氏名

セキグチ テツシ

関口 哲志

職名

(https://researchmap.jp/read0205964/) 上級研究員(研究院教授)

所属

(ナノ・ライフ創新研究機構)

連絡先

メールアドレス

メールアドレス
t-sekiguchi@waseda.jp
メールアドレス(その他)
t-sekiguchi@waseda.jp

URL等

研究者番号
70424819

本属以外の学内所属

学内研究所等

グリーンデバイス研究所

研究所員 2014年-2014年

グリーンデバイス研究所

研究所員 2010年-2013年

グリーンデバイス研究所

研究所員 2015年-

学歴・学位

学位

博士(工学) 課程 早稲田大学 電子・電気材料工学

所属学協会

化学センサ研究会

次世代センサ協議会

応用物理学会

電気化学協会

電気学会 E部門誌編集委員

日本生体医工学会

日本臨床工学技士会

日本麻酔学会

受賞

ケミナスポスターアワード

2011年06月

2019 Micromachines Best Paper Award

2020年02月授与機関:MDPI

タイトル:Size-Dependent and Property-Independent Passive Microdroplet Sorting by Droplet Transfer on Dot Rails

受賞者(グループ):D.H. Yoon, D. Tanaka, T. Sekiguchi, S. Shoji

研究分野

キーワード

micro fluidics、energy harvesting、medical electronics、chemical sensor

科研費分類

総合理工 / ナノ・マイクロ科学 / ナノマイクロシステム

総合理工 / ナノ・マイクロ科学 / 計測工学

研究シーズ

FeCo系合金の逆磁歪効果を用いた小型発電素子

シーズ分野:ナノ・材料, エネルギー

簡易組み立て工程で実現するポリマーエナジーハーベスター

シーズ分野:ものづくり技術, エネルギー

論文

Active microdroplet merging by hydrodynamic flow control using a pneumatic actuator-assisted pillar structure.

Yoon Dong Hyun;Jamshaid Afshan;Ito Junichi;Nakahara Asahi;Tanaka Daiki;Akitsu Takashiro;Sekiguchi Tetsushi;Shoji Shuichi

Lab on a chip査読有り14(16)p.3050 - 30552014年08月-2014年08月 

DOI

詳細

ISSN:1473-0189

概要:This paper describes a microdroplet merging device that can actively control the merging of various droplets under a wide range of flow conditions, using a simple structure. The microdroplets were trapped and merged in a wide chamber divided by pillars, and their behavior was controlled by two horizontal pneumatic microactuators. Hydrodynamic flow control by the actuation was evaluated numerically, and the trapping and merging of droplets were achieved experimentally and controlled via pressure applied to the microactuators. Furthermore, two independently generated droplets were merged under four different modes, ranging from no merging to four-droplet merging, with different ratios and volumes. The pneumatic actuators allowed not only the control of the number of merged droplets, but also a wide range of applied droplet volumes. The device was fabricated simply using a single-layer PDMS (polydimethylsiloxane) structure, and the continuous merging performance operated using only hydrodynamic flow control without any surfactant. Finally, chemical synthesis of a metal complex was performed by the droplet merging method. Crystallization of the complex was visualized in real time, and the synthesis was verified by ultraviolet-visible spectroscopy.

Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Nakamura, Kazuki; Iizuka, Ryo; Nishi, Shinro; Yoshida, Takao; Hatada, Yuji; Takaki, Yoshihiro; Iguchi, Ayaka; Yoon, Dong Hyun; Sekiguchi, Tetsushi; Shoji, Shuichi; Funatsu, Takashi

Scientific Reports査読有り6p.222592016年02月-2016年02月 

DOIScopus

詳細

概要:Environmental microbes are a great source of industrially valuable enzymes with potent and unique catalytic activities. Unfortunately, the majority of microbes remain unculturable and thus are not accessible by culture-based methods. Recently, culture-independent metagenomic approaches have been successfully applied, opening access to untapped genetic resources. Here we present a methodological approach for the identification of genes that encode metabolically active enzymes in environmental microbes in a culture-independent manner. Our method is based on activity-based single-cell sequencing, which focuses on microbial cells showing specific enzymatic activities. First, at the single-cell level, environmental microbes were encapsulated in water-in-oil microdroplets with a fluorogenic substrate for the target enzyme to screen for microdroplets that contain microbially active cells. Second, the microbial cells were recovered and subjected to whole genome amplification. Finally, the amplified genomes were sequenced to identify the genes encoding target enzymes. Employing this method, we successfully identified 14 novel β-glucosidase genes from uncultured bacterial cells in marine samples. Our method contributes to the screening and identification of genes encoding industrially valuable enzymes.

Microfluidic Stamping on Sheath Flow

Yoon, Dong Hyun; Tanaka, Daiki; Sekiguchi, Tetsushi; Shoji, Shuichi

Small査読有りp.3224 - 32282016年06月-2016年06月 

PubMedDOIScopus

詳細

ISSN:16136810

概要:A microfluidic stamping method to form functional shapes on a cross section in fiber‐shaped flow is proposed. Microfluidic stamping and overstamping allow various cross sectional shapes on the 3D flow. The shapes can be controlled by a change in combination of structures and fluidic conditions which correspond to stamp type and stamping force.

Simple microfluidic formation of highly heterogeneous microfibers using a combination of sheath units

Yoon, D. H.; Kobayashi, K.; Tanaka, D.; Sekiguchi, T.; Shoji, S.

Lab on a Chip - Miniaturisation for Chemistry and Biology査読有り17(8)p.1481 - 14862017年01月-2017年01月 

DOIScopus

詳細

ISSN:14730197

概要:© The Royal Society of Chemistry.This paper presents the formation of complex cross-sectional microfibers using three-dimensional microfluidic devices. The compartments and shapes of core and shell layers in the microfibers were independently controlled via three-dimensional fluidic channels fabricated by the combination of sheath units. The number of layers is easily expanded by the stacking of these units. Therefore, the highly heterogeneous microfibers of alginate hydrogel are obtained in polydimethylsiloxane structures. This widely expandable method has great potential for the development of functional and complex fiber-shaped materials.

Construction of integrated gene logic-chip

Takeya Masubuchi, Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama, Yoshie Harada, Takuya Ueda & Hisashi Tadakuma

Nature Nanotechnology査読有り2018年07月-2018年07月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

Stochastic expression of lactate dehydrogenase A induces Escherichia coli persister formation

Yamamoto N Isshiki R Kawai Y Tanaka D Sekiguchi T Matsumoto S Tsuneda S

Journal of Bioscience and Bioengineering査読有り126(1)p.30 - 372018年07月-2018年07月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

概要:Bacterial persisters are phenotypic variants that survive the treatment of lethal doses of growth-targeting antibiotics without mutations. Although the mechanism underlying persister formation has been studied for decades, how the persister phenotype is switched on and protects itself from antibiotics has been elusive. In this study, we focused on the lactate dehydrogenase gene (ldhA) that was upregulated in an Escherichia coli persister-enriched population. A survival rate assay using an ldhA-overexpressing strain showed that ldhA expression induced persister formation. To identify ldhA-mediated persister formation at the single-cell level, time-lapse microscopy with a microfluidic device was used. Stochastic ldhA expression was found to induce dormancy and tolerance against high-dose ampicillin treatment (500 μg/ml). To better understand the underlying mechanism, we investigated the relationship between ldhA-mediated persister formation and previously reported persister formation through aerobic metabolism repression. As a result, ldhA expression enhanced the proton motive force (PMF) and ATP synthesis. These findings suggest that ldhA-mediated persister formation pathway is different from previously reported persister formation via repression of aerobic metabolism.

Mechanical Reinforcement of Low-Concentration Alginate Fibers by Microfluidic Embedding of Multiple Cores

Yoon, Dong Hyun; Tanaka, Daiki; Sekiguchi, Tetsushi; Shoji, Shuichi

Macromolecular Materials and Engineering査読有り(303)p.1700516 - 17005212018年01月-2018年01月 

DOIScopus

詳細

ISSN:14387492

概要:© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This paper presents mechanically reinforced low-concentration alginate fibers by embedding inner cores of high-concentration alginate. 3D structures by stacking multiple polydimethylsiloxane (PDMS) layers allow the microfluidic formation and control of the isolated cores in the continuous flow. The alginate hydrogel fibers are simply spun, and the compartments, central core, surrounding cores, and outer shell layer are successfully verified. The results demonstrate the great potential for the development of complex fibrous materials, particularly for biological applications, which require specific morphology and composition of the fibers.

Synthesis of an azo-Mn(II) complex with mild pH control using a microfluidic device

Daiki Tanaka,*a Shunsuke Sawai,a Dong Hyun Yoon,a Tetsushi Sekiguchi,a Takashiro Akitsub and Shuichi Shoji

RSC Adv.査読有り(7)p.39576 - 395822017年08月-2017年08月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

概要:This study describes the synthesis of an azo-Mn(II) complex requiring an accurate pH control. The reaction conditions for the delicate azo-Mn(II) complex could be precisely controlled using a microfluidic device. The microfluidic method showed the following advantages over the conventional method: the concentration of the pH-control reagent was reduced (1/15), the reaction time was remarkably decreased from 4 h to less than 1 s, and the reaction temperature was lowered from 40 to 23 °C. Moreover, the microfluidic device suppressed the oxidation of the compound and did not require cooling to remove the heat of reaction. In addition, the conventional method uses harsh pH control, whereas the microfluidic device permits mild pH control.

A high-resolution passive droplet-phase sample sorter using multi-stage droplet transfer

Yoon D Xie Z Tanaka D Sekiguchi T Shoji S

RSC Adv.査読有り(7)p.36750 - 367542017年07月-2017年07月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

概要:A fully passive volume-dependent droplet sorter is presented in this research. Repeated and multiple on-rail transfer of microdroplets in a cascade channel resulted in high resolution sorting of droplet-phase samples. Microdroplets with volumes of 0.89–3.26 nl were successfully sorted in eight steps, and a maximum sorting resolution of about 0.21 nl was obtained over three transfer stages. Line rails and dot rails employed in the cascading channels allowed volume-dependent droplet transfer, and shape recovery of the transferred droplet for the repeatable transfer, respectively. This simple device and method can be applied to droplet-based chemical or biological research.

