. Hirata

Water Sci. Technol. 42(3, 4) p.357 - 3622000/10-

The Rapid Quantification and Detection of Nitrifying Bacteria by Using Monoclonal Antibody Method

H. Ikuta, N. Noda, Y. Ebie, A. Hirata, S. Tsuneda, M. Matsumura, Y. Inamori

Water Sci. Technol. 42(3, 4) p.1 - 72000/10-

感光剤製造工程廃液の生物処理

平田彰,島本敦史,飯田宏幸,常田聡

用水と廃水 42(9) p.775 - 7802000/09-

Rapid Quantification and in situ Detection of Nitrifying Bacteria in Biofilms by Monoclonal Antibody Method

N. Noda, H. Ikuta, Y. Ebie, A. Hirata, S. Tsuneda, M. Matsumura, T. Sumino, Y. Inamori

Water Sci. Technol. 41(4, 5) p.301 - 3082000/07-

Evaluation of Kinetic Parameters of Biochemical Reaction in Three-Phase Fluidized Bed Biofilm Reactor for Wastewater Treatment

A. Hirata, T. Takemoto, K. Ogawa, J. Auresenia, S. Tsuneda

Biochem. Eng. J. 5(2) p.165 - 1712000/04-

Formation and Characteristics of Nitrifying Biofilm on a Membrane Modified with Positively-Charged Polymer Chains

K. Hibiya, S. Tsuneda, A. Hirata

Colloids and Surf. B: Biointerfaces 18(2) p.105 - 1122000/03-

Effects of Oxygen Supply Condition and Specific Biofilm Interfacial Area on Phenol Removal Rate in a Three-Phase Fluidized Bed Bioreactor

A. Hirata, A. A. Meutia, M. Osawa, M. Arai, S. Tsuneda

Can. J. Chem. Eng. 78(2) p.95 - 1012000/02-

写真廃液中の難分解性有機物の微生物分解

平田彰,山下秀人,常田聡

用水と廃水 41(9) p.808 - 8121999/09-

Radiation-Induced Graft Polymerization is the Key to Develop High-Performance Functional Materials for Protein Purification

K. Saito, S. Tsuneda, M. Kim, N. Kubota, K. Sugita, T. Sugo

Radiat. Phys. Chem. 54(5) p.517 - 5251999/05-

紫外線照射と過酸化水素逐次添加を併用したジクロロメタンの高速分解処理

平田彰,石井純,常田聡

水環境学会誌 22(5) p.396 - 4021999/05-

Tailoring of a Smooth Polycrystalline Gold Surface as a Suitable Anchoring Site for a Self-Assembled Monolayer

S. Tsuneda, T. Ishida, N. Nishida, M. Hara, H. Sasada, W. Knoll

Thin Solid Films 339(1, 2) p.142 - 1471999/02-

写真廃液の再生とゼロエミッション化

平田彰,武井浩樹,常田聡

化学工業 50(2) p.113 - 1181999/02-

Kinetics of Biological Treatment of Phenolic Wastewater in Three-Phase Fluidized Bed Containing Biofilm and Suspended Sludge

A. Hirata, M. Noguchi, N. Takeuchi, S. Tsuneda

Water Sci. Technol. 38(8, 9) p.205 - 2121998/12-

High Resolution X-Ray Photoelectron Spectroscopy Measurements of Octadecanethiol Self-Assembled Monolayers on Au(111)

T. Ishida, M. Hara, I. Kojima, S. Tsuneda, N. Nishida, H. Sasabe, W. Knoll

Langmuir 14(8) p.2092 - 20961998/04-

嫌気好気生物膜法による産業廃水の高度処理

平田彰,常田聡

ケミカルエンジニヤリング 43(3) p.188 - 1921998/03-

Fluorescence Study on the Conformational Change of an Amino Group-Containing Polymer Chain Grafted onto a Polyethylene Microfiltration Membrane

S. Tsuneda, T. Endo, K. Saito, K. Sugita, K. Horie, T. Yamashita, T. Sugo

Macromolecules 31(2) p.366 - 3701998/01-

Treatment of Photographic Processing Wastewater Using Anaerobic-Aerobic Biofilm Reactor

A. Hirata, H. Lee, S. Tsuneda, T. Takai

Water Sci. Technol. 36(12) p.91 - 991997/12-

Local Mobility of Polymer Chain Grafted onto Polyethylene Monitored by Fluorescence Depolarization

S. Tsuneda, T. Endo, K. Saito, K. Sugita, K. Horie, T. Yamashita, T. Sugo

Chem. Phys. Lett. 275(3, 4) p.203 - 2101997/08-

Surface-Conditioning Effect of Gold Substrates on Octadecanethiol Self-Assembled Monolayer Growth

T. Ishida, S. Tsuneda, N. Nishida, M. Hara, H. Sasabe, W. Knoll

Langmuir 13(17) p.4638 - 46431997/08-

Alkyl Chain Length Effect on Growth Kinetics of n-Alkanethiol Self-Assembled Monolayers on Gold Studied by X-ray Photoelectron Spectroscopy

T. Ishida, N. Nishida, S. Tsuneda, M. Hara, H. Sasabe, W. Knoll

Jpn. J. Appl. Phys. Part2-Lett. 35(12B) p.L1710 - L17131996/12-

Ring-Opening Reaction of Poly-GMA Chain Grafted onto Porous Membrane

M. Kim, S. Kiyohara, S. Konishi, S. Tsuneda, K. Saito, T. Sugo

J. Membr. Sci. 117(1, 2) p.33 - 381996/08-

Hydrodynamic Evaluation of Three-Dimensional Adsorption of Protein to Polymer Chain Grafted onto Porous Substrate

S. Tsuneda, H. Kagawa, K. Saito, T. Sugo

J. Colloid Interface Sci. 176(1) p.95 - 1001995/12-

Novel Ion-Exchange Membranes for Electrodialysis Prepared by Radiation-Induced Graft Polymerization

S. Tsuneda, K. Saito, H. Mitsuhara, T. Sugo

J. Electrochem. Soc. 142(11) p.3659 - 36631995/11-

Highly Efficient Enzyme Recovery Using a Porous Membrane with Immobilized Tentacle Polymer Chains

S. Matoba, S. Tsuneda, K. Saito, T. Sugo

Bio/Technology 13(8) p.795 - 7971995/08-

Protein Adsorption Characteristics of Porous and Tentacle Anion-Exchange Membrane Prepared by Radiation-Induced Graft Polymerization

S. Tsuneda, K. Saito, T. Sugo, K. Makuuchi

Radiat. Phys. Chem. 46(2) p.239 - 2451995/08-

High-Throughput Processing of Proteins Using a Porous and Tentacle Anion-Exchange Membrane

S. Tsuneda, K. Saito, S. Furusaki, T. Sugo

J. Chromatogr. 689(2) p.211 - 2181995/01-

Hydrolysis of Methyl Acetate and Sucrose in SO3H-Group-Containing Grafted Polymer Chains Prepared by RIGP

T. Mizota, S. Tsuneda, K. Saito, T. Sugo

Ind. Eng. Chem. Res. 33(9) p.2215 - 22191994/09-

Sulfonic Acid Catalysts Prepared by Radiation-Induced Graft Polymerization

T. Mizota, S. Tsuneda, K. Saito, T. Sugo

J. Catal. 149(1) p.243 - 2451994/09-

Radiation-Induced Graft Polymerization of Vinyl Benzyltrimethylammonium Chloride onto Polyethylene Film

Y. C. Nho, T. Sugo, S. Tsuneda, K. Makuuchi

J. Appl. Polym. Sci. 51(7) p.1269 - 12751994/02-

Binding of Lysozyme onto a Cation-Exchange Microporous Membrane Containing Tentacle-Type Grafted Polymer Branches

S. Tsuneda, H. Shinano, K. Saito, S. Furusaki

Biotechnol. Prog. 10(1) p.76 - 811994/01-

Attachment of Sulfonic Acid Group to Various Shapes of PE, PP and PTFE by Radiation-Induced Graft Polymerization

S. Sugiyama, S. Tsuneda, K. Saito, S. Furusaki, T. Sugo, K. Makuuchi

Reactive Polymers 21(3) p.187 - 1911993/12-

Simple Introduction of Sulfonic Acid Group onto Polyethylene by Radiation-Induced Cografting of Sodium Styrenesulfonate with Hydrophilic Monomers

S. Tsuneda, K. Saito, S. Furusaki, T. Sugo, K. Makuuchi

Ind. Eng. Chem. Res. 32(7) p.1464 - 14701993/07-

Ion Exchange of Lysozyme during Permeation across a Microporous Sulfopropyl-Group-Containing Hollow Fiber

H. Shinano, S. Tsuneda, K. Saito, S. Furusaki, T. Sugo

Biotechnol. Prog. 9(2) p.193 - 1981993/03-

Preparation of Microfiltration Membranes Containing Anion-Exchange Group

K. Kobayashi, S. Tsuneda, K. Saito, H. Yamagishi, S. Furusaki, T. Sugo

J. Membr. Sci. 76(2, 3) p.209 - 2181993/02-

Water/Acetone Permeability of Porous Hollow-Fiber Membrane Containing Diethylamino Groups on the Grafted Polymer Branches

S. Tsuneda, K. Saito, S. Furusaki, T. Sugo, I. Ishigaki

J. Membr. Sci. 71(1, 2) p.1 - 121992/07-

Metal Collection Using Chelating Hollow Fiber Membrane

S. Tsuneda, K. Saito, S. Furusaki, T. Sugo, J. Okamoto

J. Membr. Sci. 58(2) p.221 - 2341991/05-

Books And Publication

生命科学概論 ー環境・エネルギーから医療までー

常田聡

朝倉書店2012/04-

Detali

ISBN:978-4-254-17151-8

基礎から理解する化学4 化学工学

常田聡

みみずく舎2012/04-

Detali

ISBN:978-4-86399-142-2

難培養微生物研究の最新技術II

古川和寛,阿部洋,伊藤嘉浩,常田聡

シーエムシー出版2010/04-

Detali

ISBN:978-4-7813-0183-9

リン資源の回収と有効利用

蛯江美孝,近藤貴志,常田聡,稲森悠平

サイエンス&テクノロジー2009/11-

Detali

ISBN:978-4-903413-76-1

マリンメタゲノムの有効利用

野田 尚宏,関口勇地,松田 泰嘉,谷 英典,常田 聡

シーエムシー出版2009/07-

Detali

ISBN:978-4-7813-0114-3

バイオフィルムの基礎と制御

松本慎也,常田聡

株式会社エヌ・ティー・エス2008/02-

Detali

ISBN:978-4-86043-150-1

排水・汚水処理技術集成

常田聡,近藤貴志

株式会社エヌ・ティー・エス2007/05-

一細胞定量解析の最前線 ライフサーベイヤ構築に向けて

阿部洋,古川和寛,常田聡,伊藤嘉浩

シーエムシー出版2006/12-

複合微生物系の産業利用と新産業創出

伊達康博,青井議輝,常田聡

シーエムシー出版2006/07-

Aerobic Granular Sludge, Water Environmental Management Series (WEMS)

S. Tsuneda, Y. Ejiri, M. Ogiwara, T. Nagano, A. Hirata

IWA Publishing2005/04-

最近の化学工学 54「バイオプロセスエンジニアリングの新展開-アップストリームからダウンストリームまで-」

常田聡

化学工業社2002/11-

Advanced in Water and Wastewater Treatment Technology -Molecular Technology, Nutrient Removal, Sludge Reduction, and Environmental Health-

Y. Aoi, T. Miyoshi, T. Okamoto, S. Tsuneda, A. Kitayama, E. Kayano, T. Nagamune, A. Hirata

Elsevier2000/09-

Lecture And Oral

Exploring the intestinal bacteria involved in the regulation of immune-metabolism

Hindgut Club JAPAN symposium2014/12/06

Detail

Poster presentation

The Effectiveness of Low Temperature Cultivation (LTC) for Tissue Engineering

7th World Congress on Preventive and Regenerative Medicine2014/11/06

Detail

Oral presentation(general)

Fabrication of Biomimetic Three-Dimensional Muscle Tissue Construct with Well-Organized Orientation

7th World Congress on Preventive and Regenerative Medicine2014/11/06

Detail

Oral presentation(general)

MPL mutation analysis of Ph-negative myeloproliferative neoplasms in Japan

2014/10/31

Detail

Poster presentation

Establishment of a novel assay for MPLW515L/K detection and allele burden measurement

2014/10/31

Detail

Oral presentation(general)

Selective Enrichment of Uncultured Ammonia-Oxidizing Bacteria and Archaea and Nitrite-0xidizing Bacteria from Freshwater

15th International Symposium on Microbial Ecology2014/08/25

Detail

Poster presentation

Detection of nxrB Genes by in situ RCA-FISH to Identify Unrecognized Nitrite-Oxidizing Microorganisms in Environments

