氏名

アサヒ　トオル

朝日　透

職名

教授 (https://researchmap.jp/read0206470/)

所属

（先進理工学部）

連絡先

メールアドレス

メールアドレス: tasahi@waseda.jp

住所・電話番号・fax番号

住所: 〒162-8480新宿区若松町2-2 早稲田大学先端生命医科学センター
電話番号: 03-5369-7327
fax番号: 03-5369-7327

URL等

WebページURL

http://asahi-lab.jp/index.html（早稲田大学生物物性科学研究室（朝日研究室））

研究者番号

80222595

本属以外の学内所属

兼担

理工学術院（大学院先進理工学研究科）

研究院（研究機関）/附属機関・学校（グローバルエデュケーションセンター）

国際学術院（国際教養学部）

学内研究所等

欧州バイオメディカルサイエンス研究所

プロジェクト研究所所長 2009年-2011年

理工学総合研究センター

兼任研究員 2003年-2006年

ビジネス・テクニカル・コミュニケーション研究所

研究所員 2013年-2016年

欧州バイオメディカルサイエンス研究所

研究所員 2009年-2011年

ライフサポートイノベーション研究所

研究所員 2014年-2014年

知的財産拠点形成研究所

研究所員 2009年-2013年

欧州バイオメディカルサイエンス研究所

プロジェクト研究所所長 2011年-2012年

欧州バイオメディカルサイエンス研究所

研究所員 2011年-2012年

東日本大震災復興研究拠点・先端環境医工科学研究所

研究所員 2011年-2013年

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2015年-2016年

欧州バイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2012年-2014年

規範科学総合研究所

研究所員 2014年-2014年

地域間研究所

研究所員 2015年-2015年

先端ベンチャー・起業家研究所

研究所員 2015年-2018年

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2016年-2017年

欧州バイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2015年-2016年

グローバルバイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2017年-2018年

グローバルバイオメディカルグリーンサイエンス研究所

研究所員 2017年-2018年

グローバルバイオメディカルグリーンサイエンス研究所

プロジェクト研究所所長 2016年-2017年

各務記念材料技術研究所

兼任研究員 2017年-2018年

理工学術院総合研究所（理工学研究所）

兼任研究員 2006年-2018年

知的財産法制研究所

研究所員 2014年-2019年

ライフサポートイノベーション研究所

研究所員 2015年-2019年

規範科学総合研究所

研究所員 2015年-2019年

理工学術院総合研究所（理工学研究所）

兼任研究員 2018年-

実践的ナノ化学G

研究所員 2007年-

欧州バイオメディカルグリーンサイエンス研究所

研究所員 2012年-

ビジネス・テクニカル・コミュニケーション研究所

研究所員 2016年-

グローバル科学知融合研究所

プロジェクト研究所所長 2019年-2023年

知的財産法制研究所

研究所員 2019年-

ライフサポートイノベーション研究所

研究所員 2019年-

規範科学総合研究所

研究所員 2019年-

学歴・学位

学歴

-1986年	早稲田大学理工学部応用物理学科
-1991年	早稲田大学理工学研究科

学位

博士(理学) 早稲田大学

経営学修士（専門職）早稲田大学

経歴

1986年-	早稲田大学理工学部応用物理学科卒業
1988年-	同理工学研究科物理学及び応用物理学専攻修士課程修了
1990年-	早稲田大学理工学部助手
1992年-	同大学博士（理学）
1993年-	日本学術振興会博士特別研究員
1995年-	早稲田大学各務記念材料技術研究所助手
1997年-	同研究所客員助教授
2002年-	同大学理工学総合研究センター客員助教授
2003年-	同大学大学院理工学研究科ナノ理工学専攻客員助教授
2004年-	同大学先端科学・健康医療融合研究機構生命医療工学インスティテュート教授
2007年-	同大学理工学術院先進理工学部生命医科学科教授
	経営学修士（専門職）

所属学協会

高分子学会

応用物理学会

日本物理学会

日本化学会

日本結晶学会

日本磁気学会

日本セラミックス協会

電気化学会

日本薬学会

研究分野

キーワード

生物物性科学／キラルサイエンス／磁性薄膜

研究シーズ

光で曲がる結晶材料

シーズ分野：ナノ・材料

皮膚に優しいケミカル健康モニタ

シーズ分野：ライフサイエンス

歩いて走るロボット結晶

シーズ分野：ナノ・材料

研究テーマ履歴

サリドマイドの単結晶育成とその光学的研究

国内共同研究

溶液中におけるサリドマイド分子挙動の物理化学的解析

国内共同研究

グリシンの単結晶育成とその絶対構造の決定

国際共同研究

G-HAUP(Generalized High-Accuracy Universal Polarimeter)法による新規光学測定法の開発

個人研究

ポリ乳酸ナノシートの微細構造解析

国内共同研究

垂直磁気記録媒体の研究

国内共同研究

強誘電体及び高温超伝導体の相転移の研究

個人研究

高異方性コラーゲン薄膜の光学的キラリティの研究

国内共同研究

シンクロトロン放射光を用いた凝集物質の分光学的研究

国内共同研究

磁性薄膜の磁気円２色性の研究

国内共同研究

論文

Walking and rolling of crystals induced thermally by phase transition

Taniguchi, Takuya; Sugiyama, Haruki; Uekusa, Hidehiro; Shiro, Motoo; Asahi, Toru; Asahi, Toru; Koshima, Hideko

Nature Communications9(1)2018年12月-2018年12月　

概要：© 2018 The Author(s). The mechanical motion of materials has been increasingly explored in terms of bending and expansion/contraction. However, the locomotion of materials has been limited. Here, we report walking and rolling locomotion of chiral azobenzene crystals, induced thermally by a reversible single-crystal-to-single-crystal phase transition. Long plate-like crystals with thickness gradient in the longitudinal direction walk slowly, like an inchworm, by repeated bending and straightening under heating and cooling cycles near the transition temperature. Furthermore, thinner, longer plate-like crystals with width gradient roll much faster by tilted bending and then flipping under only one process of heating or cooling. The length of the crystal is shortened above the transition temperature, which induces bending due to the temperature gradient to the thickness direction. The bending motion is necessarily converted to the walking and rolling locomotion due to the unsymmetrical shape of the crystal. This finding of the crystal locomotion can lead to a field of crystal robotics.

The Neuroprotective Effect of Thalidomide against Ischemia through the Cereblon-mediated Repression of AMPK Activity

Sawamura, Naoya; Sawamura, Naoya; Yamada, Mariko; Fujiwara, Miku; Yamada, Haruka; Hayashi, Hideki; Takagi, Norio; Asahi, Toru; Asahi, Toru

Scientific Reports8(1)2018年12月-2018年12月　

概要：© 2018 The Author(s). Thalidomide was originally used as a sedative and found to be a teratogen, but now thalidomide and its derivatives are widely used to treat haematologic malignancies. Accumulated evidence suggests that thalidomide suppresses nerve cell death in neurologic model mice. However, detailed molecular mechanisms are unknown. Here we examined the molecular mechanism of thalidomide's neuroprotective effects, focusing on its target protein, cereblon (CRBN), and its binding protein, AMP-activated protein kinase (AMPK), which plays an important role in maintaining intracellular energy homeostasis in the brain. We used a cerebral ischemia rat model of middle cerebral artery occlusion/reperfusion (MCAO/R). Thalidomide treatment significantly decreased the infarct volume and neurological deficits of MCAO/R rats. AMPK was the key signalling protein in this mechanism. Furthermore, we considered that the AMPK-CRBN interaction was altered when neuroprotective action by thalidomide occurred in cells under ischemic conditions. Binding was strong between AMPK and CRBN in normal SH-SY5Y cells, but was weakened by the addition of H 2 O 2 . However, when thalidomide was administered at the same time as H 2 O 2 , the binding of AMPK and CRBN was partly restored. These results suggest that thalidomide inhibits the activity of AMPK via CRBN under oxidative stress and suppresses nerve cell death.

Consolidation of Sm2Fe17N3magnets with Sm-based eutectic alloy binder

Otogawa, Kohei; Takagi, Kenta; Asahi, Toru

Journal of Alloys and Compounds746p.19 - 262018年05月-2018年05月　

ISSN：09258388

概要：© 2018 Elsevier B.V. Consolidation of Sm 2 Fe 17 N 3 powder with a new Sm-based alloy as a metal binder was examined. Investigation of various Sm-based alloys led to the discovery of a Sm-Fe-Cu-Al alloy having a low melting point of 495 °C, which is more than 100 °C lower than the thermal decomposition temperature of Sm 2 Fe 17 N 3 . The metallographic structure of the Sm-Fe-Cu-Al alloy ingot was a SmCu/Sm eutectic structure containing solid-solution Fe, Cu and Al. Pulverized Sm-Fe-Cu-Al powder as a binder was mixed with Sm 2 Fe 17 N 3 powder, and the mixture was hot-pressed by a current sintering apparatus. The Sm-Fe-Cu-Al alloy binder did not affect the magnetization of Sm 2 Fe 17 N 3 adversely after consolidation. The binder-added Sm 2 Fe 17 N 3 hot-pressed compacts successfully maintained the coercivity of the raw powder over a wide range of hot-pressing temperatures, whereas additive-free Sm 2 Fe 17 N 3 hot-pressed compacts showed a significant decrease in coercivity. Therefore, the Sm-Fe-Cu-Al alloy binder overcame the weakest point of Sm 2 Fe 17 N 3 magnets. XRD analysis suggested that the Sm-Fe-Cu-Al binder restrained precipitation of the α-Fe phase, which is thought to be the probable cause the decrease of coercivity.

A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin

Shitashima, Yoh; Shitashima, Yoh; Shimozawa, Togo; Asahi, Toru; Asahi, Toru; Miyawaki, Atsushi; Miyawaki, Atsushi

Biochemical and Biophysical Research Communications496(3)p.872 - 8792018年02月-2018年02月　

ISSN：0006291X

概要：© 2018 Elsevier Inc. UnaG is a green-emitting fluorescent protein that utilizes unconjugated bilirubin (BR) as its fluorophore. While BR has captured the attention of physiologists as an important antioxidant that scavenges reactive oxygen species in biological membranes, its excessive accumulation causes several clinical symptoms. Although the optimal regulation of BR concentration would result in clinical therapies for aging as well as reduce risks of clinical symptoms, UnaG hardly releases BR owing to its extremely high affinity for BR (K d = 98 pM). Herein, we engineered the BR binding and fluorescence of UnaG to be Ca 2+ -sensitive via a genetic insertion of calmodulin (CaM). The resultant UnaG/CaM hybrid protein is a dual-ligand modulable fluorescent protein; binding of the fluorogenic ligand BR is negatively regulated by the other ligand, Ca 2+ ion. The affinity for BR differed by three orders of magnitude between the Ca 2+ -free state (K d = 9.70 nM) and Ca 2+ -saturated state (K d = 9.65 μM). The chimeric protein can release nano- to micro-molar levels of BR with Ca 2+ control, and is thus named BReleaCa (BR + releaser + Ca 2+ ). Such a protein hybridization technique will be generally applicable to change the ligand binding properties of a variety of ligand-inducible functional proteins.

Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG

Shitashima, Yoh; Shitashima, Yoh; Shimozawa, Togo; Kumagai, Akiko; Miyawaki, Atsushi; Asahi, Toru; Asahi, Toru

Biophysical Journal113(12)p.2805 - 28142017年12月-2017年12月　

ISSN：00063495

概要：© 2017 Biophysical Society UnaG is a recently discovered ligand-induced fluorescent protein that utilizes bound bilirubin (BR) as its fluorophore. The fluorescence of the UnaG-BR complex (holoUnaG) compares in quantum efficiency to that of enhanced green fluorescent protein, but it is superior in that the fluorophore formation is instantaneous and not dependent on oxygen; hence, much attention has been paid to UnaG as a new fluorescent probe. However, many important molecular properties of fluorescent probes remain unknown, such as the association/dissociation rates of BR, which determine the stability thereof, and the dispersibility of UnaG in aqueous solutions, which influence the functions of labeled proteins. In this study, we found, in the process of investigating the association rate, that the holoUnaG takes two distinct fluorescence states, which we named holoUnaG 1 and holoUnaG 2 . The holoUnaG 1 initially forms after binding BR and then changes to the brighter holoUnaG 2 by a reversible intra-molecular reaction, thereby finally reaching an equilibrium between the two states. Spectroscopic analysis indicated that the intra-molecular reaction was as sociated not with a chemical change of BR but with a change in the environmental conditions surrounding BR. We also revealed that the molecular brightness ratio and equilibrium population ratio of the two states (holoUnaG 1 /holoUnaG 2 ) were 1:3.9 and 6:4, respectively, using photon number counting analysis. From these results, we have suggested a novel schema, to our knowledge, for the formation of the UnaG and BR complex system and have determined the various rate constants associated therein. Additionally, using analytical ultracentrifugation, we established that UnaG in the apo-state (apoUnaG) and the holoUnaG are monomeric in aqueous solution. These findings provide not only key information for the practical use of UnaG as a fluorescent probe, but also the possibility for development of a brighter UnaG mutant by genetic engineering to constitutive holoUnaG 2 .