Analysis of vinylidene fluoride–trifluoroethylene copolymer film for nonpolarized energy harvester

Kamata Y Sekiguchi T Nakajima T Sasaki T Dong-Hyun Y Tanaka D Shoji S

Japanese Journal of Applied Physics査読有り56(7)p.070301-1 - 070301-32017年06月-2017年06月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

概要:In this paper, an investigation of a vinylidene fluoride–trifluoroethylene (VDF/TrFE) copolymer film for use in a nonpolarized energy harvester is presented. The Fourier transform infrared spectroscopy and X-ray diffraction analysis of a nonpolarized VDF/TrFE copolymer film indicate that polymer crystallinity depends on film thickness. Furthermore, the growth of the polar structure (β phase) is observed upon the reduction in the thickness. However, power generation does not exhibit a linear behavior because the generation capacity increases with the thickness. These results indicate that the film thickness for a nonpolarized harvester must be optimized to obtain the maximum output.

二重凹角構造ピラーアレイを応用した超撥水性表面の低コスト量産プロセスの開発

中西 完貴;蓑城 森幸;尹 棟鉉;関口 哲志;庄子 習一

電気学会論文誌. E, センサ・マイクロマシン部門誌査読有り137(6)p.165 - 1682017年06月-2017年06月 

DOICiNii

詳細

ISSN:1341-8939

概要:

A new fabrication process of a superrepellent surface using a doubly reentrant structure pillar array was developed. The proposed process applying a soft-lithography is simple, low-cost, and mass-productive process compared to the conventional process. It enables to fabricate a doubly reentrant structure pillar array on glass, silicon, and PDMS substrates.

Simple microfluidic formation of highly heterogeneous microfibers using a combination of sheath units

D. H. Yoon, K. Kobayashi, D. Tanaka, T. Sekiguchi and S. Shoji

Lab on a Chip査読有り(24)p.1481 - 14862017年05月-2017年05月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

概要:This paper presents the formation of complex cross-sectional microfibers using three-dimensional microfluidic devices. The compartments and shapes of core and shell layers in the microfibers were independently controlled via three-dimensional fluidic channels fabricated by the combination of sheath units. The number of layers is easily expanded by the stacking of these units. Therefore, the highly heterogeneous microfibers of alginate hydrogel are obtained in polydimethylsiloxane structures. This widely expandable method has great potential for the development of functional and complex fiber-shaped materials.

Development of an ex vivo Brain Lactic Acid Monitoring System

Negishi S Kamada S Riichi Kajiwara N Kaneko Y Dong Hyun Yoon N Sekiguchi T Shuichi Shoji M Kudo H Member S

査読有り137(1)p.38 - 432017年02月-2017年02月 

DOI

詳細

掲載種別:研究論文(学術雑誌)

概要:An ex vivo brain lactic acid (LA) monitoring system for investigating brain activity at the organ level was constructed and tested in continuous LA monitoring. Our system consists of a micro-fluidic dual-analyte (LA and glucose) biosensor and a special brain chamber that is suitable for organ level experiments. The biosensor utilizes the redox reactions of osmium-wired horseradish peroxidase (Os-HRP) to detect hydrogen peroxide produced by enzyme reactions. The sensor had sufficient sensitivity (LA: ~ 0.01 mM, glucose: ~ 0.01 mM) and selectivity for assessment of extracellular LA. The ex vivo monitoring of brain LA was performed using the proposed system. As a result, the change in extracellular LA was successfully monitored continuously, and the change in the LA level was considered the result of metabolic activity of the sample. Our system is expected to be useful not only for ex vivo brain monitoring, but also for monitoring other organs.

Fabrication process of fluidic devices for producing fine droplets using a focused ion beam system

Nozaki, Y.; Kanai, T.; Matsuo, A.; Tanaka, D.; Yuito, I.; Takeuchi, T.; Sekiguchi, T.; Shoji, S.

16th International Conference on Nanotechnology - IEEE NANO 2016査読有りp.795 - 7982016年11月-2016年11月 

DOIScopus

詳細

掲載種別:研究論文(国際会議プロシーディングス)

概要:© 2016 IEEE.We conducted an experiment on the fabrication of fluidic devices for producing fine droplets. In our proposed method, multiple channels were fabricated by using a focused ion beam (FIB) system. The minimum feature top width was found to be approximately 56 nm. When both the target width and depth of one channel were set to 500 nm, the resultant top width was 750-780 nm, the bottom width was 250-320 nm, and the depth was 560 nm, almost achieving the target size for the channel depth.

Development of a simple fabrication process for a printable piezoelectric energy harvest device

Kamata, Yuki; Yoon, Donghyun; Sasaki, Toshio; Nozaki, Yoshito; Yamaura, Shinichi; Sekiguchi, Tetsushi; Nakajima, Takashi; Shoji, Syuichi

2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016査読有りp.520 - 5232016年11月-2016年11月 

DOIScopus

詳細

掲載種別:研究論文(国際会議プロシーディングス)

概要:© 2016 IEEE.This paper presents a simple printing process to design a novel piezoelectric energy harvest device made from (VDF/TrFE) copolymers. The fabrication process using metal nano-ink and a household printer dramatically reduced the fabrication complexity. Additionally, this process employed low temperatures and thus the structural damage of the polymers resulting from high temperature annealing could be avoided. An electric power of about 0.06 μJ was generated using this device.

Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution

Song, Yanting; Song, Yanting; Takatsuki, Katsuya; Sekiguchi, Tetsushi; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto

Amino Acids査読有り48(7)p.1731 - 17352016年07月-2016年07月 

DOIScopus

詳細

掲載種別:研究論文(学術雑誌)ISSN:09394451

概要:© 2016, Springer-Verlag Wien.This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.

Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

SONG Yanting;TAKATSUKI Katsuya;SEKIGUCHI Tetsushi;FUNATSU Takashi;SHOJI Shuichi;TSUNODA Makoto

CHROMATOGRAPHY査読有り37(3)p.111 - 1162016年07月-2016年07月 

DOICiNii

詳細

概要:Previously, we have developed a gradient elution system for pillar array columns, which achieved faster separation than isocratic elution. In this study, we validated gradient elution in microchip liquid chromatography (LC) and investigated the retention and bandwidth predictions of fluorescently labeled aliphatic amines in fast gradient elution chromatography using semi-empirical retention models. The retention times and peak widths under different gradient elution programs were predicted by three solvent strength models and compared with the experimental results. The relative errors of prediction for the retention times and peak widths were below 14 % and 12 %, respectively. The results showed that the solvent strength models could be utilized for predicting the retention times and peak widths under gradient elution in the microchip LC system and that the gradient elution program for pillar array columns worked efficiently. The prediction by the retention model promises to be a potential tool for essential compound identification in biological samples

Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array

Isokawa M Takatsuki K Song Y Shih K Nakanishi K Xie Z Yoon D Sekiguchi T Funatsu T Shoji S Tsunoda M

Analytical Chemistry査読有り88(12)p.6485 - 64912016年06月-2016年06月 

DOI

詳細

概要:To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This “pillar-distribution-controlled” (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.

Microfluidic synthesis of chiral salen Mn(II) and Co(II) complexes containing lysozyme

Tanaka D Kawakubo W Tsuda E Mitsumoto Y Yoon D Sekiguchi T Akitsu T Shoji S

RSC Advances査読有り(85)p.81862 - 818682016年06月-2016年06月 

DOI

詳細

概要:Efficient microfluidic synthesis of chiral salen Mn(II) and Co(II) complexes containing lysozyme was achieved. The reaction conditions for the delicate protein were controlled precisely with a separation barrier in the microfluidic device. The microfluidic method has the following advantages over the conventional method: a remarkable reduction in the reaction time from 4.5 h to less than 1 s for the synthesis of Mn(II) and Co(II) complexes, a reduction in the reaction temperature from 40 to 23 °C, and no need for an N2 atmosphere because all the reagents are isolated from the air. A three-fold yield improvement was achieved for Mn(II) and Co(II) complexes containing lysozyme compared with conventional synthesis.

Microfluidic Stamping on Sheath Flow

Yoon D Tanaka D Sekiguchi T Shoji S

small査読有り(24)p.1 - 52016年06月-2016年06月 

DOI

詳細

概要:A microfluidic stamping method to form functional shapes on a cross section in fibre-shaped flow is presented by D. H. Yoon and co-workers on page 3224. Microfluidic stamping and overstamping allowed various cross sectional shapes on the three-dimensional flow. Dimension of the flows is controlled via a change in combination of 3D structures and fluidic conditions, which correspond to stamp type and stamping force.

3D Structures: Microfluidic Stamping on Sheath Flow (Small 24/2016)

Yoon, Dong Hyun; Tanaka, Daiki; Sekiguchi, Tetsushi; Shoji, Shuichi

Small2016年06月-2016年06月 

PubMedDOIScopus

詳細

ISSN:16136810

Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array

Isokawa, Muneki; Takatsuki, Katsuya; Song, Yanting; Shih, Kailing; Nakanishi, Kanki; Xie, Zhimin; Yoon, Dong Hyun; Sekiguchi, Tetsushi; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto

Analytical Chemistry査読有り88(12)p.6485 - 64912016年06月-2016年06月 

DOIScopus

詳細

ISSN:00032700

概要:© 2016 American Chemical Society.To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm2 silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.

Culture-independent method for identification of microbial enzyme- encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Nakamura K Iizuka R Nishi S Yoshida T Hatada Y Takaki Y Iguchi A Yoon D Sekiguchi T Shoji S Funatsu T

Scientific Reports査読有り(6)p.222592016年02月-2016年02月 

DOI

詳細

概要:Environmental microbes are a great source of industrially valuable enzymes with potent and unique catalytic activities. Unfortunately, the majority of microbes remain unculturable and thus are not accessible by culture-based methods. Recently, culture-independent metagenomic approaches have been successfully applied, opening access to untapped genetic resources. Here we present a methodological approach for the identification of genes that encode metabolically active enzymes in environmental microbes in a culture-independent manner. Our method is based on activity-based single-cell sequencing, which focuses on microbial cells showing specific enzymatic activities. First, at the single-cell level, environmental microbes were encapsulated in water-in-oil microdroplets with a fluorogenic substrate for the target enzyme to screen for microdroplets that contain microbially active cells. Second, the microbial cells were recovered and subjected to whole genome amplification. Finally, the amplified genomes were sequenced to identify the genes encoding target enzymes. Employing this method, we successfully identified 14 novel β-glucosidase genes from uncultured bacterial cells in marine samples. Our method contributes to the screening and identification of genes encoding industrially valuable enzymes.