15th International Symposium on Microbial Ecology2014/08/25

Detail

Poster presentation

Selective Inoculation of Microcolonies Materializes Cultivation and Isolation of Previously Uncultured Nitrifying Bacteria

15th International Symposium on Microbial Ecology2014/08/25

Detail

Poster presentation

Selective Enrichment of Ammonia-Oxidizing Archaea and Bacteria from Freshwater

Water Environment and Technology Conference 20142014/06/29

Detail

Poster presentation

The MPLW515L/K Simultaneous Detection/Quantitation Method That Is Tractable to the Massivly Parallel Sequencing

19th Congress of the European Hematology Association2014/06/12

Detail

Poster presentation

The Role of Gut Microbiota in the Progression of Non-Alcohol Fatty Liver Disease

2013/12/13

Detail

Poster presentation

The Regulation of the Intestinal Epithelial Tight Junction Protein by Bifidobacterium bifidum

2013/12/13

Detail

Poster presentation

Establishment of a Newly Developed Culture Method Providing Efficient Oxygen Supply to Biological Systems

Functional Analysis and Screening Technologies Conference2013/10/29

Detail

Poster presentation

A highly sensitive and reliable JAK2V617F detection method for the diagnostics of MPN

2013/10/11

Detail

Poster presentation

Identification and biochemical analysis of cholesterol sulfate and halisulfate 3 as novel hepatitis C virus NS3 helicase inhibitors from marine natural products

HCV meeting 20132013/10/08

Detail

Poster presentation

In situ Identification of Persisters by Visualizing Z-Ring Formation

Euro Biofilms 20132013/09/10

Detail

Oral presentation(general)

High-Rate Nitrification of Electronic Industry Wastewater by Using Nitrifying Granules

3rd International Conference on Nitrification2013/09/04

Detail

Poster presentation

Comparative Physiological Analysis of Nitrospira Strains Isolated from a Wastewater Treatment Plant

3rd International Conference on Nitrification2013/09/04

Detail

Poster presentation

Molecular Analysis of Enrichment Cultures of Freshwater Anammox Bacteria

3rd International Conference on Nitrification2013/09/04

Detail

Poster presentation

An Innovative Cultivation and Isolation Strategy for Uncultured Nitrifying Bacteria

3rd International Conference on Nitrification2013/09/03

Detail

Oral presentation(general)

Applicability of a Sequencing Batch Membrane Biofilm Reactor for Simultaneous Nitrogen and Phosphorus Removal from Low C/N Ratio Wastewater

Water Environment and Technology Conference 20132013/06/15

Detail

Poster presentation

The Interaction between Commensal Bacteria and Innate Immune Receptors for Modulating the Intestinal Inflammatory Response

Cell Symposia: Microbiome and Host Health2013/05/13

Detail

Poster presentation

An Innovative Cultivation and Isolation Strategy for Uncultured Nitrifying Bacteria

CER International workshop on Biogeochemical cycling and Microbial Ecology for Young Scientists2013/03/19

Detail

Oral presentation(general)

Repairing of TNF-α Induced Intestinal Epithelial TJ Dysfunction by Bifidobacterium bifidum Isolated from Normal Individual

2012/12/07

Detail

Poster presentation

Vascularized Tissue Fabrication from Human Cells Using in vitro Bioreactor

TERMIS-WC 20122012/09

Detail

Poster presentation

Metabolic Change of Cultured Cell Sheets Depending on Oxygen Supply

TERMIS-WC 20122012/09

Detail

Poster presentation

Measuring Energy Metabolism of Cell Sheets Used for Tissue Engineering

2012 International Symposium of Materials on Regenerative Medicine2012/09

Detail

Poster presentation

Computational Modeling of Microbial Granulation Processes for Wastewater Treatment

14th International Symposium on Microbial Ecology2012/08

Detail

Poster presentation

Host-microbes Interplay Involved in the Resolution of Intestinal Inflammation

14th International Symposium on Microbial Ecology2012/08

Detail

Poster presentation

Comparative Physiological Analysis of Previously Uncultured Nitrospira Strains Isolated

14th International Symposium on Microbial Ecology2012/08

Detail

Poster presentation

Selective Cultivation of Nitrifying Bacteria Present in Drinking Water Treatment Plants

14th International Symposium on Microbial Ecology2012/08

Detail

Poster presentation

Novel Method for Cultivation of Uncultured Nitrifying Bacteria

14th International Symposium on Microbial Ecology2012/08

Detail

Oral presentation(general)

Clinical Relevance of JAK2 V617F Somatic Point Mutation in Japanese Myeloproliferative Neoplasm Patients

3rd JSH International Symposium 20122012/05

Detail

Poster presentation

Inhibition of Hepatitis C Virus Replication and Viral Helicase by Ethyl Acetate Extract of the Marine Feather Star Alloeocomatella polycladia

25th International Conference on Antiviral Research2012/04

Detail

Poster presentation

A Feasibility Study on Monitoring the State of Culturing Myocardial Tissues by the Glucose Metabolism Measurement

TERMIS-NA 20112012/12

Detail

Poster presentation

Induction of Mucosal Immune Responses via M cells within Nasal-Associated Lymphoid Tissue

2011/11

Fluorogenic Probe for Detection of mRNA and Intron Lariat RNA

2011/11

Detail

Poster presentation

Nitrogen Treatment by Direct Purification Technology Using Molded Zeolite and Aquatic Plants in an Actual Stream

The 4th IWA-ASPIRE Conference2011/10

Detail

Oral presentation(general)

Quantitative Detection of Cryptosporidium Oocyst in Water Source by Alternatively Binding Probe Competitive Polymerase Chain Reaction (ABC-PCR)

16th International Symposium on Health-Related Water Microbiology2011/09

Detail

Oral presentation(general)

Microbial Population Dynamics during the Formation of Nitrifying Granules and Protein Putatively Relevant for Granulation

IUMS20112011/09

Detail

Poster presentation

In vitro Screening of Prospective Bacteria Modulating the Epithelial Inflammatory Response

IUMS20112011/09

Detail

Poster presentation

Characterization of Colonic Microflora and Innate Immune-Stimulatory Activity during Colitis Progression

IUMS20112011/09

Detail

Poster presentation

In Vitro Construction of Human Vascular Bed for 3-D Tissue Fabrication

TERMIS AP2011/08

Detail

Poster presentation

Development of a Computational Model for the Growth Process of Microbial Granules

Biochemical and Molecular Engineering XVII2011/06

Detail

Poster presentation

High-Throughput in situ Cultivation for Accessing Uncultivable Environmental Microorganisms

Biochemical and Molecular Engineering XVII2011/06

Detail

Poster presentation

The Potential of Stem Cells in Adult Tissues Representative of the Three Germ Layers

TERMIS NA2010/12

Detail

Poster presentation

Alternately Binding Quenching Probe Competitive PCR: Simple, Sensitive, and Cost-Effective JAK2 Allele Burden Quantification Method

52nd ASH Annual Meeting and Exposition2010/12

Detail

Poster presentation

High-Throughput in situ Cultivation - Accessing to Uncultivable Environmental Microorganisms

2010/11

Detail

Poster presentation

Development of in vivo cultivation method targeting human oral microbes

2010/11

Detail

Poster presentation

Growth Characteristics of Uncultured Nitrite-Oxidizing Nitrospira

2010/11

Detail

Poster presentation

Selective and Highly Efficient Enrichment of Uncultured Nitrospira

13th International Symposium on Microbial Ecology2010/08

Detail

Poster presentation

High Throughput in situ Cultivation of Environmental Microorganisms

13th International Symposium on Microbial Ecology2010/08

Detail

Poster presentation

Significance of Reactor Type for High-Rate Nitrification Using Aerobic Granular Sludge

Water Environment and Technology Conference 20102010/06

Detail

Poster presentation

Enhancing Nitrogen and Phosphorus Removal in an Anaerobic/Oxic/Anoxic Sequencing Batch Reactor: The Dependence of the Amount of External Carbon

Water Environment and Technology Conference 20102010/06

Detail

Poster presentation

Nitrogen Removal Performance of Heterotrophic Denitrifying Bacteria Entrapped in PEG Gel Carriers

Water Environment and Technology Conference 20102010/06

Detail

Poster presentation

Formation of Aerobic Granular Sludge in a Continuous Flow Reactor - Control Strategy for Selection of Well-Settling Granular Sludge-

Water Environment and Technology Conference 20102010/06

Detail

Poster presentation

Evaluation of gut environmental dynamics based on gene expression profiling

2nd TNO Beneficial Microbes Conference2010/03

Detail

Poster presentation

Monitoring of metabolic dynamics in microbial ecosystems using a combination of DNA fingerprinting and metabolome analysis based on stable isotope labeling

2nd TNO Beneficial Microbes Conference2010/03

Detail

Poster presentation

High Throughput in situ Cultivation as a New Method for Cultivation of Uncultivable Environmental Microorganisms

Asia Pacific Biochemical Engineering Conference (APBioChEC'09)2009/11

Detail

Poster presentation

Photoactivation of Caged Fluorescent Oligodeoxynucleotide in Cell

Joint Symposium of 5th Annual Meeting of Oligonucleotide Therapeutics Society and the 19th Antisense Symposium2009/11

Detail

Poster presentation

High Rate Nitrification Using Aerobic Granular Sludge

6th Netherlands-Japan Workshop on Water Technology2009/10

Detail

Oral presentation(general)

Subcutaneous Transplantation of Oral Muscosal Epithelial Cell Sheets Fabricated on Temperature-Responsive Culture Dishes

TERMIS 2nd World Congress2009/09

Detail

Poster presentation

Inflammation Analyses after Subcutaneous Transplantation of Cell Sheets for a Novel Tissue Engineering Method

TERMIS 2nd World Congress2009/09

Detail

Poster presentation

Significance of Reactor Type for High-Rate Nitrification Using Aerobic Granular Sludge

IWA 2nd Specialized Conference on Nutrient Management in Wastewater Treatment Process2009/09

Detail

Oral presentation(general)

Reduction-Triggered Fluorescence Amplification Probe for the Detection of Intracellular RNAs

42nd IUPAC Congress2009/08

Detail

Poster presentation

Microscopic and Macroscopic Models for Aerobic Granular Sludge Processes

Microbial Population Dynamics in Biological Wastewater Treatment (ASPD5)2009/05

Detail

Poster presentation

New Methodologies for Isolation and Cultivation of Uncultivable Microorganisms in Wastewater Treatment Processes

Microbial Population Dynamics in Biological Wastewater Treatment (ASPD5)2009/05

Detail

Poster presentation

Microbial Population Dynamics and Community Structure in Nitrifying Granules Analyzed by Experimental Approach and Two-Dimensional Biofilm Model

Biofilm III2008/10

Detail

Oral presentation(general)

Systems microbial ecology to understand mechanisms of nitrifying granuleformation

14th Symposium of Young Asia Biochemical Engineers Community2008/10

Detail

Poster presentation

Reduction-Triggered Fluorescence Probe Amplifying Nucleic Acids Signal for the Detection of Intracellular RNAs

14th Symposium of Young Asia Biochemical Engineers Community2008/10

Detail

Poster presentation

Fluorogenic Probe Triggered by Reduction for Nucleic Acids Sensing

35th International Symposium on Nucleic Acids Chemistry2008/09

Detail

Oral presentation(general)

High-throughput Screening Assay for Hepatitis C Virus Helicase Inhibitors Using Fluorescence-Quenching Phenomenon

Japan-China Medical Workshop on Drug Discoveries and Therapeutics 20082008/09

Detail

Oral presentation(general)

New Cutivation Method for Microorganisms Requiring Environment Simulated Conditions

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Microbial Cell Chromatography (MCC) as a New Tool for Concentration of Specific Microbial Cell from Environment

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Cultivation of Uncultured Nitrite-Oxidizing Bacteria

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Sequence-Based Approach to Isolate Novel Lipases from Environmental DNA Using pre-Amplified Inverse PCR

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Systems Microbial Ecology to Understand Microbial Community of Nitrifying Granules

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Quantification of the Genus Dehalococcoides Using Alternately Binding Probe Competitive PCR (ABC-PCR)

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Characterization of Active Denitrifying Bacteria by 15N-DNA-Stable Isotope Probing

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Alternately Binding Probe Competitive Polymerase Chain Reaction for Quantification of the gene Encoding Ammonia Monooxygenase

12th International Symposium on Microbial Ecology2008/08

Detail

Poster presentation

Flurorescence Quenching-Based Assay for Monitoring the RNA Helicase Activity of Hepatitis C Virus NS3

13th Annual Meeting of the RNA Society (RNA 2008)2008/07

Detail

Poster presentation

New Methodologies for Isolation and Cultivation of Unculturable Microorganisms: Hollow Fiber Membrane Chamber and Microbial Cell Chromatography

ASM 108th General Meeting2008/06

Detail

Poster presentation

Phylogenetic Diversity and Abundance of Ammonia-Oxidizing Archaea and Bacteria in Aquarium Biofiltration Systems

