Name

TAKEYAMA, Haruko

Official Title

Professor

Affiliation

(School of Advanced Science and Engineering)

Contact Information

Mail Address

Mail Address
haruko-takeyama@waseda.jp

URL

Web Page URL

http://www.f.waseda.jp/haruko-takeyama/index.html

Grant-in-aids for Scientific Researcher Number
60262234

Sub-affiliation

Sub-affiliation

Faculty of Science and Engineering(Graduate School of Advanced Science and Engineering)

Research Council (Research Organization)/Affiliated organization(Global Education Center)

Affiliated Institutes

ライフサポートイノベーション研究所

研究所員 2014-2014

欧州バイオメディカルサイエンス研究所

研究所員 2011-2012

規範科学総合研究所

プロジェクト研究所所長 2014-2014

欧州バイオメディカルサイエンス研究所

研究所員 2009-2011

実践的ナノ化学G

研究所員 2007-2011

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2012-2014

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2015-2016

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2016-2017

東日本大震災復興研究拠点・先端環境医工科学研究所

研究所員 2011-2013

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2017-2018

ライフサポートイノベーション研究所

研究所員 2015-2019

規範科学総合研究所

研究所員 2015-2019

持続型食・農・バイオ研究所

研究所員 2014-2018

革新的生物資源利用研究所

プロジェクト研究所所長 2019-2019

革新的生物資源利用研究所

研究所員 2019-2019

リンアトラス研究所

研究所員 2015-

規範科学総合研究所

プロジェクト研究所所長 2015-

早稲田バイオサイエンスシンガポール研究所

研究所員 2017-

理工学術院総合研究所(理工学研究所)

兼任研究員 2018-

ライフサポートイノベーション研究所

研究所員 2019-

規範科学総合研究所

研究所員 2019-

Educational background・Degree

Degree

Doctor of Engineering Coursework Tokyo University of Agriculture and Technology

Academic Society Joined

Japanese Society of Bacteriology

The Chemical Society of Japan

The Electrochemical Society of Japan

The Society of Biotechnology, Japan

International Society for Microbial Ecology

American Society for Microbiology

Japanese Society for Marine Biotechnology

The Molecular Biology Society of Japan

Others Basic Information

・ The 1st International Workshop on Approaches to Single-Cell Analysis, Co-organizer (Sweden 2006/6/21 - 6/22) ・The 2nd International Workshop on Approaches to Single-Cell Analysis, Co-organizer (Japan 2007/9/6 - 9/7)

Research Field

Keywords

Marine biotechnology,Genome engineering,Single cell analysis

Paper

Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking

H. Tsuyuzaki, M. Hosokawa, K. Arikawa, T. Yoda, N. Okada, H. Takeyama, M. Sato

Nature communications Peer Review Yes 11p.1265 - 12652020/03-

Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota

R. Chijiiwa, M. Hosokawa, M. Kogawa, Y. Nishikawa, K. Ide, C. Sakanashi, H. Takeyama

Microbiome Peer Review Yes 8p.1 - 12020/03-

Rapid inspection method for investigating the heat processing conditions employed for chicken meat using Raman spectroscopy

R. Miyaoka, M. Ando, R. Harada, H. Osaka, AZ Samuel, M Hosokawa, H. Takeyama

Journal of Bioscience and Bioengineering Peer Review Yes 2020/02-

Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAP-like vesicles via delocalization of NDP52

M. Ogawa, N. Takada, M. Tomokiyo, B. Chang, M. Yoshida, S. Kakuta, I. Tanida, J-L. Guan, H. Takeyama, M. Ohnishi

Communications Biology Peer Review Yes 3p.1 - 152020/01-

Exposure of an occluded hemagglutinin epitope drives selection of a class of cross-protective influenza antibodies

Y. Adachi, K. Tonouchi, A. Nithichanon, M. Kuraoka, A. Watanabe, R. Shinnakasu, H. Asanuma, A. Ainai, Y. Ohmi, T. Yamamoto, K. Ishii, H. Hasegawa, H. Takeyama, G. Lertmemongkolchai, T. Kurosaki, M. Ato, G. Kelsoe, Y. Takahashi

Nature Communications Peer Review Yes 10p.1 - 132019/08-

Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice

T. Matsumura, T. Ikebe, K. Arikawa, M. Hosokawa, M. Aiko, A. Iguchi, I. Togashi, S. Kai, S. Ohara, N. Ohara, M. Ohnishi, H. Watanabe, K. Kobayashi, H. Takeyama, S. Yamasaki, Y. Takahashi, M. Ato

Cell reports Peer Review Yes 27p.561 - 5712019/04-

Enrichment of bacteria and alginate lyase genes potentially involved in brown alga degradation in the gut of marine gastropods

M. Ito, K. Watanabe, T. Maruyama, T. Mori, K. Niwa, S. Chow, H.Takeyama

Scientific reports Peer Review Yes 9p.1 - 12019/02-

An estrogen antagonist, cyclofenil, has anti-dengue-virus activity

D. Tohma, S. Tajima, F. Kato, H. Sato, M. Kakisaka, T. Hishiki, M. Kataoka, H. Takeyama, CK. Lim, Y. Aida, M. Saijo

Archives of Virology Peer Review Yes 164p.225 - 2342019/01-

IgA tetramerization improves target breadth but not peak potency of functionality of anti-influenza virus broadly neutralizing antibody

S. Saito, K. Sano, T. Suzuki, A. Ainai, Y. Taga, T. Ueno, K. Tabata, K. Saito, Y. Wada, Y. Ohara, H. Takeyama, T. Odagiri, T. Kageyama, K. Ogawa-Goto, P. Multihartina, V. Setiawaty, KNA. Pangesti, H. Hasegawa

PLoS pathogens Peer Review Yes 15p.e1007247 - e10072472019/01-

Effects of short‐term endurance exercise on gut microbiota in elderly men

H. Taniguchi, K. Tanisawa, X. Sun, T. Kubo, Y. Hoshino, M. Hosokawa, H. Takeyama, M. Higuchi

Physiological reports Peer Review Yes 6p.e13935 - e139352018/12-

Acyclovir Sensitivity and Neurovirulence of Herpes Simplex Virus Type 1 with Amino Acid Substitutions in the Viral Thymidine Kinase Gene

T. Inagaki, M. Satoh, H. Fujii, S. Yamada, M. Shibamura, T. Yoshikawa, S. Harada, H. Takeyama, M. Saijo

Japanese Journal of Infectious Diseases Peer Review Yes 5p.343 - 3492018/09-

Molecular mechanisms of Streptococcus pneumoniae‐targeted autophagy via pneumolysin, Golgi‐resident Rab41, and Nedd4‐1 mediated K63‐linked ubiquitination

M. Ogawa, R. Matsuda, N. Takada, M. Tomokiyo, S. Yamamoto, S. Shizukusihi, T. Yamaji, Y. Yoshikawa, M. Yoshida, I. Tanida, M. Koike, M. Murai, H. Morita, H. Takeyama, A. Ryo, J. Guan, M. Yamamoto, J. Inoue, T. Yanagawa, M. Fukuda, H. Kawabw, M. Ohnishi

Cell Microbioloy Peer Review Yes 20p.e12846 - e128462018/08-

Introduction of Human Flt3-L and GM-CSF into Humanized Mice Enhances the Reconstitution and Maturation of Myeloid Dendritic Cells and the Development of Foxp3+CD4+ T Cells 

R. Iwabuchi, S. Ikeno, M. Kobayashi-Ishihara, H. Takeyama, M. Ato, Y. T. Yokota, K. Terahara

Frontiers Immunology Peer Review Yes 28p.1042 - 10422018/05-

A Novel System for Constructing a Recombinant Highly-Attenuated Vaccinia Virus Strain (LC16m8) Expressing Foreign Genes and Its Application for the Generation of LC16m8-Based Vaccines against Herpes Simplex Virus 2

N. Omura, T. Yoshikawa, H. Fujii, M. Shibamura, T. Inagaki, H. Kato, K. Egawa, S. Harada, S. Yamada, H. Takeyama, M. Saijo

Japanese Journal of Infectious Diseases Peer Review Yes 71p.229 - 2332018/03-

Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified gen

M. Kogawa, M. Hosokawa, Y. Nishikawa, K. Mori, H. Takeyama

Scientific Reports Peer Review Yes 8p.2059 - 20592018/02-

Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts

T. Mori, JKB. Cahn, MC. Wilson, RA. Meoded, V. Wiebach, AFC. Martinez, EJN. Helfrich, A. Albersmeier, D. Wibberg, S. Dätwyler, R. Keren, A. Lavy, C. Rückert, M. Ilan, J. Kalinowski, S. Matsunaga, H. Takeyama, J. Piel

PNAS Peer Review Yes 115p.1718 - 17232018/02-

Combinatory use of distinct single-cell RNA-seq analytical platforms reveals the heterogeneous transcriptome response

Y. Kashima, A. Suzuki, Y. Liu, M. Hosokawa, H. Matsunaga, M. Shirai, K. Arikawa, S. Sugano, T. Kohno, H. Takeyama, K. Tsuchihara, Y. Suzuki

Scientific Reports Peer Review Yes 8p.3482 - 34822018/02-

Genome-wide association study of Alzheimer's disease endophenotypes at prediagnosis stages

J. Chung, X. Wang, T. Maruyama, Y. Ma, X. Zhang, J. Mez, R. Sherva, H. Takeyama; Alzheimer's Disease Neuroimaging Initiative., KL. Lunetta, LA. Farrer, GR. Jun

Alzheimer's & Dementia Peer Review Yes 13p.337 - 3382017/12-

A humanized mouse model identifies key amino acids for low immunogenicity of H7N9 vaccines

Wada, Yamato; Wada, Yamato; Nithichanon, Arnone; Nithichanon, Arnone; Nobusawa, Eri; Moise, Leonard; Moise, Leonard; Martin, William D.; Yamamoto, Norio; Yamamoto, Norio; Terahara, Kazutaka; Hagiwara, Haruhisa; Odagiri, Takato; Tashiro, Masato; Lertmemongkolchai, Ganjana; Takeyama, Haruko; Groot, Anne S.De; Groot, Anne S.De; Ato, Manabu; Takahashi, Yoshimasa

Scientific Reports 7(1) 2017/12-2017/12

DOIScopus

Detail

Outline:© 2017 The Auth or(s). Influenza vaccines of H7N9 subtype are consistently less immunogenic in humans than vaccines developed for other subtypes. Although prior immunoinformatic analysis identified T-cell epitopes in H7 hemagglutinin (HA) which potentially enhance regulatory T cell response due to conservation with the human genome, the links between the T-cell epitopes and low immunogenicity of H7 HA remains unknown due to the lack of animal models reproducing the response observed in humans. Here, we utilized a humanized mouse model to recapitulate the low immunogenicity of H7 HA. Our analysis demonstrated that modification of a single H7 epitope by changing 3 amino acids so that it is homologous with a known H3 immunogenic epitope sequence significantly improved the immunogenicity of the H7 HA in the humanized mouse model, leading to a greater than 4-fold increase in HA-binding IgG responses. Thus, we provide experimental evidence for the important contribution of this H7-specific T cell epitope in determining the immunogenicity of an influenza vaccine. Furthermore, this study delineates strategies that can be used for screening and selecting vaccine strains using immunoinformatics tools and a humanized mouse model.