Optical Activity Anisotropy of Benzil

Nakagawa, Kenta; Martin, Alexander T.; Nichols, Shane M.; Murphy, Veronica L.; Kahr, Bart; Kahr, Bart; Asahi, Toru; Asahi, Toru

Journal of Physical Chemistry C121(45)p.25494 - 255022017年11月-2017年11月　

ISSN：19327447

概要：© 2017 American Chemical Society. Optical activity (OA) along the optic axis of crystalline benzil has been measured by many over the past 150 years. However, the OA anisotropy remains uncharacterized due to difficulties in sample preparation as well as competition with linear birefringence (LB). The challenges associated with measuring OA along low-symmetry directions in crystals have too often left scientists with only average values of nonresonant OA in solution, i.e., specific rotations, which continue to resist interpretation in terms of structure. Measuring OA anisotropy has been facilitated by recent advances in polarimetry and optical modeling and here we compare results from two distinct division-of-time polarimeters. The absolute structure of crystalline benzil was established for the first time. The optical rotation (OR) of (+)-crystalline benzil (space group P3 1 21) perpendicular to the optic axis at the sodium D-line is -24.6 ± 1.1°/mm. A spectroscopic optical model in the transparent region of the crystal is provided. Electronic structure calculations of OR inform the polarimetric measur ements and point to the necessity of developing linear response theory with periodic boundary conditions in order to interpret the results of chiroptical measurements in crystals.

Block-Copolymer-Assisted Electrochemical Synthesis of Mesoporous Gold Electrodes: Towards a Non-Enzymatic Glucose Sensor

Nugraha, Asep Sugih; Nugraha, Asep Sugih; Li, Cuiling; Bo, Jiang; Bo, Jiang; Iqbal, Muhammad; Iqbal, Muhammad; Alshehri, Saad M.; Ahamad, Tansir; Malgras, Victor; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Asahi, Toru

ChemElectroChem4(10)p.2571 - 25762017年10月-2017年10月　

概要：© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Sensing technology for biomolecules has played a key role for maintaining a high quality of life. Various kinds of sensing techniques have been developed for detecting biomolecules. Among the detection techniques which have been reported so far, non-enzymatic glucose detection, based on electrochemical mechanisms, has attracted great interest as a facile route for monitoring glucose levels in the blood. In this method, designing nanostructured electrodes is a promising strategy to improve detection performance. Owing to their fascinating physicochemical properties, including superior electrical and electrocatalytic properties, mesoporous metal films offer a wide range of useful applications which cannot be achieved by other mesoporous materials. In this work, mesoporous Au films (MpGFs) are synthesized by an effective and simple electrochemical process in the presence of a surfactant, where the mesoporous structure can be controlled by the size of the polymeric micelles. The micelles are removed using a tetrahydrofuran-based solvent-extraction method. The activity of MpGFs towards glucose oxidation is evaluated under a fixed potential in alkaline solution. Under optimal potentials, the current response for other interfering molecules is minimal, while the current response for glucose is maximal. A linear relationship between the current response and the glucose content is observed over a wide range of concentrations. Thus, our MpGF shows an excellent performance for electrochemical glucose sensing applications.

Fabrication of Mesoporous Cu Films on Cu Foils and Their Applications to Dopamine Sensing

Baba, Daisuke; Baba, Daisuke; Li, Cuiling; Malgras, Victor; Jiang, Bo; Alamri, Hatem R.; Alothman, Zeid A.; Hossain, Md Shahriar A.; Hossain, Md Shahriar A.; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Asahi, Toru

Chemistry - An Asian Journal12(18)p.2467 - 24702017年09月-2017年09月　

ISSN：18614728

概要：© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We previously succeeded to prepare stable mesoporous Cu films on Au-coated conductive working electrodes by using polystyrene-b-poly(oxyethylene) (PS 63 000 -b-PEO 26 000 ) micelles as template and sulfuric acid to increase ionic conductivity. In the present study, we report the preparation of mesoporous Cu films on Cu foil. By changing the Cu salts and electrodeposition potentials, we discuss how these parameters influence the final product. Without having to filtrate interefering species, such as uric acid, ascorbic acid and glucose, the dopamine concentration can be precisely determined by applying a suitable potential. Therefore, non-invasive electrochemical sensing based on mesoporous films will be useful for daily diagnosis of mental disorder.

Chiral Sensing with Mesoporous Pd@Pt Nanoparticles

Fang, Yuxi; Fang, Yuxi; Li, Cuiling; Bo, Jiang; Bo, Jiang; Henzie, Joel; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke; Asahi, Toru

ChemElectroChem4(8)p.1832 - 18352017年08月-2017年08月　

概要：© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Mesoporous Pd@Pt nanoparticles possess high surface areas while still maintaining electroconductivity and low potential for agglomeration. By introducing L- or D-DOPA (3,4-dihydroxyphenylalanine) enantiomers during the synthesis of the mesoporous Pd@Pt nanoparticles, we can create a Pt shell that is sensitive to the chirality of the DOPA molecule. We demonstrate sensitive electrochemical detection of DOPA enantiomers.

Continuous Mesoporous Pd Films by Electrochemical Deposition in Nonionic Micellar Solution

Iqbal, Muhammad; Iqbal, Muhammad; Li, Cuiling; Wood, Kathleen; Jiang, Bo; Takei, Toshiaki; Dag, Ömer; Dag, Ömer; Baba, Daisuke; Baba, Daisuke; Nugraha, Asep Sugih; Nugraha, Asep Sugih; Asahi, Toru; Whitten, Andrew E.; Hossain, Md Shahriar A.; Malgras, Victor; Yamauchi, Yusuke; Yamauchi, Yusuke; Yamauchi, Yusuke

Chemistry of Materials29(15)p.6405 - 64132017年08月-2017年08月　

ISSN：08974756

概要：© 2017 American Chemical Society. Mesoporous metals that combine catalytic activity and high surface area can provide more opportunities for electrochemical applications. Various synthetic methods, including hard and soft templating, have been developed to prepare mesoporous/nanoporous metals. Micelle assembly, typically involved in soft-templates, is flexible and convenient for such purposes. It is, however, difficult to control, and the ordering is significantly destroyed during the metal deposition process, which is detrimental when it comes to designing precisely mesostructured materials. In the present work, mesoporous Pd films were uniformly electrodeposited using a non ionic surfactant, triblock copolymer poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), as a pore-directing agent. The interaction between micelles and metal precursors greatly influences the metal growth and determines the final structure. The water-coordinated species interact with the ethylene oxide moiety of the micelles to effectively drive the Pd(II) species toward the working electrode surface. From small-angle neutron scattering data, it is found that spherical P123 micelles, with an average diameter of ∼14 nm, are formed in the electrolyte, and the addition of Pd ions does not significantly modify their structure, which is the essence of the micelle assembly approach. The uniformly sized mesopores are formed over the entire mesoporous Pd film and have an average pore diameter of 10.9 nm. Cross-sectional observation of the film also shows mesopores spanning continuously from the bottom to the top of the film. The crystallinity, crystal phase, and electronic coordination state of the Pd film are also confirmed. Through this study, it is found that the optimized surfactant concentration and applied deposition potential are the key factors to govern the formation of homogeneous and well-distributed pores over the entire film. Interestingly, the as-prepared mesoporous Pd films exhibit superior electrocatalytic activity toward the ethanol oxidation reaction by fully utilizing the accessible active surface area. Our approach combines electrochemistry with colloidal and coordination chemistry and is widely applicable to other promising metals and alloy electrocatalysts.

LC–MS/MS and chiroptical spectroscopic analyses of multidimensional metabolic systems of chiral thalidomide and its derivatives

Ogino, Yoshiyuki; Tanaka, Masahito; Shimozawa, Togo; Asahi, Toru

Chirality29(6)p.282 - 2932017年06月-2017年06月　

ISSN：08990042

概要：© 2017 Wiley Periodicals, Inc. Enantiomeric thalidomide undergoes various kinds of biotransformations including chiral inversion, hydrolysis, and enzymatic oxidation, which results in several metabolites, thereby adding to the complexity in the understanding of the nature of thalidomide. To decipher this complexity, we analyzed the multidimensional metabolic reaction networks of thalidomide and related molecules in vitro. Characteristic patterns in the amount of various metabolites of thalidomide and related molecules generated during a combination of chiral inversion, hydrolysis, and hydroxylation were observed using liquid chromatography–tandem mass spectrometry and chiroptical spectroscopy. We found that monosubstituted thalidomide derivatives exhibited different time-dependent metabolic patterns compared with thalidomide. We also revealed that monohydrolyzed and monohydroxylated metabolites of thalidomide were likely to generate mainly by a C-5 oxidation of thalidomide and subsequent ring opening of the hydroxylated metabolite. Since chirality was conserved in most of these metabolites during metabolism, they had the same chirality as that of nonmetabolized thalidomide. Our findings will contribute toward understanding the significant pharmacological effects of the multiple metabolites of thalidomide and its derivatives.

Accurate measurement of the optical activity of alanine crystals and the determination of their absolute chirality

Ishikawa, Kazuhiko; Terasawa, Yukana; Tanaka, Masahito; Asahi, Toru; Asahi, Toru

Journal of Physics and Chemistry of Solids104p.257 - 2662017年05月-2017年05月　

ISSN：00223697

概要：© 2017 Elsevier Ltd Wavelength dependence measurements of the chiroptical properties in alanine crystals have so far been unsuccessful using conventional spectroscopic techniques. We describe our attempts to measure the wavelength dependence of the optical activity in L- and D-alanine crystals along each crystallographic axis, and to determine the absolute chirality of alanine crystals by correlating the absolute structure to the optical activity using an x-ray diffractometer and a generalized high accuracy universal polarimeter. We have succeeded in accurately measuring the optical rotatory dispersion in the < 010 > direction, which shows that the optical rotation of the D-alanine crystal is dextrorotatory and that of the L-alanine crystal is laevorotatory, thereby determining the absolute chirality. Furthermore, comparison with the optical activity in solution shows that the optical activity in alanine crystals is different not only in value, but also in the sign. These results have led us to conclude that the optical rotatory power in the crystalline state should not be simply the summation of molecular optical rotatory power values. We propose the necessity of a theory, which contains the contribution of molecular interactions within the crystal, in order to calculate the optical rotatory power of the crystalline state.

Fast-type high-accuracy universal polarimeter using charge-coupled device spectrometer

Takanabe, Akifumi; Koshima, Hideko; Asahi, Toru; Asahi, Toru

AIP Advances7(2)2017年02月-2017年02月　

概要：© 2017 Author(s). A fast, high-accuracy universal polarimeter was developed using a charge-coupled device (CCD) spectrometer (CCD-HAUP), to carry out simultaneous optical anisotropic (linear birefringence, LB; linear dichroism, LD) and chiroptical (circular birefringence, CB; circular dichroism, CD) measurements on single crystals without any pretreatment, in the visible region between 400-680 nm. The principle of the HAUP method is to measure the intensities of emergent light passing through a polarizer, a crystal sample, and then an analyzer, as the azimuth angles of the polarizer and analyzer are independently altered. The CCD-HAUP has the unique feature that white transmitted light intensity can be measured using a CCD spectrometer, compared with the generalized HAUP (G-HAUP) system in which monochromatic transmitted light is measured using a photomultiplier. The CCD-HAUP measurements across the entire wavelength region are completed within the G-HAUP measurement time for a single wavelength. The CCD-HAUP drastically reduces the measurement time for a dataset to only 1.5 h, from the 24 h required for the G-HAUP system. LB, LD, CB, and CD measurements of single crystals of α-quartz and enantiomeric photomechanical salicylidenephenylethylamines before, during, and after ultraviolet light irradiation show results comparable to those obtained using the G-HAUP system. The newly developed system is very effective for samples susceptible to degradation induced by external stimuli, such as light and heat.