ASM 108th General Meeting2008/06

Detail

Poster presentation

Molecular Diversity of Bacterial Chitinases in Arable Soils and Effects of Environmental Factors on Chitinolytic Bacterial Community

ASM 108th General Meeting2008/06

Detail

Poster presentation

Fluorescence Detection of Nucleic Acids Triggered by DNA-Templated Chemical Reaction

235th ACS National Meeting2008/04

Detail

Poster presentation

Real-Time Control Strategy for Simultaneous Nitrogen and Phosphorus Removal Using Aerobic Granular Sludge

IWA 4th SBR Conference2008/04

Detail

Oral presentation(general)

Tertiary Treatment of Domestic Wastewater Using Zeolite Ceramics and Aquatic Plants

8th Specialized Conference on Small Water and Wastewater Systems2008/02

Detail

Oral presentation(general)

Challenge for Formation of Aerobic Granular Sludge in a Continuous Flow Reactor

IWA Biofilm Technologies Conference2008/01

Detail

Oral presentation(general)

Assessment of the Biofilm Model Implementing Kinetic Parameters Estimated by Respirometric Technique through Comparison with Experimental Data

IWA Biofilm Technologies Conference2008/01

Detail

Poster presentation

Sequencing Batch Membrane Biofilm Reactor for Simultaneous Nitrogen and Phosphorus Removal: Effect of Membrane Biofilm and Applicability to Low CON/N Wastewater

BioMicroWorld 20072007/11

Detail

Poster presentation

Systems Microbiological Approach to Understand Biofilms

13th Symposium of Young Asia Biochemical Engineers Community2007/10

Detail

Poster presentation

Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier

2nd IWA ASPIRE (Asia Pacific Regional Group) Conference2007/10

Detail

Oral presentation(general)

Nitrogen Removal Performance Using Anaerobic Ammonium Oxidation at Low Temperatures

2nd IWA ASPIRE (Asia Pacific Regional Group) Conference2007/10

Detail

Poster presentation

Characterization of the Microbial Community in the Anaerobic/Oxic/Anoxic Process without Excess Sludge Production

2nd IWA ASPIRE (Asia Pacific Regional Group) Conference2007/10

Detail

Poster presentation

Fluorescence Activation System Triggered by Chemical Reaction on Nucleic Acids

The 2nd International Workshop on Approaches to Single-Cell Analysis2007/09

Detail

Poster presentation

Development of New Methodologies for Cultivating Environmental Microorganisms

ISME Asia 20072007/09

Detail

Poster presentation

Identification of Bacterial Population Involved in the Methane Oxidation-Coupled Nitrate Depletion under Oxygen-Limited Conditions by Using Stable-Isotope Probing

ASM 107th General Meeting2007/05

Detail

Poster presentation

New Probes and Autofluorescence Observation that Distinguish aach Subgroup of Defluvicoccus-Relative G-Bacteria in a Wastewater Treatment Process

ASM 107th General Meeting2007/05

Detail

Poster presentation

Pre-Amplification Technique of Flanking Region for Isolating Entire Gene from Environmental DNA by Inverse PCR

ASM 107th General Meeting2007/05

Detail

Poster presentation

Subcutaneous Transplantation of Autologous Oral Muscosal Epithelial Cell Sheets Fabricated on Temperature-Responsive Culture Dishes

Society of Biomaterials 2007 Annual Meeting2007/04

Detail

Oral presentation(general)

Community Structure of Nitrifying and Heterotrophic Bacteria in Autotrophic Nitrifying Granules as Characterized by Experimental and Simulation Analysis

ASM Conferences Biofilms 20072007/03

Detail

Poster presentation

Quantitative Detection of 16S rRNA in a Microbial Community Using RNA Microarray

12th Symposium of Young Asia Biochemical Engineers Community2006/11

Detail

Oral presentation(general)

Combined Approach for Characterization of Community Structure in Nitrifying Granules as Evaluated by Experimental and 2-Dimensional Biofilm Model Analyses

2nd Workshop on Aerobic Granular Sludge2006/09

Detail

Oral presentation(general)

Simultaneous Nitrogen and Phosphorus Removal from High Strength Industrial Wastewater Using Aerobic Granular Sludge

2nd Workshop on Aerobic Granular Sludge2006/09

Detail

Oral presentation(general)

Experimental and Simulation Analysis of Community Structure of Nitrifying Bacteria in a Membrane-Aerated Biofilm

IWA Biofilm Systems VI2006/09

Detail

Oral presentation(general)

Redox-Stratification Controlled Biofilm for Completely Autotrophic Nitrogen Removal: Modeling the Effect of Substrate Co- versus Counter-Diffusion on Performance

IWA Biofilm Systems VI2006/09

Detail

Oral presentation(general)

Hollow Fiber Membrane Chamber System as a High-Performance Method for Isolation and Cultivation of Environmental Microorganisms

11th International Symposium on Microbial Ecology2006/08

Detail

Oral presentation(general)

DNA Microarray-Transcriptional Profiling of Nitrosomonas europaea in Response to Linear Alkylbenzene Sulfonates

11th International Symposium on Microbial Ecology2006/08

Detail

Poster presentation

Isolation of Novel Lipase Gene from Environmental DNA Using Pre-amplified Inverse PCR

11th International Symposium on Microbial Ecology2006/08

Detail

Poster presentation

Method to Prepare the Template DNA for Inverse PCR, and Its Application in Isolation of Novel Gene from Environmental DNA

11th International Symposium on Microbial Ecology2006/08

Detail

Poster presentation

Optimization of the Cell Wall Permealizing Conditions for Highly Sensitive Fluorescence in situ Hybridization

11th International Symposium on Microbial Ecology2006/08

Detail

Poster presentation

Use of Stable-Isotope Probing Approach to Identify Active Bacteria in Methane-Dependent Denitrifying Consortia

11th International Symposium on Microbial Ecology2006/08

Detail

Poster presentation

A Novel Quantitative Competitive LAMP with an Alternatively Binding Probe for Quantification of the Gene Encoding Ammonia Monooxygenase

11th International Symposium on Microbial Ecology2006/08

Detail

Poster presentation

Microbial Community and Growth Characteristic of Bacteria Exhibiting a High Anaerobic Ammonium Oxidation Activity

International Symposium on Environmental Biotechnology 20062006/07

Detail

Poster presentation

Identification of Denitrifying Polyphosphate-Accumulating Organisms Based on their Functions by Flow Cytometric Sorting

International Symposium on Environmental Biotechnology 20062006/07

Detail

Poster presentation

Characterization of the High-Density Compounds Containing Organisms in Enhanced Biological Phosphorus Removal Process

International Symposium on Environmental Biotechnology 20062006/07

Detail

Poster presentation

Effects of Composition and Injection Stage of Additional Substances on Nutrient Removal Efficiency of the Anaerobic/Aerobic/Anoxic Process

International Symposium on Environmental Biotechnology 20062006/07

Detail

Poster presentation

Community Analysis of Denitrifying Bacteria Responsible for High-Rate Denitrification in High-Salinity Wastewater System

ASM 106th General Meeting2006/05

Detail

Poster presentation

Positively Charged Surface is a Determining Factor on Nascent Bacterial Adhesion and Subsequent Biofilm Formation

Biofilms II -Attachment and Detachment in Pure and Mixed Cultures- 2006/03

Detail

Poster presentation

Experimental Support for the Individual-Based Modeling of a Membrane-Aerated Biofilm

Biofilms II -Attachment and Detachment in Pure and Mixed Cultures- 2006/03

Detail

Oral presentation(general)

Use of Real-Time PCR to Examine the Relationship between Ammonia Oxidizing Bacterial Populations and Nitrogen Removal Efficiency in a Small Decentralized Treatment System "Johkasou"

IWA 7th Specialised Conference on Small Water and Wastewater Treatment Systems2006/03

Detail

Oral presentation(general)

Anaerobic/Oxic/Anoxic Granular Sludge Process as an Effective Nutrient Removal Process Utilizing Denitrifying Polyphosphate-Accumulating Organisms

China/USA/Japan Koint Chemical Engineering Conference2005/10

Detail

Oral presentation(general)

Population Dynamics of Ammonia Oxidizing Bacteria in Full-Scale Advanced Johkasou Using Real-Time PCR Assay

IWA Specialized Conference on Nutrient Management in Wastewater Treatment Processes and Recycle Streams2005/09

Detail

Poster presentation

Oxidation of Chlorinated Volatile Organic Compounds in a Bubble Column Photochemical Reactor. Determination of the Optical Path Length in Two Reactor Configurations.

IOA 17th World Ozone Congress2005/08

Detail

Poster presentation

Development of a New Detection Technique of Specific Gene by Affinity Capillary Electrophoresis

19th International Symposium on Microscale Bioseparation2005/07

Detail

Poster presentation

Evaluation of Sludge Reduction and Phosphorus Recovery Efficiencies in a New Advanced Wastewater Treatment System Using Denitrifying Polyphosphate Accumulating Organisms

1st IWA ASPIRE (Asia Pacific Regional Group) Conference2005/07

Detail

Oral presentation(general)

Identification of Polyphosphate-Accumulating Organisms and Glycogen-Accumulating Organisms in Enhanced Biological Phosphorus Removal by Using Density Separation and Molecular Techniques

1st IWA ASPIRE (Asia Pacific Regional Group) Conference2005/07

Detail

Oral presentation(general)

Real-Time LAMP (Loop-Mediated Isothermal Amplification of DNA) as a Novel and Simple Method for Quantitative Monitoring of Environmental Microorganisms

4th IWA Activated Sludge Population Dynamics Specialist Conference2005/07

Detail

Oral presentation(general)

Nitrite Reductase (nirS) Genes in an Enhanced Biological Phosphorus Removal Process

4th IWA Activated Sludge Population Dynamics Specialist Conference2005/07

Detail

Poster presentation

Microbial Community of Denitrifying Bacteria for High-Rate Denitrification of Saline Industrial Wastewater

4th IWA Activated Sludge Population Dynamics Specialist Conference2005/07

Detail

Poster presentation

Microbial Community Analysis of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria in a Continuous-Feeding Cultivation System

4th IWA Activated Sludge Population Dynamics Specialist Conference2005/07

Detail

Poster presentation

Characterization of Active Members in EBPR Process by Using MAR-FISH Combined with Buoyant Density Separation

4th IWA Activated Sludge Population Dynamics Specialist Conference2005/07

Detail

Poster presentation

Identification of Denitrifying Population in Activated sludge by Stable-Isotope Probing

The 8th Symposium on Bacterial Genetics and Ecology2005/06

Detail

Poster presentation

Monitoring the Microbial Population Dynamics at the Start-Up Stage of Wastewater Treatment Reactor Using Terminal Restriction Fragment Length Polymorphism

The 8th Symposium on Bacterial Genetics and Ecology2005/06

Detail

Poster presentation

Multidimensional Scaling Analysis of the Succession of a Microbial Community Related to Performance of a Wastewater Treatment Process

The 8th Symposium on Bacterial Genetics and Ecology2005/06

Detail

Poster presentation

Oxidation of Chlorinated Volatile Organic Compounds (CVOCs) in a Bubble Column Photochemical Reactor: Effect of CVOCs on Mass Transfer and Reaction Mechanism

5th International Symposium on Catalysis in Multiphase Reactors2005/06

Detail

Oral presentation(general)

High-Rate Nitrification Using Aerobic Granular Sludge

3rd IWA Leading-Edge Technology Conference2005/06

Detail

Oral presentation(general)

Oxidation of Chlorinated Volatile Organic Compounds (CVOCs) in a Bubble Column Photochemical Reactor

10th Asian Pacific Confederation of Chemical Engineering (APCChE 2004)2004/10

Detail

Oral presentation(general)

Enzymatic Reaction in Organic Solvent Using Immobilized Lipase on the Polymer Brush

10th Asian Pacific Confederation of Chemical Engineering (APCChE 2004)2004/10

Detail

Oral presentation(general)

Antimony Binding to Polyol Brush onto the Porous Membrane

10th Asian Pacific Confederation of Chemical Engineering (APCChE 2004)2004/10

Detail

Oral presentation(general)

Feasibility study of a membrane-aerated biofilm reactor to achieve controllable nitrification under oxygen depleted conditions

4th IWA World Water Congress2004/09

Detail

Oral presentation(general)

Effect of Ammonium Concentration and Temperature on Transcriptional Activity of Ammonia Monooxygenase Gene in Ammonia Oxidizer

10th International Symposium on Microbial Ecology2004/08

Detail

Poster presentation

Molecular Diversity of Nitrite Reductase Genes (nirK and nirS) in the Denitrification Reactor of Saline Wastewater Treatment

10th International Symposium on Microbial Ecology2004/08

Detail

Poster presentation

Monitoring Complex Bacterial Communities using Terminal Restriction Length Polymorphism: Application to Wastewater Treatment Reactor

10th International Symposium on Microbial Ecology2004/08

Detail

Poster presentation

Loop-Mediated Isothermal Amplification (LAMP) of DNA as a New Method for Quantitative Monitoring of Environmental Bacteria and Their Gene Expression

10th International Symposium on Microbial Ecology2004/08

Detail

Poster presentation

Identification of Active Denitrifying Population in Activated Sludge Using Stable-Isotope-Probing (SIP)

10th International Symposium on Microbial Ecology2004/08

Detail

Poster presentation

Oxidation of chlorinated volatile organic compounds (CVOCs) in a bubble column photochemical reactor. In search for a cost-effective reactor design.