All-round micro sheath flow formation to realize complex cross sections by simply stacked PDMs structures

Yoon, Dong Hyun; Ariyoshi, Lisa; Tanaka, Daiki; Sekiguchi, Tetsushi; Shoji, Shuichi

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)2016-Februaryp.141 - 1442016年02月-2016年02月 

DOIScopus

詳細

ISSN:10846999

概要:© 2016 IEEE.This research proposed a sheath flow formation method for complex 3D structure such as multi-core and multi-sheath using multi-stacked PDMS units. Cross sectional shape of the flow and the number of sheath layer were defined simply by combination of different PDMS units. The stacked PDMS units allowed independent fluid injection of different layers and the short sheath area realized low diffusion between each layer. Furthermore, the proposed 3D device fabrication method requires only one point alignment. The sheath flow formation method is useful for wide applications which require fiber materials of specific cross sectional shapes and layers.

Microfluidic synthesis of chiral salen Mn(ii) and Co(ii) complexes containing lysozyme

Tanaka, Daiki; Kawakubo, Wataru; Tsuda, Erika; Mitsumoto, Yuya; Yoon, Dong Hyun; Sekiguchi, Tetsushi; Akitsu, Takashiro; Shoji, Shuichi

RSC Advances査読有り6(85)p.81862 - 818682016年01月-2016年01月 

DOIScopus

詳細

概要:© 2016 The Royal Society of Chemistry.Efficient microfluidic synthesis of chiral salen Mn(ii) and Co(ii) complexes containing lysozyme was achieved. The reaction conditions for the delicate protein were controlled precisely with a separation barrier in the microfluidic device. The microfluidic method has the following advantages over the conventional method: a remarkable reduction in the reaction time from 4.5 h to less than 1 s for the synthesis of Mn(ii) and Co(ii) complexes, a reduction in the reaction temperature from 40 to 23 °C, and no need for an N2 atmosphere because all the reagents are isolated from the air. A three-fold yield improvement was achieved for Mn(ii) and Co(ii) complexes containing lysozyme compared with conventional synthesis.

Stacked piezoelectric energy harvesting device by printing process

Kamata, Yuki;Yoon, Dong Hyun;Sasaki, Toshio;Nozaki, Yoshito;Yamaura, Shinichi;Sekiguchi, Tetsushi;Nakajima, Takashi;Shoji, Shuichi

MICRO & NANO LETTERS11(10)p.650 - 6532016年-2016年

DOIWoS

詳細

ISSN:1750-0443

Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

SONG Yanting;TAKATSUKI Katsuya;SEKIGUCHI Tetsushi;FUNATSU Takashi;SHOJI Shuichi;TSUNODA Makoto

CHROMATOGRAPHY0(0)p.111 - 1162016年-2016年

CiNii

詳細

ISSN:1342-8284

概要:Previously, we have developed a gradient elution system for pillar array columns, which achieved faster separation than isocratic elution. In this study, we validated gradient elution in microchip liquid chromatography (LC) and investigated the retention and bandwidth predictions of fluorescently labeled aliphatic amines in fast gradient elution chromatography using semi-empirical retention models. The retention times and peak widths under different gradient elution programs were predicted by three solvent strength models and compared with the experimental results. The relative errors of prediction for the retention times and peak widths were below 14 % and 12 %, respectively. The results showed that the solvent strength models could be utilized for predicting the retention times and peak widths under gradient elution in the microchip LC system and that the gradient elution program for pillar array columns worked efficiently. The prediction by the retention model promises to be a potential tool for essential compound identification in biological samples.

High efficient synthesis of manganese(II), cobalt(II) complexes containing lysozyme using reaction area separated micro fluidic device

D. Tanaka, Y. Murakoshi, E. Tsuda, Y. Mitsumoto, D. H. Yoon, T. Sekiguchi, T. Akitsu, S. Shoji

18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015査読有りp.243 - 2462015年06月-2015年06月 

DOI

Selective droplet sampling using a minimum number of horizontal pneumatic actuators in a high aspect ratio and highly flexible PDMS device

Dong Hyun Yoon,*a Daisuke Wakui,a Asahi Nakahara,a Tetsushi Sekiguchib and Shuichi Shojia

RSC Advances査読有り(3)p.2070 - 20742015年05月-2015年05月 

DOI

詳細

概要:This paper presents a droplet sampling device driven by horizontal pneumatic actuators. A high aspect ratio and highly flexible PDMS (polydimethylsiloxane) structure was proposed for carrying out larger number of sampling than the number of actuators. Large deformation of the actuators caused domino-deformation of parallel walls, and the deformations allowed for selective collection of target droplets. The dimensions of the PDMS structure and the ratio of resin to curing agent were optimized for efficient sampling under the low pressure applied to the actuators. Five sampling modes were achieved in the simple one-layer structure consisting of one inlet, four walls, one drain channel, and two pneumatic actuators, formed by single-step soft lithography process.

Formation of Polymeric Hollow Microcapsules and Microlenses Using Gas-in-Organic-in-Water Droplets

Yoon, Dong Hyun;Hasegawa, Kenta;Kaneko, Yuji;Arakawa, Takahiro;Go, Jeung Sang;Sekiguchi, Tetsushi;Shoji, Shuichi

MICROMACHINES査読有り6(5)p.622 - 6332015年05月-2015年05月 

DOIWoS

詳細

ISSN:2072-666X

概要:This paper presents methods for the formation of hollow microcapsules and microlenses using multiphase microdroplets. Microdroplets, which consist of a gas core and an organic phase shell, were generated at a single junction on a silicon device without surface treatment of the fluidic channels. Droplet, core and shell dimensions were controlled by varying the flow rates of each phase. When the organic solvent was released from the organic phase shell, the environmental conditions changed the shape of the solidified polymer shell to either a hollow capsule or a microlens. A uniform solvent release process produced polymeric capsules with nanoliter gas core volumes and a membrane thickness of approximately 3 μm. Alternatively physical rearrangement of the core and shell allowed for the formation of polymeric microlenses. On-demand formation of the polymer lenses in wells and through-holes polydimethylsiloxane (PDMS) structures was achieved. Optical properties of the lenses were controlled by changing the dimension of these structures.

Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes

Hosokawa, Masahito; Hosokawa, Masahito; Hosokawa, Masahito; Hoshino, Yuri; Nishikawa, Yohei; Hirose, Tomotada; Yoon, Dong Hyun; Mori, Tetsushi; Mori, Tetsushi; Mori, Tetsushi; Sekiguchi, Tetsushi; Shoji, Shuichi; Shoji, Shuichi; Takeyama, Haruko; Takeyama, Haruko; Takeyama, Haruko

Biosensors and Bioelectronics査読有り67p.379 - 3852015年05月-2015年05月 

PubMedDOIScopus

詳細

ISSN:09565663

概要:© 2014 Elsevier B.V. This paper proposes a high-throughput, function-based screening approach of a metagenomic library for isolating novel microbial enzymes by droplet-based microfluidics. We used gel microdroplets (GMDs) dispersed in oil as picoliter-volume reaction vessels for lipolytic enzyme by encapsulating cells in individual GMDs. Using this approach, we monitored the growth of individual cells encapsulated in GMDs and assessed the enzyme reaction activities at the level of an individual GMD. We then applied this method to screen lipolytic enzyme genes from the metagenomic library constructed from soil collected from a quercus serrate forest of Mount Tsukuba, Ibaraki, Japan. In the workflow presented in this study, metagenomic library clones were encapsulated in 100-pL GMDs with a fluorogenic reporter substrate. A total of 67,000 metagenomic library clones can be screened in only 24. h with reduced consumption of reagents (i.e., <10. μL). As a result, we identified a novel lipolytic enzyme, EstT1, belonging to the EstD2 family of esterases and containing a putative signal peptide, which facilitates enzyme export and catalyzation of substrates in the periplasm. Our study demonstrates the potential of microfluidic GMDs as an efficient tool for metagenomic library screening of industrially relevant enzymes with the potential of significantly reducing the cost and time factors involved in successful practical application of microbial enzymes.

Formation of Polymeric Hollow Microcapsules and Microlenses Using Gas-in-Organic-in-Water Droplets

Dong Hyun Yoon 1,* , Kenta Hasegawa 1, Yuji Kaneko 1, Takahiro Arakawa 2, Jeung Sang Go 3, Tetsushi Sekiguchi 4 and Shuichi Shoji 1

Micromachines査読有り6(5)p.622 - 6332015年04月-2015年04月 

DOI

詳細

概要:This paper presents methods for the formation of hollow microcapsules and microlenses using multiphase microdroplets. Microdroplets, which consist of a gas core and an organic phase shell, were generated at a single junction on a silicon device without surface treatment of the fluidic channels. Droplet, core and shell dimensions were controlled by varying the flow rates of each phase. When the organic solvent was released from the organic phase shell, the environmental conditions changed the shape of the solidified polymer shell to either a hollow capsule or a microlens. A uniform solvent release process produced polymeric capsules with nanoliter gas core volumes and a membrane thickness of approximately 3 μm. Alternatively physical rearrangement of the core and shell allowed for the formation of polymeric microlenses. On-demand formation of the polymer lenses in wells and through-holes polydimethylsiloxane (PDMS) structures was achieved. Optical properties of the lenses were controlled by changing the dimension of these structures.

Highly controllable three-dimensional sheath flow device for fabrication of artificial capillary vessels

J. Ito ; R. Sekine ; D.H. Yoon ; Y. Nakamura ; H. Oku ; H. Nansai ; T. Chikasawa ; T. Goto ; T. Sekiguchi ; N. Takeda ; S. Shoji

Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on査読有り2015年03月-2015年03月 

DOI

詳細

掲載種別:研究論文(国際会議プロシーディングス)

Chemical Reaction in Microdroplets with Different Sizes Containing CdSe/ZnS Quantum Dot and Organic Dye

Takeshi FUKUDA, Tomokazu KURABAYASHI, Hikari UDAKA, Nayuta FUNAKI, Miho SUZUKI, Donghyun YOON, Asahi NAKAHARA, Tetsushi SEKIGUCHI, Shuichi SHOJI

IEICE Transactions on Electronics査読有り(E98.C)p.123 - 1262015年02月-2015年02月 

DOI

詳細

概要:We report a real time method to monitor the chemical reaction in microdroplets, which contain an organic dye, 5(6)-carboxynaphthofluorescein and a CdSe/ZnS quantum dot using fluorescence spectra. Especially, the relationship between the droplet size and the reaction rate of the two reagents was investigated by changing an injection speed.