Induction of lung CD8(+) T cell responses by consecutive inoculations of a poly(I:C) influenza vaccine

M. Moriyama, H. Takeyama, H. Hasegawa, T. Ichinohe

Vaccine Peer Review Yes 35p.6620 - 66262017/11-

Transcriptome analysis of immune response against Vibrio harveyi infection in orange-spotted grouper (Epinephelus coioides)

S. Maekawa, O. Byadgi, YC. Chen, T. Aoki, H. Takeyama, T. Yoshida, J. Hikima, M. Sakai, PC. Wang, SC. Chen

Fish Shellfish Immunology Peer Review Yes 70p.628 - 6372017/09-

Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics

Hosokawa M, Nishikawa Y, Kogawa M, Takeyama H.

Scientific Reports Peer Review Yes 7p.51992017/07-

Whole-Genome Sequence of Photobacterium damselae subsp. piscicida Strain 91-197, Isolated from Hybrid Stri

Y. Teru, J Hikima, T. Kono, M. Sakai, T. Takano, JP. Hawke JP, H. Takeyama, T. Aoki

Genome Announcements Peer Review Yes 5p.e00600-172017/07-

Analysis of environmental bacteria at single-cell level

Hosokawa, Masahito; Hosokawa, Masahito; Nishikawa, Yohei; Kogawa, Masato; Kogawa, Masato; Takeyama, Haruko; Takeyama, Haruko; Takeyama, Haruko

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems p.634 - 6372017/07-2017/07

DOIScopus

Detail

Outline:© 2017 IEEE. Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.

Site-specific gene expression analysis using an automated tissue micro-dissection punching system

T. Yoda, M. Hosokawa, K. Takahashi, C. Sakanashi, H.Takeyama, H. Kambara

Scientific Reports Peer Review Yes 7p.43252017/06-

The complete genome sequence of Photobacterium damselae subsp. piscicida strain OT-51443 isolated from yellowtail (Seriola quinqueradiata) in Japan

Genome Announcements

Genome Announcements Peer Review Yes 5p.e00404-172017/05-

Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA.

Yoda T, Tanabe M, Tsuji T, Yoda T, Ishino S, Shirai T, Ishino Y, Takeyama H, Nishida H

Scientific Reports. 7:445822017/03-

Association between sensitivity of viral thymidine kinase-associated acyclovir-resistant herpes simplex virus 1 and virulence.

Omura N, Fujii H, Yoshikawa T, Yamada S, Harada S, Inagaki T, Shibamura M, Takeyama H, Saijo M

Virology Journal. DOI 10.1186/s12985-017-0728-22017-

SAG-QC: Quality control of single amplified genome by subtracting non-target sequences based on sequence compositions.

Maruyama T, Mori T, Yamagishi K, Takeyama H

Vol. 18(1).(doi: 10.1186/s12859-017-1572-5.) 2017-

Antimicrobial peptides extend lifespan in Drosophila.

Loch G, Zinke I, Mori T, Carrera P, Schroer J, Takeyama H, Hoch M

2017-

On-site Direct Detection of Astaxanthin from Salmon Fillet Using Raman Spectroscopy

Hikima J, Ando M, Hamaguchi H, Sakai M, Maita M, Yazawa K, Takeyama H, Aoki T.

Marine Biotchnology 19p.157 - 1632017-

Bivalent vaccine platform based on Japanese encephalitis virus (JEV) elicits neutralizing antibodies against JEV and hepatitis C virus.

Saga R, Fujimoto A, Watanabe N, Matsuda M, Hasegawa M, Watashi K, Aizaki H, Nakamura N, Tajima S, Takasaki T, Konishi E, Kato T, Kohara M, Takeyama H, Wakita T, Suzuki R

Sientific report 6p.286882016/06-

Falsirhodobacter sp. alg1 Harbors Single Homologs of Endo and Exo-Type Alginate Lyases Efficient for Alginate Depolymerization.

Mori T, Takahashi M, Tanaka R, Miyake H, Shibata T, Chow S, Kuroda K, Ueda M, Takeyama H

PloS one 11p.e01555372016/05-

Balancing intestinal and systemic inflammation through cell typespecific expression of the aryl hydrocarbon receptor repressor.

Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, Korkowski M, Haarmann-Stemmann T, von Smolinski D, Schultze JL, Abel J, Esser C, Takeyama H, Weighardt H, Förster I

Sientific report 6p.260912016/05-

Balancing intestinal and systemic inflammation through cell typespecific expression of the aryl hydrocarbon receptor repressor.

Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, Korkowski M, Haarmann-Stemmann T, von Smolinski D, Schultze JL, Abel J, Esser C, Takeyama H, Weighardt H, Förster I

Sientific report 6p.260912016/05-

The RNA-and TRIM25-binding domains of influenza virus NS1 protein are essential for suppression of NLRP3 inflammasome-mediated 1L-1β secretion.

Moriyama M, Chen IY, Kawaguchi A, Koshiba T, Nagata K, Takeyama H, Hasegawa H, Ichinohe T

Journal of Virology. 90p.4105 - 41142016/03-

Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome

Mori T, Iwamoto K, Iwamoto K, Wakaoji S, Araie H, Kohara Y, Okamura Y, Okamura Y, Shiraiwa Y, Takeyama H

Gene 576(2) p.618 - 6252016/02-2016/02

DOIScopus

Detail

ISSN:03781119

Outline:© 2015 Elsevier B.V.. Metagenome research has brought much attention for the identification of important and novel genes of industrial and pharmaceutical value. Here, using a metagenome library constructed from bacteria associated with the marine sponge, Styllisa massa, a high-throughput screening technique using radioisotope was implemented to screen for cadmium (Cd) binding or accumulation genes. From a total of 3301 randomly selected clones, a clone 247-11C was identified as harboring an open reading frame (ORF) showing Cd accumulation characteristics. The ORF, termed as ORF5, was further analyzed by protein functional studies to reveal the presence of a protein, Cdae-1. Cdae-1, composed of a signal peptide and domain harboring an E(G/A)KCG pentapeptide motif, enhanced Cd accumulation when expressed in Escherichia coli. Although showing no direct binding to Cd in vitro, the presence of important amino acid residues related to Cd detoxification suggests that Cdae-1 may possess a different mechanism from known Cd binding proteins such as metallothioneins (MTs) and phytochelatins (PCs). In summary, using the advantage of bacterial metagenomes, our findings in this work suggest the first report on the identification of a unique protein involved in Cd accumulation from bacteria associated with a marine sponge.

The effect of mucoadhesive excipient on the nasal retention time of and the antibody responses induced by an intranasal influenza vaccine.

Saito S, Ainai A, Suzuki T, Harada N, Ami Y, Yuki Y, Takeyama H, Kiyono H, Tsukada H, Hasegawa H

Vaccine. 34p.1201 - 12072016/01-

Homeostatically Maintained Resting Naive CD4(+) T Cells Resist Latent HIV Reactivation

Tsunetsugu-Yokota, Yasuko;Kobayahi-Ishihara, Mie;Wada, Yamato;Terahara, Kazutaka;Takeyama, Haruko;Kawana-Tachikawa, Ai;Tokunaga, Kenzo;Yamagishi, Makoto;Martinez, Javier P.;Meyerhans, Andreas

FRONTIERS IN MICROBIOLOGY 72016-2016

DOIWoS

Detail

ISSN:1664-302X

Draft Genome Sequence of the Fish Pathogen Mycobacterium pseudoshottsii Strain JCM15466, a Species Closely Related to M. marinum.

Hikima J, Sakai M, Aoki T, Takeyama H, Hawke J, Mori K, Tashiro K, Kuhara S

Genome annmouncement 4p.e01630-152015/12-

Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp

Lai, Hung Chiao; Ng, Tze Hann; Ando, Masahiro; Lee, Chung Te; Chen, I. Tung; Chuang, Jie Cheng; Mavichak, Rapeepat; Chang, Sheng Hsiung; Yeh, Mi De; Chiang, Yi An; Takeyama, Haruko; Hamaguchi, Hiro o.; Lo, Chu Fang; Aoki, Takashi; Wang, Han Ching

Fish and Shellfish Immunology 47(2) p.1006 - 10142015/12-2015/12

PubMedDOIScopus

Detail

ISSN:10504648

Outline:© 2015 Elsevier LtdAcute hepatopancreatic necrosis disease (AHPND), also called early mortality syndrome (EMS), is a recently emergent shrimp bacterial disease that has resulted in substantial economic losses since 2009. AHPND is known to be caused by strains of Vibrio parahaemolyticus that contain a unique virulence plasmid, but the pathology of the disease is still unclear. In this study, we show that AHPND-causing strains of V. parahaemolyticus secrete the plasmid–encoded binary toxin PirABvp into the culture medium. We further determined that, after shrimp were challenged with AHPND-causing bacteria, the bacteria initially colonized the stomach, where they started to produce PirABvp toxin. At the same early time point (6 hpi), PirBvp toxin, but not PirAvp toxin, was detected in the hepatopancreas, and the characteristic histopathological signs of AHPND, including sloughing of the epithelial cells of the hepatopancreatic tubules, were also seen. Although some previous studies have found that both components of the binary PirABvp toxin are necessary to induce a toxic effect, our present results are consistent with other studies which have suggested that PirBvp alone may be sufficient to cause cellular damage. At later time points, the bacteria and PirAvp and PirBvp toxins were all detected in the hepatopancreas. We also show that Raman spectroscopy “Whole organism fingerprints” were unable to distinguish between AHPND-causing and non-AHPND causing strains. Lastly, by using minimum inhibitory concentrations, we found that both virulent and non-virulent V. parahaemolyticus strains were resistant to several antibiotics, suggesting that the use of antibiotics in shrimp culture should be more strictly regulated.

Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome.

Mori T, Iwamoto K, Wakaoji S, Araie H, Kohara Y, Okamura Y, Shiraiwa Y, Takeyama H.

Gene. 576p.618 - 6252015/10-

Monodisperse Picoliter Droplets for Low-Bias and Contamination-Free Reactions in Single-Cell Whole Genome Amplification

Nishikawa Y, Hosokawa M, Maruyama T, Yamagishi K, Mori T, Takeyama H

PloS one 10p.e01387332015/09-

Metabolic and evolutionary origin of actin-binding polyketides from diverse organisms.

Ueoka R, Uria AR, Reiter S, Mori T, Karbaum P, Peters EE, Helfrich EJN, Morinaka BI, Gugger M, Takeyama H, Matsunaga S, Piel J

Nature chemical biology 11p.705 - 7122015/08-

A simple method for nanobubble generation and stability of the bubbles.

Ohmori M, Haruta K, Kamimura S, Koike H, Uchida T, Takeyama H

Journal of Experimental Biology. 15p.41 - 442015/08-

Analysis of the bacterial xylose isomerase gene diversity with gene targeted metagenomics.

Nurdiani D, Ito M, Maruyama T, Terahara T, Mori T, Ugawa S, Takeyama H

Journal of Bioscience and Bioengineering 2015-

Self-oriented immobilization of DNA polymerase tagged by titanium-binding peptide motif.

Nishida H, Kajisa T, Miyazawa Y, Tabuse Y, Yoda T, Takeyama H, Kambara H, Sakata T

langmuir 31p.732 - 7402015-

Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes.

Hosokawa M, Hoshino Y, Nishikawa Y, Hirose T, Yoon DH, Mori T, Sekiguchi T, Shoji S, Takeyama H

Biosensors and Bioelectronics 67p.379 - 3852015-

Draft Genome Sequence of Falsirhodobacter sp. Strain alg1, an Alginate-Degrading Bacterium Isolated from Fermented Brown Algae.

Mori T, Takahashi M, Tanaka R, Shibata T, Kuroda K, Ueda M, Takeyama H

Genome Announcement 22014-

Whole Genome Analyses of Marine Fish Pathogenic Isolate, Mycobacterium sp. 012931.

Kurokawa S, Kabayama J, Hwang SD, Nho SW, Hikima JI, Jung TS, Kondo H, Hirono I, Takeyama H, Mori T, Aoki

Marine biotechnology 16p.572 - 5792014-

In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy

Miyaoka R, Hosokawa M, Ando M, Mori T, Hamaguchi H, Takeyama H

Marine drugs (12) p.2827 - 28392014-

An environmental bacterial taxon with a large and distinct metabolic repertoire

Wilson MC, Mori T, Rckert C, Uria AR, Takada K, Gernert C, Steffens U, Heycke N, Schmitt S, Rinke C, Gurgui C, Helf MJ, Helfrich EN, Wakimoto T, Kracht M, Semeniuk A, Crsemann M, Hentschel U, Abe I, Matsunaga S, Kalinowski J, Takeyama H, Piel J

Nature (506) p.58 - 622014-

Comparative Genomic Characterization of Three Streptococcus parauberis Strains in Fish Pathogen, as Assessed by Wide-Genome Analyses

Nho S-W, Hikima J-I, Park S Bin, Jang H Bin, Cha IS, Yasuike M, Nakamura Y, Fujiwara A, Sano M, Kanai K, Kondo H, Hirono I, Takeyama H, Aoki T, Jung T-S

PloS one (8) p.e803952013-

Sensitive Detection of Measles Virus Infection in the Blood and Tissues of Humanized Mouse by One-step Quantitative RT-PCR

Ikeno S, Suzuki M, Muhsen M, Ishige M, Kobayashi M, Ohno S, Takeda M, Nakayama T, Morikawa Y, Terahara K, Okada S, Takeyama H, Tsunetsugu-Yokota Y

2013-

Whole-Genome Sequence of Fish Pathogenic Strain Mycobacterium sp. 012931, Isolated from Yellowtail (Seriola quinqueradiata)

Kurokawa S, Kabayama J, Nho S-W, Hwang S-D, Hikima J, Jung T-S, Kondo H, Hirono I, Takeyama H, Aoki T

Genome Announcements (1) p.1 - 22013-

Comparative Sequence Analysis of a Multidrug-Resistant Plasmid from Aeromonas hydrophila

Del Castillo CS, Hikima J, Jang H-B, Nho S-W, Jung T-S, Wongtavatchai J, Kondo H, Hirono I, Takeyama H, Aoki T

Antimicrobial Agents and Chemotherapy (57) p.120 - 1292013-

Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Kurokawa S, Kabayama J, Fukuyasu T, Hwang SD, Park C-I, Park S-B, Del Castillo CS, Hikima J-I, Jung T-S, Kondo H, Hirono I, Takeyama H, Aoki T

Marine biotechnology (New York, N.Y.) (15) p.340 - 3482013-

Variable domain antibodies specific for viral hemorrhagic septicemia virus ( VHSV ) selected from a randomized IgNAR phage display library

Ohtani M, Hikima J, Jung T, Kondo H, Hirono I, Takeyama H

Fish & Shellfish Immunology (34) p.724 - 7282013-

LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus

Hikima J-I, Yi M-K, Ohtani M, Jung CY, Kim YK, Mun JY, Kim YR, Takeyama H, Aoki T, Jung TS

PLoS ONE (7) p.e515222012-

Determining the Diet of Larvae of Western Rock Lobster (Panulirus cygnus) Using High-Throughput DNA Sequencing Techniques

Richard O’Rorke1, Shane Lavery, Seinen Chow, Haruko Takeyama, Peter Tsai, Lynnath E. Beckley, Peter A. Thompson, Anya M. Waite, Andrew G. Jeffs

PLoS One (7) p.e427572012-

Immunochromatographic assay of cadmium levels in oysters

Nishi K, Kim I-H, Itai T, Sugahara T, Takeyama H, Ohkawa H

Talanta (97) p.262 - 2662012-

Investigation on Natural Diets of Larval Marine Animals Using Peptide Nucleic Acid-Directed Polymerase Chain Reaction Clamping.

Chow S, Suzuki S, Matsunaga T, Lavery S, Jeffs A, Takeyama H.

Mar Biotechnol (NY) (13) p.305 - 3132011-

Metagenomic Analysis of 0.2-μm-Passable Microorganisms in Deep-Sea Hydrothermal Fluid.

Nakai R, Abe T, Takeyama H, Naganuma T.

Mar Biotechnol (NY) (13) p.900 - 9082011-

Efficiency of peptide nucleic acid-directed PCR clamping and its application in the investigation of natural diets of the Japanese eel leptocephali

Terahara T, Chow S, Kurogi H, Lee S, Tsukamoto K, Mochioka N, Tanaka H, Takeyama H.

PLoS One (6) 2011-

Isolation and characterization of a GDSL esterase from the metagenome of a marine sponge-associated bacteria.

Y. Okamura, T. Kimura, H. Yokouchi, M. Meneses-Osorio, M. Kato, T. Matsunaga, H. Takeyama

Mar. Biotechnol (12) p.395 - 4022011-

High-Density Microcavity Array for Cell Detection: Single-Cell Analysis of Hematopoietic Stem Cells in Peripheral Blood Mononuclear Cells

M.Hosokawa, A. Arakaki, M. Takahashi, T. Mori, H. Takeyama, T.Matsunaga

Anal. Chem. (81) p.5308 - 53132009-

Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria

H. Nakazawa, A.Arakaki, S. Narita-Yamada, I. Yashiro, K.Jinno, N. Aoki, A. Tsuruyama, Y. Okamura, S.Tanikawa, N. Fujita, H. Takeyama, T. Matsunaga

Genome Res. (19) p.1801 - 18082009-

Magnetic Separation of Human Podocalyxin-Llike Protein 1 (hPCLP1)-Positive Cells from Peripheral Blood Using Anti-hPCLP1 Monoclonal Antibody and Protein A Expressed on Bacterial Magnetic Particles

M. Kuhara, T. Yoshino, M. Shiokawa, T. Okabe, S. Mizoguchi, A. Yabuhara, H. Takeyama, T.Matsunaga

Cell Struct. Funct. (34) p.23 - 302009-

Nano-sized bacterial magnetic particles displaying pyruvate phosphate dikinase for pyrosequencing

T. Yoshino, T.Nishimura, T. Mori, S. Suzuki, H. Kambara, H. Takeyama, T. Matsunaga,

Biotechnol. Bioeng. 103p.130 - 1372009-

A stable human progesterone receptor expressing HeLa reporter cell line as a tool in chemical evaluation at the different cell-cycle phases.

T. Mori, M. Murata, T. Yoshino, S. Nakasono, F. Saito, H. Takeyama, T. Matsunaga

Toxicol. Lett. 186p.123 - 1292009-

Direct Magnetic Separation of Immune Cells from Whole Blood Using Bacterial Magnetic Particles Displaying Protein G.

