氏名

ニュウノヤ ヒロシ

丹生谷 博

職名

講師(任期付)

所属

(創造理工学部)

プロフィール

実験教育センター講師

連絡先

メールアドレス

メールアドレス
nyunoya@aoni.waseda.jp

学歴・学位

学位

理学博士 課程 東京大学 植物分子・生理科学

論文

Altered Subcellular Localization of a Tobacco Membrane Raft-Associated Remorin Protein by Tobamovirus Infection and Transient Expression of Viral Replication and Movement Proteins

Sasaki N, Takashima E, Nyunoya H

Frontiers in plant science査読有り9(619)2018年05月-

link

詳細

概要:Remorins are plant specific proteins found in plasma membrane microdomains (termed lipid or membrane rafts) and plasmodesmata. A potato remorin is reported to be involved in negatively regulating potexvirus movement and plasmodesmal permeability. In this study, we isolated cDNAs of tobacco remorins (NtREMs) and examined roles of an NtREM in infection by tomato mosaic virus (ToMV). Subcellular localization analysis using fluorescently tagged NtREM, ToMV, and viral replication and movement proteins (MPs) indicated that virus infection and transient expression of the viral proteins promoted the formation of NtREM aggregates by altering the subcellular distribution of NtREM, which was localized uniformly on the plasma membrane under normal conditions. NtREM aggregates were often observed associated closely with endoplasmic reticulum networks and bodies of the 126K replication and MPs. The bimolecular fluorescence complementation assay indicated that NtREM might interact directly with the MP on the plasma membrane and around plasmodesmata. In addition, transient overexpression of NtREM facilitated ToMV cell-to-cell movement. Based on these results, we discuss possible roles of the tobacco remorin in tobamovirus movement.

Introns of the tobacco resistance gene N play important roles in elicitor-responsive upregulation and efficient induction of defense responses

Taku, Kazuo; Sasaki, Nobumitsu; Matsuzawa, Kenta; Okamura-Mukai, Atsushi; Nyunoya, Hiroshi

Journal of General Plant Pathology査読有り84(2)p.73 - 842018年03月-2018年03月 

DOIScopus

詳細

ISSN:13452630

概要:© 2018, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature. The tobacco resistance gene N is known to be upregulated transcriptionally upon infection with Tobacco mosaic virus or the transient expression of the virus elicitor (termed p50), which is the helicase domain of the virus replicase, leading to cell death-associated defense responses. To investigate the roles of the encoded protein and introns of the N gene in its elicitor-triggered upregulation, we developed an Agrobacterium-mediated transient gene transfer system to express the N transgene controlled by the upstream region of the N gene itself in N-lacking tobacco. The transcript level of the N transgene with the introns was enhanced sharply when the p50 transgene was coexpressed. Introduction of a frameshift mutation in the intron-containing N transgene abolished its response to the elicitor, but an additional expression of the functional N protein supplied in trans complemented the upregulation of the mutant transgene. In contrast, an intron-less N transgene failed to be upregulated by the p50 coexpression, resulting in delayed and attenuated cell death and slow induction of defense-related gene expression. In addition, introduction of a frameshift mutation in the intron-less N transgene resulted in no response to the elicitor even with coexpression of the functional N protein. Our data provide the first evidence for important roles of introns of a resistance gene in elicitor-responsive gene regulation and efficient defense induction.

Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement: Far-Western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein.

Sasaki N, Matsushita Y, Nyunoya H

In "(book) Antiviral Resistance in Plants : Methods and Protocols", Methods in Molecular Biology2028p.123 - 1442019年06月-

DOIlink

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社会貢献活動

農水省農業資材審議会臨時委員
詳細

概要:遺伝子組換え飼料の安全性評価

多能工型研究支援人材育成コンソーシアム教育プログラム講師

主催者・媒体名:群馬大学・茨城大学・宇都宮大学

詳細

概要:講座「カルタヘナ法について」の講師