氏名

リ テンジョ

李 天舒

職名

次席研究員(研究院講師) (https://researchmap.jp/7000007373/)

所属

(理工学術院総合研究所)

連絡先

URL等

研究者番号
50740536

論文

In vitro and in vivo evaluation of maleimide-modified liposome for drug delivery

Li, Tianshu;Takeoka, Shinji

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY2452013年-2013年

WoS

詳細

ISSN:0065-7727

A novel application of maleimide for advanced drug delivery: in vitro and in vivo evaluation of maleimide-modified pH-sensitive liposomes

Li, Tianshu;Takeoka, Shinji

INTERNATIONAL JOURNAL OF NANOMEDICINE8p.3855 - 38662013年-2013年

DOIWoS

詳細

ISSN:1178-2013

Enhanced cellular uptake of maleimide-modified liposomes via thiol-mediated transport

Li, Tianshu;Takeoka, Shinji

INTERNATIONAL JOURNAL OF NANOMEDICINE9p.2849 - 28612014年-2014年

DOIWoS

詳細

ISSN:1178-2013

Construction and evaluation of pH-sensitive immunoliposomes for enhanced delivery of anticancer drug to ErbB2 over-expressing breast cancer cells

Li, Tianshu; Amari, Takuya; Semba, Kentaro; Yamamoto, Tadashi; Takeoka, Shinji; Takeoka, Shinji

Nanomedicine: Nanotechnology, Biology, and Medicine13(3)p.1219 - 12272017年04月-2017年04月 

PubMedDOIScopus

詳細

ISSN:15499634

概要:© 2016 Elsevier Inc. 1,5-Dihexadecyl N,N-diglutamyl-lysyl-L-glutamate (GGLG) liposomes were previously developed to enhance drug delivery efficiency in tumor cells owing to its pH-responsive properties. Herein, we report the modification of GGLG liposomes by conjugating a Fab′ fragment of an ErbB2 antibody to the terminus of PEG (polyethylene glycol)-lipid (Fab′-GGLG liposomes). The conjugation of Fab′ fragments did not affect the antibody activity, drug (doxorubicin, DOX) encapsulation efficiency, stability during storage or pH-sensitivity. However, the binding affinity of Fab′-GGLG liposomes was enhanced to ErbB2-overexpressing HCC1954 cells specifically, and the cell association increased 10-fold in comparison to GGLG liposomes. Consequently, intracellular DOX delivery was enhanced, with an increased cytotoxicity in HCC1954 cells (i.e., IC 50 of 1.17 and 3.08 μg/mL for Fab′-GGLG-DOX and GGLG-DOX liposomes, respectively). Further, a significantly enhanced tumor growth inhibition was obtained in an ErbB2-overexpressing breast cancer-bearing mouse model. Therefore, a potent anticancer drug delivery system was constructed by the immunological modification of pH-sensitive liposomes.

Effect of the nanoformulation of siRrNAa-lipid assemblies on their cellular uptake and immune stimulation

Kubota, Kohei; Kubota, Kohei; Onishi, Kohei; Sawaki, Kazuaki; Li, Tianshu; Mitsuoka, Kaoru; Sato, Takaaki; Takeoka, Shinji; Takeoka, Shinji; Takeoka, Shinji

International Journal of Nanomedicine12p.5121 - 51332017年07月-2017年07月 

PubMedDOIScopus

詳細

ISSN:11769114

概要:© 2017 Kubota et al. Two lipid-based nanoformulations have been used to date in clinical studies: lipoplexes and lipid nanoparticles (LNPs). In this study, we prepared small interfering RNA (siRNA)-loaded carriers using lipid components of the same composition to form molecular assemblies of differing structures, and evaluated the impact of structure on cellular uptake and immune stimulation. Lipoplexes are electrostatic complexes formed by mixing preformed cationic lipid liposomes with anionic siRNA in an aqueous environment, whereas LNPs are nanoparticles embedding siRNA prepared by mixing an alcoholic lipid solution with an aqueous siRNA solution in one step. Although the physicochemical properties of lipoplexes and LNPs were similar except for small increases in apparent size of lipoplexes and zeta potential of LNPs, siRNA uptake efficiency of LNPs was significantly higher than that of lipoplexes. Furthermore, in the case of LNPs, both siRNA and lipid were effectively incorporated into cells in a co-assembled state; however, in the case of lipoplexes, the amount of siRNA internalized into cells was small in comparison with lipid. siRNAs in lipoplexes were thought to be more likely to localize on the particle surface and thereby undergo dissociation into the medium. Inflammatory cytokine responses also appeared to differ between lipoplexes and LNPs. For tumor necrosis factor-a, release was mainly caused by siRNA. On the other hand, the release of interleukin-1β was mainly due to the cationic nature of particles. LNPs released lower amounts of tumor necrosis factor-a and interleukin-1β than lipoplexes and were thus considered to be better tolerated with respect to cytokine release. In conclusion, siRNA-loaded nanoformulations effect their cellular uptake and immune stimulation in a manner that depends on the structure of the molecular assembly; therefore, nanoformulations should be optimized before extending studies into the in vivo environment.