One Core-Five Sheaths Coaxial Flow Formation Using Multilayer Stacked Flow Focusing Structure

D. H. Yoon, J. Ito, N. Takeda, T. Sekiguchi, S. Shoji

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)査読有りp.539 - 5422015年02月-2015年02月 

DOI

詳細

掲載種別:研究論文(国際会議プロシーディングス)

概要:This research presents efficient coaxial sheath flow formation using a three-dimensional PDMS device. In the device, one core and five sheaths are simply formed with low diffusion between different samples. Stacking and alignment of six PDMS layers of same structure allowed for fabrication of the proposed 3D device. Only one point alignment of center channel for the sheath provides free from misalignment that was a critical problem of multi-stacked PDMS device. Furthermore, the number of samples is infinitely expandable by increase in the number of stacking layers. The coaxial sheath flow is useful for biological fiber formation which requires multilayer of different materials such as artificial blood vessels or muscles.

Chemical Reaction in Microdroplets with Different Sizes Containing CdSe/ZnS Quantum Dot and Organic Dye

FUKUDA Takeshi;KURABAYASHI Tomokazu;UDAKA Hikari;FUNAKI Nayuta;SUZUKI Miho;YOON Donghyun;NAKAHARA Asahi;SEKIGUCHI Tetsushi;SHOJI Shuichi

IEICE Transactions on Electronics査読有りE98.C(2)p.123 - 1262015年02月-2015年02月 

DOICiNii

詳細

ISSN:0916-8524

概要:We report a real time method to monitor the chemical reaction in microdroplets, which contain an organic dye, 5(6)-carboxynaphthofluorescein and a CdSe/ZnS quantum dot using fluorescence spectra. Especially, the relationship between the droplet size and the reaction rate of the two reagents was investigated by changing an injection speed.

Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes

Hosokawa, Masahito;Hoshino, Yuri;Nishikawa, Yohei;Hirose, Tomotada;Yoon, Dong Hyun;Mori, Tetsushi;Sekiguchi, Tetsushi;Shoji, Shuichi;Takeyama, Haruko

BIOSENSORS & BIOELECTRONICS67p.379 - 3852015年-2015年

DOIWoS

詳細

ISSN:0956-5663

環境・エネルギー材料開発分野の活動状況

山浦 真一;関口 哲志;余語 利信

まてりあ54(5)p.227 - 2312015年-2015年

CiNii

詳細

ISSN:1340-2625

Selective droplet sampling using a minimum number of horizontal pneumatic actuators in a high aspect ratio and highly flexible PDMS device

Yoon, Dong Hyun;Wakui, Daisuke;Nakahara, Asahi;Sekiguchi, Tetsushi;Shoji, Shuichi

RSC ADVANCES査読有り5(3)p.2070 - 20742014年12月-2014年12月 

DOIWoS

詳細

ISSN:2046-2069

概要:This paper presents a droplet sampling device driven by horizontal pneumatic actuators. A high aspect ratio and highly flexible PDMS (polydimethylsiloxane) structure was proposed for carrying out larger number of sampling than the number of actuators. Large deformation of the actuators caused domino-deformation of parallel walls, and the deformations allowed for selective collection of target droplets. The dimensions of the PDMS structure and the ratio of resin to curing agent were optimized for efficient sampling under the low pressure applied to the actuators. Five sampling modes were achieved in the simple one-layer structure consisting of one inlet, four walls, one drain channel, and two pneumatic actuators, formed by single-step soft lithography process.

Development of an electrohydrodynamic ion-drag micropump using three-dimensional carbon micromesh electrodes

Yoon, Dong Hyun;Sato, Hironobu;Nakahara, Asahi;Sekiguchi, Tetsushi;Konishi, Satoshi;Shoji, Shuichi

JOURNAL OF MICROMECHANICS AND MICROENGINEERING査読有り24(9)p.0950032014年07月-2014年07月 

DOIWoS

詳細

ISSN:0960-1317

概要:An electrohydrodynamic (EHD) ion-drag micropump using three-dimensional carbon micromesh electrodes was developed. The carbon micromesh electrodes were created by the pyrolysis of SU-8 structures. The carbon electrodes and microchannel were formed on a quartz substrate, and the microchannel was sealed by an SU-8 slab structure. The pumping behaviors were evaluated using Fluorinert as a non-conductive sample solution. The maximum pressure and volume flow rate were approximately 23 Pa and 400 nL/min, respectively, under an applied voltage of 500 V.

Development of electrohydrodynamic ion-drag micropump using three-dimensional carbon micromesh electrodes

Yoon, Dong Hyun; Sato, Hironobu; Nakahara, Asahi; Sekiguchi, Tetsushi; Konishi, Satoshi; Shoji, Shuichi

Journal of Micromechanics and Microengineering2014年06月-

Active microdroplet merging by hydrodynamic flow control using pneumatic actuator-assisted pillar structure

Dong Hyun Yoon, Afshan Jamshaid, Junichi Ito, Asahi Nakahara, Daiki Tanaka, Takashiro Akitsu, Tetsushi Sekiguchi and Shuichi Shoji

Lab on a Chip2014年06月-

DOI

Active microdroplet merging by hydrodynamic flow control using a pneumatic actuator-assisted pillar structure

Yoon, Dong Hyun;Jamshaid, Afshan;Ito, Junichi;Nakahara, Asahi;Tanaka, Daiki;Akitsu, Takashiro;Sekiguchi, Tetsushi;Shoji, Shuichi

LAB ON A CHIP査読有り14(16)p.3050 - 30552014年06月-2014年06月 

DOIWoS

詳細

掲載種別:研究論文(学術雑誌)ISSN:1473-0197

概要:This paper describes a microdroplet merging device that can actively control the merging of various droplets under a wide range of flow conditions, using a simple structure. The microdroplets were trapped and merged in a wide chamber divided by pillars, and their behavior was controlled by two horizontal pneumatic microactuators. Hydrodynamic flow control by the actuation was evaluated numerically, and the trapping and merging of droplets were achieved experimentally and controlled via pressure applied to the microactuators. Furthermore, two independently generated droplets were merged under four different modes, ranging from no merging to four-droplet merging, with different ratios and volumes. The pneumatic actuators allowed not only the control of the number of merged droplets, but also a wide range of applied droplet volumes. The device was fabricated simply using a single-layer PDMS (polydimethylsiloxane) structure, and the continuous merging performance operated using only hydrodynamic flow control without any surfactant. Finally, chemical synthesis of a metal complex was performed by the droplet merging method. Crystallization of the complex was visualized in real time, and the synthesis was verified by ultraviolet-visible spectroscopy.

Effect of Annealing on Magnetostrictive Properties of Fe­Co Alloy Thin Films

Nakajima T Takeuchi T Yuito I Kato K Saito M Abe K Sasaki T Sekiguchi T Yamaura S

MATERIALS TRANSACTIONS査読有り55(3)p.556 - 5602014年03月-2014年03月 

DOI

詳細

概要:The effect of the annealing temperature on the magnetostrictive properties of Fe–Co alloy thin films on quartz glass was systematically investigated. The saturation magnetostriction was 56 ppm for the Fe32Co68-sputtered thin film quenched from 673 K, and it increased to 159 ppm with increasing annealing temperature up to 1073 K. The magnetostriction above 1093 K significantly decreased with the emerging fcc phase in the Fe–Co alloy. SEM images showed that the crystal grains present in the fcc phase aggregated to form a discontinuous surface, which resulted in a decrease in the effective magnetostriction. Measurements of the magnetic properties by vibrating sample magnetometer and magnetic force microscopy revealed the enhancement of the magnetization at the crystal grain boundaries of Fe–Co alloy thin films with large magnetostriction. Thus, the heterogeneity of the magnetization can play a key role in inducing the large magnetostrictive effect.

Effect of Annealing on Magnetostrictive Properties of Fe-Co Alloy Thin Films

Nakajima, Takashi;Takeuchi, Teruaki;Yuito, Isamu;Kato, Kunio;Saito, Mikiko;Abe, Katsuhiro;Sasaki, Toshio;Sekiguchi, Tetsushi;Yamaura, Shin-ichi

MATERIALS TRANSACTIONS査読有り55(3)p.556 - 5602014年03月-2014年03月 

DOIWoS

詳細

掲載種別:研究論文(学術雑誌)ISSN:1345-9678

概要:Abstract The effect of the annealing temperature on the magnetostrictive properties of Fe–Co alloy thin films on quartz glass was systematically investigated. The saturation magnetostriction was 56 ppm for the Fe32Co68-sputtered thin film quenched from 673 K, and it increased to 159 ppm with increasing annealing temperature up to 1073 K. The magnetostriction above 1093 K significantly decreased with the emerging fcc phase in the Fe–Co alloy. SEM images showed that the crystal grains present in the fcc phase aggregated to form a discontinuous surface, which resulted in a decrease in the effective magnetostriction. Measurements of the magnetic properties by vibrating sample magnetometer and magnetic force microscopy revealed the enhancement of the magnetization at the crystal grain boundaries of Fe–Co alloy thin films with large magnetostriction. Thus, the heterogeneity of the magnetization can play a key role in inducing the large magnetostrictive effect.

Real-time monitoring of chemical reaction in microdroplet using fluorescence spectroscopy

Fukuda, Takeshi; Funaki, Nayuta; Kurabayashi, Tomokazu; Suzuki, Miho; Yoon, Dong Hyun; Nakahara, Asahi; Sekiguchi, Tetsushi; Shoji, Shuichi

Sensors and Actuators, B: Chemical査読有り203p.536 - 5422014年01月-2014年01月 

DOIScopus

詳細

ISSN:09254005

概要:We report a novel real-time method to monitor the reaction of an organic dye, 5(6)-carboxynaphthofluorescein succinimidyl ester and a CdSe/ZnS quantum dot (QD) using fluorescence spectra of microdroplets formed in a microfluidic device. Dual fluorescence peaks (at λ = 605 and 670 nm) were observed via a fluorescence energy transfer (FRET) process from the CdSe/ZnS QD to the organic dye; the rate of the chemical reaction of the two reagents was estimated by the ratio of the two fluorescence peaks, defined as the fluorescence intensity ratio. In addition, well-controlled microdroplets were continuously generated (with a diameter of ∼150 μm) in the microfluidic device and the reaction time was determined as the length from the mixing point to the sampling point using fluorescence spectroscopy. The fluorescence intensity ratio increased with increasing reaction time after mixing of the two reagents indicating that the chemical reaction was observed directly in the microdroplet. Therefore, the real-time monitoring of the chemical reaction was successfully achieved by measuring the fluorescence intensity ratio as a function of the reaction time. When compared with a conventional batch process, a fast reaction time of <1 s and a high fluorescence intensity ratio was achieved in the microdroplets formed in the microfluidic device. © 2014 Elsevier B.V.