Reversible single-crystal-to-single-crystal phase transition of chiral salicylidenephenylethylamine

Takanabe, Akifumi; Katsufuji, Takuro; Johmoto, Kohei; Uekusa, Hidehiro; Shiro, Motoo; Koshima, Hideko; Asahi, Toru; Asahi, Toru

Crystals7(1)2017年01月-2017年01月　

概要：© 2016 by the author; licensee MDPI, Basel, Switzerland.The chiral crystal of enantiomeric (S)-N-3,5-di-tert-butylsalicylidene-1-phenylethylamine in the enol form [enol-(S)-1] undergoes a reversible single-crystal-to-single-crystal (SCSC) phase transition at Tc ≈ 3 °C from the room temperature α-form in orthorhombic space group P212121 (Zʹ = 1) to the low temperature β-form in the monoclinic space group P21 (Zʹ = 2) with a thermal hysteresis of approximately 1.7 °C. A detailed comparison of the crystal structures of the α-and β-forms revealed that the 5-tert-butyl group of one molecule in the asymmetric unit of the β-form rotated by ca. 60°, and the dihedral angle between the phenyl and salicyl planes increased slightly in the β-form crystal. However, the changes in the molecular conformation and packing arrangement are small, which leads to the reversible SCSC phase transition with no destruction of the crystal lattice. The dielectric constant along the b-axis was small, probably due to the weak intermolecular interactions in the crystals.

Solid-state photochemical reaction of multisubstituted thymine derivatives

Udagawa, Akihiro; Johnston, Priscilla; Uekusa, Hidehiro; Koshima, Hideko; Saito, Kei; Asahi, Toru; Asahi, Toru

ACS Sustainable Chemistry and Engineering4(11)p.6107 - 61142016年11月-2016年11月　

概要：© 2016 American Chemical Society.Solid-state photochemical reactions in crystals, known as topochemical reactions, are solvent-free green chemical reactions that produce stereospecific molecules. The photoreaction of thymine is interesting because the dimeric photoproduct can form four types of stereoisomers and when the dimer is formed in DNA helices it can cause skin cancers. We investigated the photoreaction of five multisubstituted thymine derivatives in the solid-state, which were designed with crystal engineering concepts to promote π-π stacking of benzene rings in the crystal. Powder X-ray diffraction analysis revealed that a para-substituted bis-thymine derivative was aligned along the c axis in the crystal and was susceptible to topochemical reaction to form a polymer, as previously reported. Ortho- and meta-substituted bis-thymine derivatives and a tetrakis-substituted derivative were found to be topochemically unreactive using both gel permeation chromatography (GPC) and X-ray crystal structural analysis. The tris-substituted thymine derivative was found to be topochemically reactive due to favorable crystal packing, which included ethanol molecules to form hydrogen bonding with one of the thymine moieties and stabilize the crystal packing. GPC and crystal structural analysis revealed that it could form tetramer at most via topochemical [2+2]-cycloaddition upon UV irradiation. Based on the crystal structure of the tris-substituted thymine derivative, the structure of the tetrameric photoproduct is expected to link via cis-syn, trans-anti and cis-syn cyclobutane isomers.

Optical Activity and Optical Anisotropy in Photomechanical Crystals of Chiral Salicylidenephenylethylamines

Takanabe, Akifumi; Tanaka, Masahito; Johmoto, Kohei; Uekusa, Hidehiro; Mori, Tadashi; Koshima, Hideko; Asahi, Toru; Asahi, Toru

Journal of the American Chemical Society138(45)p.15066 - 150772016年11月-2016年11月　

ISSN：00027863

概要：© 2016 American Chemical Society.Introducing chirality into photomechanical crystals is beneficial for the diversification of mechanical motion. Measurement of the chiroptical and optical anisotropic properties of chiral crystals is indispensable for evaluating photomechanical crystals. The platelike crystals of S- and R-enantiomers of photochromic N-3,5-di-tert-butylsalicylidene-1-phenylethylamine in enol form (enol-(S)-1 and enol-(R)-1) caused bending motion with twisting upon ultraviolet (UV) light irradiation, due to shrinkage along the length and width directions of the irradiated surface, based on the optimized crystal structure of the photoisomerized trans-keto-(S)-1. By employing the generalized high-accuracy universal polarimeter (G-HAUP), optical anisotropic (linear birefringence, LB; linear dichroism, LD) as well as chiroptical (circular birefringence, CB; circular dichroism, CD) spectra of both the enantiomeric crystals on the (001) face were simultaneously measured before and under continuous UV irradiation. The LD peak was observed at 330 nm in the negative sign, derived from the π-π∗ transition of the intramolecularly hydrogen-bonded salicylidenimino moiety. The CD spectra of the S and R crystals revealed the negative and positive Cotton effect at 330 nm, respectively, and new peaks appeared at 460 nm under UV light irradiation due to photoisomerization to the S and R trans-keto isomers at around 10% conversion. The CB and CD spectra evaluated by the HAUP measurement were opposite to those measured in the hexane solution, as well as those simulated by quantum chemical calculation. The dissymmetry parameter, g, of the enol-(S)-1 crystal along the c axis (0.013) was approximately 10 times larger than the g values in the solution (0.0010) and by calculation (0.0016).

Ohgata, the single Drosophila ortholog of human cereblon, regulates insulin signaling-dependent organismic growth

Wakabayashi, Satoru; Sawamura, Naoya; Sawamura, Naoya; Voelzmann, André; Broemer, Meike; Asahi, Toru; Asahi, Toru; Hoch, Michael

Journal of Biological Chemistry291(48)p.25120 - 251322016年11月-2016年11月　

ISSN：00219258

概要：© 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Cereblon (CRBN) is a substrate receptor of the E3 ubiquitin ligase complex that is highly conserved in animals and plants. CRBN proteins have been implicated in various biological processes such as development, metabolism, learning, and memory formation, and their impairment has been linked to autosomal recessive non-syndromic intellectual disability and cancer. Furthermore, human CRBN was identified as the primary target of thalidomide teratogenicity. Data on functional analysis of CRBN family members in vivo, however, are still scarce. Here we identify Ohgata (OHGT), the Drosophila ortholog of CRBN, as a regulator of insulin signaling-mediated growth. Using ohgt mutants that we generated by targeted mutagenesis, we show that its loss results in increased body weight and organ size without changes of the body proportions. We demonstrate that ohgt knockdown in the fat body, an organ analogous to mammalian liver and adipose tissue, phenocopies the growth phenotypes. We further show that overgrowth is due to an elevation of insulin signaling in ohgt mutants and to the down-regulation of inhibitory cofactors of circulating Drosophila insulin-like peptides (DILPs), named acid-labile subunit and imaginal morphogenesis protein-late 2. The two inhibitory proteins were previously shown to be components of a heterotrimeric complex with growth-promoting DILP2 and DILP5. Our study reveals OHGT as a novel regulator of insulin-dependent organismic growth in Drosophila.

Nuclear cereblon modulates transcriptional activity of Ikaros and regulates its downstream target, enkephalin, in human neuroblastoma cells

Wada, Takeyoshi; Asahi, Toru; Asahi, Toru; Sawamura, Naoya; Sawamura, Naoya

Biochemical and Biophysical Research Communications477(3)p.388 - 3942016年08月-2016年08月　

ISSN：0006291X

概要：© 2016 Elsevier Inc. The gene coding cereblon (CRBN) was originally identified in genetic linkage analysis of mild autosomal recessive nonsyndromic intellectual disability. CRBN has broad localization in both the cytoplasm and nucleus. However, the significance of nuclear CRBN remains unknown. In the present study, we aimed to elucidate the role of CRBN in the nucleus. First, we generated a series of CRBN deletion mutants and determined the regions responsible for the nuclear localization. Only CRBN protein lacking the N-terminal region was localized outside of the nucleus, suggesting that the N-terminal region is important for its nuclear localization. CRBN was also identified as a thalidomide-binding protein and component of the cullin-4-containing E3 ubiquitin ligase complex. Thalidomide has been reported to be involved in the regulation of the transcription factor Ikaros by CRBN-mediated degradation. To investigate the nuclear functions of CRBN, we performed co-immunoprecipitation experiments and evaluated the binding of CRBN to Ikaros. As a result, we found that CRBN was associated with Ikaros protein, and the N-terminal region of CRBN was required for Ikaros binding. In luciferase reporter gene experiments, CRBN modulated transcriptional activity of Ikaros. Furthermore, we found that CRBN modulated Ikaros-mediated transcriptional repression of the proenkephalin gene by binding to its promoter region. These results suggest that CRBN binds to Ikaros via its N-terminal region and regulates transcriptional activities of Ikaros and its downstream target, enkephalin.

Mitochondrial cereblon functions as a Lon-type protease

Kataoka, Kosuke; Nakamura, China; Asahi, Toru; Asahi, Toru; Sawamura, Naoya; Sawamura, Naoya

Scientific Reports62016年07月-2016年07月　

概要：Lon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix, and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. However, whether CRBN has Lon protease-like function remains unknown. Here, we determined if CRBN has a protective function against oxidative stress, similar to Lon protease. We report that CRBN partially distributes in mitochondria, suggesting it has a mitochondrial function. To specify the mitochondrial role of CRBN, we mitochondrially expressed CRBN in human neuroblastoma SH-SY5Y cells. The resulting stable SH-SY5Y cell line showed no apparent effect on the mitochondrial functions of fusion, fission, and membrane potential. However, mitochondrially expressed CRBN exhibited protease activity, and was induced by oxidative stress. In addition, stably expressed cells exhibited suppressed neuronal cell death induced by hydrogen peroxide. These results suggest that CRBN functions specifically as a Lon-type protease in mitochondria.

Mechanical Motion of Chiral Azobenzene Crystals with Twisting upon Photoirradiation

Taniguchi, Takuya; Fujisawa, Juri; Shiro, Motoo; Koshima, Hideko; Asahi, Toru; Asahi, Toru

Chemistry - A European Journal22(23)p.7950 - 79582016年06月-2016年06月　

ISSN：09476539

概要：© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.The photomechanical motion of chiral crystals of trans-azobenzene derivatives with an (S)- and (R)-phenylethylamide group was investigated and compared with a racemic crystal. Changes in the UV/Vis absorption spectra of the powdered crystals before and after UV irradiation were measured by using an optical waveguide spectrometer, showing that the lifetime of the cis-to-trans thermal back-isomerization of the chiral crystals was faster than that of the racemic crystals. Upon UV irradiation, a long plate-like chiral microcrystal bent away from the light source with a twisting motion. A square-like chiral microcrystal curled toward the light with some twisting. Reversible bending of a rod-like chiral microcrystal was repeatable over twenty-five cycles. In contrast, bending of a plate-like racemic microcrystal was small. A possible mechanism for the bending and twisting motion was discussed based on the optimized cis conformer determined by using calculations, showing that the bending motion with twisting is caused by elongation along the b axis and shrinkage along the a axis. Twist and bent: A plate-like chiral azobenzene crystal bent backward with twisting upon UV irradiation from the front. UV irradiation from the rear bent forward the crystal with twisting (see figure).