16th International Congress of Chemical and Process Engineering (CHISA 2004)2004/08

Detail

Oral presentation(general)

Quantitative monitoring of environmental bacteria and their gene expression by using loop-mediated isothermal amplification (LAMP)

ASM 104th General Meeting2004/05

Detail

Poster presentation

Oxidation of CVOCs in a UV-Bubble Column Photochemical Reactor Applying the UV/H2O2 Technique

Regional Symposium on Chemical Engineering 20032003/12

Detail

Oral presentation(general)

Simulation of Competition within AOB in Biofilms Using a Pseudo-3D CA-Hybrid Model

ASM Conferences Biofilm 20032003/11

Detail

Poster presentation

Microbial Community Analysis of Toxic Cyanobacteria in Water Environment by Molecular Genetic Tools

2nd IWA Asia-Pacific Regional Conference2003/10

Detail

Oral presentation(general)

Cultivation-Dependent and -Independent Analysis of Bacterial Community in Aquatic Model Ecosystems

2nd IWA Asia-Pacific Regional Conference2003/10

Detail

Poster presentation

Introducing Radioactive Substrate to the Microcosm Systems to Study Effects of Nitrogenous Compounds Dosage on the Microbial Population and Material Flux in Aquatic Ecosystem

2nd IWA Asia-Pacific Regional Conference2003/10

Detail

Poster presentation

Characterization of Denitrifying Bacteria in Saline and Nitrate Containing Industrial Wastewater Treatment System Based on 16S rRNA and Nitrite Reductase Gene

ASM 103rd General Meeting2003/05

Detail

Poster presentation

In situ Detection of Ammonia Monooxygenase Gene in a Starved Biofilm

ASM 103rd General Meeting2003/05

Detail

Poster presentation

Novel Method for Monitoring of Environmental Bacteria by Using Loop-Mediated Isothermal Amplification (LAMP) of DNA

ASM 103rd General Meeting2003/05

Detail

Poster presentation

Development of Membrane-Aerated Biofilm Reactor Applicable to Ammonia Removal from Industrial Wastewater

2nd Joint China/Japan Chemical Engineering Symposium2002/11

Detail

Oral presentation(general)

A Novel Bioprocess for the Treatment of Heavy Metal Contaminated Water Using Fibrous Solid Wastes as a Bio-carrier

52nd Canadian Chemical Engineering Conference2002/10

Detail

Oral presentation(general)

Kinetic Considerations of UV-bubble Column Reactor for Removal of CVOCs from Gas Phase

15th Int. Congress of Chemcal and Process Engineering (CHISA2002)2002/08

Detail

Oral presentation(general)

RT-PCR DGGE for Monitoring Gene Expression of Ammonia Oxidizer in Wastewater Treatment Process

The 7th Symposium on Bacterial Genetics and Ecology2002/06

Detail

Poster presentation

In Situ PCR for Detection of Ammonia Monooxygenase Gene in a Biofilm

The 7th Symposium on Bacterial Genetics and Ecology2002/06

Detail

Poster presentation

Characterization of Nitrite Reductase Activity Based on Nitrite Reductase Genes (nirS) in Saline Watewater Treatment Processes

The 7th Symposium on Bacterial Genetics and Ecology2002/06

Detail

Poster presentation

Quantitative Analysis of Microbial Community in the Denitrification Process of Saline Wastewater Treatment System Using New 16S rRNA- Targeted Oligonucleotide Probes

The 7th Symposium on Bacterial Genetics and Ecology2002/06

Detail

Poster presentation

Separation of ultrafine CdS particles from low concentrated cadmium wastewater - a novel treatment method of heavy metal contaminated wastewater based on surface characteristics -

Interfaces Against Pollution (IAP2002)2002/05

Detail

Oral presentation(general)

Rapid immobilization of bacterial cells to fibrous collector by heterocoagulation phenomenon and its application to the biological wastewater treatment process

Interfaces Against Pollution (IAP2002)2002/05

Detail

Oral presentation(general)

Comparison of Detection Specificity of Nitrifying Bacteria in Biofilm Using Fish and in Situ Fluorescence Antibody Method

International Specilaized Conference on Biofilm Monitoring2002/03

Detail

Poster presentation

Monitoring of Dynamic Transition of Bacterial Community Structure in a Nitrifying Biofilm

Int. Specialized Conference on Biofilm Monitoring2002/03

Detail

Poster presentation

In Situ PCR for Visualizing of Distribution of a Functional Gene "amoA" in Biofilm Regardless of its Activity

Int. Specialized Conference on Biofilm Monitoring2002/03

Detail

Oral presentation(general)

Recovery of Antimony from Polyester Textile Wastewater Using Chelating Porous Membrane

51st Canadian Chemical Engineering Conference2001/10

Detail

Oral presentation(general)

Kinetics of Tetrachloroethylene (PCE) Gas Degradation and Byproducts Formation during UV/H2O2 Treatment in UV-buble Column Reactor

6th World Congress of Chemical Engineering2001/09

Detail

Oral presentation(general)

Ecological Structure Analysis of Nitrifying Bacteria in Various Types Wastewater Treatment Process Using Molecular Biological Techniques

5th International Symposium on Environmental Biotechnology2001/09

Detail

Poster presentation

Quantitative Analysis of amoA Expression in Autotrophic Ammonia-Oxidizing Bacteria by Competitive Reverse Transcription-PCR

9th International Symposium on Microbial Ecology2001/08

Detail

Poster presentation

Characterization of Microbial Community in Nitrogen Removal Process of Metallurgic Wastewater by Cultivation and Non-Cultivation Methods

9th International Symposium on Microbial Ecology2001/08

Detail

Poster presentation

Direct Detection of Ammonia Monooxygenase Gene in a Biofilm by in situ PCR

9th International Symposium on Microbial Ecology2001/08

Detail

Poster presentation

Analysis of mRNA as an Indicator of Ammonia-Oxidizing Activity Responding to the Environmental Condition

9th International Symposium on Microbial Ecology2001/08

Detail

Poster presentation

Detection and Quantification of Expression of amoA by Competitive Reverse Transcription PCR

3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes2001/06

Detail

Oral presentation(general)

PCR-DGGE Analysis of Denitrifying Bacteria in a Metallurgic Wastewater Treatment Process

3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes2001/06

Detail

Poster presentation

Real-Time Monitoring of Ammonia-Oxidizing Activity in a Nitrifying Biofilm by amoA mRNA Analysis

3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes2001/06

Detail

Poster presentation

Microbial Community Analysis of a Novel Biological Nutrient Removal Process Configured with Anaerobic/Aerobic/Anoxic Conditions

3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes2001/06

Detail

Poster presentation

Simultaneous Nitrification and Denitirification in a Single Reactor Using Membrane Aerated Biofilm Reactor

Regional Symposium on Chemical Engineering 20002000/12

Detail

Oral presentation(general)

Influence of H2O2 Concentration and Gas Flow Rate on the Photodegradation of Tetrachloroethylene in UV-Bubble Column Reactor

Advanced Study Institute on Remediation of the Aquatic and Atmospheric Environments by Advanced Oxidation2000/11

Detail

Poster presentation

Enhancement of Nitrifying Biofilm Formation Using Selected EPS Produced by Heterotrophic Bacteria

IWA Specialist Conference on EPS2000/09

Detail

Oral presentation(general)

Efficient TOC Removal of Wastewater Containing 1,2-Naphthoquinone-2-diazido-5-sulfonic Acid Sodium Salt with H2O2/UV in a Batch Reactor

第1回日中化学工学シンポジウム2000/09

Detail

Oral presentation(general)

Oxidation of 1,2-Naphthoquinone-2-Diazido-5-Sulfonic Acid Sodium Salt in Aqueous Media by H2O2/UV Process

1st Joint China/Japan Chemical Engineering Symposium2000/09

Detail

Oral presentation(general)

Oxidation of 1,2-Naphthoquinone-2-Diazido-5-Sulfonic Acid Sodium Salt in Synthetic Wastewater and in Raw Wastewater by UV/H2O2 Process

IWA Specialist Conference on Pollution Control and Reclamation in Process Industries2000/09

Detail

Poster presentation

Biological Nitrogen Removal from Industrial Wastewater Discharged from Metal Recovery Processes

1st World Congress of the International Water Association2000/07

Detail

Oral presentation(general)

Application of PCR-DGGE Technique to Analysis of Microbial Population in Metallurgic Wastewater Treatment System

5th International Symposium on Environmental Biotechnology2000/07

Detail

Poster presentation

Population Dynamics of Nitrifying Bacteria in Compact Gappei-JOHKASO Investigated by Fluorescent in situ Hybridization

5th International Symposium on Environmental Biotechnology2000/07

Detail

Poster presentation

Characteristics of Denitrifying Phosphate-Accumulating Organisms Cultivated in Anaerobic/Aerobic Sequencing Batch Reactor

1st World Congress of the International Water Association2000/07

Detail

Poster presentation

Fluorescence in situ Hibridization (FISH) Analysis of Nitrifying Bacteria in Wastewater Treatment Process Biofilm

Asia-Pacific Biochem. Eng. Conf.1999/11

Detail

Oral presentation(general)

Dynamic Response of the Three-Phase Fluidized Bed Biofilm Reactor for Wastewater Treatment to Step Change in Inlet Concentration

Regional Symposium on Chemical Engineering 19991999/11

Detail

Oral presentation(general)

Enhancement of Nitrifying Biofilm Formation Using Surface Modified Membrane and Evaluation of Its Nitrification Rate for Inorganic Wastewater

Regional Symposium on Chemical Engineering 19991999/11

Detail

Oral presentation(general)

Tailoring of Highly Efficient Nitrifying Biofilms in Fluidized Bed for Ammonia-Rich Industrial Wastewater Treatment

7th IAWQ Asia-Pacific Regional Conference1999/10

Detail

Oral presentation(general)

Kinetics Equation for Simultaneous Oxidation of Total Organic Carbon and Ammonium-Nitrogen in Three-Phase Fluidized Bed Biofilm Reactor

IAWQ Conference on Biofilm Systems1999/10

Detail

Oral presentation(general)

Optimum Design of Anaerobic-Aerobic Bioreactor for Nitrogen Removal from Industrial Wastewater

48th Canadian Chemical Engineering Conference1998/10

Detail

Oral presentation(general)

Direct Observation of Nitrifying Biofilms on Particles by Electron Microscopy

IAWQ International Speciality Conference on Microbial Ecology of Biofilms1998/10

Detail

Poster presentation

Reaction Rate Equations and Kinetic Parameters from Batch Experiments on Three-Phase Fluidized Bed Biofilm Reactor for Wastewater Treatment

Regional Symposium on Chemical Engineering 19981998/10

Detail

Oral presentation(general)

Kinetics of Biological Treatment of Phenolic Wastewater in Three-Phase Fluidized Bed Containing Biofilm and Suspended Sludge

IAWQ 19th Biennial International Conference1998/06

Detail

Oral presentation(general)

Evaluation of Kinetic Parameters from Concentration Step Change Response of Three-Phase Fluidized Bed Biofilm Reactor for Wastewater Treatment

IAWQ 19th Biennial International Conference1998/06

Detail

Oral presentation(general)

Development of High-Performance Biological System with Anaerobic-Aerobic Biofilm for Treatment of Photographic Processing Wastewater

47th Canadian Chemical Engineering Conference1997/10

Detail

Oral presentation(general)

Biological Treatment for Nitrogen Removal from Industrial Wastewater

Regional Symposium on Chemical Engineering 19971997/10

Detail

Oral presentation(general)

Patent

Reference Number:34

生物膜の製造方法およびそれを用いた無機性アンモニア廃水連続処理装置(日本)

常田 聡, 平田 彰

2000-183029、2002-001389

Reference Number:38

担体を利用した高度処理方法および高度処理装置(日本)

平田 彰, 常田 聡, 小西 功三

2000-297314、2002-102881、3716170

Reference Number:125

硝化グラニュールの形成方法(日本)

常田 聡, 平田 彰

2002- 70011、2003-266095

Reference Number:126

窒素・リン同時除去型排水処理方法(日本)

常田 聡, 平田 彰

2002- 93011、2003-285096、4267860

Reference Number:135

メンブレンバイオリアクタによる窒素除去方法(日本)

常田 聡, 平田 彰

2002-053057、2003-251381

Reference Number:136

硝化・脱窒同時進行型反応構造体及びその製造方法(日本)

平田 彰, 青井 議輝, 常田 聡

2002-264812、2004- 97974

Reference Number:149

廃水処理方法及び廃水処理装置(日本)