M. Takahashi, T. Yoshino, H. Takeyama, T. Matsunaga

Biotechnol. Prog 25(1) p.219 - 2262009-

Preliminary Analysis of Length and GC Content Variation in the Ribosomal First Internal Transcribed Spacer (ITS1) of Marine Animals

S. Chow, Y. Ueno, M. Toyokawa, I. Oohara, H. Takeyama

Mar. Biotechnol. 2008/10-

Molecular diet analysis of phyllosoma larvae of the Japanese spiny lobster Panulirus japonicus (Decapoda: Crustacea)

N.Suzuki, K.Hoshino, K. Murakami, H.Takeyama, S.Chow

Marine Biotechnol. (10) p.49 - 552008-

Magnetic Cell Separation Using Nano-sized Bacterial Magnetic Particles with Reconstructed Magnetosome Membrane

T. Yoshino, H. Hirabe, M. Takahashi, M. Kuhara, H. Takeyama, Ta. Matsunaga

Biotechnol. Bioeng. (101) p.470 - 4772008-

Development and Application of a Stable HeLa Cell Line Capable of Site-Specific Transgenesis Using the Cre-lox System: Establishment and Application of a Stable TNFRI Knockdown Cell Line to Cytotoxicity Assay

F. Saito, H. Yokota, Y. Sudo, Y. Yakabe, H. Takeyama, T. Matsunaga

Toxicology In Vitro (22) p.1077 - 10872008-

Novel Method for Evaluation of Chemicals Based on Ligand-Dependent Recruitment of GFP Labeled Coactivator to Estrogen Receptor Displayed on Bacterial Magnetic Particles

T. Yoshino, C. Kaji, M. Nakai, F. Saito, H. Takeyama, T. Matsunaga

Anal.Chim.Acta, (626) p.71 - 772008-

Development of Cell Surface Display System in a Magnetotactic Bacterium, Magnetospirillum magneticum AMB-1

M. Tanaka, Y. Nakata, T. Mori, Y. Okamura, H. Miyasaka, H. Takeyama, T. Matsunaga

Appl. Environ. Microbiol. (74) p.3342 - 33482008-

Cellular Response to Electrochemical Killing Process by Applying a Constant Potential in Synchronously Cultured Saccharomyces cerevisiae.

H. Takeyama, T. Suzuki, Y. Okamura, T. Mori, T. Matsunaga

Electrochemistry (76) p.603 - 6052008-

High-Efficiency Single-Cell Entrapment and Fluorescence in Situ Hybridization Analysis Using a Poly(dimethylsiloxane) Microfluidic Device Integrated with a Black Poly(ethylene terephthalate) Micromesh.

T. Matsunaga, M. Hosokawa, A. Arakaki, T. Taguchi, T. Mori, T. Tanaka, H. Takeyama:

Anal. Chem. (80) p.5139 - 51452008-

Reporter Gene Assay against Lipophilic Chemicals Based on Site-Specific Genomic Recombination of a Nuclear Receptor Gene, Its Response Element, and a Luciferase Reporter Gene within a Stable HeLa Cell Line.

T. Mori, F. Saito, T. Yoshino, H. Takeyama, T. Matsunaga

Biotechnol. Bioeng. (99) p.1453 - 14612008-

Eukaryotes from the hepatopancreas of lobster phyllosoma larvae

N. Suzuki, K.Murakami, H.Takeyama, S.Chow

Bull. Fish. Res. Agen. (20) p.1 - 72007-

Determination of microsatellite repeats in the human thyroid perocidase (TPOX) gene using an automated gene analysis system with nanoscale engineered Biomagnetite

T. Nakagawa, K. Maruyama, H. Takeyama, T. Matsunaga

Biosens. Bioelectron (22) p.2276 - 22812007-

Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) with a fully automated system using a nano-scale engineered biomagnetite

K. Maruyama, H. Takeyama, T. Mori, K. Ohshima, S. Ogura, T. Mochizuki, T. Matsunaga:

Biosens. Bioelectron. (22) p.2282 - 22882007-

High-throughput SNP detection using nano-scale engineered biomagnetite"

T. Matsunaga, K.Maruyama, H. Takeyama, T. Katoh

Biosens. Bioelectron. (22) p.2315 - 23212007-

Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

T. Suzuki, Y. Okamura, A. Arakaki, H. Takeyama, T. Matsunaga

FEBS Lett (581) p.3443 - 34482007-

Fully automated immunoassay for detection of prostate-specific antigen using nano-magnetic beads and micro-polystyrene bead composites, 'Beads on Beads'

T. Matsunaga, Y. Maeda, T. Yoshino, H. Takeyama, M. Takahashi, H. Ginya, J. Asahina, H. Tajima

Anal. Chim. Acta, (597) p.331 - 3392007-

Detection of Cryptosporidium parvum oosysts using a microfluidic device equipped with the SUS micromesh and FITC-labeled antibody

T. Taguchi, A. Arakaki, H. Takeyama, S. Haraguchi, M. Yoshino, M. Kaneko, Y. Ishimori, T. Matsunaga:

Biotechnol. Bioeng. (96) p.272 - 2802007-

Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae

S. Ohkudo, H. Miyashita, A. Murakami, H. Takeyama, T. Tsuchiya, M. Mimura

Appl. Environm. Microbiol. (72) p.7912 - 79152006-

A molecular attempt to identify prey organisms of lobster phyllosoma larvae". Fisheries Science, 72, 342-349 (2006)

N. Suzuki, K.Murakami, H.Takeyama, S.Chow:

Fisheries Science (72) p.342 - 3492006-

Astaxanthin formation in the marine photosynthetic bacterium Rhodomulum sulfidophilum expressing crtI, crtY, crtW and crtZ" FEMS Micobiol. Lett., 265, 69-75 (2006)

D. Mukoyama, H. Takeyama, Y. Kondo, T. Matsunaga:

FEMS Micobiol. Lett. (265) p.69 - 752006-

Magnetic separation of CD14+ cells using antibody binding with protein A expressed on bacterial magnetic particles for generating dendritic cells" Biochem. Biophys. Res. Commun., 350, 1019-1025 (2006)

T. Matsunaga, M. Takahashi, T. Yoshino, M. Kuhara, H. Takeyama;

Biophys. Res. Commun. (350) p.1019 - 10252006-

Catechol Shiderophore Excretion by the Magnetotactic Bacterium Magnetospirillum magneticum AMB-1

R. J. Calugay, H. Takeyama, D. Mukoyama, Y. Fukuda, T. Suzuki, K. Kanoh, T. Matsunaga:

Biosci. Bioeng. (101) p.445 - 4472006-

Global Gene Expression Analysis of Iron-Inducible Genes in Magnetospirillum magneticum AMB-1

T. Suzuki, Y. Okamura, R. J. Calugay, H. Takeyama, T. Matsunaga:

J. Bacteriol. (188) p.2275 - 22792006-

Simultaneous detection of multiple mutations conferring streptomycin resistance in Mycobacterium tuberculosis using nano-scale engineered biomagnetites

K. Maruyama, N. Uchida, H. Takeyama, T. Mori, R. Kawaguchi, T. Matsunaga:

Nanobiotechnol. (2) p.71 - 782006-

Application of RNAi inducible TNFRI knockdown cells to the analysis of TNF induced cytotoxicity

F. Saito, H. Yokota, Y. Sudo, Y. Yakabe, H. Takeyama, T. Matsunaga:

Toxicol. In Vitro (20) p.1343 - 43532006-

Discrimination of DNA mismatches by direct force measurement for identification of Tuna species

T. Tanaka, T. Sasaki, Y. Amemiya, H. Takeyama, S. Chow, T. Matsunaga:

Anal. Chim. Acta (561) p.150 - 1552006-

Dynamic Analysis of a Genomic Island in Magnetospirillum sp. Strain AMB-1 Reveals how Magnetosome Synthesis Developed

Y. Fukuda, Y. Okamura, H. Takeyama, T. Matsunaga:

FEBS Lett. (580) p.801 - 8122006-

Origin of magnetosome membrane: proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane

M. Tanaka, Y. Okamura, A. Arakaki, T. Tanaka, H. Takeyama, T. .Matsunaga:

Proteomics (6) p.5234 - 52472006-

Direct counting of Cryptosporidium parvum oocysts using fluorescence in situ hybridizaition on a membrane filter

T. Taguchi, Y. Shinozaki, H. Takeyama, S. Haraguchi, M. Yoshino, M. Kaneko, Y. Ishimori, T. Matsunaga:

J. Microbiol. Meth. (67) p.373 - 3802006-

Phylogenetic relationships among Thunnus tuna species inferred from rDNA ITS1 sequence.

S. Chow , T. Nakagawa, N. Suzuki, H. Takeyama, , T. Matsunaga,

J. Fish Biol. , (68) p.24 - 352006-

Whole Metagenome Amplification of a Microbial Community Associated with Scleractinian Coral by MDA using 29 DNA polymerase

H. Yokouchi, Y. Fukuoka, D. Mukoyama, R. Calugay, H. Takeyama, T. Matsunaga:

Environ. Microbiol. (8) p.1155 - 11632006-

Capture and Release of DNA Using Aminosilane-Modified Bacterial Magnetic Particles for Automated Detection System of Single Nucleotide Polymorphisms

T. Nakagawa, R. Hashimoto, K. Maruyama, T. Tanaka, H. Takeyama and T. Matsunaga:

Biotechnol. Bioeng. (94) p.862 - 8682006-

Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology

T. Tanaka, K. Hatakeyama, M. Sawaguchi, A. Iwadate, Y. Mizutani, K. Sasaki, N. Tateishi, H. Takeyama, T. Matsunaga:

Biotechnol. Bioeng (95) p.22 - 282006-

Fabrication an amino silane coated microchip for DNA extraction from whole blood

T. Nakagawa, T. Tanaka, D. Niwa, T. Osaka, H. Takeyama, T. Matsunaga:

J. Biotechnol., (116) p.105 - 1112005-

Complete Genome Sequence of the Facultative Anaerobic Magnetotactic Bacterium Magnetospirillum sp. strain AMB-1.