Hydrodynamic on-rail droplet pass filter for fully passive sorting of droplet-phase samples

Yoon, Dong Hyun;Numakunai, Satoshi;Nakahara, Asahi;Sekiguchi, Tetsushi;Shoji, Shuichi

RSC ADVANCES4(71)p.37721 - 377252014年-2014年

DOIWoS

詳細

ISSN:2046-2069

Real-time monitoring of chemical reaction in microdroplet using fluorescence spectroscopy

Fukuda, Takeshi;Funaki, Nayuta;Kurabayashi, Tomokazu;Suzuki, Miho;Yoon, Dong Hyun;Nakahara, Asahi;Sekiguchi, Tetsushi;Shoji, Shuichi

SENSORS AND ACTUATORS B-CHEMICAL203p.536 - 5422014年-2014年

DOIWoS

詳細

ISSN:0925-4005

Controllable active micro droplets merging device using horizontal pneumatic micro valves

Jamshaid, Afshan; Igaki, Masaya; Yoon, Dong Hyun; Sekiguchi, Tetsushi; Shoji, Shuichi

Micromachines4(1)p.34 - 482013年10月-2013年10月 

DOIScopus

詳細

概要:We present an active droplet merging device, which can merge various sizes of micro droplets in different numbers by using pneumatically controlled horizontal PDMS microvalves. The merging part consists of a main and side channels separated by a pillar array. The pillar array structure is contained within a microfuidic channel. The function of the pillar array provides a bypass path to the continuous flow (oil) inside the merging chamber. Droplets are successfully generated within the channel and achieve merging by controlling the selective different numbers and diameters of droplets through varying the flow resistance of main and side channel. In the merging chamber, a droplet will enter and slow down its movement. It will wait and then merge with the sequential droplets. These experiments demonstrate that such a merging device can controllably select and adjust the distance between the different adjacent micro droplets without any generation of sister droplets in the side channel. The device has no desynchronization problems. Thus, it can be applied for efficiently mixing the droplets in various diameters and numbers without changing the structure of the merging chamber. Hence, this device can be a more effective choice when applying microfluidics to chemical and biological applications.

Local cell damage-induced apoptosis analysis on microfluidic system

Kajiyama, Risa;Watanabe, Taku;Edagawa, Yoshikuni;Sekiguchi, Tetsushi;Shoji, Shuichi

JOURNAL OF PHARMACOLOGICAL SCIENCES121p.220P - 220P2013年-2013年

WoS

詳細

ISSN:1347-8613

Fast and quantitative analysis of branched-chain amino acids in biological samples using a pillar array column

Song, Yanting;Takatsuki, Katsuya;Isokawa, Muneki;Sekiguchi, Tetsushi;Mizuno, Jun;Funatsu, Takashi;Shoji, Shuichi;Tsunoda, Makoto

ANALYTICAL AND BIOANALYTICAL CHEMISTRY405(25)p.7993 - 79992013年-2013年

DOIWoS

詳細

ISSN:1618-2642

Active and Precise Control of Microdroplet Division Using Horizontal Pneumatic Valves in Bifurcating Microchannel

Yoon, Dong Hyun;Ito, Junichi;Sekiguchi, Tetsushi;Shoji, Shuichi

MICROMACHINES4(2)p.197 - 2052013年-2013年

DOIWoS

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ISSN:2072-666X

Effect of Hydrogen Absorption on Electrical Transport Properties for Ni36Nb24Zr40 Amorphous Alloy Ribbons

Umetsu, Rie Y.;Yoshida, Hajime;Fukuhara, Mikio;Yamaura, Shin-ichi;Matsuura, Makoto;Sasaki, Toshio;Sekiguchi, Tetsushi;Saito, Mikiko;Mizuno, Jun;Kawarada, Hiroshi

MATERIALS TRANSACTIONS54(8)p.1339 - 13422013年-2013年

DOIWoS

詳細

ISSN:1345-9678

Matrix Arrangement of Three-Dimensional Sheath Flow for Multiple Component Nanofibers

Yoon, Dong Hyun;Nakahara, Asahi;Jamshaid, Afshan;Sato, Hironobu;Sekiguchi, Tetsushi;Shoji, Shuichi

JOURNAL OF SENSORS2013年-2013年

DOIWoS

詳細

ISSN:1687-725X

医療・バイオセンサとネットワークセンシング : 解説特集の企画にあたって

関口 哲志

電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society132(11)2012年11月-2012年11月 

CiNii

詳細

ISSN:13418939

概要:本記事に「抄録」はありません。

Precisely controlled micro droplet merging device using horizontal pneumatic valves

M. Igaki, D. H. Yoon, T. Sekiguchi, and S. Shoji

IEEE-NEMS 20122012年03月-

Continuous size-selective separation using three dimensional flow realized by multilayer PDMS structure

D. H. Yoon, T. Sekiguchi, and S. Shoji

The 25th International Conference on Micro Electro Mechanical Systemsp.1205 - 12082012年01月-

Size-oriented passive droplets sorting by using surface free energy with micro guide groove

Y. Harada, D. H. Yoon, T. Sekiguchi, and S. Shoji

The 25th International Conference on Micro Electro Mechanical Systems: MEMS 2012p.1105 - 11082012年01月-

Integration of Pillar Array Columns into a Gradient Elution System for Pressure-Driven Liquid Chromatography

Song, Yanting;Noguchi, Masao;Takatsuki, Katsuya;Sekiguchi, Tetsushi;Mizuno, Jun;Funatsu, Takashi;Shoji, Shuichi;Tsunoda, Makoto

ANALYTICAL CHEMISTRY84(11)p.4739 - 47452012年-2012年

DOIWoS

詳細

ISSN:0003-2700

Controllable three-dimensional sheath flow with a wide range Reynolds number and its application for efficient cell sorting

R. Sekine, T. Sakurai, D. H. Yoon, R. Iizuka, T. Sekiguchi, T. Funatsu, and S. Shoji

The 15th International Conference of Miniaturized Systems for Chemistry and Life Sciences: µTAS 2011p.1903 - 19052011年10月-

Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

M. Haneoka, Y. Shirasaki, H. Sugino, T. Sekiguchi, D. H. Yoon, R. Iizuka, S. Shoji, and T. Funatsu

The 15th International Conference of Miniaturized Systems for Chemistry and Life Sciences: µTAS 2011p.1173 - 11752011年10月-

Multilayer PDMS (polydimethylsiloxane) device fabrication using concave-convex 3D alignment pair structure

D. H. Yoon, M. Kawai, T. Sekiguchi, and S. Shoji

ISMM 2011 in Conjunction with the KBCS Spring Meetingp.F3 - 0912011年06月-

Three-dimensional sample flow focusing and positioning for wide range flow speed application

R. Sekine, D. H. Yoon, T. Sekiguchi, and S. Shoji

ISMM 2011 in Conjunction with the KBCS Spring Meetingp.T3 - 0222011年06月-

Micro/Nanofluidic devices for chemical/biochemical micro total analysis systems

S. Shoji, K. Kawai, H. Shinohara, D.H. Yoon, J. Mizuno, T. Sekiguchi, T. Arakawa, T. Funatsu

The 2nd NIMS(MANA)−Waseda International Symposiump.OP052010年12月-

Selective droplet sampling flow system using minimum number of horizontal pneumatic valves formed by single step PDMS molding

D. H. Yoon, D. Wakui, T. Sekiguchi, and S. Shoji

The 14th International Conference of Miniaturized Systems for Chemistry and Life Sciences: µTAS 2010p.1085 - 10872010年10月-

MULTI CHANNEL DROPLET SORTING DEVICE WITH HORIZONTAL PNEUMATIC ACTUATION USING SINGLE LAYER PDMS FLEXIBLE PARALLEL WALLS

Daisuke Wakui, Shuhei Takahashi, Tetsushi Sekiguchi, Shuichi Shoji

23nd IEEE International Conference on Micro Electro Mechanical Systems: MEMS 2010p.144 - 1472010年01月-

EHD Micro PumpUsing Pyrolyzed Polymer 3-D Carbon Mesh Electrodes

D. Wakui, N. Imai, Y. Nagaura, H. Sato, T. Sekiguchi, S. Konishi, S. Shoji

22nd IEEE International Conference on Micro Electro Mechanical Systems: MEMS 2009p.499 - 5022009年01月-

COMPARISON OF A MICROFLUIDIC GAS-INTAKE DEVICES WITH PILLAR-TYPE AND MICROFLUIDIC MEMBRANE-TYPE STRUCTURES.

M. Amano, T. Arakawa, H. Sato, T. Sekiguchi, S. Shoji, H. Kudo, H. Saito, K. Mitsubayashi

ECS Transactions16(14)p.117 - 1222008年10月-

Multiple core-sheath liquid transfer using matrix arrangement of 3D sheath flows

H.Sato, Y.Sasamoto, T.Sekiguchi, T.Homma, S.Shoji

International Conference on Miniaturized Systems for Chemistry and Life Sciences (mTAS 2007) Paris, Francep.1571 - 15732007年10月-

3D sheath flow using hydrodynamic position control of the sample flow

H.Sato, Y.Sasamoto, D.Yagyu, T.Sekiguchi, S.Shoji

Journal of Micromechanics and Microengineering17p.2211 - 22162007年-

Flow Type Electrochemical Immuno Sensing Systems

Yutaka Yamaguchi, Nobuo Honda, Masashi Inaba, Tetsushi Sekiguchi and Shuichi Shoji

IEEE SENSOR (2006)p.138 - 1402006年-

臨床現場とメーカをつなぐ臨床工学部門

関口哲志

Clinical Engineering15(7)p.721 - 7222004年07月-

新しい術中モニタリング

関口哲志

Clinical Engineering15(7)2004年-

企業が考える臨床技士教育

関口哲志

Clinical Engineering14(7)p.705 - 7072003年07月-

Particles and molecules handling in micro channels

S.Shoji, K.Tashiro, T.Sekiguchi, H.Sato, N.Honda, T.Funatu,

Lab-on-a-chip, Miniaturies Systems for (Bio)Chemical Analysis and Synthesis, Elsevier B.V., Amsterdamp.205 - 2142003年-

実験的発作性頻拍時の胃粘膜二酸化炭素分圧の連続モニタ

崎尾秀彰, 上野和徳, 関口哲志, 永井裕子

日本救急医学会誌(JJAAM)(14)p.433 - 4392003年-

A Novel Biomolecule Sorter Using Thermosensitive Hydrogel in Micro Flow Systems

Y.Shirasaki, H.Makazu, K.Tashiro, S.Ikeda, T.Sekiguchi, S.Shoji,S.Tsukita, T.Funatsu