常田 聡, 平田 彰

2004-244480、2006- 61764

Reference Number:359

排水処理システム及び排水処理方法(日本)

常田 聡, 平田 彰

2004- 50590、2005-238084

Reference Number:430

排水処理装置(日本)

常田 聡, 平田 彰, 寺田 昭彦

2004-274386、2006- 87990、4609989

Reference Number:439

排水の処理方法および装置(日本)

常田 聡, 平田 彰

2005-144132、2006-320793

Reference Number:714

好気性グラニュールの形成方法、水処理方法及び水処理装置(日本)

常田 聡, 岸田 直裕

2007-236683、2009- 66505、4925208

Reference Number:715

インバースPCR方法に用いる鋳型DNA鎖の生産方法(日本)

常田 聡, 寺原 猛

2007-046046、2007-252373

Reference Number:753

微生物の濃縮精製装置及び濃縮精製方法(日本)

青井 議輝, 常田 聡

2007-238595、2009- 65915

Reference Number:888

微生物の単離培養方法及び培養キット(日本)

青井 議輝, 常田 聡

2008-208790、2010- 41967、5316983

Reference Number:1092

APOBEC3Gの活性測定方法(日本)

常田 聡

2010-144122、2011-115151

Reference Number:1117

JAK2遺伝子の変異解析方法(日本)

常田 聡

2010-174571、2012- 34580、5787304

Research Grants & Projects

Grant-in-aids for Scientific Research Adoption Situation

Research Classification:

Systems engineering approach for colitis remission by use of gut commensal bacteria

2011/-0-2015/-0

Allocation Class:¥17940000

Research Classification:

Development of an enzyme-immobilized membrane mitigating biofouling in a membrane bioreactor

Allocation Class:¥3470000

Research Classification:

Mechanical stimulation-induced vascular elastogenesis

Allocation Class:¥3120000

Research Classification:

Molecular Ecology of Non-Extremophilic Archaea in Deep Ocean

Allocation Class:¥52650000

Research Classification:

Development of High-Performance Wastewater Treatment System Capable of Simultaneous Nitrogen and Phosphorus Removal by Controlling Microbial Ecology

Allocation Class:¥16650000

Research Classification:

Removal of chlorinated organic compounds from ground water by AOP in a bubble column photochemical reactor

Allocation Class:¥3100000

Research Classification:

Development of a membrane-aerated biofilm reactor applicable to livestock wastewater

Allocation Class:¥13200000

Research Classification:

Clarification of Marangoni Convection under microgravity conditions for high quality crystal growth

Allocation Class:¥6500000

Research Classification:

Marangoni convection in the melt during crystal growth of compound semiconductor under Microgravity

Allocation Class:¥2500000

Research Classification:

In vivo cultivating system for oral biofilm bacteria.

2011-2012

Allocation Class:¥3510000

Research Classification:

Molecular mechanism analysis of dormant cells causing refractory nature of bacterial infections

2015/-0-2017/-0

Allocation Class:¥3770000

Research Classification:

Search for antiviral molecules from coral reef organisms

2014/-0-2017/-0

Allocation Class:¥4810000

Research Classification:

Development of an exhaustive detection technique for viable waterborne bacteria by using fluorescence activated cell sorter and next-generation sequencing

2013/-0-2016/-0

Allocation Class:¥17550000

Research Classification:

In vivo cultivating system for oral biofilm bacteria.

2011/-0-2013/-0

Allocation Class:¥3510000

Research Fund Acceptance Situation

Embodiment:Joint research programs

Advanced Treatment of Swine Wastewater by Membrane-Aerated Bio-film System2000-

On-campus Research System

Special Research Project

窒素循環に寄与する未培養微生物を標的とした新規分離培養手法

2011Collaborator:大坂 利文, 青井 議輝, 藤谷 拓嗣

Research Results Outline: 硝化は地球規模の窒素循環や,排水または浄水からの生物学的窒素除去プロセスにおいて重要な反応過程である。しかし硝化に関わる微生物の多くが難培養性である 硝化は地球規模の窒素循環や,排水または浄水からの生物学的窒素除去プロセスにおいて重要な反応過程である。しかし硝化に関わる微生物の多くが難培養性であることから現在までに分離培養されている菌株は少ない。本研究では培養株が特に少ない亜硝酸酸化細菌Nit... 硝化は地球規模の窒素循環や,排水または浄水からの生物学的窒素除去プロセスにおいて重要な反応過程である。しかし硝化に関わる微生物の多くが難培養性であることから現在までに分離培養されている菌株は少ない。本研究では培養株が特に少ない亜硝酸酸化細菌Nitrospiraの獲得を実証モデルとして,3つのステップからなる新規分離培養手法を確立した。 まず,1)下水処理場の活性汚泥をサンプルソースとし亜硝酸塩を含有する無機性の基質を連続的に供給した集積培養槽内でNitrospiraの高度集積化を行った。槽内の亜硝酸濃度を厳密に制御することで系統学的に異なる2種類のNitrospiraを選択的に集積化することが可能であった。つづいて,2)セルソーターを使用し,サンプル中に形成されているマイクロコロニーの大きさと物理的構造の複雑性に基づいて分画することで,Nitrospiraのみから形成されるマイクロコロニー(10-10^2細胞の凝集体)だけを特異的に分取することに成功した。最後に,3)分取したマイクロコロニーをマルチウェルプレートに一つずつ播種し,亜硝酸塩を含有した培地で培養した。その結果,一つのマイクロコロニーからNitrospiraが10^6-10^7 細胞/ mlまで増殖したことを確認できた。培養を行った924サンプルのうち,Sublineaege I に属するNitrospiraの純菌株を5株,Sublineaege IIに属するNitrospiraの純菌株を4株獲得した。これによって,世界で初めて活性汚泥から系統学的に異なった2種類のNitrospiraの分離培養に成功した。さらに,数日間の亜硝酸濃度と硝酸濃度の変化をモニタリングした結果,獲得した2菌種はどちらも亜硝酸酸化活性を持つことが明らかになった。また,電子顕微鏡を用いて獲得した2菌種の観察を行った結果,2菌種とも厚い細胞外ポリマーを持ち,絡み合うようにマイクロコロニーを形成していることがわかった。 硝化に関わる多くの微生物がNitrospiraと同様,環境中でマイクロコロニーを形成することが知られているため,本研究で確立した新規分離培養手法は硝化細菌全般への応用が可能である。

海洋生物抽出液ライブラリーからのC型肝炎ウイルスNS3ヘリカーゼ阻害剤の探索

2012Collaborator:大坂 利文

Research Results Outline: C型肝炎ウイルス(hepatitis C virus,以降HCV)感染者は日本で約200万人と推定されており,感染を放置すると肝硬変・肝癌といった重 C型肝炎ウイルス(hepatitis C virus,以降HCV)感染者は日本で約200万人と推定されており,感染を放置すると肝硬変・肝癌といった重篤な病態に進行する。治療法としてインターフェロン療法が確立されてはいるものの, 難治症例では治療効... C型肝炎ウイルス(hepatitis C virus,以降HCV)感染者は日本で約200万人と推定されており,感染を放置すると肝硬変・肝癌といった重篤な病態に進行する。治療法としてインターフェロン療法が確立されてはいるものの, 難治症例では治療効率が半分程度であること, また副作用が強いことから, より効果的な治療薬の開発が求められている。HCVは約9600塩基からなる1本鎖+鎖RNAをゲノムに有し,ここから前駆体ポリプロテインが翻訳され,最終的に10種類以上のウイルスタンパク質へと成熟する。このうちいくつかのタンパク質を標的にした複製阻害剤が新たな治療薬の候補として開発されつつあり,特にNS3プロテアーゼの阻害剤を従来のインターフェロン療法に加えた併用療法が臨床応用にまで至っている。しかしながら,1種のウイルスタンパク質のみを標的とした治療法では遺伝子変異による薬剤耐性ウイルスの出現が危惧されることから,人類がHCVを克服するためには,複数のウイルスタンパク質を標的とした複数の阻害剤投与を実現することが重要であるといえる。近年,HCVの増殖に関与するNS3がプロテアーゼ活性のみではなく,2本鎖の核酸を1本鎖にほどくヘリカーゼ活性を持ち,このヘリカーゼ活性を失活させることでHCVの増殖を抑えることが可能であることが明らかとなった。 そこで申請者は,独自に構築したNS3ヘリカーゼ活性のハイスループットな活性評価技術を用い,NS3ヘリカーゼ阻害剤の獲得を目的として研究を行った。本測定法は,グアニン塩基との間の光励起電子移動反応によって蛍光が消光する特殊な蛍光色素を用い,ヘリカーゼ活性を簡便かつ迅速に測定することを可能にする。サンプルソースとして創薬資源として実績のある海洋生物抽出物ライブラリーを活用し,陽性試料の探索を行った。獲得した陽性試料はHCVレプリコンシステムを用いてウイルス増殖抑制活性の評価を行い,有望な試料については,活性成分の分画・精製を行い,最終的にNMRを用いて阻害剤の同定を試みた。 海洋生物抽出物668種についてNS3ヘリカーゼ阻害剤の探索を行った結果,117種の試料がヘリカーゼ活性を50%以下に阻害し,さらにその内14種の抽出物にHCV増殖抑制活性を確認した。特に,沖縄産のウミシダAlloecomatella polycladiaの酢酸エチル抽出物に高いHCV増殖抑制活性があることを確認し,同抽出物を分画・精製した結果,極性分画より5種の化合物(その内4種は新規化合物)をNS3ヘリカーゼ阻害剤として同定することに成功した。しかしながら,これら阻害剤はHCV増殖抑制活性を示さなかったため,現在,非極性分画からの阻害剤の探索を引き続き行っている。また,この他にも陽性試料から複数の阻害剤を同定することに成功し,一部の阻害剤については生化学的な解析からNS3へリカーゼへの作用機序を明らかにすることができた。

難培養性アンモニア酸化細菌・古細菌の革新的分離培養戦略

2012Collaborator:大坂 利文

Research Results Outline: 硝化細菌(アンモニア酸化細菌・亜硝酸酸化細菌)は,自然環境における窒素循環,および浄水・排水処理における生物学的窒素除去プロセスを担う重要な細菌であ 硝化細菌(アンモニア酸化細菌・亜硝酸酸化細菌)は,自然環境における窒素循環,および浄水・排水処理における生物学的窒素除去プロセスを担う重要な細菌である。近年の分子生物学的な手法の発展により,硝化細菌は極限・非極限を問わず環境中に普遍的に生息し,系... 硝化細菌(アンモニア酸化細菌・亜硝酸酸化細菌)は,自然環境における窒素循環,および浄水・排水処理における生物学的窒素除去プロセスを担う重要な細菌である。近年の分子生物学的な手法の発展により,硝化細菌は極限・非極限を問わず環境中に普遍的に生息し,系統学的な多様性も高いことが明らかになっている。しかし,高濃度の基質による阻害・遅い増殖速度・強い凝集性を持つために培養が困難であり,硝化細菌に対する有効な培養手法は未だ確立されていない。本研究では,連続流入式の混合型集積培養槽を用いた高度集積培養,およびセルソーターを用いた選択的分取による一連の新規分離培養手法を考案し,特に培養株の少ない貧栄養の淡水環境由来の硝化細菌の獲得を試みた。 培養開始後350日目に集積培養槽から集積サンプル(硝化細菌占有率70%)を採取し,超音波分散処理およびフィルター濾過を行った。セルソーターのパラメーターを細胞の大きさを示す前方散乱光(Forward scatter; FSC)と細胞の内部構造を示す側方散乱光(Side scatter; SSC)に設定し,96ウェルプレート上で1ウェルにつき1つのマイクロコロニーが入るように分注した。FSCを大きくSSCを小さく設定した結果,硝化細菌の形成するマイクロコロニーを選択的に分取することに成功した。一ヶ月間静置培養を行い,増殖が確認されたものに対してシーケンス解析し,菌種を同定した結果,Betaproteobacteria綱に属するアンモニア酸化細菌を10株,Nitrospira綱に属する亜硝酸酸化細菌を15株獲得することに成功した。この中には,16S rRNA遺伝子の相同性が既存株Nitrospira moscoviensisと95%,既存株Nitrosomonas oligotrophaと95%の純菌株も含んでおり,高い新規性が得られた。さらに,淡水環境から亜硝酸酸化細菌Nitrospiraを獲得したのは世界初の快挙である。また,硝化細菌の他にも数種類の純菌株が得られていることから,本手法を応用することで様々な菌の獲得が可能であると期待される。