Interfacial effects on the crystallization and surface properties of poly(l-lactic acid) ultrathin films

Udagawa, Akihiro; Fujie, Toshinori; Kawamoto, Yuko; Saito, Akihiro; Takeoka, Shinji; Asahi, Toru; Asahi, Toru

Polymer Journal48(2)p.157 - 1612016年02月-2016年02月　

ISSN：00323896

概要：© 2016 The Society of Polymer Science, Japan (SPSJ).Polymer crystallization affects structural and mechanical properties of polymer thin films. In this study, we focused on the thermal annealing-induced crystallization in semi-crystalline poly(l-lactic acid) (PLLA) ultrathin films (referred as nanosheets) was investigated in terms of interfacial interaction of PLLA with air and substrate. The surface structure of the PLLA nanosheets observed by atomic force microscopy showed that roughness of the air-side surface increased due to crystallization of PLLA under thermal annealing, whereas that of the substrate-side surface changed little. The elastic moduli and the physical adhesiveness of the nanosheets also changed only on the surface of the air side from crystallization, in contrast to the substrate side. The X-ray diffraction studies of the PLLA nanosheets with different thickness showed that the crystalline contents steeply increased below ca. 200 nm. These results indicated that the crystallization was enhanced near the surface of the air side and restricted near that of the substrate side due to the different interfacial association of the polymer chains in the nanosheet.

Absolute structure determination of chiral crystals consisting of achiral benzophenone with single-crystal X-ray Diffraction and its correlation with solid-state circular dichroism

Matsumoto, Arimasa; Tsuchiya, Sumeru; Hagiwara, Yuki; Ishikawa, Kazuhiko; Koshima, Hideko; Asahi, Toru; Asahi, Toru; Soai, Kenso

Chemistry Letters45(5)p.526 - 5282016年01月-2016年01月　

ISSN：03667022

概要：© 2016 The Chemical Society of Japan.Achiral benzophenone is one of the simplest organic aromatic ketones that crystallize in the chiral form. The absolute structure of chiral crystals consisting of achiral benzophenone was firstly determined with single-crystal X-ray diffraction analysis and the absolute structure was successfully correlated with the solid-state circular dichroism spectra.

Mechanical motion of molecular crystals induced by [4 + 4] photodimerisation

Koshima, Hideko; Uchimoto, Hidetaka; Taniguchi, Takuya; Nakamura, Jun; Asahi, Tsuyoshi; Asahi, Toru; Asahi, Toru

CrystEngComm18(38)p.7305 - 73102016年01月-2016年01月　

概要：© 2016 The Royal Society of Chemistry.The photomechanical bending of crystals of a stilbene-type compound substituted with anthracene and indanone groups, (E)-2-(9-anthrylmethylene)-1-indanone (trans-1), was investigated. When a narrow plate-like microcrystal was irradiated with ultraviolet (UV) light at 365 nm, the crystal gradually bent away from the light source and finally reached a semicircular shape after more than 10 min. A larger rod-like crystal, approximately 10 mm in length, also exhibited a slight bending motion. The cessation of UV irradiation caused the bent crystals to return very slowly to their straight form. In the crystals, the anthracene planes of two neighbouring trans-1 molecules are arranged in a head-to-tail parallel manner, with a short plane-to-plane distance of only 3.72 Å. The 1H nuclear magnetic resonance spectra of trans-1 crystals before and after UV irradiation revealed the intermolecular [4 + 4] photodimerisation of the two anthracene planes, while the trans-to-cis photoisomerisation was not significant. The UV-vis absorption spectra of the trans-1 powder crystals, obtained using a diffuse reflectance spectrophotometer, showed a gradual increase in absorbance between 200 and 500 nm with increasing UV irradiation time, reaching a maximum after 1 h. Thermal back-monomerisation was very slow in the dark, not recovering the initial spectrum even after 25 days. The fluorescence spectra at 570 nm, derived from the anthracene excimer, decreased in intensity with increasing UV irradiation time due to a decrease in the amount of anthracene chromophore via photodimerisation. In situ X-ray measurements revealed that the bending of the crystals was caused by slight elongation of the b axis of the unit cell, corresponding to the long axis of the rod-like crystals. Calculations revealed that the observed crystal elongation could be explained by an optimised head-to-tail [4 + 4] orientation of the anthracene dimer.

Optical Activity and Optical Anisotropy in Photomechanical Crystals of Chiral Salicylidenephenylethylamines

Takanabe, Akifumi;Tanaka, Masahito;Johmoto, Kohei;Uekusa, Hidehiro;Mori, Tadashi;Koshima, Hideko;Asahi, Toru

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY138(45)p.15066 - 150772016年-2016年

ISSN：0002-7863

Ohgata, the Single Drosophila Ortholog of Human Cereblon, Regulates Insulin Signaling-dependent Organismic Growth

Wakabayashi, Satoru;Sawamura, Naoya;Voelzmann, Andre;Broemer, Meike;Asahi, Toru;Hoch, Michael

JOURNAL OF BIOLOGICAL CHEMISTRY291(48)p.25120 - 251322016年-2016年

ISSN：0021-9258

Microwave Effect on Fischer Esterification

Koshima Hideko;Miyazaki Kiminori;Ishii Saori;Asahi Toru

Chemistry Letters0(0)2016年-2016年

ISSN：0366-7022

概要：Upon microwave irradiation, esterification of octanoic acid with 1-octanol in the presence of hydrochloric acid as an acid catalyst proceeded more efficiently in glass vessels than in silicon carbide (SiC) vessels, affording 1-octyl octanoate at higher yields and revealing microwave effects during esterification.

Absolute Structure Determination of Chiral Crystals Consisting of Achiral Benzophenone with Single-crystal X-ray Diffraction and Its Correlation with Solid-state Circular Dichroism

Matsumoto Arimasa;Tsuchiya Sumeru;Hagiwara Yuki;Ishikawa Kazuhiko;Koshima Hideko;Asahi Toru;Soai Kenso

Chemistry Letters45(5)p.526 - 5282016年-2016年

ISSN：0366-7022

概要：Achiral benzophenone is one of the simplest organic aromatic ketones that crystallize in the chiral form. The absolute structure of chiral crystals consisting of achiral benzophenone was firstly determined with single-crystal X-ray diffraction analysis and the absolute structure was successfully correlated with the solid-state circular dichroism spectra.

Microwave Effect on Fischer Esterification

Koshima Hideko;Miyazaki Kiminori;Ishii Saori;Asahi Toru

Chemistry Letters45(5)p.505 - 5072016年-2016年

ISSN：0366-7022

概要：Upon microwave irradiation, esterification of octanoic acid with 1-octanol in the presence of hydrochloric acid as an acid catalyst proceeded more efficiently in glass vessels than in silicon carbide (SiC) vessels, affording 1-octyl octanoate in higher yields and revealing microwave effects during esterification.

18pPSA-61 G-HAUPによる弗化セリウム結晶の磁気光学的研究

中川鉄馬;ビジョラガルシア;島村清史;朝日透

日本物理学会講演概要集70(2)2015年09月-2015年09月　

ISSN：2189079X

19aPS-14 銅酸化物高温超伝導体Bi_2Sr_2CaCu_2O_<8+x>の光学的性質と対称性の破れ

松本匡貴;中川鉄馬;綿打敏司;松田梓;朝日透

日本物理学会講演概要集70(2)2015年09月-2015年09月　

ISSN：2189079X

Cereblon is recruited to aggresome and shows cytoprotective effect against ubiquitin-proteasome system dysfunction

Sawamura, Naoya; Sawamura, Naoya; Wakabayashi, Satoru; Matsumoto, Kodai; Yamada, Haruka; Asahi, Toru; Asahi, Toru

Biochemical and Biophysical Research Communications464(4)p.1054 - 10592015年09月-2015年09月　

ISSN：0006291X

概要：© 2015 Elsevier Inc. All rights reserved. Cereblon (CRBN) is encoded by a candidate gene for autosomal recessive nonsyndromic intellectual disability (ID). The nonsense mutation, R419X, causes deletion of 24 amino acids at the C-terminus of CRBN, leading to mild ID. Although abnormal CRBN function may be associated with ID disease onset, its cellular mechanism is still unclear. Here, we examine the role of CRBN in aggresome formation and cytoprotection. In the presence of a proteasome inhibitor, exogenous CRBN formed perinuclear inclusions and co-localized with aggresome markers. Endogenous CRBN also formed perinuclear inclusions under the same condition. Treatment with a microtubule destabilizer or an inhibitor of the E3 ubiquitin ligase activity of CRBN blocked formation of CRBN inclusions. Biochemical analysis showed CRBN containing inclusions were high-molecular weight, ubiquitin-positive. CRBN overexpression in cultured cells suppressed cell death induced by proteasome inhibitor. Furthermore, knockdown of endogenous CRBN in cultured cells increased cell death induced by proteasome inhibitor, compared with control cells. Our results show CRBN is recruited to aggresome and has functional roles in cytoprotection against ubiquitin-proteasome system impaired condition.

Model of complex chiral drug metabolic systems and numerical simulation of the remaining chirality toward analysis of dynamical pharmacological activity

Ogino, Yoshiyuki; Asahi, Toru

Journal of Theoretical Biology373p.117 - 1312015年05月-2015年05月　

ISSN：00225193

概要：© 2015 Elsevier Ltd. In this study, systems of complicated pathways involved in chiral drug metabolism were investigated. The development of chiral drugs resulted in significant improvement in the remedies available for the treatment of various severe sicknesses. Enantiopure drugs undergo various biological transformations that involve chiral inversion and thus result in the generation of multiple enantiomeric metabolites. Identification of the specific active substances determining a given drug[U+05F3]s efficacy among such a mixture of different metabolites remains a challenge. To comprehend this complexity, we constructed a mathematical model representing the complicated metabolic pathways simultaneously involving chiral inversion. Moreover, this model is applied to the metabolism of thalidomide, which has recently been revived as a potentially effective prescription drug for a number of intractable diseases. The numerical simulation results indicate that retained chirality in the metabolites reflects the original chirality of the unmetabolized drug, and a higher level of enantiomeric purity is preserved during spontaneous degradation. In addition, chirality remaining after equilibration is directly related to the rate constant not only for chiral inversion but also for generation and degradation. Furthermore, the retention of chirality is quantitatively predictable using this combination of kinetic parameters. Our simulation results well explain the behavior of thalidomide in the practical biological experimental data. Therefore, this model promises a comprehensive understanding of dynamic metabolic systems involving chiral drugs that express multiple enantiospecific drug efficacies.

Self-disproportionation of enantiomers of thalidomide and its fluorinated analogue via gravity-driven achiral chromatography: mechanistic rationale and implications

Maeno, Mayaka;Tokunaga, Etsuko;Yamamoto, Takeshi;Suzuki, Toshiya;Ogino, Yoshiyuki;Ito, Emi;Shiro, Motoo;Asahi, Toru;Shibata, Norio

CHEMICAL SCIENCE6(2)p.1043 - 10482015年-2015年

ISSN：2041-6520

Model of complex chiral drug metabolic systems and numerical simulation of the remaining chirality toward analysis of dynamical pharmacological activity

Ogino, Yoshiyuki;Asahi, Toru

JOURNAL OF THEORETICAL BIOLOGY373p.117 - 1312015年-2015年

ISSN：0022-5193

Cereblon is recruited to aggresome and shows cytoprotective effect against ubiquitin-proteasome system dysfunction

Sawamura, Naoya;Wakabayashi, Satoru;Matsumoto, Kodai;Yamada, Haruka;Asahi, Toru

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS464(4)p.1054 - 10592015年-2015年

ISSN：0006-291X

Crystal structure of (S)-4-carbamoyl-4-(1,3-dioxo-isoindolin-2-yl)butanoic acid.

Otogawa Kohei;Ishikawa Kazuhiko;Shiro Motoo;Asahi Toru

Acta crystallographica. Section E, Crystallographic communications71(Pt 1)2015年-2015年

概要：:In the title compound, C13H12N2O5, the phthalimide ring system is essentially planar, with a maximum deviation of 0.0479 (14) Å. In the crystal, each mol-ecule is linked via six neighbouring mol-ecules into a three-dimensional network through N-H⋯O and O-H⋯O hydrogen bonds, which form an R 3 (2)(8) ring motif.

Model of complex chiral drug metabolic systems and numerical simulation of the remaining chirality toward analysis of dynamical pharmacological activity.