T. Matsunaga, Y. Okamura, Y. Fukuda, A. T. Wahyudi, Y. Murase, H. Takeyama

DNA Research (12) p.157 - 1662005-

Magnetic nanotube fabrication by using magnetic nanocrystals

I. A. Banerjee, L. Yu, M. Shima, T. Yoshino, H. Takeyama, T. Matsunaga, H. Matsui:

Advanced Materials (17) p.1128 - 11312005-

Immuno-Capture of Cryptosporidium parvum Using Micro-Well Array

T. Taguchi, H. Takeyama, M. Tadashi:

Biosens. Bioelectron. (20) p.2276 - 22822005-

Development of a Novel Method for Screening of Estrogenic Compounds Using Nano-Sized Bacterial Magnetic Particles Displaying Estrogen Receptor

T. Yoshino, F. Kato, H. Takeyama, M. Nakai, Y. Yakabe, T. Matsunaga:

Anal. Chim. Acta (532) p.105 - 1112005-

"Marine microalgae"

T. Matsunaga, H. Takeyama, H. Miyashita, H. Yokouchi

Adv. Biochem. Engin. / Biotechnol. (96) p.165 - 1882005-

Rapid and Sensitive Detection of 17β-estradiol in Environmental Water Using Automated Immunoassay System with Bacterial Magnetic Particles." J. Biotechnol., 108, 153-159 (2004)

T. Tanaka, H. Takeda, F. Ueki, K. Obata, H. Tajima, H. Takeyama, Y. Goda, S. Fujimoto, T. Matsunaga:

J. Biotechnol. (108) p.153 - 1592004-

Comparative Phylogenetic Analyses of Halomonas variabilis and related organisms based on 16S rRNA, gyrB and ectBC gene sequences

T. Okamoto, A. Maruyama, S. Imura, H. Takeyama, T. Naganuma:

Appl. Microbiol (27) p.323 - 3332004-

Siderophore production of a periplasmic transport binding protein kinase gene defective mutant of Magnetospirillum magneticum AMB-1

R. J. Calugay, Y. Okamura, A. Tri Wahyudi, H. Takeyama, T. Matsunaga

Biochem. Biophys. Res. Commun. (323) p.852 - 8572004-

Intragenomic sequence variation in 18S rDNA of the symbiotic Dinoflagellate, the genus Symbiodinium from Porites lutea

H. Yokouchi, H. Takeyama, H. Taniguchi, M. Omori, T. Matsunaga:

Mar. Biotechnol., (6) p.5294 - 52992004-

Assembly of G protein-coupled receptors onto nanosized bacterial magnetic particles using Mms16 as an anchor molecule

T. Yoshino, M. Takahashi, H. Takeyama, Y. Okamura, F. Kato, T. Matsunaga:

Appl. Environ. Microbiol. (70) p.2880 - 28852004-

Magnetic cell using antibody binding with protein A expressed on bacterial magnetic particles

M. Kuhara, H. Takeyama, T. Tanaka, T. Matsunaga:

Anal. Chem. (76) p.6207 - 62132004-

Single nucleotide polymorphism detection in aldehyde dehydrogenase 2 (ALDH2) gene using bacterial magnetic particles based on dissociation curve analysis

K. Maruyama, H. Takeyama, E. Nemoto, T. Tanaka, K. Yoda, T. Matsunaga:

Biotechnol. Bioeng (87) p.687 - 6942004-

Single nucleotide polymorphism genotyping of aldehyde dehydrogenase 2 gene using a single bacterial magnetic particle

T. Yoshino, T. Tanaka, H. Takeyama, T. Matsunaga:

Biosens. Bioelectron (18) p.661 - 6662003-

SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles." Biosens. Bioelectron. 18, 683-687 (2003)

H. Ohta, H. Takeyama, H. Nakayama, T. Katoh, T. Matsunaga

Biosens. Bioelectron. (18) p.683 - 6872003-

Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles." Biosens. Bioelectron., 19, 325-330 (2003)

T. Tanaka, K. Maruyama, K. Yoda, E. Nemoto, Y. Udagawa, H. Nakayama, H. Takeyama, T. Matsunaga

Biosens. Bioelectron. (19) p.325 - 3302003-

Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle

H. Ota, A. Arakaki, T. Tanaka, H. Takeyama T. Matsunaga:

Biomol. Engineering (20) p.305 - 3092003-

Characterization of aldehyde ferredoxin oxidoreductase gene defective mutant in Magnetospirillum magneticum AMB-1

A. T. Wahyudi, H. Takeyama, Y.Okamura, Y. Fukuda, Tadashi Matsunaga

Biochem. Biophysic. Res. Comm. (303) p.223 - 2292003-

Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles." Anal. Chim. Acta, 475, 75-83 (2003).

T. Matsunaga, F. Ueki, K. Obata, H. Tajima, T. Tanaka, H. Takeyama, Y. Goda, S. Fujimoto

Anal. Chim. Acta, 475, 75-83 (2003). (475) p.75 - 832003-

In situ identification of symbiotic dinoflagellates, the genus symbiodinium with fluorescence-labeled rRNA-targeted oligonucleotide probes

H. Yokouchi, H. Takeyama, H. Miyashita, T. Maruyama, T. Matsunaga

J. Microbiol. Methods (53) p.327 - 3342003-

Design and Application of a New Cryptic-Plasmid-Based Shuttle Vector for Magnetospirillum magneticum

Y. Okamura, H. Takeyama, T. Sekine, T. Sakaguchi, A. T. Wahyudi, R. Sato, S. Kamiya, T. Matsunaga

Appl. Environ. Microbiol. (69) p.4274 - 42772003-

Single-nucleotide polymorphism analysis using fluorescence resonance energy transfer between DNA-labeling fluorophore, fluorescence isothiocyanate, and DNA intercalator, POPO-3, on bacterial magnetic particles

H. Nakayama, A. Arakaki, K. Maruyama, H. Takeyama, T. Matsunaga:

Biotechnol. Bioeng. (84) p.96 - 1022003-

Fully automated DNA extraction from blood using magnetic particles modified with hyperbranched polyamidoamine dendrimer " J. Biosci. Bioeng., 95, 21-26 (2003)

B. Yoza, A. Arakaki, K. Maruyama, H. Takeyama, T. Matsunaga

J. Biosci. Bioeng. (95) p.216 - 262003-

Screening of buoyant marine cyanobacteria using sucrose gradient centrifugation" J. Appl. Phycol., 14, 91-95 (2002)

M. Matsumoto, H. Miyashita, H. Takeyama, T. Matsunaga

J. Appl. Phycol. (14) p.91 - 952002-

Screening of soil bacteria for production of biocleaner" Appl. Biochem. Biotechnol., 98/100, 319-326 (2002)

H. Takeyama, M. Wada, T. Matsunaga

Appl. Biochem. Biotechnol. 98(100) p.319 - 3262002-

Cadmium recovery by a sulfate-reducing magnetic bacterium, Desulfovibrio magneticums RS-1 using magnetic separation" Appl. Biochem. Biotechnol., 98/100, 833-840 (2002)

A. Arakaki, H. Takeyama, T. Tanaka, T. Matsunaga

Appl. Biochem. Biotechnol. 98(100) p.833 - 8402002-

16 rRNA-Targeted identification of cyanobacteria genera using oligonucleotide-probes immobilized on bacterial magnetic particles" J. Appl. Phycol., 13, 389-394 (2001)

T. Matsunaga, H. Takeyama, H, Nakayama

J. Appl. Phycol。 (13) p.389 - 3942001-

A magnetosome-specific GTPase from the magnetic bacterium Magnetospirillum magneticum AMB-1" J.Biol.Chem., 276, 48183-48188 (2001)

Y. Okamura, H. Takeyama, T. Matsunaga

J.Biol.Chem., 276, 48183-48188 (2001) (276) p.48183 - 481882001-

Effects growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1" Enzyme Microbioal Technol., 29, 13-19 (2001)

C.-D. Yang, H. Takeyama, T. Tanaka, T. Matsunaga

Enzyme Microbioal Technol. (29) p.139 - 192001-

Mitochondrial DNA sequence variation within and between tuna Thunnus species and its application to species identification" J. Fish Biol., 58, 1646-1657 (2001)

H. Takeyama, S. Chow, H. Tsuzuki, T. Matsunaga

J. Fish Biol. (58) p.1646 - 16572001-

Single nucleotide polymorphism analysis using a bacterial magnetic particle microarray" Electrochemistry, 69, 1008-1012 (2001)

T. Yoshino, H. Takeyama, T. Matsunaga

Electrochemistry (69) p.1008 - 10122001-

Fluorescent detection of cyanobacterial DNA using bacterial magnetic particles on a MAG-microarray" Biotechnol. Bioeng., 73, 400-405 (2001)

T. Matsunaga, H. Nakayama, M. Okochi, H. Takeyama

Biotechnol. Bioeng. (73) p.400 - 4052001-

Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture." J. Biosci. Bioeng., 91, 213-216 (2001)

C.-D. Yang, H. Takeyama, T. Matsunaga

J. Biosci. Bioeng. (91) p.213 - 2162001-

Development of a high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system" Appl. Biochem. Biotechnol., 91-93, 109-116 (2001)

R. Sato, H. Takeyama, T. Tanaka, T. Matsunaga

Appl. Biochem. Biotechnol. 91(93) p.109 - 1162001-

Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particles synthesis by transposon mutagenesis" Appl. Biochem. Biotechnol., 91-93, 147-154 (2001)

A. T. Wahyudi, H. Takeyama, T. Matsunaga

Appl. Biochem. Biotechnol. 91(93) p.147 - 1542001-

Synthesis of bacterial magnetic particles during cell cycle of Magnetospirillum magneticum AMB-1" Appl. Biochem. Biotechnol., 91-93, 155-160 (2001)

C.-D. Yang, H. Takeyama, T. Tanaka, A. Hasegawa, T. Matsunaga

Appl. Biochem. Biotechnol. 91(93) p.155 - 1602001-

Discrimination between Atlantic and Pacific subspecies of the northern bluefin tuna (Thunnus thynnus) by magnetic-capture hybridization using bacterial magnetic particles" Mar. Biotechnol., 2, 309-313 (2000)

H. Takeyama, H. Tsuzuki, S. Chow, H. Nakayama, T. Matsunag

Mar. Biotechnol. (2) p.309 - 3132000-

Two Tandemly arrayed transfer-RNA-derived SINEs of the Medaka (Oryzias latipes)" Mar. Biotechnol., 2, 399-403 (2000)

N. Amano, H. Takeyama, T. Kusama, M. Sakaizumi, T. Oshiro, T. Matsunaga

Mar. Biotechnol. (2) p.399 - 4032000-

Cloning and characterization of a gene, mpsA, encoding a protein associated with intracellular magnetic particles from Magnetospirillum sp. strain AMB-1." Biochem. Biophysic. Res. Comm., 268, 932-937 (2000)

T. Matsunaga, N. Tsujimura, Y. Okamura, H. Takeyama

Biochem. Biophysic. Res. Comm. (268) p.932 - 9372000-

Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp." Lipids, 35, 1061-1064 (2000)

R. Yu, A. Yamada, K. Watanabe, K. Yazawa, H. Takeyama, T. Matsunaga, R. Kurane

Lipids (35) p.1061 - 10642000-

Nuclear and mitochondrial DNA analyses reveal four genetically separated breeding units of the swordfish ." J. Fish Biol., 56, 1087-1098 (2000)

S. Chow, H. Takeyama

J. Fish Biol. (56) p.1087 - 10982000-

Electrochemical killing of Streptococcus mutans causing dental caries" Electrochemistry