Proc. Micro Total Analysis Systems 2002; μTAS2002, Narap.925 - 9272002年-

DOI

Biomolecules Sorting System using Thermal Gelation of aMethyl Cellulose Solution

S.Ikeda, K.Tashiro, T.Sekiguchi, S.Shoji, H.Makazu, Y.Shirasaki, T.Funatsu, S.Tsukita

Dig. Int. Microprocesses and Nanotechnology Conf.; MNC2002, Tokyop.170 - 1712002年-

Micro Flow Devices and Biomoleculer Handling in Micro Flow Channels

S.Shoji, N.Honda, H.Sato, K.Tashiro, T.Sekiguchi, T.Funatsu

Proc. 1st Int. Symp. On Ultramicrochemical Process:ISUP 2002p.83 - 892002年-

A PARTICLES AND BIOMOLECULES SORTING MICRO FLOW SYSTEM USING THERMAL GELATION OF METHYL CELLULOSE SOLUTION

K. TashiroS. IkedaT. SekiguchiS. ShojiH. MakazuT. FunatsuS. Tsukita

Micro Total Analysis Systems 2001査読有り2001年10月-2001年10月 

DOI

詳細

掲載種別:研究論文(国際会議プロシーディングス)

Micro Flow Switches using Thermal Gelation of Methylcellulose for Biomolecules Handling

Koichi Tashiro, Shingo Ikeda, Tetsushi Sekiguchi, Hironobu Sato, Shuichi Shoji, Hiroshi Makazu, Kenjiro,Watanabe, Takashi Funatsu, Shoichiro Tsukita

TRANSDUCERS '01 EUROSENSORS XV. The 11th International Conference on Solid-StateSensors and Actuators, Munich, Germany,p.932 - 9352001年06月-

Continuous measurement of gastric luminal CO2 (PgCO2) and Cardiac Output (CCO) through the perioperative period in the patients receiving surgery using cardiopulmonary bypass (CPB)

Takasuke Imai, Tetsushi Sekiguchi, Yuko Nagai, Takushi Morimoto Toshihisa Nosaka, Chieko Mitaka, Kousi,Makita, Makoto Sunamori

30th international education & scientific symposium on critical care medicine. San Francisco California.USA2001年02月-

電気学会マイクロ化学分析システム調査専門委員会報告書「マイクロ化学分析システム(μ‐TAS)技術動向」

関口哲志他

マイクロ化学分析システム調査専門委員会2000年12月-

臨床工学技士 21世紀の夢 −HELP? HELP!

関口哲志

Clinical Engineering11(11)p.941 - 9442000年11月-

静電駆動を用いた蠕動型マイクロポンプの試作

佐藤寛暢, 関口哲志, 庄子習一

第二回化学とマイクロシステム研究会 姫路2000年09月-

生体用テレメトリ開発における電磁ソフトウエアシミュレータの有用性の検討

石塚洋介, 伊藤雄一, 関口哲志, 庄子習一

第39回日本ME 学会大会 医用電子と生体工学38p.2692000年05月-

開心術における舌下組織PCO2の測定

井上久, 佐藤勲, 仁杉直子, 三宅実砂, 佐藤陽二, 池上敬一, 中川儀英, 永井裕子, 関口哲志

日本集中治療医学会総会p.2152000年03月-

Design and Simulation of Particles and Biomolecules Handling Micro Flow Cells with Tree-dimensional Sheath Flow

Koichi Tashiro, Tetsushi Sekiguchi, Shuichi Shoji, Takashi Funatsu, Hironobu Sato, Wataru Masumoto

Proc. Micro Total Analysis Systemsp.209 - 2122000年-

Detection of perfusion failure during open heart surgery with sublingual PCO2

Hisashi Inoue, Isamu Sato, Naoko Hitosugi, Yoshito Takano, Misa Miyake, Yoji Sato, Keiichi Ikegami, Yuko Nagai, Tetsushi Sekiguchi Michihiro Nakamura

20th International Symposium on Intensive Care and Emergency Medicine4(1)p.1572000年-

Evaluation of PCO2 measurement using a gastric air tonometer located in non-mixing solution.

Yoji Sato, Hisashi Inoue, Keiichi Ikegami, Tomoko Seki, Fumio Morimoto, Yuko Nagai, Tetsushi Sekiguchi,Michihiro Nakamura

20th International Symposium on Intensive Care and Emergency Medicine4(1)p.1512000年-

Immunological Helicobactor pylori urease analyzer based on ion-sensitive field effect transistor

Tetsushi Sekiguchi, Michihiro Nakamura, Mototsugu Kato, Keiko Nishikawa, Kaku Hokari, Toshiro Sugiyama, Masahiro Asaka

Sensors and Actuators B(67)p.265 - 2692000年-

新しい医療用マイクロ化学・生化学分析システム −uTAS−

庄子習一・関口哲志

SUT BULLETIN(5)p.38 - 432000年-

救急現場でのスパゲッティ症候群の解消−新しい心電図検出法(コードレス電極)の開発−

佐藤陽二, 関口哲志

第27回日本救急医学会総会p.1931999年11月-

医用無線通信機器とEMC −病院内複数機器からのエミッションの影響について−

関口哲志, 松村文幸, 坂田博史, 稲井 隆, 原田 元, 保坂栄弘, 佐藤陽二

電気学会全国大会1999年03月-

Immunological Rapid Urease Test for Detecting Helicobactor Pylori: Comparative Study of Biopsy Specimens and Gastric Mucus

Tadashi Sato, Masayuki A. Fujino, Yuichiro Kojima, Fumiaki Kitahara, Toshiya Nakamura, Atsuro Morozumi,Kumiko Nagata,Tetsushi Sekiguchi, Michihiro Nakamura, Hidehiro Hosaka

Dig. Endosc.11(1)p.42 - 461999年-

座談会:臨床工学技士これまでの十年これからの十年

関口哲志

Clinical Engineering10(7)p.666 - 6761999年-

開心術症例の胃腔PCO2連続モニタ

根本真人、高橋宏行、崎尾秀彰、関口哲志、永井裕子

第38回日本麻酔学会関東甲信越大会1998年09月-

近接して複数の医療機器が使用される医療現場で、医療用通信機器が使用される場合の問題点は何か?

関口哲志, 松村文幸, 坂田博史, 稲井 隆, 原田 元, 保坂栄弘

第4回医療電磁環境研究会p.38 - 451998年07月-

医用テレメータ開発における病院内電磁環境の問題点について

関口哲志, 松村文幸, 坂田博史, 稲井 隆, 原田 元, 保坂栄弘

第37回日本エム・イー学会大会p.3641998年05月-

2種類の胃内腔PCO2連続モニタの比較検討

高橋宏行、大津 敏、崎尾秀彰、関口哲志、永井裕子

第13回日本SHOCK学会ポスターセッション1998年04月-

Gastric PCO2 monitoring system based on a double membrane type PCO2 sensor

Tetsushi Sekiguchi, Yuko Nagai, Tetsuya Makino, Kohei Ohno, Michihiro Nakamura, Hidehiro Hosaka,Hideaki Sakio, Satoshi Ohtsu, Hiroyuki Takahashi

Sensors and Actuators B(49)p.171 - 1781998年-

電気学会マイクロ化学センサ調査研究専門委員会報告書「マイクロ化学センサの技術動向」

関口哲志他

マイクロ化学センサ調査専門(委)1998年-1998年

A Very Low-Power Consumption Wireless ECG Monitoring System Using Body as a Signal Transmission Medium

Takashi Handa, Shuichi Shoji, Shinichi Ike, Sunao Takeda, Tetsushi Sekiguchi

International Conference on Solid-State Sensors and Actuators, Chicagop.1003 - 10061997年06月-

胃内腔PCO2連続測定装置の開発

上野和徳、田中禎一、崎尾秀彰、関口哲志、永井裕子、中村通宏

第12回日本SHOCK学会ポスターセッション1997年05月-

生体情報計測用マイクロシステムの研究−心電位計測用マイクロシステム−

半田 崇、庄子習一、武田 朴、関口哲志

電気学会全国大会(3)p.125 - 1261996年-

血圧トランスデューサの性能改善

小沢秀夫、浜谷哲男、武田 朴、兵後充史、関口哲志、江刺正喜

第25回日本ME学会ポスターセッションp.5001986年-

"−Preparation of Polysiloxanes from Silicic Acid. V. − Condensation of Silicic Acid Butyl Esters and Formation of Silica Fiber from the Ester Solutions

Yoshimoto Abe, Tetsushi Sekiguchi, and Takahisa Misono

JOURNAL OF POLYMER SCIENCE:Polymer Chemistry Edition22(3)p.925 - 9271984年03月-

Structural Formation of Oil-in-Water (O/W) and Water-in-Oil-in-Water (W/O/W) Droplets in PDMS Device Using Protrusion Channel without Hydrophilic Surface Treatment

D.H. Yoon, D. Tanaka, T. Sekiguchi, S. Shoji

Micromachines査読有り9(9)p.4682018年08月-2018年08月 

DOI

Integration of Horizontal and Vertical Microfluidic Modules for Core-Shell Droplet Generation and Chemical Application

Yoon D Nozaki Y Tanaka D Sekiguchi T Shoji S

Micromachines査読有り10((9))p.613 - 6242019年09月-

DOI

Simple and Rapid Fabrication Process of Porous Silicon Surface Using Inductively Coupled Plasma Reactive Ion Etching

T. Sugaya, D.H. Yoon, H. Yamazaki, K. Nakanishi, T. Sekiguchi, S. Shoji

Journal of Microelectromechanical Systems査読有り29(1)p.62 - 672019年11月-2019年11月 

DOI

High-Efficiency Dibromination of Organic Compound in Microfluidic Channel of Si Pillar Array Directly Coated with Iron Catalyst

T. Sugaya, D. Tanaka, D.H. Yoon, Y. Nozaki, T. Sekiguchi, T. Akitsu, S. Shoji

The 20th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2019)査読有りp.2278 - 22812019年06月-2019年06月 

DOI

書籍等出版物

Particles and molecules handling in micro channels” Miniaturized Systems for (Bio)Chemical Analysis and Synthesis

Lab-on-a-chip

Elsevier2003年-

臨床現場とメーカをつなぐ臨床工学部門

Clinical Engineering

秀潤社2004年 07月-

新しい術中モニタリング

Clinical Engineering

秀潤社2004年 07月-

企業が考える臨床工学技士教育

Clinical Engineering

秀潤社2003年 07月-

「マイクロ化学分析システム(μ‐TAS)技術動向」

電気学会2000年-

「臨床工学技士 21世紀の夢 」

Clinical Engineering

秀潤社2000年 11月-

「新しい医療用マイクロ化学・生化学分析システム −μTAS−」

東京理科大学出版会2000年-

臨床工学技士これまでの十年これからの十年

Clinical Engineering

秀潤社1999年 10月-

講演・口頭発表等

High Yield Micro Fluidic Synthesis of Metal Complex Containing Proteins

2014年07月30日

詳細

口頭発表(一般)

real time monitoring of chemical reaction in micro droplet with quantum dot and dye using Fluorescence spectroscopy