Ogino Yoshiyuki;Asahi Toru

Journal of theoretical biology3732015年-2015年

ISSN：1095-8541

概要：:In this study, systems of complicated pathways involved in chiral drug metabolism were investigated. The development of chiral drugs resulted in significant improvement in the remedies available for the treatment of various severe sicknesses. Enantiopure drugs undergo various biological transformations that involve chiral inversion and thus result in the generation of multiple enantiomeric metabolites. Identification of the specific active substances determining a given drug׳s efficacy among such a mixture of different metabolites remains a challenge. To comprehend this complexity, we constructed a mathematical model representing the complicated metabolic pathways simultaneously involving chiral inversion. Moreover, this model is applied to the metabolism of thalidomide, which has recently been revived as a potentially effective prescription drug for a number of intractable diseases. The numerical simulation results indicate that retained chirality in the metabolites reflects the original chirality of the unmetabolized drug, and a higher level of enantiomeric purity is preserved during spontaneous degradation. In addition, chirality remaining after equilibration is directly related to the rate constant not only for chiral inversion but also for generation and degradation. Furthermore, the retention of chirality is quantitatively predictable using this combination of kinetic parameters. Our simulation results well explain the behavior of thalidomide in the practical biological experimental data. Therefore, this model promises a comprehensive understanding of dynamic metabolic systems involving chiral drugs that express multiple enantiospecific drug efficacies.

Cereblon is recruited to aggresome and shows cytoprotective effect against ubiquitin-proteasome system dysfunction.

Sawamura Naoya;Wakabayashi Satoru;Matsumoto Kodai;Yamada Haruka;Asahi Toru

Biochemical and biophysical research communications464(4)2015年-2015年

ISSN：1090-2104

概要：:Cereblon (CRBN) is encoded by a candidate gene for autosomal recessive nonsyndromic intellectual disability (ID). The nonsense mutation, R419X, causes deletion of 24 amino acids at the C-terminus of CRBN, leading to mild ID. Although abnormal CRBN function may be associated with ID disease onset, its cellular mechanism is still unclear. Here, we examine the role of CRBN in aggresome formation and cytoprotection. In the presence of a proteasome inhibitor, exogenous CRBN formed perinuclear inclusions and co-localized with aggresome markers. Endogenous CRBN also formed perinuclear inclusions under the same condition. Treatment with a microtubule destabilizer or an inhibitor of the E3 ubiquitin ligase activity of CRBN blocked formation of CRBN inclusions. Biochemical analysis showed CRBN containing inclusions were high-molecular weight, ubiquitin-positive. CRBN overexpression in cultured cells suppressed cell death induced by proteasome inhibitor. Furthermore, knockdown of endogenous CRBN in cultured cells increased cell death induced by proteasome inhibitor, compared with control cells. Our results show CRBN is recruited to aggresome and has functional roles in cytoprotection against ubiquitin-proteasome system impaired condition.

Arctic mutatn Aβ40 aggregates on α7 nicotinic acetylcholine receptors and inhibits their functions

Ye Ju, Toru Asahi and Naoya Sawamura

Journal of Neurochemistry2014年08月-

Quantitative analysis with advanced compensated polarized light microscopy on wavelength dependence of linear birefringence of single crystals causing arthritis

Akifumi Takanabe, Masahito Tanaka, Atsuo Taniguchi, Hisashi Yamanaka and Toru Asahi

Journal of Physics D: Applied Physics47(28)p.2854022014年06月-

A label-free electrical assay of fibrous amyloid β based on semiconductor biosensing

Sho Hideshima, Masumi Kobayashi, Takeyoshi Wada, Shigeki Kuroiwa, Takuya Nakanishi, Naoya Sawamura, Toru Asahi and Tetsuya Osaka

chemical communications50p.3476 - 34792014年02月-

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes

Nakagawa, Kenta;Harper-Lovelady, Heather;Tanaka, Yuji;Tanaka, Masahito;Yamato, Masayuki;Asahi, Toru

CHEMICAL COMMUNICATIONS50(95)p.15086 - 150892014年-2014年

ISSN：1359-7345

A label-free electrical assay of fibrous amyloid beta based on semiconductor biosensing

Hideshima, Sho;Kobayashi, Masumi;Wada, Takeyoshi;Kuroiwa, Shigeki;Nakanishi, Takuya;Sawamura, Naoya;Asahi, Toru;Osaka, Tetsuya

CHEMICAL COMMUNICATIONS50(26)p.3476 - 34792014年-2014年

ISSN：1359-7345

Quantitative analysis with advanced compensated polarized light microscopy on wavelength dependence of linear birefringence of single crystals causing arthritis

Takanabe, Akifumi;Tanaka, Masahito;Taniguchi, Atsuo;Yamanaka, Hisashi;Asahi, Toru

JOURNAL OF PHYSICS D-APPLIED PHYSICS47(28)2014年-2014年

ISSN：0022-3727

Arctic mutant A beta 40 aggregates on alpha 7 nicotinic acetylcholine receptors and inhibits their functions

Ju, Ye;Asahi, Toru;Sawamura, Naoya

JOURNAL OF NEUROCHEMISTRY131(5)p.667 - 6742014年-2014年

ISSN：0022-3042

A label-free electrical assay of fibrous amyloid β based on semiconductor biosensing.

Hideshima Sho;Kobayashi Masumi;Wada Takeyoshi;Kuroiwa Shigeki;Nakanishi Takuya;Sawamura Naoya;Asahi Toru;Osaka Tetsuya

Chemical communications (Cambridge, England)50(26)2014年-2014年

ISSN：1364-548X

概要：:We propose, as an alternative to conventional spectroscopic assays, a simple method for discriminating fibrous amyloid proteins by using a label-free semiconductor-based biosensor. The highly sensitive assay is expected to be useful for accelerating amyloid related research.

Arctic mutant Aβ40 aggregates on α7 nicotinic acetylcholine receptors and inhibits their functions.

Ju Ye;Asahi Toru;Sawamura Naoya

Journal of neurochemistry131(5)2014年-2014年

ISSN：1471-4159

概要：:Amyloid β protein (Aβ) plays a central role in the pathogenesis of Alzheimer's disease (AD). Point mutations within the Aβ sequence associated with familial AD (FAD) are clustered around the central hydrophobic core of Aβ. Several types of mutations within the Aβ sequence have been identified, and the 'Arctic' mutation (E22G) has a purely cognitive phenotype typical of AD. Previous studies have shown that the primary result of the 'Arctic' mutation is increased formation of Aβ protofibrils. However, the molecular mechanism underlying this effect remains unknown. Aβ42 binds to a neuronal nicotinic acetylcholine receptor subunit, neuronal acetylcholine receptor subunit alpha-7 (CHRNA7), with high affinity and, thus, may be involved in the pathogenesis of AD. Therefore, to clarify the molecular mechanism of Arctic mutation-mediated FAD, we focused on CHRNA7 as a target molecule of Arctic Aβ. We performed an in vitro binding assay using purified CHRNA7 and synthetic Arctic Aβ40, and demonstrated that Arctic Aβ40 specifically bound to CHRNA7. The aggregation of Arctic Aβ40 was enhanced with the addition of CHRNA7. Furthermore, the function of CHRNA7 was detected by measuring Ca(2+) flux and phospho-p44/42 MAPK (ERK1/2) activation. Our results indicated that Arctic Aβ40 aggregation was enhanced by the addition of CHRNA7, which destabilized the function of CHRNA7 via inhibition of Ca(2+) responses and activation of ERK1/2. These findings indicate that Arctic Aβ mutation may be involved in the mechanism underlying FAD. This mechanism may involve binding and aggregation, leading to the inhibition of CHRNA7 functions.

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes.

Nakagawa Kenta;Harper-Lovelady Heather;Tanaka Yuji;Tanaka Masahito;Yamato Masayuki;Asahi Toru

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes.50(95)2014年-2014年

ISSN：1364-548X

概要：:A novel method for fabricating anisotropic collagen membranes, which regulate the preferred orientation, is developed. The high-accuracy universal polarimeter (HAUP) method is used for simultaneous and quantitative evaluations of the optical anisotropy and optical activity. The evaluation of these optical quantities, enabled by the HAUP method, is beneficial to the field of tissue engineering.

理工系ポスドク・博士人材のキャリアデザインと大学院改革(<シリーズ>"ポスドク"問題その28)

朝日透;中里弘道;鈴木清貴

日本物理學會誌68(9)p.623 - 6272013年09月-2013年09月　

ISSN：00290181

Metastable Liquid Crystal as Time-Responsive Reaction Medium: Aging-Induced Dual Enantioselective Control

Y.Ishida, Y.Matsunaka, Y.Kai, K.Yamada. K.Nakagawa, T.Asahi, K.Saigo

Journal of the American Chemical Society135(17)p.6407 - 64102013年05月-

Construction of photoenergetic mitochondria in cultured mammalian cells

Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura

Scientific Reports3(1635)2013年04月-

Selective Molecular Permeability Induced by Glass Transition Dynamics of Semicrystalline Polymer Ultrathin Films

Toshinori Fujie, Yuko Kawamoto, Hiroki Haniuda, Akihiro Saito, Koki Kabata, Yukio Honda, Eriko Ohmori, Toru Asahi, Shinji Takeoka

Macromolecules46(2)p.395 - 4022013年01月-

Selective Molecular Permeability Induced by Glass Transition Dynamics of Semicrystalline Polymer Ultrathin Films

Fujie, Toshinori;Kawamoto, Yuko;Haniuda, Hiroki;Saito, Akihiro;Kabata, Koki;Honda, Yukio;Ohmori, Eriko;Asahi, Toru;Takeoka, Shinji

MACROMOLECULES46(2)p.395 - 4022013年-2013年

ISSN：0024-9297

Construction of photoenergetic mitochondria in cultured mammalian cells

Hara, Kiyotaka Y.;Wada, Takeyoshi;Kino, Kuniki;Asahi, Toru;Sawamura, Naoya

SCIENTIFIC REPORTS32013年-2013年

ISSN：2045-2322

Metastable Liquid Crystal as Time-Responsive Reaction Medium: Aging-Induced Dual Enantioselective Control

Ishida, Yasuhiro;Matsuoka, Yuki;Kai, Yukiko;Yamada, Kuniyo;Nakagawa, Kenta;Asahi, Toru;Saigo, Kazuhiko

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY135(17)p.6407 - 64102013年-2013年

ISSN：0002-7863

Generalized High-Accuracy Universal Polarimeter (G-HAUP) and Its Application

T. Asahi, M. Tanaka

Polymers (The Society of Polymer Science, Japan)Vol.6p.777 - 7782012年10月-

Generalized High Accuracy Universal Polarimeter の測定法とその応用

朝日透;田中真人

高分子61(10)p.777 - 7782012年10月-2012年10月　

ISSN：04541138

Absolute chirality of the γ-polymorph of glycine: correlation of the absolute structure with the optical rotation

Kazuhiko Ishikawa, Masahito Tanaka, Toshiya Suzuki, Akiko Sekine, Tsuneomi Kawasaki, Kenso Soai, Motoo Shiro, Meir Lehav and Toru Asahi

Chemical Communications, Communication2012年04月-

An application of the advanced high-accuracy universal polarimeter to the chiroptical measurement of an intercalated compound K4Nb6O17 with high anisotrophy

Masahito Tanaka, Naomichi Nakamura, Hideo Koshima, Toru Asahi

Journal of physics D: Applied physics45p.1 - 72012年03月-

An application of the advanced high-accuracy universal polarimeter to the chiroptical measurement of an intercalated compound K4Nb6O17 with high anisotropy

Tanaka, Masahito;Nakamura, Naomichi;Koshima, Hideko;Asahi, Toru

JOURNAL OF PHYSICS D-APPLIED PHYSICS45(17)2012年-2012年

ISSN：0022-3727

Absolute chirality of the gamma-polymorph of glycine: correlation of the absolute structure with the optical rotation

Ishikawa, Kazuhiko;Tanaka, Masahito;Suzuki, Toshiya;Sekine, Akiko;Kawasaki, Tsuneomi;Soai, Kenso;Shiro, Motoo;Lahav, Meir;Asahi, Toru

CHEMICAL COMMUNICATIONS48(48)p.6031 - 60332012年-2012年

ISSN：1359-7345

Absolute chirality of the γ-polymorph of glycine: correlation of the absolute structure with the optical rotation.

Ishikawa Kazuhiko;Tanaka Masahito;Suzuki Toshiya;Sekine Akiko;Kawasaki Tsuneomi;Soai Kenso;Shiro Motoo;Lahav Meir;Asahi Toru

Absolute chirality of the γ-polymorph of glycine: correlation of the absolute structure with the optical rotation.2012年-2012年

ISSN：1364-548X

概要：:Crystal specimens of the γ-polymorph of achiral glycine which crystallize in space groups P3(1) and P3(2) as determined by the anomalous X-ray scattering method are shown to be laevorotatory and dextrorotatory, respectively, as determined by optical rotation of the crystals.