H. Yokouchi, M. Okochi, H. Takeyama, T. Matsunaga:

Electrochemistry (68) p.875 - 8772000-

Screening of marine microalgae for bioremediation of cadmium-polluted seawater." J. Biotechnol., 70, 33-38 (1999)

T. Matsunaga, H. Takeyama, T. Nakao, A. Yamazawa

J. Biotechnol. (70) p.33 - 382000-

Two-dimensional analysis of proteins specific to the bacterial magnetic particles membrane from Magnetospirillum sp. AMB-1." Appl. Biochem. Biotechnol., 84-86, 441-446 (2000)

Y. Okamura, H. Takeyama, T. Matsunaga

Appl. Biochem. Biotechnol. 84(86) p.441 - 4462000-

Salinity-Regulated Replication of an endogenous plasmid pSY10 from the marine cyanobacterium Synechococcus sp." Appl. Biochem. Biotechnol. 84-86, 447-453 (2000)

H. Takeyama, H. Nakayama, T. Matsunaga

Appl. Biochem. Biotechnol. 84(86) p.447 - 4532000-

Floating cultivation of marine cyanobacteria using coal fly ash." Appl. Biochem. Biotechnol., 84-86, 51-57 (2000)

M. Matsumoto, E. Yoshida, H. Takeyama, T. Matsunaga

Appl. Biochem. Biotechnol. 84(86) p.51 - 572000-

Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex" J. Magn. Magn. Mater., 194, 126-131 (1999)

T. Matsunaga, R. Sato, S. Kamiya, T. Tanaka, H. Takeyama

J. Magn. Magn. Mater. (194) p.126 - 1311999-

"Analysis of stress responsive gene for salinity in marine cyanobacterium Synechococcus sp."

H. Takeyama, H. Nakayama

New development in marine biotechnology p.255 - 2571998-

"Marine genome"

T. Matsunaga, H. Takeyama

BioHydrogen p.31 - 381998-

"Production of useful materials from marine microalgae"

H. Takeyama, T. Matsunaga

Cyanobacterial Biotechnology p.329 - 3401998-

"Marine genome technology"

T. Matsunaga, H. Takeyama

Marine microorganisms for industry p.48 - 551998-

UV-A-induced expression of a GroEL in the UV-A-resistant marine cyanobacterium Oscillatoria sp." Microbiology, 145, 949-954 (1999)

A. Yamazawa, H. Takeyama, D. Takeda, T. Matsunaga

Microbiology (145) p.949 - 9541998-

"Microalgae producing useful metabolites"

T. Matsunaga, H. Takeyama

Biofutur (179) p.40 - 431998-

Intron length variation observed in the creatine kinase and ribosomal protein genes of the swordfish Xiphias gladius." Fish. Sci., 64, 397-402 (1998)

S. Chow, H. Takeyama

Fish. Sci. (64) p.397 - 4021998-

Genetic stock structure of the swordfish (Xiphias gladius) inferred by PCR-RFLP analysis of the mitochondrial DNA control region." Mar. Biol., 127, 359-367 (1997)

S. Chow, H. Okamoto, Y. Uozumi, Y. Takeuchi, H. Takeyama

Mar. Biol. (127) p.359 - 3671997-

Expression of eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp." Microbiology, 143, 2725-2731 (1997)

H. Takeyama, D. Takeda, K. Yazawa, A. Yamada, T. Matsunaga

Microbiology (143) p.2725 - 27311997-

Cloning, sequencing and expressing the carotenoid biosynthesis genes, lycopene cyclase and phytoene desaturase, from the aerobic photosynthetic bacterium Erythrobacter longus sp. strain OCh 101 in Escherichia coli." Gene, 189, 169-174 (1997)

H. Matsumura, H. Takeyama, E. Kusakabe, J. G. Burgess, T. Matsunaga

Gene (189) p.169 - 1741997-

Production of antioxidant vitamins, -carotene, vitamin C and vitamin E, by the two-step cultured Euglena gracilis Z." Biotechnol. Bioeng., 53, 185-190 (1997)

H. Takeyama, A. Kanamaru, Y. Toshino, H. Kakuta, Y. Kawamura, T. Matsunaga

Biotechnol. Bioeng. (53) p.185 - 1901997-

DHA enrichment of rotifers:A simple two-step culture using unicellular algae Chlorella regularis and Isochrysis galbana" J. Mar. Biotechnol. 3, 244-247 (1996)

H. Takeyama, K. Iwamoto, S. Hata, T. Matsunaga

J. Mar. Biotechnol. (3) p.244 - 2471996-

β-carotene production in a novel hydrogen-producing marine photosynthetic bacterium Rhodovulum sulfidophilum expressing the Erythrobacter longus OCh101 crtI and crtY genes

H. Takeyama, J. Sunarjo, A. Yamada, H. Matsumura, E. Kusakabe, T. Matsunaga

J. Mar. Biotechnol. (4) p.224 - 2291996-

Recovery of a marine cyanobacterial recombinant product using fish-feed organisms.

K. Sode, T. Hayashi, M. Tatara, N. Hatano, H. Yoshida, H. Takeyama, T. Oshiro, T. Matsunaga

J. Mar. Biotechnol. (4) p.82 - 861996-

Phylogenic analysis of a novel sulfate-reducing magnetic bacterium, RS-1, demonstrates its membership of the -Proteobacteria

R. Kawaguchi, J. G. Burgess, T. Sakaguchi, H. Takeyama, T. Matsunaga

FEMS Microbiol. Lett. (126) p.277 - 2821995-

Screening of marine cyanobacteria for high palmitoleic acid production

T. Matsunaga, H. Takeyama, Y. Miura, T. Yamazaki, H. Furuya, K. Sode

FEMS Microbiol. Lett. (133) p.137 - 1411995-

"Genetic engineering of marine cyanobacteria."

T. Matsunaga, H. Takeyama

J. Appl. Phycol. (7) p.77 - 841995-

Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of marine cyanobacterium.

H. Takeyama, A. Yamazawa, C. Nakamura, T. Matsunaga

Biotechnol. Techniques (9) p.355 - 3601995-

Screening of melanin biosynthesis inhibitors from marine microalgae using Streptomyces bikiniensis bioassay

Y. Wachi, K. Sode, K. Horikoshi, H. Takeyama, T. Matsunaga

Biotechnol. Techniques (9) p.633 - 6361995-

Cyclic synthesis of messenger RNA from the nitrogenase nifH gene in synchronous culture of marine unicellular cyanobacterium, Synechococcus sp. strain Miami BG043511

C. Campbell, H. Takeyama, A. Mitsui

J. Mar. Biotechnol. (2) p.39 - 431994-

Sequence of 2.6 kb cryptic plasmid from a marine cyanobacterium Synechococcus sp

R. Kawaguchi, T. Nagaoka, J. G. Burgess, H. Takeyama, T. Matsunaga:

Plasmid, (32) p.245 - 2531994-

Conjugative gene transfer in marine cyanobacteria, Synechococcus sp., Synechocystis sp. and Pseudanabaena sp

K. Sode, M. Tatara, H. Takeyama, J. G. Burgess, T. Matsunaga:

Appl. Microbiol. Biotechnol. (37) p.369 - 3731992-

CO2 removal by high-density culture of a marine cyanobacterium Synechococcus sp. using an improved photobioreactor employing light- diffusing optical fibers.

H. Takano, H. Takeyama, N. Nakamura, T. Matsunaga

Appl. Biochem. Biotechnol. 34(35) p.449 - 4581992-

On-line monitoring of marine cyanobacterial cultivation based on phycocyanin fluorescence

K. Sode, K. Horikoshi, H. Takeyama, N. Nakamura, T. Matsunaga

J. Biotechnol. (21) p.209 - 2181991-

Salinity dependent copy number increase of a marine cyanobacterial endogenous plasmid.

H. Takeyama, J. G. Burgess, H. Sudo, T. Matsunaga

FEMS Microbiol. Lett. (90) p.95 - 981991-

Glutamate production from CO2 by marine cyanobacterium Synechococcus sp. using a novel biosolar reactor employing light-diffusing optical fibers

T. Matsunaga, H. Takeyama, H. Sudo, N. Oyama, S. Ariura, H. Takano, M. Hirano, J. G. Burgess, K. Sode, N. Nakamura

Appl. Biochem.Biotechnol. 28(9) p.157 - 1671991-

Characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmids potentially capable of transformation of marine cyanobacterium, Synechococcus sp., and its transformation

T. Matsunaga, H. Takeyama, N. Nakamura

Appl. Biochem. Biotechnol 24(5) p.151 - 1601990-

Exacerbation of invasive Candida albicans infection by commensal bacteria or a glycolipid through IFNγ produced in part by iNKT cells

Tarumoto N, Kinjo Y, Kitano N, Sasai D, Ueno K, Okawara A, Izawa Y, Shinozaki M, Watarai H, Taniguchi M, Takeyama H, Maesaki S, Shibuya K, Miyazaki Y

Journal of Infectious Disease

The complete genome sequence of Photobacterium damselae subsp. piscicida strain OT-51443 isolated from yellowtail (Seriola quinqueradiata) in Japan

2. Takashi Aoki, Yuki Teru, Natsuki Morimoto, Tomoya Kono, Masahiro Sakai, Tomokazu Takano, John Hawke, Yutaka Fukuda, Haruko Takeyama, and Jun-ichi Hikima,

genome announcements. p.2017

Books And Publication

「Springer Handbook of Marine Biotechnology」Se-Kwon Kim 編集(分担執筆:「Chapter 19: Marine Metagenome and Supporting Technology」の項)

Tetsushi Mori, Haruko Takeyama

Springer2015-

Detali

Responsible Number of Pages:497-508

“Metabolism and Innate Immunity: FOXO Regulation of Antimicrobial Peptides in Drosophila”

Loch G, Jentgens E, B_low M, Zinke I, Mori T, Suzuki S, Takeyama H, Hoch M

Krager2013-

Lecture And Oral

Exploitation of Useful Microbes Using Single Cell Technologies

H. Takeyama

2019/07/17

Access to Unculturable Environmental Microbes Using Advanced Technologies

H. Takeyama

2019/07/16

Marine Biotechnology: How to Get Treasure from the Ocean

H. Takeyama

2019/07/16

Marine microbiome analysis with the technologies for single-cell microbiology

H. Takeyama

Asia-Pacific Marine Biotechnology Conference 20172017/05/23

Detail

International conferenceOral presentation(general)Venue:Hawai

Droplet microfluidics for precise and high throughput whole genome amplification toward single-cell genome sequencing