Preparation, characterization, and preliminary in vitro testing of nanoceria-loaded liposomes

Grillone, Agostina; Li, Tianshu; Battaglini, Matteo; Battaglini, Matteo; Scarpellini, Alice; Prato, Mirko; Takeoka, Shinji; Ciofani, Gianni; Ciofani, Gianni

Nanomaterials7(9)2017年09月-2017年09月 

DOIScopus

詳細

概要:© 2017 by the authors. Licensee MDPI, Basel, Switzerland. Cerium oxide nanoparticles (nanoceria), well known for their pro- and antioxidant features, have been recently proposed for the treatment of several pathologies, including cancer and neurodegenerative diseases. However, interaction between nanoceria and biological molecules such as proteins and lipids, short blood circulation time, and the need of a targeted delivery to desired sites are some aspects that require strong attention for further progresses in the clinical application of these nanoparticles. The aim of this work is the encapsulation of nanoceria into a liposomal formulation in order to improve their therapeutic potentialities. After the preparation through a reverse-phase evaporation method, size, Z-potential, morphology, and loading efficiency of nanoceria-loaded liposomes were investigated. Finally, preliminary in vitro studies were performed to test cell uptake efficiency and preserved antioxidant activity. Nanoceria-loaded liposomes showed a good colloidal stability, an excellent biocompatibility, and strong antioxidant properties due to the unaltered activity of the entrapped nanoceria. With these results, the possibility of exploiting liposomes as carriers for cerium oxide nanoparticles is demonstrated here for the first time, thus opening exciting new opportunities for in vivo applications.

Lysine-containing cationic liposomes activate the NLRP3 inflammasome: Effect of a spacer between the head group and the hydrophobic moieties of the lipids

Li, Tianshu; He, Jieyan; Horvath, Gabor; Próchnicki, Tomasz; Latz, Eicke; Takeoka, Shinji; Takeoka, Shinji

Nanomedicine: Nanotechnology, Biology, and Medicine14(2)p.279 - 2882018年02月-2018年02月 

DOIScopus

詳細

ISSN:15499634

概要:© 2017 Elsevier Inc. Cationic lipids containing lysine head groups and ditetradecyl, dihexadecyl or dioctadecyl glutamate hydrophobic moieties with/without propyl, pentyl or heptyl spacers were applied for the preparation of cationic liposomes using a simple bath type-sonicator. The size distribution, zeta potential, cellular internalization, and cytotoxicity of the liposomes were characterized, and the innate immune stimulation, e.g., the NLRP3 inflammasome activation of human macrophages and THP-1 cells, was evaluated by the detection of IL-1β release. Comparatively, L3C14 and L5C14 liposomes, made from the lipids bearing lysine head groups, ditetradecyl hydrophobic chains and propyl or pentyl spacers, respectively, were the most potent to activate the NLRP3 inflammasome. The possible mechanism includes endocytosis of the cationic liposomes and subsequent lysosome rupture without significant inducement of reactive oxygen species production. In summary, we first disclosed the structural effect of cationic liposomes on the NLRP3 inflammasome activation, which gives an insight into the application of nanoparticles for improved immune response.

A new strategy for the synthesis of 3-deazaneplanocin A

Li, Tianshu; Xing, Lei; Lin, Guichun; Guan, Zhu; Yang, Zhenjun

Journal of Chinese Pharmaceutical Sciences査読有り19(6)p.436 - 4422010年11月-2010年11月 

DOIlink

詳細

掲載種別:研究論文(学術雑誌)ISSN:1003-1057

書籍等出版物

Smart Nanoparticles for Biomedicine

Gianna Ciofani(分担執筆)

Elsevier2018年 05月-2018年 05月

詳細

単行本(学術書)総ページ数:268担当ページ数:31-47ISBN:9780128141564

外部研究資金

科学研究費採択状況

研究種別:

サイトゾルデリバリーに特化したゲノム編集用ナノ粒子の構築

2019年-0月-2022年-0月

配分額:¥4160000

現在担当している科目

科目名開講学部・研究科開講年度学期
Science and Engineering Laboratory基幹理工学部2020秋学期
Science and Engineering Laboratory 1B基幹理工学部2020秋学期
理工学基礎実験1B Vブロック基幹理工学部2020秋学期
Science and Engineering Laboratory創造理工学部2020秋学期
Science and Engineering Laboratory 1B創造理工学部2020秋学期
理工学基礎実験1B Vブロック創造理工学部2020秋学期
Science and Engineering Laboratory先進理工学部2020秋学期
Science and Engineering Laboratory 1B先進理工学部2020秋学期
理工学基礎実験1B Vブロック先進理工学部2020秋学期
Chemistry and Bioscience Laboratory先進理工学部2020秋クォーター
Chemistry and Bioscience Laboratory先進理工学部2020秋クォーター
Chemistry and Bioscience Laboratory [S Grade]先進理工学部2020秋クォーター
Chemistry and Bioscience Laboratory [S Grade]先進理工学部2020秋クォーター
Current Topics in Biosciences先進理工学部2020秋学期
Current Topics in Biosciences [S Grade]先進理工学部2020秋学期
Fundamental Bioscience Laboratory先進理工学部2020春学期
Life Science and Medical Bioscience Laboratory先進理工学部2020春学期
Molecular Cell Biology B先進理工学部2020春学期
Molecular Cell Biology B先進理工学部2020春学期