Transparent, Tough Collagen Laminates Prepared by Oriented Flow Casting, Multi-Cyclic Vitrification and Chemical Cross-Linking

Y. Tanaka, K. Baba, T. J. Duncan, A. Kubota, T. Asahi, A. J. Quantock, M. Yamato, T. Okano, K. Nishida

Biomaterials32p.3358 - 33662011年05月-

Buffers to Suppress SDS Adsorption to PEO for Protein Separation on Capillary Polymer Electrophoresis

K. Sumitomo, K. Mayumi, H. Minamikawa, M. Masuda, T. Asahi, T. Shimizu, K. Ito, Y. Yamaguchi

Electrophoresis32p.448 - 4542011年02月-

Buffers to suppress sodium dodecyl sulfate adsorption to polyethylene oxide for protein separation on capillary polymer electrophoresis

Sumitomo, Keiko;Mayumi, Koichi;Minamikawa, Hiroyuki;Masuda, Mitsutoshi;Asahi, Toru;Shimizu, Toshimi;Ito, Kohzo;Yamaguchi, Yoshinori

ELECTROPHORESIS32(3-4)p.448 - 4542011年-2011年

ISSN：0173-0835

Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking

Tanaka, Yuji;Baba, Koichi;Duncan, Thomas J.;Kubota, Akira;Asahi, Toru;Quantock, Andrew J.;Yamato, Masayuki;Okano, Teruo;Nishida, Kohji

BIOMATERIALS32(13)p.3358 - 33662011年-2011年

ISSN：0142-9612

Improvement of Magnetic Properties and Evaluation of Read/Write Characteristics on Granular SmCo5 Perpendicular Magnetic Thin Films

T. Asahi, I. Koizumi, Y. Egawa, M. Yoshino, A. Sugiyama, J. Hokkyo, T. Kiya, J. Ariake, K. Ouchi, T. Osaka

Journal of Magnetism and Magnetic Materials322p.3784 - 37882010年12月-

Fabrication of SmCo5-CrTa Granular Films

A. Sugiyama, I. Koizumi, Y. Egawa, M. Yoshino, J. Hokkyo, T. Asahi, T. Osaka

IEEE Transaction on Magnetics46p.1699 - 17012010年06月-

Evaluation of Stability Difference between Asymmetric Homochiral Dimer in (S)-Thalidomide Crystal and Symmetric Heterochiral Dimer in (RS)-Thalidomide Crystal

T. Suzuki, M. Tanaka, M. Shiro, N. Shibata, T. Osaka, T. Asahi

Phase Transitions83(3)p.223 - 2342010年03月-

Effects of CdSe/ZnS Quantum Dots Covered Multi-Walled Carbon Nanotubes on Murine Embryonicstem Cells

D. Cui, H. Zhang, J. Sheng, Z. Wang, T. Asahi, R. He, T. Osaka, F. Gao, Hoon Sung Cho, C. Huth, H. Hu, G. M. Pauletti, D. Shi

Nano Biomedicine and Engineering2p.236 - 2442010年-

Evaluation of stability difference between asymmetric homochiral dimer in (S)-thalidomide crystal and symmetric heterochiral dimer in (RS)-thalidomide crystal

Suzuki, Toshiya;Tanaka, Masahito;Shiro, Motoo;Shibata, Norio;Osaka, Tetsuya;Asahi, Toru

PHASE TRANSITIONS83(3)p.223 - 2342010年-2010年

ISSN：0141-1594

Improvement of magnetic intergranular isolation and evaluation of read/write characteristics on SmCo5 perpendicular magnetic thin films

Asahi, Toru;Koizumi, Isao;Egawa, Yuko;Yoshino, Masahiro;Sugiyama, Atsushi;Hokkyo, Jiro;Kiya, Takanori;Ariake, Jun;Ouchi, Kazuhiro;Osaka, Tetsuya

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS322(23)p.3784 - 37882010年-2010年

ISSN：0304-8853

Single Walled Carbon Nanotubes Based Regulation of Proliferation and Differentiation of Mouse Embryonic Stem Cells

D. Cui, Z. Wang, H. Zhang, J. Ruan, C. Bao, H. Yang, T. Asahi, H. Song, K. Wang

ECS Transactions19p.63 - 672009年-

Magnetic Circular Dichroism of [Co/Pd] and [CoB/Pd] Multilayered Films

A. Agui. T. Asahi, J. Sayama, M. Mizumaki, M. Tanaka, T. Osaka

Journal of Magnetism and Magnetic Materials320p.3015 - 30182008年11月-

Improvement of Magnetic Properties and Read/Write Characteristics in SmCo5 Perpendicular Thin Films

T. Asahi, I. Koizumi, Y. Kikuchi, M. Yoshino, A. Sugiyama, J. Hokkyo, T. Osaka

Journal of Magnetisc and Magnetic Materials320p.3075 - 30782008年11月-

Effect of Growth Temperature on the Shape and Crystallinity of Chemically Produced FePt Nanoparticles

T. Hachisu, T. Yotsumoto, A. Sugiyama, H. Iida, T. Nakanishi, T. Asahi, T. Osaka

Chemistry Letters37p.840 - 8412008年08月-

Existence and Origin of Compensation Layer Thickness in Tb20Co80/Pd Multilayered Films

M. Tanaka, T. Asahi, A. Agui, M. Mizumaki, J. Sayama, T. Osaka

Journal of Physics D:Applied Physics41p.55003 - 550062008年-

Self-Assembly of Quantum Dots and Carbon Nanotubes for Ultrasensitive DNA and Antigen Detection

D. Cui, B. Pan, H. Zhang, F. Gao, R. Wu, J. Wang, R. He, T. Asahi

Analytical Chemistry80p.7996 - 80012008年-

Effect of Surface Coverage of Gold(111) Electrode with Cysteine on the Chiral Discrimination of DOPA

M. Matsunaga, T. Nakanishi, T. Asahi, T. Osaka

Chirality19p.S36 - S392007年05月-

Highly Enantioselective Discrimination of Amino Acids using Copper Deposition on a Gold Electrode Modified with Homocysteine Monolayer

M. Matsunaga, T. Nakanishi, T. Asahi, T. Osaka

Electrochmistry Communications9p.725 - 7282007年04月-

Sputter-Deposited SmCo5 Thin Films with Perpendicular Magnetic Anisotropy for High Density Magnetic Recording Media

J. Sayama, Y. Yamashita, T. Asahi, T. Osaka

Magnetic Materials, Processes, and Devices9 ECS Transaction3p.307 - 3132007年-

Enantioselectivity of Redox Reaction of DOPA at the Gold Electrode Modified with a Self-assembly Monolayer of Homocycteine

T. Nakanishi, M. Matsunaga, M. Nagasaka, T. Asahi, T. Osaka

Journal of American Chemical Society128p.13322 - 133232006年10月-

Soft X-ray Absorption Spectroscopy and Magnetic Circular Dichroism Study of Electroless-deposited CoNiFe Ternary Alloy Soft Magnetic Film

M. Tanaka, N. Nakamura, T. Asahi, T. Tsumori, A. Agui, M. Mizumaki, T. Osaka

Physica Status Solidi3p.2783 - 27862006年09月-

Crystalline of Leucine on a self-assembled Monolayer with Covalently Attached Enantiomeric Leucine Molecules

T. Nakanishi, N. Banno, M. Matsunaga, T. Asahi, T. Osaka

Colloids and Surfaces A: Physicochemical and Engineering Aspects284p.270 - 2752006年08月-

Electrochemical Formation of Intermediate Layer for Co/Pd Multilayered Media

J. Kawaji, K. Kimura, T. Asahi, T. Homma, T. Osaka

Journal of Magnetism and Magnetic Materials303p.128 - 1322006年08月-

Study on N2-added and B-doped effect in Co/Pd multilayered films using soft x-ray absorption and magnetic circular dichroism

A. Agui, T. Asahi, J. Sayama, J. Kawaji, M. Mizumaki, M. Tanaka, T. Osaka

Jornal of Physics D: Applied Physics39p.2881 - 28852006年07月-

Origin of Perpendicular Magnetic Anisotropy of SmCo5 Thin Films with Cu Underlayer

J. Sayama, K. Mizutani, T. Asahi, J. Ariake, K. Ouchi, T. Osaka

Journal of Magnetisc and Magnetic Materials301p.271 - 2782006年06月-

Effect of Substrate Morphology on the Crystallization of Leucine on Gold Surface Modified with a Self-assembled Monolayer

T. Osaka, M. Matsunaga, T. Nakanishi, T. Asahi, N. Banno

Chemistry Letters35p.438 - 4392006年04月-

Incident Angle Dependence of MCD at the Dy M5-Edge of Perpendicular Magnetic DyxCo100?x Films

A. Agui, M. Mizumaki, T. Asahi, J. Sayama, K. Matsumoto, T. Morikawa, T. Matsushita, T. Osaka, Y. Miura

Journal of Alloys and Compounds408p.741 - 7452006年02月-

Magnetic Properties and Domain Structure of SmCo5 Perpendicular Magnetization Films Prepared by Using a UHV Sputtering System

J. Sayama, T. Asahi, Y. Yamashita, J. Ariake, K. Ouchi, T. Osaka

Journal of the Magnetics Society of Japan30p.423 - 4282006年-

SmCo5 Thin Films with High Magnetic Anisotropy for Perpendicular Magnetic Recording

J. Sayama, K. Mizutani, Y. Yamashita, T. Asahi, T. Osaka

IEEE Transactions on Magnetics41p.3133 - 31352005年10月-

Optical Activity Induced by Helical Arrangements of Tryptamine and 4-Chlorobenzoic Acid in Their Cocrystal

H. Koshima, M. Nagano, T. Asahi

Journal of American Chemical Society127p.2455 - 24632005年04月-

Effect of N2 Additive Gas during Sputtering on Magnetic Properties and Microstructure of CoB/Pd Multilayered Media

T. Asahi, M. Tanaka, J. Sayama, J. Kawaji, S. Matsunuma, T. Osaka

Journal of Magnetisc and Magnetic Materials287p.199 - 2032005年02月-

Electroless-Deposited Soft Magnetic Underlayer on Silicon Disk Substrate for Double-Layered Perpendicular Magnetic Recording Media

T. Osaka, T. Asahi, T. Yokoshima, J. Kawaji

Journal of Magnetism and Magnetic Materials287p.292 - 2972005年02月-

Magnetic Properties and Microstructure of SmCo5 Thin Film with Perpendicular Magnetic Anisotropy

J. Sayama, K. Mizutani, T. Asahi, J. Ariake, K. Ouchi, S. Matsunuma, T. Osaka

Journal of Magnetism and Magnetic Materials287p.239 - 2442005年02月-

Microstructure of a Co/Pd Multilayered Perpendicular Recording Medium with Pd Seeds Prepared by Electrochemical Process

M. Tanaka, J. Kawaji, K. Kimura, T. Asahi, T. Homma, S. Matsunuma, T. Osaka

Journal of Magnetism and Magnetic Materials287p.188 - 1922005年02月-

Electroless Deposition of Soft Magnetic Underlayer for a Double-layered Perpendicular Recording Media

A. Sugiyama, T. Asahi, K. Adachi, M. Sakagami, J. Kawaji, M. Yoshino, T. Osaka, T. Tsumori

IEICE Technical ReportMR2004-48p.15 - 202005年-

Application of Soft X-ray Magnetic Circular Dichroism Spectroscopy to the Investigation of [Co/Pd] Multilayered Perpendicular Magnetic Recording Media with Various Seedlayers

A. Agui, M. Mizumaki, T. Matsushita, T. Asahi, J. Kawaji, J. Sayama, T. Osaka

Journal of Applied Physics95p.7825 - 78312004年06月-

Chiral Discrimination Between Thalidomide Enantiomers Using a Solid Surface With Two-Dimensional Chirality