大腸陰窩における細胞アポトーシス動態の数理モデリング

2013

Research Results Outline: 生体組織中の恒常性維持の仕組みは、癌と深く関係しており、治療手法を考える上で非常に重要である。その理解のために、存在している多数の細胞及びその動的な 生体組織中の恒常性維持の仕組みは、癌と深く関係しており、治療手法を考える上で非常に重要である。その理解のために、存在している多数の細胞及びその動的な振る舞いなど、種々の莫大な要素を含めて考える・計算する必要性がある。そのような作業においては、最適... 生体組織中の恒常性維持の仕組みは、癌と深く関係しており、治療手法を考える上で非常に重要である。その理解のために、存在している多数の細胞及びその動的な振る舞いなど、種々の莫大な要素を含めて考える・計算する必要性がある。そのような作業においては、最適な数理モデルに基づくコンピューターシミュレーションが有効である。この恒常性維持について考える際には、その重要な仕組みである「細胞増殖」と「細胞死」の実装が望ましい。しかし、細胞死の発生確率は健常時では非常に低く、その実装が困難であるためか、既存の数理モデルでは細胞増殖にのみ注目しているものがほとんどであり、細胞死の仕組みについて数理モデルで考察した報告は少ない。そこで本研究では、細胞死の発生が増加する状態、組織中に細胞損傷を引き起こす状態、および誘導刺激が導入された状態に着目した。この数理モデルでは、IBM(Individual-Based Modeling)という、主に生態学で用いられる手法に基づくモデル化が行われており、モデル中の細胞1つ1つに対して設定を行うことで、数多くの細胞から成る組織の振る舞いの模擬を可能にしている。この手法によって、個々の細胞が一律ではなく、それぞれ異なるパラメータに従って動くことが可能になり、各細胞の状態に応じて発生する細胞死を模擬する上で適した手法であると考えられる。また、細胞増殖についてもIBMを用いて模擬することで、おそらく一律ではない各幹細胞の個体差を模擬することができると考えられる。本研究では、恒常性維持に必要な「細胞増殖」と「細胞死」を兼ね備えたモデルを用いて誘導刺激導入時に観測された結果の再現を行った。 本研究では、細胞の入れ替わりが活発に起きている腸上皮組織に着目し、その構造単位である陰窩(いんか)における細胞増殖・分化過程の数理モデル化を試み、組織の恒常性維持機構について定量的な議論を行った。その結果、誘導刺激を受けた際にアポトーシスが誘導される確率やアポトーシスが完了するまでの持続時間が、細胞種ごとに異なるのではないかという予測が立てられた。まず、数理モデルにおいては、陰窩中の細胞種として、自己増殖が可能である幹細胞、幹細胞から分化し一定回数だけ分裂可能であるTA細胞(Transit Amplifying cell、一過性増殖細胞)、TA細胞が一定回数の分裂完了後に分化しそれ以上分裂しない分化細胞の3種類を考えた。なお、TA細胞はその分裂回数ごとに、第1世代から第3世代まで分けて考えた。また、本モデルで仮定した細胞のアポトーシス進行経路では、細胞に誘導刺激(放射線照射を想定)が与えられると、一定確率Pで細胞中に変異が残存し、それを感知して細胞死の誘導が行われる。この数理モデルでシミュレーションを行い、実験から得られたアポトーシス細胞の発生や分布の再現を試みた結果、幹細胞とTA細胞の両方にアポトーシスが起こり、かつPの値がTA細胞の世代ごとに異なることが予測された。具体的には、Pの値はTA細胞第1世代で最も大きく、続いて幹細胞、TA細胞第2世代以降の順に小さくなることが予測された。

環境中のマイクロコロニーを標的とした新規微生物分離培養手法の開発

2014

Research Results Outline:排水処理施設において高度な水処理技術を実現するために,活性汚泥中に生息する微生物の特徴を詳細に把握することは重要である。本研究では,活性汚泥中の微生物排水処理施設において高度な水処理技術を実現するために,活性汚泥中に生息する微生物の特徴を詳細に把握することは重要である。本研究では,活性汚泥中の微生物の系統と凝集性との関係性を明らかにした。また,セルソーターを利用してsingle cellやmic...排水処理施設において高度な水処理技術を実現するために,活性汚泥中に生息する微生物の特徴を詳細に把握することは重要である。本研究では,活性汚泥中の微生物の系統と凝集性との関係性を明らかにした。また,セルソーターを利用してsingle cellやmicrocolonyといった単一菌種の形態を選択的に分取し,排水中の栄養塩除去に寄与する特定微生物の単離・濃縮を目指した。

難治性感染症の原因となる休止細菌を標的とする新規薬剤スクリーニング

2014

Research Results Outline:難治感染症の原因として休止細菌の存在が注目されている。抗生物質は細菌の増殖を標的として細胞を死に至らしめるが,休止細菌は細胞増殖を行わないため抗生物質難治感染症の原因として休止細菌の存在が注目されている。抗生物質は細菌の増殖を標的として細胞を死に至らしめるが,休止細菌は細胞増殖を行わないため抗生物質の作用を受けず,複数種の抗生物質に対して寛容性を示し生存し続ける。本研究では,休止細菌を撲滅できる...難治感染症の原因として休止細菌の存在が注目されている。抗生物質は細菌の増殖を標的として細胞を死に至らしめるが,休止細菌は細胞増殖を行わないため抗生物質の作用を受けず,複数種の抗生物質に対して寛容性を示し生存し続ける。本研究では,休止細菌を撲滅できる新しい薬剤の開発を目指し,我々が開発した世界初の大腸菌の休止細菌マーカー株を利用して,休止細菌の動態をシングルセルレベルで評価できるマイクロ流路を開発した。

「ミニ腸管」形成プロセスにみられる生物学的自己組織化現象の数理学的理解

2014Collaborator:加川友己

Research Results Outline:大腸腫瘍の形態が経時的にどのように形成されるのかを観察することは、従来法の技術では困難であったが,腸オルガノイド培養技術の開発により,ヒト検体における大腸腫瘍の形態が経時的にどのように形成されるのかを観察することは、従来法の技術では困難であったが,腸オルガノイド培養技術の開発により,ヒト検体における腸管上皮の管腔形成異常の動的解析を行うことが可能となった。本研究では,細胞間相互作用の実装が容易な...大腸腫瘍の形態が経時的にどのように形成されるのかを観察することは、従来法の技術では困難であったが,腸オルガノイド培養技術の開発により,ヒト検体における腸管上皮の管腔形成異常の動的解析を行うことが可能となった。本研究では,細胞間相互作用の実装が容易なcellular Potts modeling手法を用いて腸オルガノイド形態形成の2次元数理モデルを構築し,腫瘍性上皮細胞由来オルガノイドにみられる異常な形態が,本質的に何によって引き起こされているのかを明らかにすることを目指した。その結果,細胞分裂時の分裂軸や分裂速度の異常が単一管腔・単層構造の崩れをもたらすことが予想された。

比較解析から迫る亜硝酸酸化細菌Nitrospiraの生態学的ニッチ

2015Collaborator:藤谷拓嗣

Research Results Outline:排水処理場における亜硝酸酸化は,2種類(lineage I,II)のNitrospiraが役割を担っている。本研究では,排水処理場の活性汚泥から獲得し排水処理場における亜硝酸酸化は,2種類(lineage I,II)のNitrospiraが役割を担っている。本研究では,排水処理場の活性汚泥から獲得したND1株(lineage I)とNJ1株(lineage II)の生理学的性質を比較することで実...排水処理場における亜硝酸酸化は,2種類(lineage I,II)のNitrospiraが役割を担っている。本研究では,排水処理場の活性汚泥から獲得したND1株(lineage I)とNJ1株(lineage II)の生理学的性質を比較することで実環境の生態学的ニッチを考察することを目的とした。まず,ND1株は低濃度のアンモニアでも顕著に阻害を受けることがわかった。そこで,両株の競合試験を行ったところ,アンモニアを含まない系ではND1株が,アンモニアを含む系ではNJ1株が優占化した。以上のことから異なるlineage間の生態学的ニッチにおいてアンモニア濃度が重要な因子であることが示唆された。

ゲノム解析から探る亜硝酸酸化細菌Nitrospiraの生理生態

2016Collaborator:藤谷拓嗣

Research Results Outline:亜硝酸酸化細菌であるNitrospira sp. ND1株とNitrospira japonicaNJ1株の比較ゲノム解析を行った。ND1株とNJ1株亜硝酸酸化細菌であるNitrospira sp. ND1株とNitrospira japonicaNJ1株の比較ゲノム解析を行った。ND1株とNJ1株のゲノム配列からコードされている遺伝子を同定した結果、ND1株は4602の候補遺伝子(CDS)を、...亜硝酸酸化細菌であるNitrospira sp. ND1株とNitrospira japonicaNJ1株の比較ゲノム解析を行った。ND1株とNJ1株のゲノム配列からコードされている遺伝子を同定した結果、ND1株は4602の候補遺伝子(CDS)を、NJ1株は4151のCDSを保有していることが明らかとなった。各CDSから両株の代謝経路、微生物構造を予測した結果、TCA回路、解糖系、電子伝達系に関して、2系統の分離株で違いは確認されなかった。一方、運動性に関わる鞭毛合成、微生物間コミュニケーション機能に関わるクオラムセンシング機構、および窒素代謝経路の遺伝子に顕著な違いが確認された。

亜硝酸酸化細菌 Nitrospiraのクオラムセンシング機構の解明

2017Collaborator:藤谷 拓嗣

Research Results Outline:クオラムセンシング(QS)機構は、微生物の細菌密度に依存した活性制御機構である。本研究では、活性汚泥から分離した亜硝酸酸化細菌Nitrospira jクオラムセンシング(QS)機構は、微生物の細菌密度に依存した活性制御機構である。本研究では、活性汚泥から分離した亜硝酸酸化細菌Nitrospira japonica(NJ1株)のQS機構の特性および活性制御メカニズムを調べた。アシル化ホモセリンラク...クオラムセンシング(QS)機構は、微生物の細菌密度に依存した活性制御機構である。本研究では、活性汚泥から分離した亜硝酸酸化細菌Nitrospira japonica(NJ1株)のQS機構の特性および活性制御メカニズムを調べた。アシル化ホモセリンラクトン(AHL)合成遺伝子を大腸菌に組み込んで代理発現させた結果、4種類のAHLが検出され、これらのAHLが同株の亜硝酸酸化還元酵素をコードする遺伝子を制御していることが明らかになった。

polymer brushを有する機能界面を利用した生体高分子の三次元集積化

1996

Research Results Outline: まず、多孔性膜の界面から polymer brushがはえている構造を作成するために、中空糸状の多孔性膜(ポリエチレン製)に電子線を照射した後、エポ まず、多孔性膜の界面から polymer brushがはえている構造を作成するために、中空糸状の多孔性膜(ポリエチレン製)に電子線を照射した後、エポキシ基をもつモノマ-(グリシジルメタクリレート)を前駆体としてグラフト重合させた。その後、エポキシ... まず、多孔性膜の界面から polymer brushがはえている構造を作成するために、中空糸状の多孔性膜(ポリエチレン製)に電子線を照射した後、エポキシ基をもつモノマ-(グリシジルメタクリレート)を前駆体としてグラフト重合させた。その後、エポキシ基の一部をイオン交換基(ジエチルアミノ基)に、残りをアルコール性水酸基(エタノールアミノ基)へ変換した.この多孔性膜の膜間に圧力をかけて溶液を透過させ、牛血清アルブミン(BSA)を対流に乗せて polymer brushまで運び、きわめて短時間で生体高分子集合体を創製できることを確認した。 この膜に一定流量でBSA溶液を透過させたときの圧力損失は、BSAの吸着が進むにつれて増大する。楕円球のBSA分子がpolymer brushに最密充填的に吸着し、液の透過できる細孔径を減少させると仮定すると、BSA吸着後の膜の透過圧力はHagen-Poiseuille式によって推算できる。この理論式より推算された透過圧力と圧力センサーにより実測した透過圧力を比較した結果、両者はよく一致し、吸着容量から推測した多層吸着構造モデルの妥当性が、流体力学的側面からも裏付けられた。また、polymer brush中のイオン交換基密度の増加とともに、タンパク質吸着量が増大することも同時に示された。 次に、蛍光プローブ法によって、polymer brushのキャラクタリゼーションを行った。グラフト重合量や溶媒環境を変えて、蛍光スペクトルのピーク波長シフトを測定することによって、polymer brushのコンフォメーションを反映する蛍光プローブのまわりの極性の情報が得られた。この結果から、水中では、イオン交換基密度の増加とともに、polymer brushが電荷同士の反発によって表面法線方向に伸長し、結果的にタンパク質をより多く抱き込む形態を作ることが確認できた。