2014年05月15日

詳細

ポスター発表

Peptide-based ligand screening system for G protein-coupled receptors (GPCRs) using water-in-oil microdroplets

第52回日本生物物理学会年会(BSJ20142014年09月25日

詳細

ポスター発表

Development of fluorescence-activated droplet sorting system using thermoreversible gelation polymer and its application

第52回日本生物物理学会年会2014年09月25日

詳細

ポスター発表

量子ドット‐色素間の反応速度に微小液滴のサイズが与える影響

応用物理学会2014年09月

詳細

口頭発表(一般)

Three-Dimensional Sample Flow Focusing for Wide Range Flow Speed Applications

2011年06月23日

詳細

ポスター発表

3次元カーボンメッシュ電極を用いたEHDマイクロポンプ

2009年01月

詳細

ポスター発表

マイクロガス取り込みデバイスの比較

日米電気化学合同会議2008年10月

詳細

ポスター発表

CRYSTALLIZATION OF ZINC(II) COMPLEX CONTAINING LYSOZYME BY SUPER WATER REPELLENT DOUBLY REENTRANT STRUCTURE UMBRELLA PILLAR ARRAY

Tanaka D Kawakubo W Yoon D Sekiguchi T Akitsu T Shoji S

THE 3RD CONFERENCE ON MICROFLUIDIC HANDLING SYSTEMS, 4-6 OCTOBER 20172017年10月

詳細

国際会議口頭発表(一般)

概要: This paper reports the crystallization of a functional protein in the droplet on a super water repellent structure that consists of an umbrella-like pillar array. The structure provided certain participation of small volume reagents in the crystallization reaction. The crystallization conditions of functional protein were performed under room temperature and the atmosphere. Furthermore, the open structure allowed simple picking fragile crystals up. The umbrella-like pillar array structure was fabricated by soft MEMS process using SU-8 and polydimethylsiloxane (PDMS) simply. This research proposes a new crystallization technology for functional proteins, and the result of this research is useful for the analysis of functional proteins in the future.

Extremely Efficient and Non-Hazardous Bromo Group Addition Reaction Using Simple Microfluidic Devices

W. Kawakubo, D. Tanaka, D.H. Yoon, T. Sekiguchi, K. Takahashi, T. Akitsu, S. Shoji

The 3rd Conference on Microfluidic Handling Systems (MFHS 2017)2017年10月

詳細

国際会議ポスター発表

A Micromachined Liquid Chromatography Chip with a Pillar Array Mixer for Post-Column Derivatization in the Analysis of Neurotransmitters

M. Isokawa, K. Nakanishi, T. Kanamori, H. Zhuang, H. Yamazaki, T. Sugaya, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda

The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2017)2017年10月

詳細

国際会議ポスター発表

Simple Formation of Complex Cross-Sectional Flow Using Multi-Stacked Three-Dimensional PDMS Structures

D.H. Yoon, T. Sekiguchi, S. Shoji

CIAiS (Research and Education Consortium for Innovation of Advanced Integrated Science) International Symposium 20182018年03月

詳細

国際会議ポスター発表

Crystallization of Zinc(II) Complex Containing Lysozyme by Super Water Repellent Umbrella Pillar Array

D. Tanaka, W. Kawakubo, D.H. Yoon, T. Sekiguchi, T. Akitsu, S. Shoji

CIAiS (Research and Education Consortium for Innovation of Advanced Integrated Science) International Symposium 20182018年03月

詳細

国際会議ポスター発表

超撥水性二重凹型ピラーアレイデバイスによるZn(II)錯体含有リゾチームの結晶化

田中大器、川久保渉、尹棟鉉、関口哲志、秋津貴城、庄子習一

第34回「センサ・マイクロマシンと応用システム」シンポジウム2017年10月

詳細

国内会議ポスター発表

マイクロ流体デバイスによる高効率かつ安全なブロモ基付加操作

川久保渉、田中大器、尹棟鉉、関口哲志、高橋啓太、秋津貴城、庄子習一

第34回「センサ・マイクロマシンと応用システム」シンポジウム2017年10月

詳細

国内会議ポスター発表

セルロースナノファイバーゲルを培養場とした多層ファイバー中での筋管形成誘導

今任景一、米田憲司、田中智、尹棟鉉、関口哲志、庄子習一、武田直也

第17回日本再生医療学会総会2018年03月

詳細

国内会議口頭発表(一般)

圧電薄膜フィルムを用いた安価な振動発電素子

笹川健太、佐々木敏夫、関口哲志、中島啓、庄子習一

4大学ナノ・マイクロファブリケーションコンソーシアム・シンポジウム2018年03月

詳細

国内会議ポスター発表

LCチップ集積に向けたポストカラムミキサーの開発

菅谷拓央、中西完貴、磯川宗生、尹棟鉉、関口哲志、庄子習一、船津高志、角田誠

4大学ナノ・マイクロファブリケーションコンソーシアム・シンポジウム

詳細

国内会議ポスター発表

Development of a Simple Fabrication Process for a Printable Piezoelectric Energy Harvest Device

Y. Kamata, D.H. Yoon, T. Sasaki, Y. Nozaki, S. Yamaura, T. Sekiguchi, T. Nakajima, S. Shoji

The 11th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2016)2016年04月

詳細

国際会議口頭発表(一般)

Fabrication Process of Fluidic Devices for Producing Fine Droplets

Y. Nozaki, T. Kanai, A. Matsuo, D. Tanaka, I. Yuito, T. Takeuchi, T. Sekiguchi, S. Shoji

The 16th International Conference on Nanotechnology, (IEE NANO 2016)2016年08月

詳細

国際会議口頭発表(一般)

Fifty-Second Analysis of Oxidative Stress Markers in Human Plasma Using Amide-Modified Liquid Chromatography Chip

M. Isokawa, K. Nakanishi, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda

The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2016)2016年10月

詳細

国際会議ポスター発表

In Vitro Selection of Novel Peptide Agonists for Human Somatostatin Receptor Subtype-2 Using a Water-In-Oil Microdroplet Platform

T. Sakurai, R. Iizuka, Y. Nakamura, J. Ishii, A. Kondo, A. Iguchi, D.H. Yoon, T. Sekiguchi, S. Shoji, T. Funatsu

The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2016)2016年10月

詳細

国際会議ポスター発表

Complex Check-Like Cross-Sectional Flow Formation for Fiber-Shaped Materials

K. Kobayashi, D.H. Yoon, T. Sekiguchi, S. Shoji

The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2016)2016年10月

詳細

国際会議ポスター発表

三次元マイクロ流体デバイスを用いたマルチコア螺旋構造ファイバの作製

尹棟鉉、有吉理沙、佐藤成晃、関口哲志、庄子習一

電気学会センサ・マイクロマシン部門総合研究会2016年06月

詳細

国内会議口頭発表(一般)

脳標本のリアルタイム評価のためのバイオモニタリングシステムの開発

根岸周也、鎌田将仁、梶原利一、金子祐史、尹棟鉉、関口哲志、庄子習一、工藤寛之

電気学会センサ・マイクロマシン部門総合研究会2016年06月30日

詳細

国内会議口頭発表(一般)

インクジェット工法を用いた環境発電素子の作製

鎌田裕樹、佐々木敏行、関口哲志、庄子習一

東北大学金属材料研究所・ナノ・ライフ創新研究機構 連携協定締結記念シンポジウム2016年09月01日

詳細

国内会議ポスター発表

Study for Simple Fabrication Process of Printable Piezoelectric Energy Harvest Device

鎌田裕樹、尹棟鉉、佐々木敏夫、野崎義人、山浦真一、関口哲志、中嶋宇史、庄子習一

第33回「センサ・マイクロマシンと応用システム」シンポジウム2016年10月24日

詳細

国内会議ポスター発表

二重四角構造ピラーアレイを応用した超撥水性表面の低コスト量産プロセスの開発

中西完貴、簔城森幸、尹棟鉉、関口哲志、庄子習一

第33回「センサ・マイクロマシンと応用システム」シンポジウム2016年10月25日

詳細

国内会議ポスター発表

機能性ファイバー材料合成を目的としたチェック状断面構造層流デバイスの開発

小林和博、尹棟鉉、関口哲志、庄子習一

第33回「センサ・マイクロマシンと応用システム」シンポジウム2016年10月25日

詳細

国内会議ポスター発表

FeCo系合金の逆磁歪効果を用いた小型発電素子

笹川健太、野崎義人、関口哲志、佐々木敏夫、山浦真一、中嶋宇史

4大学ナノ・マイクロファブリケーションコンソーシアム・シンポジウム2017年03月13日

詳細

国内会議ポスター発表

Fabrication of the Microfluidic Device for Chemical Reaction

S. Sawai, D. Tanaka, D.H. Yoon, T. Sekiguchi, T. Akitsu, S. Shoji

4大学ナノ・マイクロファブリケーションコンソーシアム・シンポジウム2017年03月13日

詳細

国内会議ポスター発表

早大 MEMS技術の紹介と小型発電デバイスへの応用例

庄子習一、関口哲志、水野潤

東北大学金属材料研究所・ナノ・ライフ創新研究機構 連携協定締結記念シンポジウム招待有り2016年09月01日

詳細

国内会議口頭発表(招待・特別)

In Situ Monitoring Chemical Reaction in Microdroplet

T. Fukuda, T. Kurabayashi, H. Udaka, D.H. Yoon, M. Suzuki, A. Nakahara, T. Sekiguchi, S. Shoji

EMN (Energy Materials Nanotechnology) Meeting on Droplets2015年05月08日

詳細

国際会議口頭発表(一般)

High Efficient Synthesis of Manganese(II), Cobalt(II) Complexes Containing Lysozyme Using Reaction Area Separated Micro Fluidic Device

D. Tanaka, Y. Murakoshi, E. Tsuda, Y. Mitsumoto, D.H. Yoon, T. Sekiguchi, T. Akitsu, S. Shoji

The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)2015年06月23日

詳細

国際会議口頭発表(一般)

Single Cell Encapsulated Microdroplet Formation Using TIP-Streaming and Deterministic Lateral Displacement