Haruko Takeyama

microTAS 2016Invitation Yes2016/10/12

Detail

International conferenceOral presentation(invited, special)

Microbiome analysis: challenges in single cell technology

Haruko Takeyama

IMBC 2016Invitation Yes2016/09/01

Detail

International conferenceOral presentation(invited, special)

Analysis of marine invertebrate holobionts and supporting single-cell analysis technologies

Haruko Takeyama

The 8th International Workshop on Approaches to Single-Cell AnalysisInvitation Yes2014/09/10

Detail

International conferenceOral presentation(invited, special)

Metagenomic analysis of invertebrate holobionts and supporting technologies

Haruko Takeyama

The 10th Asia-Pacific Marine Biotechnology ConferenceInvitation Yes2014/05/06

Detail

International conferenceOral presentation(invited, special)

Treasure from the Ocean: Marine useful bio/genome resources

Haruko Takeyama

International Symposium on Halal Science and Innovative Product DevelopmentInvitation Yes2014/02/11

Detail

International conferenceOral presentation(invited, special)

Metagenomic Analysis of Invertebrate Holobionts and Their Application

Haruko Takeyama

The 16th Annual Conference of the Japanese Coral Reef SocietyInvitation Yes2013/12/12

Detail

National conferenceOral presentation(invited, special)

Metagenome analysis of marine invertebrate-associated bacteria and its supporting technologies

Haruko Takeyama

International Symposiium on Aquatic MetagenomicsInvitation Yes2013/11/23

Detail

International conferenceOral presentation(invited, special)

The way to single cell analysis

Haruko Takeyama

German-Japanese Joint Summer Symposium on Health Span DynamicsInvitation Yes2012/09/20

Detail

International conferenceOral presentation(invited, special)

Exploration and exploitation of Marine Uncultivable Microorganisms

Haruko Takeyama

The 9th Asia-Pacific Marine Biotechnology Conference2012/07/14

Detail

International conferenceOral presentation(keynote)

Analysis of informational biomacromolecules and their applications

Haruko Takeyama

The 6th Global COE International Symposium2011/12/11

Detail

International conferenceOral presentation(keynote)

Challenges in single cell analysis

Haruko Takeyama

Workshop Life Sciences Innovations & Biomedical ResearchInvitation Yes2011/12/07

Detail

International conferenceOral presentation(invited, special)

Treasure from the ocean: Marine metagenome resources and their application

Haruko Takeyama

International Union of Microbiological Societies 2011 CongressInvitation Yes2011/09/08

Detail

International conferenceOral presentation(invited, special)

Application of environmental genome resources and the single cell based technology

Haruko Takeyama

The 7th International Forum On Post-Genome TechnologiesInvitation Yes2011/02

Detail

International conferenceOral presentation(invited, special)

Patent

Reference Number:1585

食肉の加熱処理状況の判定方法、食肉の加熱処理状況の判定装置及びプログラム(日本)

濱口 宏夫, 安藤 正浩, 竹山 春子, 細川 正人

2014-231539、2016- 95230

Reference Number:2045

ウナギの卵を含有するメタボリックシンドロームの予防、抑制又は治療用の組成物(日本)

竹山 春子, 矢澤 一良, 高見澤 菜穂子, 青木 菜摘

2018-070737、2018-184386

Reference Number:2191

ゲルカプセル方式による1細胞ゲノムライブラリーからの配列スクリーニング法(日本)

細川 正人, 竹山 春子, 西川 洋平

2019-085829

Research Grants & Projects

Grant-in-aids for Scientific Research Adoption Situation

Research Classification:

Bioenrichment of useful but uncultivable microorganisms using marine invertebrates

2011/-0-2014/-0

Allocation Class:¥5330000

Research Classification:

Surveillance studies for promotion of the capacity building concerning Integrated Management of Chemicals

2010/-0-2013/-0

Allocation Class:¥17680000

Research Classification:

Use of Biosurfactants for Monitoring and Remediation of Persistent Organic Pollutants

Allocation Class:¥4680000

Research Classification:

Development of a pyrosequence-based technique for HIV drug-resistance diagnosis

Allocation Class:¥17550000

Research Classification:

Development and expansion of evaluation indicators for chemicals management in order to advance Capacity Building of Integrated Management of Chemicals

Allocation Class:¥14690000

Research Classification:

Simultaneous identification system for fisheries animals using ITS chip

Allocation Class:¥7140000

Research Classification:

The development of accurate quantitative and digital tools for Lifesurveyor

Allocation Class:¥300900000

Research Classification:

Nano-screening of marine symbiotic consortium for useful bio-materials and their analyses

Allocation Class:¥14900000

Research Classification:

Elucidation of Mechanisms for Biomagnetite Formation and Its Application

Allocation Class:¥568100000

Research Classification:

Application of buoyant marine cyanobacteriaon to eco-monitoring

Allocation Class:¥12900000

Research Classification:

Screening of enviromental stress response elements and their application

Allocation Class:¥3400000

Research Classification:

Analyses of physiological factors regulating oocyte maturation and early development of a hermaphrodite pond snail Lymnaea stagnalis

Allocation Class:¥2000000

Research Classification:

Molecular analysis of UV-A resistant operon in a marine cyanobacterium.

1998-2000

Allocation Class:¥12200000

Research Classification:

Construction of in vivo high-throughput screening system for bioactive metabolites

2014/-0-2016/-0

Allocation Class:¥3900000

Research Classification:

Development of technique for digital counting of DNA copy number at the single-cell level

2012/-0-2015/-0

Allocation Class:¥18980000

Research Classification:

Development of platform for ultra high-throughput screening of novel bioactive compound producers

2017/-0-2022/-0

Allocation Class:¥205010000

Research Classification:

Why does Fugu pufferfish have a resistance to bacterial infection? Research to seek the mechanism of disease resistance in Fugu.

2017/-0-2021/-0

Allocation Class:¥38740000

On-campus Research System

Special Research Project

単一細胞解析技術を用いたHIVの感染進行と成立の解析

2010

Research Results Outline:本研究では、二種類のHIV-1の細胞内での感染優位性の違いの解明を行うため、HIV-1のモデルとなるレンチウイルスの作成および単一細胞内の二種類のHI本研究では、二種類のHIV-1の細胞内での感染優位性の違いの解明を行うため、HIV-1のモデルとなるレンチウイルスの作成および単一細胞内の二種類のHIV-1の定量に向けた技術開発を行った。単一細胞レベルでのデジタルPCRを達成するためにCapill...本研究では、二種類のHIV-1の細胞内での感染優位性の違いの解明を行うため、HIV-1のモデルとなるレンチウイルスの作成および単一細胞内の二種類のHIV-1の定量に向けた技術開発を行った。単一細胞レベルでのデジタルPCRを達成するためにCapillary plate PCR (CP-PCR)を開発した。CP-PCRとは多数のポアがあるキャピラリープレート内で単一分子cDNA を個別・並列的に増幅する技術である。CP-PCRでは各ポア内のPCR 産物量がプライマーの数により制限され、各ポア内のPCR産物量が限られるという特徴を持つ。この特徴からPCR産物の濃度を定量することでテンプレート量の解析に繋げることができ、リアルタイムPCR で解析が可能になる。またCP-PCRを行うときにTaqManプローブを加え、PCR後に蛍光を発するポアをカウントすることで定量する方法を検討した。この方法では単一分子のテンプレートcDNAを単一蛍光ポアに置換することができ、微量のcDNAをカウントにより定量することが期待できる。 

環境有害金属のイムノクロマトグラフィーによる新規測定法の開発とその国際標準化

2010

Research Results Outline:カドミウム(Cd)は人体に有害な重金属であり、水道水基準値は3 µg/Lである。Cd濃度の分析法は高額な費用、長い測定時間、専門的な知識と技術、広い分カドミウム(Cd)は人体に有害な重金属であり、水道水基準値は3 µg/Lである。Cd濃度の分析法は高額な費用、長い測定時間、専門的な知識と技術、広い分析場所が必要であるため、発展途上国や試料採取場所では微量分析が期待できない状況であり、迅速で簡便な...カドミウム(Cd)は人体に有害な重金属であり、水道水基準値は3 µg/Lである。Cd濃度の分析法は高額な費用、長い測定時間、専門的な知識と技術、広い分析場所が必要であるため、発展途上国や試料採取場所では微量分析が期待できない状況であり、迅速で簡便な検出・定量法が求められていた。近年コメ中のCd濃度を迅速かつ簡便高感度に定量するイムノクロマトグラフィー(ImC)が開発された。しかしながらImCの定量下界は10 µg/Lで環境基準を分析するには測定段階前に前処理が必要であった。本研究では前処理カラムの保持力および緩衝液の緩衝能の改善などの前処理段階の条件を最適化しImC測定の高精度化および簡易化を実現した。

バクテリアシングルセルRNA-seqのためのドロップレットデバイスの開発

2015

Research Results Outline:1細胞トランスクリプトーム解析には、1細胞mRNAからcDNAを合成し、RNA-seqを実施可能とするための全転写産物増幅(WTA)の工程が必須である1細胞トランスクリプトーム解析には、1細胞mRNAからcDNAを合成し、RNA-seqを実施可能とするための全転写産物増幅(WTA)の工程が必須である。本研究では、微生物に特化した1細胞WTA法を開発することを目的とした。具体的には真核細胞用のWT...1細胞トランスクリプトーム解析には、1細胞mRNAからcDNAを合成し、RNA-seqを実施可能とするための全転写産物増幅(WTA)の工程が必須である。本研究では、微生物に特化した1細胞WTA法を開発することを目的とした。具体的には真核細胞用のWTA技術を改変して、Poly(A)鎖配列を持たないバクテリアのmRNAからのcDNA鎖合成と増幅について検討した。この結果、従来法が必要とするmRNA量よりも少ない鋳型量から増幅cDNAライブラリーを構築し、次世代シーケンサーにてトランスクリプトーム解析を実施できる可能性が示唆された。以上より、今後1細胞に対応するための基盤となる検討結果が得られた。