排水処理に有効な生物膜を対象とした微生物生態系に関する研究

1997

Research Results Outline:硝化細菌は廃水処理プロセスにおいて大きな役割を果たすが、生物膜内でどのように共存し、また諸環境因子によりそれらの共存形態がどのように変遷するかはほとん硝化細菌は廃水処理プロセスにおいて大きな役割を果たすが、生物膜内でどのように共存し、また諸環境因子によりそれらの共存形態がどのように変遷するかはほとんど明らかになっていない。本研究では、水温、pH等の変動による水質の変化と好気槽生物膜内における硝化...硝化細菌は廃水処理プロセスにおいて大きな役割を果たすが、生物膜内でどのように共存し、また諸環境因子によりそれらの共存形態がどのように変遷するかはほとんど明らかになっていない。本研究では、水温、pH等の変動による水質の変化と好気槽生物膜内における硝化細菌の個体数の関係を追跡すると同時に、生物膜内での生態系(分布特性)を明らかにすることを目的として検討を行った。 アンモニア硝化能に及ぼす水温の影響に関して調べた結果、水温は硝化の初期、つまり硝化細菌が生物膜を形成する段階において、硝化細菌の増殖至適温度である30℃付近における制御が重要となるが、一定の生物膜が形成されると、水温に対して耐性ができ、硝化能を保持する上では水温の影響は小さくなることがわかった。一方、10℃では生物膜が形成されなかったため硝化活性は極めて低かった。 硝化能に及ぼすpHの影響に関しては、20℃でpHを7.4に制御した系が、8.5に制御した系や制御しなかった系に比べ、連続実験期間を通して硝化細菌の高い増殖能および硝化活性を示した。また、pHをコントロールしなかった場合のpHは概ね5.0付近であった。これより無機廃水の硝化における至適pHは7.0~8.0付近であり、安定した硝化活性を維持する上で、pHの至適範囲における連続制御の重要性が示唆された。 接触材に付着した生物膜中の硝化細菌数と温度やpHとの相関性を明らかにするために、抗Nitrosomonas europaeaモノクローナル抗体を用いた迅速定量化手法により担体単位面積あたりのN. europaeaの個体数を測定した。20℃でpHコントロールしなかった系では、7.4にコントロールした系に比べN. europaeaの個体数はほぼ10分の1であった。以上よりpHを7.4にコントロールした場合、生物膜内の硝化細菌を高密度で保持できることが確認された。発表論文:1998年1月、野田尚宏、平田彰、常田聡、稲森悠平、第5回生物利用新技術研究シンポジウム論文集、 226-230生物膜の硝化菌バイオマス量と硝化能との関係

高硝酸・高塩濃度産業廃液からの生物学的窒素除去に関する研究

1998

Research Results Outline: ロンドン条約の改正・施行に伴い、1996年1月1日より酸・アルカリ廃液の海洋投棄処分が禁止となった。また、湖沼・河川の富栄養化等の防止上、下水道放 ロンドン条約の改正・施行に伴い、1996年1月1日より酸・アルカリ廃液の海洋投棄処分が禁止となった。また、湖沼・河川の富栄養化等の防止上、下水道放流に関しても窒素の排水規制が厳しくなる方向にある。このような状況の中、LSI・電子チップ等の廃棄物... ロンドン条約の改正・施行に伴い、1996年1月1日より酸・アルカリ廃液の海洋投棄処分が禁止となった。また、湖沼・河川の富栄養化等の防止上、下水道放流に関しても窒素の排水規制が厳しくなる方向にある。このような状況の中、LSI・電子チップ等の廃棄物より金・銀・パラジウム・白金といった貴金属を王水・アンモニア等により回収する際に排出される廃液(以後、製錬廃液と呼ぶ)は、硝酸・アンモニアといった酸・アルカリを大量に含んでおり、適切な処理法の開発が求められている。しかし、廃液には数%から十数%と非常に高濃度の無機塩類が含まれているため、通常生物処理が困難であると考えられている。 以上の背景を踏まえ本研究では、すでに獲得した高塩濃度下でも活性を示す微生物(硝化菌・脱窒菌)を用いて、嫌気好気生物膜循環法による実験装置において、製錬実廃液を窒素の下水道放流規制値(T-N 240g/m3)以下まで処理するための操作条件を模索した。また、嫌気槽好気槽の最適な容積比、循環比等の計算を行った。 循環比4という条件下で連続生物処理実験を行った結果、徐々に滞留時間を短縮しながら微生物を馴養することによって、2~5倍希釈の製錬実廃液を下水道放流規制値まで処理することができた。また、滞留時間はスタート時の1/3以下まで短縮することができた。次に、循環比4の連続処理実験の結果に基づいて、規制値を満足し、且つ装置全体の滞留時間が最小となる嫌気槽好気槽の容積比を求めた。嫌気槽での脱窒、好気槽での硝化がともに1次反応近似可能であり、嫌気槽では脱窒のみ、好気槽では硝化のみしか行われないと仮定した場合、トータルの滞留時間を示す式を容積比の関数で表すことができた。そして、最小の滞留時間を与える容積比は嫌気槽 : 好気槽 = 1 : 1であることがわかった。さらに、循環比の値を変化させ、規制値を満足するような滞留時間を求めた結果、トータルの滞留時間が最小となる循環比は9であることがわかった。

有用微生物の高機能化・高密度固定化技術を利用した写真廃液の生物処理システムの開発

2000

Research Results Outline: ロンドン条約附属書Iの改正に伴い、1995年末をもって写真廃液を含む産業廃棄物の海洋投棄処分が禁止された。写真廃液はハイドロキノンやカプラー、界面活 ロンドン条約附属書Iの改正に伴い、1995年末をもって写真廃液を含む産業廃棄物の海洋投棄処分が禁止された。写真廃液はハイドロキノンやカプラー、界面活性剤、EDTAなどの難生分解性物質を含むほか、無機塩類や有機性窒素化合物なども高濃度に含まれており... ロンドン条約附属書Iの改正に伴い、1995年末をもって写真廃液を含む産業廃棄物の海洋投棄処分が禁止された。写真廃液はハイドロキノンやカプラー、界面活性剤、EDTAなどの難生分解性物質を含むほか、無機塩類や有機性窒素化合物なども高濃度に含まれており、難分解性の廃液である。我々は写真廃液には硫酸塩を高濃度に含有していることに着目し、硫酸塩還元細菌を利用した難生分解性有機物除去プロセスの構築を目的として検討を行った。本研究では、写真廃液に含まれるFe-EDTAにターゲットを絞り、Fe-EDTA分解微生物群の獲得や硫酸塩還元細菌の分解寄与の確認、Fe-EDTA分解微生物群による分解過程での中間生成物の有無の確認を行った。 Fe-EDTAを分解する硫酸塩還元細菌を探索するため、水田から汚泥を採取し、硫酸塩還元細菌選択培地に炭素源としてFe-EDTAが100mg-C/Lとなるように添加し、約6ヶ月間馴養を行った。その結果、Fe-EDTAの顕著な減少が見られ、約10日で濃度をゼロにまで低減することに成功した。ここで、Fe-EDTAの分解における硫酸塩還元細菌の寄与を確認するため、硫酸塩還元細菌の阻害剤およびメタン生成細菌の阻害剤をそれぞれ添加し、分解試験を行った。その結果、硫酸塩還元細菌の阻害剤であるモリブデン酸ナトリウムを2mM添加した場合のみFe-EDTAの減少が見られなかったことから、硫酸塩還元細菌によるFe-EDTA分解寄与が示唆された。さらに、Fe-EDTAを分解させる際、Yeast Extractが必要であること、および分解中間生成物を生成していることがそれぞれ明らかとなり、Fe-EDTAの分解に寄与する硫酸塩還元細菌は水素を電子供与体とする細菌である可能性が高いことが示唆された。

水環境浄化に寄与するバクテリアの環境適応機構の解明と促進化技術の開発

2002

Research Results Outline: 微生物を利用した廃水処理においては,微生物の機能をいかにして向上させるかは重要なテーマである。我々は,廃水処理プロセスにおいて汎用的に行なわれている 微生物を利用した廃水処理においては,微生物の機能をいかにして向上させるかは重要なテーマである。我々は,廃水処理プロセスにおいて汎用的に行なわれている「馴養」と呼ばれる操作に着目した。馴養とは,微生物を一定期間同一条件下に置くことで,微生物をその環... 微生物を利用した廃水処理においては,微生物の機能をいかにして向上させるかは重要なテーマである。我々は,廃水処理プロセスにおいて汎用的に行なわれている「馴養」と呼ばれる操作に着目した。馴養とは,微生物を一定期間同一条件下に置くことで,微生物をその環境に馴らし,機能向上を行う操作のことである。本研究では,廃水処理プロセス中から耐塩性脱窒細菌を単離,培養し,高塩濃度環境への馴化を試み,さらにそのメカニズムを明らかにすることをめざした。 まず,解析モデルとなる耐塩性脱窒細菌の単離・培養を試みた。無機塩類および硝酸を高濃度に含む製錬廃水を処理しているリアクターの嫌気層から種汚泥を採取し,約8%のNaClを含む寒天プレート上にコロニーを形成させた。得られたコロニーを採取し,液体培地中で静置培養した。液体培地中で気体の発生が見られたものについては,集積培養を行って単離した。その結果,耐塩性脱窒細菌を単離することに成功した。16SrDNAによる系統解析から,この単離菌は,Bacillus halodenitrificans の近縁種であることがわかった。 単離した脱窒細菌を用いて,高塩濃度条件下(NaCl濃度約16%)で長期馴養を行った。その後,高塩濃度条件下で長期馴養を行った系と行わなかった系とで脱窒活性の比較をした。その結果,高塩濃度条件下で長期馴養を行った場合のみ高塩濃度条件下で脱窒活性の向上が見られた。 最後に,二次元電気泳動により馴養株と非馴養株の発現タンパク質について調べたところ,馴養前後で異なるタンパク質発現が確認された。馴養によってタンパク質発現レベルでの変化が起こり得ることが示唆され,馴養が微生物群集構造の変化以外にも影響を及ぼすことがわかった。

バイオフィルム形成における微生物生態の数学的シミュレーションモデルの構築

2004

Research Results Outline:自然環境中の微生物は,単独に浮遊して存在することはきわめて稀であり,その多くは微生物自身が生成する細胞外ポリマー(EPS)などにより固体表面に付着し,自然環境中の微生物は,単独に浮遊して存在することはきわめて稀であり,その多くは微生物自身が生成する細胞外ポリマー(EPS)などにより固体表面に付着し,複雑な微生物コミュニティー,バイオフィルムを形成して存在している.このバイオフィルムは環境浄化プロ...自然環境中の微生物は,単独に浮遊して存在することはきわめて稀であり,その多くは微生物自身が生成する細胞外ポリマー(EPS)などにより固体表面に付着し,複雑な微生物コミュニティー,バイオフィルムを形成して存在している.このバイオフィルムは環境浄化プロセスにおいて大きな効用をもたらす反面,金属腐食やパイプのつまり,浄水の汚染などの原因にもなる.そこで,バイオフィルムの生態構造(種類,数,場所,活性など)やメカニズムを解明することが重要となってくる.解明された情報を基に,上記の現象を再現,予測することが可能となれば,環境浄化プロセスの設計・制御を行う際に非常に有用である.このために有効なのが数学的シミュレーションである.本研究では,2次元のCellular Automaton(CA)-hybrid modelを用いてバイオフィルムの多菌種多基質シミュレーションモデルの構築を行った。対象とする基質はC,NH4,NO2,NO3,O2,菌種は従属栄養細菌(HB),アンモニア酸化細菌(AOB),亜硝酸酸化細菌(NOB),不活性バイオマスである.また,酸素供給方向の異なる2つのバイフィルムを対象とし、ポピュレーション変化をシミュレートした.1系は,O2が他の基質と同じ方向,つまりバルク側から供給されるTop-down aeration型であり,もう1系は,O2が他の基質とは逆の方向,つまり担体側から供給されるMembrane aeration型である.その結果,4菌種(HB,AOB,NOB,Dead Biomass)において,Top-down型に比べMembrane型の方が,AOBの割合が大きくなったが,極端に異なる結果にはいたらなかった.一方、3菌種(AOB,NOB,Dead Biomass)において,Top-down型ではAOBが生菌の大半を占めていたが,Membrane型では時間経過によってAOBとNOBの割合が変化するという解析結果を得た.