T. Nakanishi, N. Yamakawa, T. Asahi, N. Shibata, B. Ohtani, T. Osaka

Chirality16p.S36 - S392004年06月-

Enantioselective Crystal Growth of Leucine on a Self-Assembled Monolayer with Covalently Attached Leucine Monolayers

N. Banno, T. Nakanishi, M. Matsunaga, T. Asahi, T. Osaka

Journal of American Chemical Society126p.428 - 4292004年-

Formation of Pd Nanocluster Seed by Electrochemical Process for Control of Magnetic Properties in Co/Pd Multilayered Film

J. Kawaji, M. Tanaka, K. Kimura, T. Asahi, T. Homma, T. Osaka

IEEE Transactions on Magnetics40p.2473 - 24752004年-

Magnetic Properties of SmCo5 Perpendicular. Magnetization Film with Cu Seedlayer

J. Sayama, K. Mizutani, T. Asahi, T. Osaka

IEICE Technical ReportMR2004-8p.7 - 112004年-

Microstructure and Magnetic Properties of a Co/Pd Multilayer on a Controlled Pd/si Seedlayer for Double-Layered Perpendicular Magnetic Recording media

J. Kawaji, T. Asahi, H. Hashimoto, J. Hokkyo, S. Matsunuma, G. Safran, J. Ariake, K. Ouchi, T. Osaka

Journal of Applied Physics95p.8023 - 80292004年-

Newly developed SmCo5 thin film with perpendicular magnetic anisotropy

J. Sayama, T. Asahi, K. Mizutani, T. Osaka

Journal of Physics D: Applied Physics37p.1 - 42004年-

Soft x-ray Magnetic Circular Dichroism Study of [Co/Pd] Multilayered Perpendicular Magnetic Films

A. Agui, M. Mizumaki, T. Matsushita, T. Asahi, J. Kawaji, J. Sayama, T. Osaka

Journal of Applied Physics95p.7825 - 78312004年-

Thin films of SmCo5 with very high perpendicular magnetic anisotropy

J. Sayama, K. Mizutani, T. Asahi, T. Osaka

Applied Physics Letters85p.5640 - 56422004年-

MCD Measurement at the Tb M4,5-edges of Tb17FexCo(83-x) Perpendicular Magnetization Films

A. Agui, M. Mizumaki, T. Asahi, J. Sayama, K. Matsumoto, T. Morikawa, T. Nakatani, T. Matsushita, T. Osaka, Y. Miura

Transactions of the Magnetics Society of Japan4p.326 - 3292004年-

Optimization of the Thickness Ratio of Pd/Si Intermediate Layer in a Co/Pd Multilayer Perpendicular Magnetic Recording Media

J. Kawaji, H. Hashimoto, T. Asahi, J. Hokkyo, T. Osaka

Transactions of the Magnetics Society of Japan4p.78 - 842004年-

Measurement of Circular Dichroism of Ferroelectric Fresnoite Ba2Si2TiO8

T. Asahi, T. Osaka, J. Kobayashi

Proceedings of SPIE5218p.223 - 2322003年-

Enantioselective Adsorption of Phenylalanine onto Self-Assembled Monolayers of 1, 1’-Binaphthalene-2, 2’-dithiol on Gold

T. Nakanishi, N. Yamakawa, T. Asahi, T. Osaka, B. Ohtani, K. Uosaki

Journal of American Chemical Society124p.740 - 7412002年-

Enantioselective adsorption of phenylalanine onto self-assembled monolayers of 1,1 '-binaphthalene-2,2 '-dithiol on gold

T. Nakanishi, N. Yamakawa, T. Asahi, T. Osaka, B. Ohtani, K. Uosaki

Journal of American Chemical Society124p.740 - 7412002年-

Enhancement of Magnetic Properties of Co/Pd Multilayered Perpendicular Magnetic Recording Media by Using Pd/Si Dual Seedlayer

J. Kawaji, T. Asahi, T. Onoue, J. Sayama, J. Hokkyo, T. Osaka, K. Ouchi

Journal of Magnetism and Magnetic Materials251p.220 - 2282002年-

Microstructure and Magnetic Properties of Co/Pd Multilayered Thin Films with C or Si Seedlayer

T. Onoue, T. Asahi, K. Kuramochi, J. Kawaji, T. Osaka, J. Ariake, K. Ouchi, G. Safran, N. Yaguchi

Journal of Applied Physics92p.4545 - 45522002年-

Thermal Deposition High-Resolution Mass Spectroscopy of mixed Self-Assmbled Monolayers on Gold

T. Shibue, T. Nakanishi, T. Matsuda, T. Asahi, T. Osaka

Langmuir18p.1528 - 15342002年-

Absolute Configuration of L-(+)589-Tris(Ethylenediamine) Cobalt(III) Triiodide Monohydrate

R. Matsuki, T. Asahi, H. Asai, M. Shiro

Acta Crystallographica E57p.1 - 32001年-

Analysis of Microstructure for Co/Pd Multilayer Perpendicular Magnetic Recording Media with Carbon

T. Asahi, K. Kuramochi, J. Kawaji, T. Onoue, T. Osaka

Journal of Magnetism and Magnetic Materials235p.87 - 922001年-

CoCrPtTa and Co/Pd Perpendicular Magnetic Recording Media with Amorphous Underlayers

T. Onoue, T. Asahi, K. Kuramochi, J. Kawaji, T. Homma, T. Osaka

IEEE Transaction on Magnetics37p.1592 - 15942001年-

Effect of Underlayers on Magnetic Properties of a Co/Pd Multilayer Perpendicular Magnetic Recording Medium

T. Onoue, J. Kawaji, K. Kuramochi, T. Asahi, T. Osaka

Journal of Magnetism and Magnetic Materials235p.82 - 862001年-

Enantioselective Fluorination Mediated by Cinchona Alkaloid Derivatives / Selectfluor Combinations: Reaction Scope and Structural Information of N-Fluorocinchona Alkaloids

N. Shibata, E. Suzuki, T. Asahi, M. Shiro

Journal of American Chemical Society123p.7001 - 70092001年-

Improvement of Signal to Noise Ratio for Co/Pd Multilayer Perpendicular Magnetic Recording Media by the Addition of an Underlayer

T. Onoue, T. Asahi, K. Kuramochi, J. Kawaji, T. Homma, T. Osaka

Journal of Magnetism and Magnetic Materials235p.40 - 442001年-

Optical Study of a Phase Transition of Fresnoite

T. Asahi, T. Osaka, J. Kobayashi, S. C. Abrahams, S. Nanamatsu, M. Kimura

Physical Review B63p.1 - 132001年-

Polarimetry of Electromagnetic Materials

T. Asahi, T. Osaka, J. Kobayashi

Proceedings of SPIE4467p.20 - 302001年-

Relationship Between Surface Energy and Preferred Orientation of CoCr Alloy Magnetic Thin Films Deposited on C and TiCr

H. Okuo, T. Asahi, T. Onoue, T. Osaka

Journal of Magnetism and Magnetic Materials235p.98 - 1032001年-

Analysis for Magnetized State of Perpendicular Magnetic Recording Media Using MFM

M. Kawakami, T. Onoue, T. Asahi, J. Hokkyo, T. Osaka

Journal of the Magnetics Society of Japan25p.571 - 5742001年-

Changes of Microstructure Versus Film Thickness in CoCrPtTa/NiCr Media

K. Kurasawa, M. Kawakami, T. Onoue, T. Asahi, T. Osaka

Journal of the Magnetics Society of Japan25p.623 - 6262001年-

Preparation of Co/Pd Multilayer Perpendicular Magnetic Recording Media with Carbon Under Layer

T. Asahi, K. Kuramochi, J. kawaji, T. Onoue, T. Osaka

Journal of the Magnetics Society of Japan25p.575 - 5782001年-

Preparation of a Perpendicular/Longitudinal Composite Medium with a Silicon Intermediate Layer

Y. Morita, T. Onoue, T. Asahi, T. Honma, T. Osaka

Journal of the Magnetics Society of Japan25p.619 - 6222001年-

Application of Si Amorphous Underlayers to CoCrPtTa Perpendicular Magnetic Recording Media

T. Asahi, T. Onoue, M. Ikeda, Y. Morita, T. Osaka

Ferrites: Proceedings of the 8th International Conference on Ferritep.1055 - 10572000年-

CoCrPtTa Single Layer Perpendicular Magnetic Recording Media with Carbon Underlayer

T. Onoue, A. Takizawa, T. Asahi, T. Osaka

Journal of Applied Physics88p.6645 - 66512000年-

Development of HAUP and Its Applications to Various Kinds of Solids

J. Kobayashi, T. Asahi

Proceedings of SPIE4097p.25 - 392000年-

Influence of Substrates on Magnetic Property and Crystalline Orientation of CoCrTa/TiCr Perpendicular Magnetic Recording Medium

T. Asahi, M. Ikeda, A. Takizawa, T. Onoue, T. Osaka

Journal of Magnetism & Magnetic Materials212p.293 - 2992000年-

Analysis of the Remanent Magnetization State at Track Edges for a Ring-Type Head and Single-Layered Perpendicular Magnetic Recording Medium

T. Hirayama, T. Onoue, T. Asahi, T. Honma, J. Hokkyo, T. Osaka

Journal of the Magnetics Society of Japan24p.223 - 2262000年-

Fabrication of a Single-Layered Perpendicular Magnetic Recording Medium with a Ge Underlayer

Y. Morita, K. Kuramochi, T. Onoue, T. Asahi, T. Osaka

Journal of the Magnetics Society of Japan24p.271 - 2742000年-

Preparation of a Perpendicular/Longitudinal Composite Medium with a Ni-Alloy Longitudinal Magnetic Underlayer

M. Kawakami, K. Kurasawa, M. Ikeda, T. Onoue, T. Asahi, T. Osaka

Journal of the Magnetics Society of Japan24p.263 - 2662000年-

Effect of a Paramagnetic CoCr40 Intermediate Layer on Magnetic Properties and Crystalline Structure of the CoCr18Pt6Ta3 Perpendicular Magnetic Recording Medium with an Amorphous C Underlayer

T. Asahi, T. Onoue, A. Takizawa, T. Osaka

Electrochemical Society Proceedings99-34p.304 - 3141999年-

Fabrication of a Perpendicular/Longitudinal Composite Medium by Sputtering

A. Takizawa, T. Onoue, M. Ikeda, T. Hirayama, T. Asahi, T. Osaka, H. Sakai

Journal of the Magnetics Society of Japan23p.973 - 9761999年-

Study on Medium Noise and Remanent Magnetization State for Single-Layer Perpendicular Magnetic Recording Media

T. Onoue, T. Asahi, T. Hirayama, J. Hokkyo, T. Osaka

Journal of the Magnetics Society of Japan23p.45 - 481999年-

Optical Study on Phase Transition of Pb0.92La0.08(Zr0.65Ti0.35) 0.89O3

T. Asahi, Y. Itagaki, H. Utsumi, H. Yoshii, I. Kamiya, M. Ichiki, M. Takahashi, J. Kobayashi

Ferroelectrics208p.459 - 4691998年-

The Optical Activity of Lysozyme Crystal

J. Kobayashi, T. Asahi, M. Sakurai, I. Kagomiya, H. Asai, H. Asami

Acta Crystallographica A54p.581 - 5901998年-

Optical Activity of Oxo Amide Crystal

T. Asahi, M. Nakamura, J. Kobayashi, F. Toda, H. Miyamoto

Journal of American Chemical Society119p.3665 - 36691997年-

The Optical Activity of Crystalline L-Aspartic Acids

T. Asahi, M. Takahashi, J. Kobayashi

Acta Crystallographica A53p.763 - 7711997年-

Thermal Expansion of Superconducting Bi2Sr2CaCu2O8

T. Asahi, H. Suzuki, M. Nakamura, H. Takano, J. Kobayashi

Physical Review B55p.9125 - 91291997年-

Optical Activity of Crystalline Glutamic Acids

T. Asahi, H. Utsumi, Y. Itagaki, I. Kagomiya, J. Kobayashi

Acta Crystallographica A52p.766 - 7691996年-

Optical Activity of Poly-L-Lactic Acids in Crystalline and Dissolved States

M. Ichiki, T. Asahi, J. Kobayashi

Phase Transitions56p.67 - 781996年-

Optical Properties of Superconducting Bi2Sr2CaCu2O8

J. Kobayashi, T. Asahi, M. Sakurai, M. Takahashi, K. Okubo, Y. Enomoto

Physical Review B53p.11784 - 117951996年-

Optical and X-ray Study on Phase Transition of (NH4)2SO4

T. Asahi, R. Ikeda, M. Higano, H. Suzuki, M. Nakamura, T. Morikawa, J. Kobayashi

Ferroelectrics Letters21p.47 - 531996年-

Optical Activity of Solid Polymer

J. Kobayashi, T. Asahi, M. Ichiki, A. Oikawa

Ferroelectrics171p.69 - 941995年-

Optical Study on Phase Transition of Lead Lanthanum Zirconate Titanate Pb0.92La0.08(Zr0.70Ti0.30)0.98O3 ceramics