16S rRNA解析による魚介類の腸管および環境水の細菌叢の構造と役割の解明

2016

Research Results Outline:腸内の常在細菌叢は、外部からの病原体の影響を強く受け、侵入防御機構や腸管上皮細胞の分化誘導などの消化管免疫機能の役割を果たしている。魚類の腸内細菌叢(腸内の常在細菌叢は、外部からの病原体の影響を強く受け、侵入防御機構や腸管上皮細胞の分化誘導などの消化管免疫機能の役割を果たしている。魚類の腸内細菌叢(腸内フローラ)には多種多様な細菌叢が存在し、複雑な微生物生態系が形成しているが、その役割については...腸内の常在細菌叢は、外部からの病原体の影響を強く受け、侵入防御機構や腸管上皮細胞の分化誘導などの消化管免疫機能の役割を果たしている。魚類の腸内細菌叢(腸内フローラ)には多種多様な細菌叢が存在し、複雑な微生物生態系が形成しているが、その役割についてはほとんど解明されていない。そこで、本研究では、魚類の腸内フローラについて、メタゲノム解析を行い、腸内細菌の種類や組成を明らかにする事を目的とした。このために、今年度はサンプルからのDNA抽出法、16S rRNA遺伝子配列の解析に伴うプロトコルを検証し、今後の研究の基盤となる検討結果を得た。

Lecture Course

Course TitleSchoolYearTerm
Introduction to Bioscience A DenshibutsuriSchool of Fundamental Science and Engineering2020spring semester
Introduction to Bioscience A Kenchiku, keiei, shakou, shigenSchool of Creative Science and Engineering2020spring semester
Introduction to Bioscience B SeiiSchool of Advanced Science and Engineering2020spring semester
Science and Engineering Laboratory 1ASchool of Fundamental Science and Engineering2020spring semester
Science and Engineering Laboratory 1A VSchool of Fundamental Science and Engineering2020spring semester
Science and Engineering Laboratory 1ASchool of Creative Science and Engineering2020spring semester
Science and Engineering Laboratory 1A VSchool of Creative Science and Engineering2020spring semester
Science and Engineering Laboratory 1ASchool of Advanced Science and Engineering2020spring semester
Science and Engineering Laboratory 1A VSchool of Advanced Science and Engineering2020spring semester
Science and Engineering Laboratory 1B IIISchool of Fundamental Science and Engineering2020fall semester
Science and Engineering Laboratory 1B IIISchool of Creative Science and Engineering2020fall semester
Science and Engineering Laboratory 1B IIISchool of Advanced Science and Engineering2020fall semester
Introduction to Bioscience A SougoukikaiSchool of Creative Science and Engineering2020spring semester
Introduction to Bioscience A Kagaku, OukaSchool of Advanced Science and Engineering2020spring semester
Life Science and Medical Bio-science Seminar ISchool of Advanced Science and Engineering2020spring semester
Life Science and Medical Bio-science Seminar I [S Grade]School of Advanced Science and Engineering2020spring semester
MicrobiologySchool of Advanced Science and Engineering2020fall semester
Microbiology [S Grade]School of Advanced Science and Engineering2020fall semester
Life Science and Medical Bio-science Laboratory ISchool of Advanced Science and Engineering2020spring semester
Life Science and Medical Bio-science Laboratory I [S Grade]School of Advanced Science and Engineering2020spring semester
Analytical Chemistry BSchool of Advanced Science and Engineering2020fall semester
Analytical Chemistry B [S Grade]School of Advanced Science and Engineering2020fall semester
Life Science and Medical Bio-science Seminar IISchool of Advanced Science and Engineering2020fall semester
Life Science and Medical Bio-science Seminar II [S Grade]School of Advanced Science and Engineering2020fall semester
Graduation ResearchSchool of Advanced Science and Engineering2020full year
Graduation Research [S Grade]School of Advanced Science and Engineering2020full year
Bioinformatics: Exercise ISchool of Advanced Science and Engineering2020winter quarter
Infections and Defense Mechanisms in HumansSchool of Advanced Science and Engineering2020fall semester
BiostatisticsSchool of Advanced Science and Engineering2020fall quarter
Advanced Molecular MicrobiologySchool of Advanced Science and Engineering2020spring semester
Bioinformatics: Exercise 2School of Advanced Science and Engineering2020an intensive course(fall)
Marine Life Resources and EngineeringSchool of Advanced Science and Engineering2020fall quarter
Life Science and Medical Bio-science Laboratory IVSchool of Advanced Science and Engineering2020spring semester
Life Science and Medical Bio-science Laboratory VSchool of Advanced Science and Engineering2020fall semester
Life Science and Medical Bioscience Seminar IISchool of Advanced Science and Engineering2020spring semester
Bioscience Practicals ASchool of Advanced Science and Engineering2020fall semester
Bioscience Practicals BSchool of Advanced Science and Engineering2020spring semester
Graduation Thesis ASchool of Advanced Science and Engineering2020fall semester
Graduation Thesis BSchool of Advanced Science and Engineering2020spring semester
Laboratory for Advanced Science and Engineering ASchool of Advanced Science and Engineering2020spring quarter
Laboratory for Advanced Science and Engineering ASchool of Advanced Science and Engineering2020spring quarter
Laboratory for Advanced Science and Engineering ASchool of Advanced Science and Engineering2020spring quarter
Laboratory for Advanced Science and Engineering A [S Grade]School of Advanced Science and Engineering2020spring quarter
Laboratory for Advanced Science and Engineering A [S Grade]School of Advanced Science and Engineering2020spring quarter
Laboratory for Advanced Science and Engineering A [S Grade]School of Advanced Science and Engineering2020spring quarter
Laboratory for Advanced Science and Engineering BSchool of Advanced Science and Engineering2020summer quarter
Laboratory for Advanced Science and Engineering BSchool of Advanced Science and Engineering2020summer quarter
Laboratory for Advanced Science and Engineering BSchool of Advanced Science and Engineering2020summer quarter
Laboratory for Advanced Science and Engineering B [S Grade]School of Advanced Science and Engineering2020summer quarter
Laboratory for Advanced Science and Engineering B [S Grade]School of Advanced Science and Engineering2020summer quarter
Laboratory for Advanced Science and Engineering B [S Grade]School of Advanced Science and Engineering2020summer quarter
Current Topics in BiosciencesSchool of Advanced Science and Engineering2020fall semester
Current Topics in Biosciences [S Grade]School of Advanced Science and Engineering2020fall semester
Fundamental Bioscience LaboratorySchool of Advanced Science and Engineering2020spring semester
Life Science and Medical Bioscience LaboratorySchool of Advanced Science and Engineering2020spring semester
Advanced Bioscience SeminarSchool of Advanced Science and Engineering2020fall semester
Master's Thesis (Department of Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2020full year
Research on Biomolecular Science and EngineeringGraduate School of Advanced Science and Engineering2020full year
Research on Biomolecular Science and EngineeringGraduate School of Advanced Science and Engineering2020full year
Environmental BioresourcesGraduate School of Advanced Science and Engineering2020fall quarter
Advanced Biomolecular Science and Engineering (Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2020fall quarter
Advanced Biomolecular Science and Engineering (Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2020fall quarter
Seminar on Biomolecular Science and Engineering CGraduate School of Advanced Science and Engineering2020spring semester
Seminar on Biomolecular Science and Engineering CGraduate School of Advanced Science and Engineering2020spring semester
Seminar on Biomolecular Science and Engineering DGraduate School of Advanced Science and Engineering2020fall semester
Seminar on Biomolecular Science and Engineering DGraduate School of Advanced Science and Engineering2020fall semester
Master's Thesis (Department of Life Science and Medical Bioscience)Graduate School of Advanced Science and Engineering2020full year
Research on Biomolecular Science and EngineeringGraduate School of Advanced Science and Engineering2020full year
Practical Training for Career BuildingGraduate School of Advanced Science and Engineering2020full year
Integrated Management of Infectious DiseasesGraduate School of Advanced Science and Engineering2020an intensive course(spring)
Integrated Management of Infectious DiseasesGraduate School of Advanced Science and Engineering2020an intensive course(spring)
Planning and Research for Advanced Health ScienceGraduate School of Advanced Science and Engineering2020spring semester
Seminar on Advanced Health Science IGraduate School of Advanced Science and Engineering2020full year
Seminar on Advanced Health Science IIGraduate School of Advanced Science and Engineering2020full year
Seminar on Advanced Health Science IIIGraduate School of Advanced Science and Engineering2020full year
Practical Presentation IGraduate School of Advanced Science and Engineering2020full year
Practical Presentation IIGraduate School of Advanced Science and Engineering2020full year
Practical On-the-job TrainingGraduate School of Advanced Science and Engineering2020full year
Research on Life Science and Medical Bioscience TAKEYAMA, HarukoGraduate School of Advanced Science and Engineering2020full year
Introduction of Life Science 01Global Education Center2020spring semester
Introduction of Life Science 02Global Education Center2020spring semester
Introduction to the Ocean alphaGlobal Education Center2020fall quarter
Introduction to the Ocean betaGlobal Education Center2020winter quarter

Teaching Methods And Education And Presentation on Practice, Lectures, etc.

「Where the Sun Rises on Biotech:」

2005/12

Detail

Outline:Marine Biotechnology Seminar, St.John’s , Canada

「Automated Genotyping System Using Bio-Nanomagnetic Beads」

2005/05

Detail

Outline:2005 International Meeting of Microbiological Society of Korea, Korea

「Metagenomic approach to marine resources and support technology」

2005/05

Detail

Outline:Korea Ocean Research & Development Institute

「Exploitation of unculturable bioresources in marine environment」

2004/10

Detail

Outline:10th International Congress for Culture Collections, Tsukuba

「Challenge to exploration of useful gene resources from marine metagenome」

2004/05

Detail

Outline:Symposium for Progress in Marine Biotechnology in Korea and Japan In The 7th Japanese Society for Marine Biotechnology Conference, Hokkaidor

「Nano-biomagnetic formation and its application」

2003/11

Detail

Outline:Asia-Pacific International Molecular Biology Network 2003, The 6th Conference Dramatic Movement of Life Science: Now to Future, Tokyo

「Development of automated SNP detection system using nano biomagnetite」

2003/11

Social Contribution

読売新聞

2010/08-

日刊工業新聞

2009/04-

Bio Industry

2010/06-

Nature Digest

2009/07-

Nature Biotechnology Vol.27, Number 6

2009/06-

日刊工業新聞

2009/03-

YOMIURI ONLINE

2008/08-

化学と工業

2008/07-

日経BP

2007/07-

朝日新聞

2007/07-