表面科学技術を応用した次世代型金属回収式バイオリアクターの開発

2004

Research Results Outline:多くの重金属イオンは生体への毒性が高く,また食物連鎖による生物濃縮が起こるため,金属成分を含む廃水を排出する事業所ではこれを適正に処理しなければならな多くの重金属イオンは生体への毒性が高く,また食物連鎖による生物濃縮が起こるため,金属成分を含む廃水を排出する事業所ではこれを適正に処理しなければならない.特にカドミウム(Cd)については,1955年富山県神通川流域で起こったイタイイタイ病の原因であ...多くの重金属イオンは生体への毒性が高く,また食物連鎖による生物濃縮が起こるため,金属成分を含む廃水を排出する事業所ではこれを適正に処理しなければならない.特にカドミウム(Cd)については,1955年富山県神通川流域で起こったイタイイタイ病の原因であることから,回収技術の一層の高度化が求められ,排水基準値は0.1mg/L以下という極めて低い値が設定されている.そこで,我々は廃水中の重金属除去技術として硫酸塩還元細菌(SRB)を用いた生物学的金属硫化法を提案した.本研究ではFe-Niスラグウール(FS)を付着担体とした硫酸還元バイオリアクターによる希薄Cd含有廃水処理を試みた.100日間にわたる連続処理実験を行い,安定性を確認するとともに,滞留時間(HRT)を徐々に短縮し,この装置の限界および処理が悪化した際の挙動を調べた.その結果,運転開始直後は処理が不安定であったが,以降100日を越える期間安定したCd除去が行われた.リアクター出口におけるCd濃度は0~0.04mg/L(Cd除去率として99%以上)であり,排水基準値である0.1mg/Lを大きく下回る水質が得られた.また,数学モデルを構築して,連続実験の結果に対するシミュレーションを行い,リアクター内における溶存成分の挙動を予測した.その結果,Cd2+,全有機炭素(TOC),SO42-いずれの成分についても高い相同性をもつことが示され,硫酸還元バイオリアクター内の生物化学反応を適切に表現する数学モデルを構築できたことが確認された.さらに,本廃水処理装置を用いて鉱山廃水を模擬した重金属複合成分系(Cu,Cd,Zn,Ni,Mn)で80日間の連続処理実験を行った結果,Cu,Cd,Zn,Niについては回収率ほぼ100%を達成することができ,各重金属元素の排水基準値以下を達成する良好な処理が行うことができた.

嫌気性アンモニア酸化細菌を活用した低コスト・省スペース型排水処理プロセスの開発

2006

Research Results Outline: 近年発見された嫌気性アンモニア酸化反応を排水処理技術に応用することができれば,単一の反応槽で窒素除去を行うことができ,従来の硝化・脱窒法に比べ,酸素 近年発見された嫌気性アンモニア酸化反応を排水処理技術に応用することができれば,単一の反応槽で窒素除去を行うことができ,従来の硝化・脱窒法に比べ,酸素供給量,水素供与体の補填量の削減,余剰汚泥の発生量の抑制が可能となることから,大幅なコスト削減が実... 近年発見された嫌気性アンモニア酸化反応を排水処理技術に応用することができれば,単一の反応槽で窒素除去を行うことができ,従来の硝化・脱窒法に比べ,酸素供給量,水素供与体の補填量の削減,余剰汚泥の発生量の抑制が可能となることから,大幅なコスト削減が実現できる。本研究では,分子生物学的手法を活用し,嫌気性アンモニア酸化細菌の生態学を明らかにするとともに,嫌気性アンモニア酸化細菌の大量培養技術の開発,固定化技術の開発,さらにこの菌体を用いた新規排水処理技術の確立を行うことを目的とした。 まず,これまで培ってきた硝化細菌や脱窒細菌の培養技術を応用し,嫌気性アンモニア酸化細菌の集積技術および連続培養技術に取り組んだ。上向流型リアクターにポリエステル繊維不織布を充填し,アンモニアと亜硝酸をそれぞれ35mg/L含む培養液を供給した結果,120日程度でアンモニアと亜硝酸が同時に減少する嫌気性アンモニア酸化反応が確認された。実験では3種類の種汚泥(下水処理施設から採取した余剰汚泥および消化汚泥,養豚廃水処理施設から採取した硝化汚泥)を用いたが,いずれの場合もほぼ同様の結果を得ることができた。つぎに,滞留時間を徐々に減少させることで嫌気性アンモニア酸化細菌の増殖速度を高め,最終的に脱窒速度9.8kg-N/m3/dを得た。この実験の際に得られた脱窒速度の上昇カーブから,嫌気性アンモニア酸化細菌の増殖速度が,既報告値より格段に速いことが示唆された。そこで,嫌気性アンモニア酸化細菌の菌数変化をFISH法により定量的に評価した結果,倍加時間が1.8日であり,従来報告されている倍加時間11日に比べて増殖速度が5倍以上であることを明らかにした。つぎに,上記の方法で集積培養した嫌気性アンモニア酸化細菌群をポリエチレングリコール(PEG)ゲルに包括固定化することに成功し,活性の低下がほとんどないことを確認した。最後に,このゲルをリアクター内に投入し,模擬排水の処理試験を行った結果,低水温下や有機物共存下においても長期にわたり安定した窒素除去特性を得られることを確認し,本技術の実用性の高さを証明した。

微生物凝集および微生物生態の理解による高速アンモニア処理システム構築

2009Collaborator:青井 議輝, 大坂 利文

Research Results Outline:これまでに我々は,上向流好気性流動床リアクターを用いて硝化細菌グラニュールの形成に成功している。硝化細菌グラニュールは硝化細菌を高密度に保持し,沈降性これまでに我々は,上向流好気性流動床リアクターを用いて硝化細菌グラニュールの形成に成功している。硝化細菌グラニュールは硝化細菌を高密度に保持し,沈降性が極めて高いため,排水中の窒素除去の高効率化に有効である。しかしながら,硝化細菌グラニュールの形成...これまでに我々は,上向流好気性流動床リアクターを用いて硝化細菌グラニュールの形成に成功している。硝化細菌グラニュールは硝化細菌を高密度に保持し,沈降性が極めて高いため,排水中の窒素除去の高効率化に有効である。しかしながら,硝化細菌グラニュールの形成メカニズムは未だ不明確な点が多く,実用化への大きな障害となっている。本研究では,分子生物学的手法を用いて硝化細菌グラニュールの微生物生態構造を解析し,どのような細菌種がグラニュール形成に寄与しているかを明らかにすることを目的とした。まず,クローニング法を用いて硝化細菌グラニュールに存在する細菌種の群集構造解析を行った。その結果,16S rRNA遺伝子解析から,完全無機条件で馴養された硝化細菌グラニュールには,硝化細菌以外にも有機物を消費する多様な従属栄養細菌が存在し,複雑な生態系が構築されていることが明らかになった。また,アンモニア酸化酵素amoAをコードする機能遺伝子解析から,グラニュール形成に伴いNitrosomonas mobilisに近縁なクローンとアミノ酸配列が高い相同性を示すクローンが得られたため,初期形成中のアンモニア酸化細菌(AOB)はN. mobilis系統に属することが示唆された。さらに,fluorescence in situ hybridization(FISH)法を用いて各細菌種の存在割合,空間分布解析を行った結果,N. mobilis系統に属するAOBは,初期形成グラニュールにおいて優占化していることが明らかになった。以上の結果より,グラニュール形成においてN. mobilis系統に属するAOBが選択的に凝集・粒状化し,初期のグラニュール形成に重要な役割を果たしていることが示唆された。

次世代癌研究ツールとしてのイン・シリコ癌シミュレーターの開発

2009Collaborator:加川 友己

Research Results Outline: 本研究は、システム工学的アプローチで医学研究を推進する「システム医工学」という新しい概念を癌研究に適用し、現在進行的に入手可能な、癌に対する実験・観 本研究は、システム工学的アプローチで医学研究を推進する「システム医工学」という新しい概念を癌研究に適用し、現在進行的に入手可能な、癌に対する実験・観察事実をコンピュータ上に集約・再構成することで、発癌プロセスを統一的に理解するための新規方法論を確... 本研究は、システム工学的アプローチで医学研究を推進する「システム医工学」という新しい概念を癌研究に適用し、現在進行的に入手可能な、癌に対する実験・観察事実をコンピュータ上に集約・再構成することで、発癌プロセスを統一的に理解するための新規方法論を確立・提案することを目的としている。具体的には大腸癌に焦点を絞り、(1)発癌の場となる大腸陰窩(いんか)のシミュレーションモデルの構築、およびそのモデルをベースとした(2)大腸癌における多段階発癌モデルの数理モデル化によって、発癌プロセスを統一的に理解する土台の構築を試みたが、時間的制約により(1)を中心とした研究を実施した。 まず陰窩における細胞の増殖・分化の時空間構造を可視化するシミュレーションモデルを構築した。これは陰窩を底のある円筒で表現し、その円筒の展開図内で個々の細胞が独立に増殖・分化していくモデルであり、個体ベースモデルのアルゴリズムを参考に構築した。次にヒト大腸陰窩において得られている pLIデータを再現するようなモデルパラメータセットの探索を行った。ここでpLI(positional Labeling Index)とは、ある短い時間間隔においてDNAを合成した細胞のみをBrdU等によってラベル化し、ラベル化された細胞の割合を陰窩の底からの位置の関数として求めたものであり、分裂細胞の空間配置を調べる手法として多用されている。本研究ではBrdU取り込み過程の計算機シミュレーションを行い、その結果得られる画像データ(ラベル化細胞の空間配置パターン)を多数取得し、それらの画像解析・統計処理を行うことでpLIデータを取得した。モデルパラメータ空間の各点において、これらシミュレーションを繰り返すことで、実験データに最も良くフィットするパラメータセットを見出すことに成功した。 また、FAP(家族性大腸腺腫症;がん抑制遺伝子APCに変異がある)患者の陰窩で得られるpLIデータへのフィッティングも試みた。その結果、FAP患者の陰窩では幹細胞数、分化するまでの世代数、幹細胞周期が正常陰窩に比べ増大していることが、本モデルから予測された。

Lecture Course

Course TitleSchoolYearTerm
Introduction to Bioscience A DenshibutsuriSchool of Fundamental Science and Engineering2019spring semester
Introduction to Bioscience A Kenchiku, keiei, shakou, shigenSchool of Creative Science and Engineering2019spring semester
Introduction to Bioscience B SeiiSchool of Advanced Science and Engineering2019spring semester
Science and Engineering Laboratory 1A ISchool of Fundamental Science and Engineering2019spring semester
Science and Engineering Laboratory 1A ISchool of Creative Science and Engineering2019spring semester
Science and Engineering Laboratory 1A ISchool of Advanced Science and Engineering2019spring semester
Science and Engineering Laboratory 1B IISchool of Fundamental Science and Engineering2019fall semester
Science and Engineering Laboratory 1B IISchool of Creative Science and Engineering2019fall semester
Science and Engineering Laboratory 1B IISchool of Advanced Science and Engineering2019fall semester
Introduction to Bioscience A SougoukikaiSchool of Creative Science and Engineering2019spring semester
Introduction to Bioscience A Kagaku, OukaSchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Seminar ISchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Seminar I [S Grade]School of Advanced Science and Engineering2019spring semester
MicrobiologySchool of Advanced Science and Engineering2019fall semester
Microbiology [S Grade]School of Advanced Science and Engineering2019fall semester
Life Science and Medical Bioscience LaboratorySchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory ISchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory I [S Grade]School of Advanced Science and Engineering2019spring semester
Analytical Chemistry BSchool of Advanced Science and Engineering2019fall semester
Analytical Chemistry B [S Grade]School of Advanced Science and Engineering2019fall semester
Applied Mathematics ASchool of Advanced Science and Engineering2019spring quarter
Applied Mathematics A [S Grade]School of Advanced Science and Engineering2019spring quarter
Life Science and Medical Bio-science Seminar IISchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bio-science Seminar II [S Grade]School of Advanced Science and Engineering2019fall semester
Graduation ResearchSchool of Advanced Science and Engineering2019full year
Graduation Research [S Grade]School of Advanced Science and Engineering2019full year
Advanced Molecular MicrobiologySchool of Advanced Science and Engineering2019spring semester
BioengineeringSchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bio-science Laboratory IVSchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory VSchool of Advanced Science and Engineering2019fall semester
Life Science and Medical Bioscience Seminar IISchool of Advanced Science and Engineering2019spring semester
Bioscience Practicals ASchool of Advanced Science and Engineering2019fall semester
Bioscience Practicals BSchool of Advanced Science and Engineering2019spring semester
Graduation Thesis ASchool of Advanced Science and Engineering2019fall semester
Graduation Thesis BSchool of Advanced Science and Engineering2019spring semester
Master's Thesis (Department of Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2019full year
Research on Environmental BiotechnologyGraduate School of Advanced Science and Engineering2019full year
Research on Environmental BiotechnologyGraduate School of Advanced Science and Engineering2019full year
Advanced BioengineeringGraduate School of Advanced Science and Engineering2019fall semester
Advanced BioengineeringGraduate School of Advanced Science and Engineering2019fall semester
Advanced BioengineeringGraduate School of Advanced Science and Engineering2019fall semester
Seminar on Environmental Biotechnology AGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Environmental Biotechnology AGraduate School of Advanced Science and Engineering2019spring semester
Seminar on Environmental Biotechnology BGraduate School of Advanced Science and Engineering2019fall semester
Seminar on Environmental Biotechnology BGraduate School of Advanced Science and Engineering2019fall semester
Master's Thesis (Department of Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2019full year
Research on Environmental BiotechnologyGraduate School of Advanced Science and Engineering2019full year
Practical Training for Career BuildingGraduate School of Advanced Science and Engineering2019full year
Advanced Lifescience : Bio-Solid State PhysicsGraduate School of Advanced Science and Engineering2019fall quarter
Introduction of Life Science 01Global Education Center2019spring semester
Introduction of Life Science 02Global Education Center2019spring semester