Name

HOSOKAWA, Masahito

Official Title

Junior Researcher(Assistant Professor)

Affiliation

(Waseda Research Institute for Science and Engineering)

Contact Information

URL

Grant-in-aids for Scientific Researcher Number
60722981

Sub-affiliation

Affiliated Institutes

規範科学総合研究所

研究所員 2018-2019

規範科学総合研究所

研究所員 2019-

革新的生物資源利用研究所

研究所員 2019-2019

Paper

In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy

Miyaoka, Rimi;Hosokawa, Masahito;Ando, Masahiro;Mori, Tetsushi;Hamaguchi, Hiro-o;Takeyama, Haruko

MARINE DRUGS 12(5) p.2827 - 28392014-2014

DOIWoS

Detail

ISSN:1660-3397

In Vivo Live Cell Imaging for the Quantitative Monitoring of Lipids by Using Raman Microspectroscopy

Hosokawa, Masahito;Ando, Masahiro;Mukai, Shoichiro;Osada, Kyoko;Yoshino, Tomoko;Hamaguchi, Hiro-o;Tanaka, Tsuyoshi

ANALYTICAL CHEMISTRY 86(16) p.8224 - 82302014-2014

DOIWoS

Detail

ISSN:0003-2700

Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes

Hosokawa, Masahito;Hoshino, Yuri;Nishikawa, Yohei;Hirose, Tomotada;Yoon, Dong Hyun;Mori, Tetsushi;Sekiguchi, Tetsushi;Shoji, Shuichi;Takeyama, Haruko

BIOSENSORS & BIOELECTRONICS 67p.379 - 3852015-2015

DOIWoS

Detail

ISSN:0956-5663

In situ detection of antibiotic amphotericin B produced in Streptomyces nodosus using Raman microspectroscopy.

Miyaoka Rimi;Hosokawa Masahito;Ando Masahiro;Mori Tetsushi;Hamaguchi Hiro-O;Takeyama Haruko

In situ detection of antibiotic amphotericin B produced in Streptomyces nodosus using Raman microspectroscopy. 12(5) 2014-2014

DOI

Detail

ISSN:1660-3397

Outline::The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes-Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling.

Patent

Reference Number:1585

食肉の加熱処理状況の判定方法、食肉の加熱処理状況の判定装置及びプログラム(日本)

濱口 宏夫, 安藤 正浩, 竹山 春子, 細川 正人

2014-231539、2016- 95230

Research Grants & Projects

Grant-in-aids for Scientific Research Adoption Situation

Research Classification:

Development of platform for ultra high-throughput screening of novel bioactive compound producers

2017/-0-2022/-0

Allocation Class:¥205010000

Research Classification:

Development of single-cell whole genome amplification method for uncultivable bacteria using droplet microfluidics

2014/-0-2016/-0

Allocation Class:¥3900000

Research Classification:

Development of technique for digital counting of DNA copy number at the single-cell level

2012/-0-2015/-0

Allocation Class:¥18980000

Research Classification:

Advanced Micro Fluidic Engineering and Its Applications for High Sensitive Quantitative Measurements of Biomolecules

2011/-0-2016/-0

Allocation Class:¥215930000

Lecture Course

Course TitleSchoolYearTerm
Life Science and Medical Bioscience LaboratorySchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory ISchool of Advanced Science and Engineering2019spring semester
Life Science and Medical Bio-science Laboratory I [S Grade]School of Advanced Science and Engineering2019spring semester
Marine Life Resources and EngineeringSchool of Advanced Science and Engineering2019fall quarter
Current Topics in BiosciencesSchool of Advanced Science and Engineering2019fall semester
Current Topics in Biosciences [S Grade]School of Advanced Science and Engineering2019fall semester
Molecular Cell Biology ASchool of Advanced Science and Engineering2019fall semester
Molecular Cell Biology ASchool of Advanced Science and Engineering2019fall semester
Molecular Cell Biology A [S Grade]School of Advanced Science and Engineering2019fall semester
Environmental BioresourcesGraduate School of Advanced Science and Engineering2019fall quarter
Environmental BioresourcesGraduate School of Advanced Science and Engineering2019fall quarter
Advanced Biomolecular Science and Engineering (Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2019fall quarter
Advanced Biomolecular Science and Engineering (Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2019fall quarter