Y. Kamata, D.H. Yoon, T. Sekiguchi, S. Shoji

he 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2015)2015年10月26日

詳細

国際会議ポスター発表

Multi-Stage Size Dependent Passive Droplet Sorting Using Droplet Transfer on Cascade Line-Rails and Shape Recovery on Dot-Rails

Z. Xie, D.H. Yoon, T. Sekiguchi, S. Shoji

The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2015)2015年10月27日

詳細

国際会議口頭発表(一般)

Culture-Independent Method for Screening and Identifying Microbial Enzyme-Encoding Genes Using Microdroplet-Based Single Cell Genomics

K. Nakamura, R. Iizuka, T. Yoshida, Y. Hatada, Y. Takaki, S. Nishi, A. Iguchi, D.H. Yoon, T. Sekiguchi, S. Shoji, T. Funatsu

The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2015)2015年10月27日

詳細

国際会議ポスター発表

On-Chip Integration of Solid-Phase-Extraction and Silicon Pillar Arrays for High Efficient Liquid Chromatography

K Nakanishi, K. Shih, T. Kanamori, D.H. Yoon, T. Funatsu, M. Tsunoda, T. Sekiguchi, S. Shoji

The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2015)2015年10月28日

詳細

国際会議ポスター発表

All-Round Micro Sheath Flow Formation to Realize Complex Cross Sections by Simply Stacked PDMS Structures

D.H. Yoon, L. Ariyoshi, D. Tanaka, T. Sekiguchi, S. Shoji

The 29th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2016)2016年01月26日

詳細

国際会議口頭発表(一般)

外部磁場で制御可能な回転式マイクロアクチュエータの作製とマイクロ液滴搬送への応用

中西完貴、尹棟鉉、関口哲志、庄子習一

第32回「センサ・マイクロマシンと応用システム」シンポジウム2015年10月30日

詳細

国内会議ポスター発表

チップストリーミングを用いた単一細胞解析に向けた分離可能な液滴の生成

鎌田祐樹、尹棟鉉、関口哲志、庄子習一

第32回「センサ・マイクロマシンと応用システム」シンポジウム2015年10月30日

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国内会議ポスター発表

マイクロフルイディックエンジニアリングの進展と課題

庄子習一、尹棟鉉、関口哲志

新化学技術推進協会(JACI) 電子情報技術部会 マイクロナノシステムと材料・加工分科会 講演会招待有り2015年11月04日

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国内会議公開講演

Design Optimization and Fabrication of Simple Printable Piezoelectric Energy Harvest Device

K. Sasagawa, D.H. Yoon, T. Sekiguchi, T. Sasaki, T. Nakajima, S. Shoji

The 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2018)(IEEE)2018年04月

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国際会議口頭発表(一般)開催地:Singapore

High Efficiency Synthesis of Br Addition Ligand by Microfluidic Device

D. Tanaka, W. Kawakubo, K. Takahashi, D.H. Yoon,T. Sekiguchi, T. Akitsu, S. Shoji

43rd International Conference on Coordination Chemistry (ICCC 2018)2018年07月

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国際会議口頭発表(一般)開催地:Sendai

Fabrication of Long Luminant Tissues in Three-Dimensional Gel Scaffolds with Oriented Cellulose Nanofibers

T. Tanaka, K. Imato, K. Yoneda, D.H. Yoon, T. Sekiguchi, S. Shoji, N. Takeda

5th Tissue Engineering and Regenerative Medicine International Society World Congress 2018, (5th TERMIS World Congress 2018)2018年09月

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国際会議ポスター発表開催地:Kyoto

Fabrication of Long Myotube Bundles in Three-Dimensional Gel Scaffolds with Oriented Cellulose Nanofibers

K. Imato, K. Yoneda, T. Tanaka, D.H. Yoon, T. Sekiguchi, S. Shoji, N. Takeda

5th Tissue Engineering and Regenerative Medicine International Society World Congress 2018, (5th TERMIS World Congress 2018)2018年09月

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国際会議ポスター発表

Oil-In-Water Droplet Formation in Hydrophobic PDMS Device Using Three-Dimensional Protruded Taper Channel

C. Tang, D.H. Yoon, T-Sekiguchi, S, Shoj

The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2018)(IEEE)2018年11月

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国際会議ポスター発表開催地:Kaohsiung

High-Efficiency Dibromination of Organic Compound in Microfluidic Channel of Si Pillar Array Directly Coated with Iron Catalyst

T. Sugaya, D. Tanaka, D.H. Yoon, Y. Nozaki, T. Sekiguchi, T. Akitsu, S. Shoji

The 20th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2019)2019年06月

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国際会議ポスター発表

High Efficient Synthesis of Functional Compounds by Microfluidic Device

D. Tanaka, D.H. Yoon, Y. Nozaki, T. Sekiguchi, T. Akitsu, S. Shoji,

The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-4)招待有り2019年10月03日

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国際会議口頭発表(招待・特別)開催地:Sendai, Japan

Construction of a Well-Operable Crystallization Method for Chemical Analysis Applying Microdroplet Device

S. Hattori, D. Tanka, D.H. Yoon, T. Sekiguchi, Y. Nozaki, T. Akitsu, S. Shoji

The 4th Conference on Micro Fluidic Handling Systems2019年10月

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国際会議口頭発表(一般)開催地:Enschede, The Netherlands

Establishment of Labo-In-A-Microdroplet for Azo Compound Synthesis

D. Tanaka, S. Sawai, T. Sugaya, Y. Nozaki, D.H. Yoon, T. Isano, H. Yamagata, H. Fujita, T. Sekiguchi, T. Akitsu, S. Shoji

The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences2019年10月

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国際会議ポスター発表開催地:Basel, Switzerland

Simultaneous Microdroplets Generation by Tall Breakup Induced with Multi-Branch Channel

S. Kajiya, D.H. Yoon, Y. Nozaki, T. Isano, H. Yamagata, H. Fujita, T. Sekiguchi, S. Shoji

The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences2019年10月

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国際会議ポスター発表開催地:Basel, Switzerland

Sequential Formation of Daughter Droplets by Breakup of Microdroplets into Bypass Channel

S. Hattori, D.H. Yoon, Y. Nozaki, T, Isano, H. Yamagata, H. Fujita, T. Sekiguchi, S. Shoji

The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences2019年10月

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国際会議ポスター発表開催地:Basel, Switzerland

化学合成への応用に向けた有機溶媒のマイクロドロップレット形成手法の構築

湯成河、服部翔平、野崎義人、尹棟鉉、田中大器、藤田博之、秋津貴城、関口哲志、庄子習一

日本化学会 第100春季年会(日本化学会)2020年03月

圧電ポリマー材料を用いた振動発電の応用例

関口哲志、佐々木敏夫、野崎義人、庄子習一、瀧口徹、鎌田裕樹、笹川健太、小島大知、菅谷拓央

早稲田大学 エネルギーハーベスティング コンソーシアム・アンビエントロニクス研究所 合同シンポジウム(早稲田大学 エネルギーハーベスティング コンソーシアム・アンビエントロニクス研究所)招待有り2019年12月

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国内会議口頭発表(招待・特別)開催地:早稲田大学、東京

マイクロ・ナノ流体工学の光学・バイオ・化学への応用

庄子習一、桑江博之、尹棟鉉、田中大器、関口哲志、水野潤、野崎義人、笠原崇史、秋津貴城

電子情報通信学会 システムナノ技術に関する特別研究専門委員会(SNT)第3回研究会(電子情報通信学会)招待有り2020年01月

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国内会議口頭発表(招待・特別)開催地:東京

特許

整理番号:1999

圧電型発電装置及びその製造方法(日本)

関口 哲志, 佐々木 敏夫, 笹川 健太, 中嶋 宇史

特願2018- 3881、特開2019-125630

整理番号:2030

ピラーアレイミキサー及びこれを使用した試料処理装置(日本)

庄子 習一, 関口 哲志, 中西 完貴

特願2018- 18368、特開2018-122295

整理番号:200-JP

電界効果型トランジスタおよび集積回路(日本)

水野 潤, 川原田 洋, 関口 哲志, 齋藤 美紀子

特願2011-532953、特許第5648812号

外部研究資金

科学研究費採択状況

研究種別:基盤研究(S)

マイクロフルイディックエンジニアリングの深化と生体分子高感度定量計測への展開

2011年-2015年

研究分野:計測工学

配分額:¥215930000

研究種別:特別推進研究

電気化学デバイス工学の確立と深化

2008年-2012年

配分額:¥570440000

研究種別:基盤研究(A)

生体一分子リアルタイム機能解析のためのマイクロ流体システムの構築

2007年-2010年

研究分野:計測工学

配分額:¥48620000

研究種別:基盤研究(A)

マイクロナノバイオトランスポート機構を基軸にした肝臓組織再生

2013年-2016年

研究分野:熱工学

配分額:¥36010000

研究種別:

細胞内微小サンプル計測を目的としたマイクロ・ナノドロップレットハンドリング

2016年-0月-2019年-0月

配分額:¥44980000

研究種別:

マイクロナノバイオトランスポート機構を基軸にした肝臓組織再生

2013年-0月-2017年-0月

配分額:¥44720000

研究種別:基盤研究(A)

三次元マイクロ流体デバイスを応用した多重微小界面の構築と機能性化学反応への応 用

2020年-2022年

研究分野:ナノマイクロ科学およびその関連分野

配分額:¥500000

研究資金の受入れ状況

実施形態:受託教育

早稲田大学カスタムナノ造形・デバイス評価支援事業2007年-

実施形態:その他

マイクロフルイディックエンジニアリングの深化と生体分子高感度定量計測への展開2011年-

実施形態:共同研究

低炭素社会の実現に向けた人材育成ネットワークの構築と先進環境材料・デバイス創製(GRENE)2011年-

実施形態:共同研究

ダイヤモンドMOSFETを使用したグリーンインバータ開発による低炭素社会の実現2010年-

実施形態:受託教育

大口径ダイヤモンド基板によるグリーンインバータ基礎技術2010年-

実施形態:共同研究

4大学ナノ・マイクロファブリケーションコンソーシアム2010年-

実施形態:共同研究

特異構造金属・無機融合高機能材料開発共同研究プロジェクト2010年-

実施形態:その他

ナノテクノロジー要素技術養成プログラム2004年-2009年

実施形態:受託教育

地域結集型研究開発プログラム「都市の安全・安心を支える環境浄化技術開発」2006年-

現在担当している科目

科目名開講学部・研究科開講年度学期
MEMS基幹理工学部2020春学期
MEMS大学院基幹理工学研究科2020春学期
MEMS大学院先進理工学研究科2020春学期

教育内容・方法の工夫

センサ工学

2005年09月-

医用電子工学

2005年04月-