J. Kobayashi, T. Asahi, M. Ichiki, K. Saito, T. Shimazaki, H. Yoshii, Y. Itagaki, H. Ikawa

Physical Review B51p.763 - 7781995年-

Structural and Optical Properties of Poly Lactic Acids

J. Kobayashi, T. Asahi, M. Ichiki, A. Oikawa, H. Suzuki, T. Watamabe, E. Fukada, Y. Shikinami

Journal of Applied Physics77p.2957 - 29731995年-

Optical Activity in Poly-L-Lactic Acid

J. Kobayashi, M. Ichiki, T. Watamabe, T. Asahi, A. Oikawa, H. Suzuki

Ferroelectrics151p.75 - 841994年-

Optical Activity of (NH4) 2SO4

M. Higano, T. Asahi, Y. Sato, K. Okubo, J. Kobayashi

Ferroelectrics152p.285 - 2871994年-

Optical Activity of Gd2 (MoO4) 3

K. Saito, T. Asahi, N. Takahashi, M. Higano, I. Kamiya, Y. Sato, K. Okubo, J. Kobayashi

Ferroelectrics152p.231 - 2361994年-

Optical Propreties of PLZT

M. Ichiki, T. Shimasaki, H. Yoshii, T. Asahi, J. Kobayashi, H. Ikawa

Ferroelectrics152p.289 - 2931994年-

Optical Activity and Phase Transitions of Pb1-xLax(ZryTiz) 1-x/4O3

H. Yoshii, T. Shimazaki, M. Ichiki, Y. Itagaki, T. Asahi, K. Saito, J. Kobayashi, H. Ikawa

Japanese Journal of Applied Physics33p.5435 - 54391994年-

Optical Properties of BaMnF4

T. Asahi, M. Tomizawa, J. Kobayashi, W. Kleemann

Physical Review B45p.1971 - 19871992年-

Optical Properties of Gd2 (MoO4) 3 Studied by High Accuracy Universal

T. Asahi, M. Higano, Cao Fu-Tian, J. Kobayashi

Japanese Journal of Applied Physics31p.3214 - 32171992年-

Optical Study of Pb1-xLax(ZryTiz) 1-x/4O3

M. Ichiki, T. Asahi, T. Shimasaki, J. Kobayashi, H. Ikawa

Japanese Journal of Applied Physics31p.3212 - 32131992年-

Optical and Structural Studies of Poly-Lactic Acid (I)

M. Ichiki, T. Asahi, T. Watanabe, J. Kobayashi, E. Fukada

Reports on Progress in Polymer Physics in Japan35p.441 - 4441992年-

Optical and Structural Studies of Poly-Lactic Acid (II)

M. Ichiki, T. Asahi, T. Watanabe, J. Kobayashi, E. Fukada

Reports on Progress in Polymer Physics in Japan35p.445 - 4481992年-

Optical Properties of Rochelle Salt

J. Kobayashi, K. Uchino, T. Asahi

Physical Review B43p.5706 - 57121991年-

Optical Activity of BaMnF4

T. Asahi, K. Uchino, M. Tomizawa, J. Kobayashi, W. Kleemann

Ferroelectrics105p.213 - 2171990年-

Optical Activity of Poly-L-Lactic Acid

T. Asahi, H. Fukase, M. Ichiki, J. Kobayashi, E. Fukada

Ferroelectrics109p.339 - 3421990年-

The Optical Study of BaMnF4 in Incommensurate Phase

T. Asahi, K. Uchino, J. Kobayashi, W. Kleemann

Ferroelectrics94p.329 - 3351989年-

Evaluation of the Systematic Errors of Polarimetric Measurements: Application to Measurement of the Gyration Tensor of α-Quartz

J. Kobayashi, T. Asahi, S. Takahashi, A. M. Glazer

Journal of Applied Crystallography21p.479 - 4841988年-

Simultaneous Measurement of Electrogyration and Electrooptic Effects ofα-Quartz

J. Kobayashi, T. Asahi, S. Takahashi

Ferroelectrics75p.139 - 1521987年-

A high-accuracy universal polarimeter study of optical anisotropy and optical activity in laminated collagen membranes

Kenta Nakagawa, Heather Harper-Lovelady, Yuji Tanaka, Masahito Tanaka, Masayuki Yamato, Toru Asahi

50(95)p.15086 - 15089

書籍等出版物

「異方性媒質のキラル光学/磁気光学の精密測定」

朝日透

磁気学会2013年-

ＣＳＪカレントレビュー13「キラル化学-その起源から最新のキラル材料研究まで」

朝日透，田中真人

化学同人2013年 10月-

生命科学概論

朝日透、池田康夫、石井義孝、井上貴文、大坂利文、大島登志男、岡村好子、合田亘人、澤村直哉、重谷安代、仙波憲太郎、武岡真司、武田直也、竹山春子、常田聡、増田優、南沢享

朝倉出版2012年-

Magnetic Materials, Processes, and Devices 9 : ECS Transaction Vol. 3 No.25

J. Sayama, Y. Yamashita, T. Asahi, T. Osaka

2007年-

Introduction to Complex Mediums for Optics and Electromagnetics SPIE PRESS

T. Asahi and J. Kobayashi

2003年-

SPIE Press

T. Asahi and J. Kobayashi

2003年-

今日からモノ知りシリーズ　トコトンやさしいナノテクノロジーの本

大泊巌（編著）

2002年 03月-

講演・口頭発表等

CeF3単結晶の光軸に垂直な方向のFaraday効果の正確な測定

中川鉄馬, 張堃, 朝日透

日本磁気学会学術講演会2018年09月13日

最適及びアンダードープBi2Sr2CaCu2O8+xの光学的性質と対称性の破れ

チョウコン, 松本匡貴, 中川鉄馬, 松田梓, 朝日透, 綿打敏司

日本物理学会 2018年秋季大会2018年09月10日

アミノ酸ドープ硫酸トリグリシン結晶の強誘電性とキラリティ

寺沢有果菜, 石川和彦, 一木正聡, 朝日透

日本物理学会 2018年秋季大会2018年09月10日

ビスチミン誘導体の自己集体内の光化学反応と形態変化

稲田萌花, 宇田川瑛弘, 齋藤敬, 小島秀子, 朝日透

2018年光化学討論会2018年09月07日

サリチリデンナフチルアミン結晶の光トリガー相転移

谷口卓也, 佐藤寛泰, 朝日透, 小島秀子

2018年光化学討論会2018年09月07日

Mechanical Motions of Salicylideneaniline Crystals Induced by Light and Heat

HAGIWARA Yuki, TANIGUCHI Takuya, KOSHIMA Hideko, ASAHI Toru

2018年光化学討論会2018年09月05日

Application of HAUPs to Amino Acid Crystals: Dedicated to Professor Jinzo Kobayashi

T.Asahi, K.Ishikawa, Y.Terasawa

4th Molecular Chirality Asia2018年07月06日

Chirality Control of Tryglycine Sulfate Crystals by Chiral Molecules

Yukana Terasawa, Kazuhiko Ishikawa, Masaaki Ichiki, Toru Asahi

4th Molecular Chirality Asia2018年07月05日

Thermal Locomotion of Chiral Crystal by Phase Transition

Takuya Taniguchi, Haruki Sugiyama, Hidehiro Uekusa, Motoo Shiro, Hideko Koshima, Toru Asahi

4th Molecular Chirality Asia2018年07月05日

Chiral Inversion of Thalidomide Enantiomers in their Powder State

Kanako Shinohara, Yuka Niizuma, Kohei Tanaka, Kohei Otogawa, Yoshiyuki Ogino, Fumiya Adachi, Toru Asahi

4th Molecular Chirality Asia2018年07月04日

Enhancement of Racemization in Powdered Thalidomide by Heating

Kanako Shinohara, Yuka Niizuma, Kohei Tanaka, Kohei Otogawa, Yoshiyuki Ogino, Fumiya Adachi, Toru Asahi

Chirality 20182018年06月12日

Relationship between Chirality and Ferroelectricity of Alanine doped Triglycine sulfate crystals

Yukana Terasawa, Kazuhiko Ishikawa, Masaaki Ichiki, Toru Asahi

RCBJSF 20182018年05月14日

アミノ酸をドープした硫酸鳥グリシン決勝の物性測定

楠⽥晃孝, 崎⼭菜緒, 寺沢有果菜, 朝⽇透

モレキュラー・キラリティー 20182018年05月12日

粉末状態サリドマイドエナンチオマーのキラル反転の解析

篠原加奈⼦, 新妻優花, ⽥中康平, ⼄川光平, 荻野禎之, ⾜⽴⾵⽔也, 朝⽇透

モレキュラー・キラリティー 20182018年05月11日

アラニンによる硫酸トリグリシン結晶のキラリティ制御機構

寺沢有果菜, ⽯川和彦, ⼀⽊正聡, 朝⽇透

モレキュラー・キラリティー 20182018年05月11日

アラニンによる強誘電性硫酸トリグリシン結晶のキラリティ制御

寺澤有果菜, 石川和彦, 一木正聡, 朝日透

2018年日本物理学会2018年03月23日

サリチリデンアニリン結晶における光屈曲の高速化

小島秀子, 小宮潤, 谷口卓也, 山中大樹, 古部昭広, 杉山晴紀, 植草秀裕, 佐藤寛泰, 朝日　透

日本化学会第98春季年会2018年03月22日

ジベンゾバレレン結晶の光屈曲

谷口卓也, 久保田あゆみ, 守時達也, 朝日透, 小島秀子

日本化学会第98春季年会2018年03月21日

Nafion塗布メソポーラスPd薄膜を用いた乳酸のセンシング

馬場大輔, 山内悠輔, 朝日透

日本化学会第98春季年会2018年03月20日

Thermal and Photo-triggered Phase Transition of Chiral Photochromic Salycilidenenaphthylethylamine crystal

TANIGUCHI, Takuya, SUGIMOTO, Ryota, KOMIYA, Jun, SATO, Hiroyasu, KOSHIMA, Hideko, ASAHI, Toru

日本化学会第98春季年会2018年03月20日

G-HAUP opens the door to condensed matter Chirality

T.Asahi

Toyota-Riken International Workshop2017年11月26日

Photo- and Thermo-induced Mechanical Motion of Aminosalicylideneaniline Crystals

J. Komiya・T. Taniguchi・D. Yamanaka・A. Furube・H. Koshima・T. Asahi

2017年光化学討論会2017年09月04日

Photochemical Reaction of Bis-Thymine Derivatives in Selfassembly

A. Udagawa, M. Inada, A. Al-Shereiqi, H. Koshima, K. Saito, T. Asahi

2017年光化学討論会2017年09月04日

Photo- and Thermal-induced Mechanical Motion of Chiral Salicylideneaniline Crystals

Taniguchi Takuya, Sugimoto Ryota, Komiya Jun, Koshima Hideko, Asahi Toru

2017年光化学討論会2017年09月04日

精神遅滞原因候補遺伝子cereblonの分子進化速度解析による機能的サイトの解明

小野寺航, 朝日透, 澤村直哉

第19回日本進化学会2017年08月26日

Cereblon promotes itochondrial degradation via autophagy in human neuroblastoma cells

Kosuke Kataoka, Toru Asahi, Naoya Sawamura

第60回日本神経化学会大会2017年08月03日

Optical Properties and Broken Symmetries in Bi2Sr2CaCu2O8+x

Kun Zhang, Masataka Matsumoto, Kenta Nakagawa, Satoshi Watauchi, Azusa Matsuda, Toru Asahi